Compounds and methods for modulating ube3a-ats

ABSTRACT

Provided are compounds, methods, and pharmaceutical compositions for reducing the amount or activity of UBE3A-ATS, the endogenous antisense transcript of ubiquitin protein ligase E3A (UBE3A) in a cell or subject, and in certain instances increasing the expression of paternal UBE3A and the amount of UBE3A protein in a cell or subject. 
     Such compounds, methods, and pharmaceutical compositions are useful to ameliorate at least one symptom or hallmark of a neurogenetic disorder. Such symptoms and hallmarks include developmental delays, ataxia, speech impairment, sleep problems, seizures, and EEG abnormalities. Such neurogenetic disorders include Angelman Syndrome.

SEQUENCE LISTING

The present application is being filed along with a Sequence Listing inelectronic format. The Sequence Listing is provided as a file entitledBIOL0349USC1SEQ_ST25.txt, created on Apr. 16, 2021, which is 1.87 MB insize. The information in the electronic format of the sequence listingis incorporated herein by reference in its entirety.

FIELD

Provided are compounds, methods, and pharmaceutical compositions forreducing the amount or activity of UBE3A-ATS, the endogenous antisensetranscript of ubiquitin protein ligase E3A (UBE3A) in a cell or subject,and in certain instances increasing the expression of paternal UBE3A andthe amount of UBE3A protein in a cell or subject. Such compounds,methods, and pharmaceutical compositions are useful to ameliorate atleast one symptom or hallmark of a neurogenetic disorder. Such symptomsand hallmarks include developmental delays, ataxia, speech impairment,sleep problems, seizures, and EEG abnormalities. Such neurogeneticdisorders include Angelman Syndrome.

BACKGROUND

Angelman Syndrome (AS) is a developmental disorder that affects˜1/15,000 live births and is caused by a deficiency of ubiquitin proteinligase E3A (UBE3A). Of the two copies of the UBE3A gene in neurons, thematernal gene is typically expressed, while the paternal gene is subjectto genetic imprinting and silencing. Angelman Syndrome is caused when afunctional copy of the UBE3A gene is not inherited from the mother,either because of a mutation, a deletion, paternal uniparental disomy ofchromosome 15, or an imprinting defect. See, Buiting, et al., “AngelmanSyndrome—insights into a rare neurogenetic disorder”, Nature Rev.Neuro., 2016, 12:584-593.

Angelman Syndrome patients experience developmental delays and speechimpairment, and commonly experience sleep problems, seizures, and EEGabnormalities. The disorder is usually diagnosed in the first few yearsof life and the diagnosis can be confirmed by genetic testing. However,therapies for Angelman Syndrome remain limited and focus mainly onsymptomatic management. See, Williams, C. A. et al., Genet. Med., 12:385-395, 2010.

Recently, topoisomerase inhibitors currently used in cancer treatmentwere found to “unsilence” paternal UBE3A expression in both a neuronalculture system and mice. See, Huang, H. S. et al., Nature, 481: 185-189,2012. However, the exact mechanism of unsilencing paternal UBE3Aexpression remains unknown and topoisomerase inhibitors are fraught withsafety concerns because they are known to be non-specific and capable ofinducing DNA damage, such as single and double-strand breaks.

Currently there is a lack of acceptable options for treatingneurogenetic disorders such as AS. It is therefore an object herein toprovide compounds, methods, and pharmaceutical compositions for thetreatment of such diseases.

SUMMARY OF THE INVENTION

Provided herein are compounds, methods and pharmaceutical compositionsfor reducing the amount or activity of UBE3A-ATS RNA, and in certainembodiments increasing the expression of paternal UBE3A RNA or proteinin a cell or subject. In certain embodiments, the subject has aneurogenetic disorder. In certain embodiments, the subject has AngelmanSyndrome (AS). In certain embodiments, compounds useful for reducingexpression of UBE3A-ATS RNA, or a portion thereof, are oligomericcompounds. In certain embodiments, compounds useful for reducingexpression of UBE3A-ATS RNA, or a portion thereof, are modifiedoligonucleotides. In certain embodiments, compounds useful forincreasing expression of paternal UBE3A RNA or protein are oligomericcompounds In certain embodiments, compounds useful for increasingexpression of paternal UBE3A RNA or protein are modifiedoligonucleotides.

Also provided are methods useful for ameliorating at least one symptomor hallmark of a neurogenetic disorder. In certain embodiments, theneurogenetic disorder is Angelman Syndrome. In certain embodiments, thesymptom or hallmark includes developmental delays, ataxia, speechimpairment, sleep problems, seizures, and/or EEG abnormalities.

DETAILED DESCRIPTION OF THE INVENTION

It is to be understood that both the foregoing general description andthe following detailed description are exemplary and explanatory onlyand are not restrictive. Herein, the use of the singular includes theplural unless specifically stated otherwise. As used herein, the use of“or” means “and/or” unless stated otherwise. Furthermore, the use of theterm “including” as well as other forms, such as “includes” and“included”, is not limiting. Also, terms such as “element” or“component” encompass both elements and components comprising one unitand elements and components that comprise more than one subunit, unlessspecifically stated otherwise.

The section headings used herein are for organizational purposes onlyand are not to be construed as limiting the subject matter described.All documents, or portions of documents, cited in this application,including, but not limited to, patents, patent applications, articles,books, and treatises, are hereby expressly incorporated-by-reference forthe portions of the document discussed herein, as well as in theirentirety.

Definitions

Unless specific definitions are provided, the nomenclature used inconnection with, and the procedures and techniques of, analyticalchemistry, synthetic organic chemistry, and medicinal and pharmaceuticalchemistry described herein are those well-known and commonly used in theart. Where permitted, all patents, applications, published applicationsand other publications and other data referred to throughout in thedisclosure are incorporated by reference herein in their entirety.

Unless otherwise indicated, the following terms have the followingmeanings:

Definitions

As used herein, “2′-deoxynucleoside” means a nucleoside comprising a2′-H(H) deoxyribosyl sugar moiety. In certain embodiments, a2′-deoxynucleoside is a 2′-β-D-deoxynucleoside and comprises a2′-β-D-deoxyribosyl sugar moiety, which has the β-D configuration asfound in naturally occurring deoxyribonucleic acids (DNA). In certainembodiments, a 2′-deoxynucleoside may comprise a modified nucleobase ormay comprise an RNA nucleobase (uracil).

As used herein, “2′-MOE” or “2′-MOE sugar moiety” means a 2′-OCH₂CH₂OCH₃group in place of the 2′-OH group of a ribosyl sugar moiety. “MOE” meansmethoxyethyl. Unless otherwise indicated, a 2′-MOE has the β-Dstereochemical configuration.

As used herein, “2′-MOE nucleoside” means a nucleoside comprising a2′-MOE sugar moiety.

As used herein, “2′-OMe” or “2′-O-methyl sugar moiety” means a 2′-OCH₃group in place of the 2′-OH group of a ribosyl sugar moiety. Unlessotherwise indicated, a 2′-OMe has the β-D stereochemical configuration.

As used herein, “2′-OMe nucleoside” means a nucleoside comprising a2′-OMe sugar moiety.

As used herein, “2′-substituted nucleoside” means a nucleosidecomprising a 2′-substituted sugar moiety. As used herein,“2′-substituted” in reference to a sugar moiety means a sugar moietycomprising at least one 2¹-substituent group other than H or OH.

As used herein, “5-methyl cytosine” means a cytosine modified with amethyl group attached to the 5 position. A 5-methyl cytosine is amodified nucleobase.

As used herein, “administering” means providing a pharmaceutical agentto a subject.

As used herein, “antisense activity” means any detectable and/ormeasurable change attributable to the hybridization of an antisensecompound to its target nucleic acid. In certain embodiments, antisenseactivity is a decrease in the amount or expression of a target nucleicacid or protein encoded by such target nucleic acid compared to targetnucleic acid levels or target protein levels in the absence of theantisense compound.

As used herein, “antisense compound” means an oligomeric compoundcapable of achieving at least one antisense activity.

As used herein, “ameliorate” in reference to a treatment meansimprovement in at least one symptom relative to the same symptom in theabsence of the treatment. In certain embodiments, amelioration is thereduction in the severity or frequency of a symptom or the delayed onsetor slowing of progression in the severity or frequency of a symptom. Incertain embodiments, the symptom or hallmark is developmental delays,ataxia, speech impairment, sleep problems, seizures, and EEGabnormalities.

As used herein, “bicyclic nucleoside” or “BNA” means a nucleosidecomprising a bicyclic sugar moiety.

As used herein, “bicyclic sugar” or “bicyclic sugar moiety” means amodified sugar moiety comprising two rings, wherein the second ring isformed via a bridge connecting two of the atoms thereby forming abicyclic structure. In certain embodiments, the first ring of thebicyclic sugar moiety is a furanosyl moiety. In certain embodiments, thebicyclic sugar moiety does not comprise a furanosyl moiety.

As used herein, “cleavable moiety” means a bond or group of atoms thatis cleaved under physiological conditions, for example, inside a cell, asubject, or a human.

As used herein, “complementary” in reference to an oligonucleotide meansthat at least 70% of the nucleobases of the oligonucleotide or one ormore portions thereof and the nucleobases of a target nucleic acid orone or more portions thereof are capable of hydrogen bonding with oneanother when the nucleobase sequence of the oligonucleotide and theother nucleic acid are aligned in opposing directions. As used herein,complementary nucleobases means nucleobases that are capable of forminghydrogen bonds with one another. Complementary nucleobase pairs includeadenine (A) and thymine (T), adenine (A) and uracil (U), cytosine (C)and guanine (G), 5-methyl cytosine (mC) and guanine (G). Complementaryoligonucleotides and/or target nucleic acids need not have nucleobasecomplementarity at each nucleoside. Rather, some mismatches aretolerated. As used herein, “fully complementary” or “100% complementary”in reference to an oligonucleotide, or portion thereof, means that theoligonucleotide, or portion thereof, is complementary to anotheroligonucleotide or target nucleic acid at each nucleobase of the shorterof the two oligonucleotides, or at each nucleoside if theoligonucleotides are the same length.

As used herein, “conjugate group” means a group of atoms that isdirectly or indirectly attached to an oligonucleotide. Conjugate groupsinclude a conjugate moiety and a conjugate linker that attaches theconjugate moiety to the oligonucleotide.

As used herein, “conjugate linker” means a single bond or a group ofatoms comprising at least one bond that connects a conjugate moiety toan oligonucleotide.

As used herein, “conjugate moiety” means a group of atoms that isattached to an oligonucleotide via a conjugate linker.

As used herein, “contiguous” in the context of an oligonucleotide refersto nucleosides, nucleobases, sugar moieties, or internucleoside linkagesthat are immediately adjacent to each other. For example, “contiguousnucleobases” means nucleobases that are immediately adjacent to eachother in a sequence.

As used herein, “constrained ethyl” or “cEt” or “cEt modified sugarmoiety” means a β-D ribosyl bicyclic sugar moiety wherein the secondring of the bicyclic sugar is formed via a bridge connecting the4′-carbon and the 2′-carbon of the β-D ribosyl sugar moiety, wherein thebridge has the formula 4′-CH(CH₃)—O-2′, and wherein the methyl group ofthe bridge is in the S configuration.

As used herein, “cEt nucleoside” means a nucleoside comprising a cEtmodified sugar moiety.

As used herein, “chirally enriched population” means a plurality ofmolecules of identical molecular formula, wherein the number orpercentage of molecules within the population that contain a particularstereochemical configuration at a particular chiral center is greaterthan the number or percentage of molecules expected to contain the sameparticular stereochemical configuration at the same particular chiralcenter within the population if the particular chiral center werestereorandom. Chirally enriched populations of molecules having multiplechiral centers within each molecule may contain one or more stereorandomchiral centers. In certain embodiments, the molecules are modifiedoligonucleotides. In certain embodiments, the molecules are compoundscomprising modified oligonucleotides.

As used herein, “gapmer” means a modified oligonucleotide comprising aninternal region having a plurality of nucleosides that support RNase Hcleavage positioned between external regions having one or morenucleosides, wherein the nucleosides comprising the internal region arechemically distinct from the nucleoside or nucleosides comprising theexternal regions. The internal region may be referred to as the “gap”and the external regions may be referred to as the “wings.” Unlessotherwise indicated, “gapmer” refers to a sugar motif. Unless otherwiseindicated, the sugar moiety of each nucleoside of the gap is a2′-β-D-deoxyribosyl sugar moiety. Thus, the term “MOE gapmer” indicatesa gapmer having a gap comprising 2′-β-D-deoxynucleosides and wingscomprising 2′-MOE nucleosides. Unless otherwise indicated, a MOE gapmermay comprise one or more modified internucleoside linkages and/ormodified nucleobases and such modifications do not necessarily followthe gapmer pattern of the sugar modifications.

As used herein, “hotspot region” is a range of nucleobases on a targetnucleic acid that is amenable to oligomeric compound-mediated reductionof the amount or activity of the target nucleic acid.

As used herein, “hybridization” means the pairing or annealing ofcomplementary oligonucleotides and/or nucleic acids. While not limitedto a particular mechanism, the most common mechanism of hybridizationinvolves hydrogen bonding, which may be Watson-Crick, Hoogsteen orreversed Hoogsteen hydrogen bonding, between complementary nucleobases.

As used herein, “internucleoside linkage” means the covalent linkagebetween contiguous nucleosides in an oligonucleotide. As used herein,“modified internucleoside linkage” means any internucleoside linkageother than a phosphodiester internucleoside linkage. “Phosphorothioateinternucleoside linkage” is a modified internucleoside linkage in whichone of the non-bridging oxygen atoms of a phosphodiester internucleosidelinkage is replaced with a sulfur atom.

As used herein, “linker-nucleoside” means a nucleoside that links,either directly or indirectly, an oligonucleotide to a conjugate moiety.Linker-nucleosides are located within the conjugate linker of anoligomeric compound. Linker-nucleosides are not considered part of theoligonucleotide portion of an oligomeric compound even if they arecontiguous with the oligonucleotide.

As used herein, “non-bicyclic modified sugar moiety” means a modifiedsugar moiety that comprises a modification, such as a substituent, thatdoes not form a bridge between two atoms of the sugar to form a secondring.

As used herein, “mismatch” or “non-complementary” means a nucleobase ofa first oligonucleotide that is not complementary with the correspondingnucleobase of a second oligonucleotide or target nucleic acid when thefirst and second oligonucleotide are aligned.

As used herein, “motif” means the pattern of unmodified and/or modifiedsugar moieties, nucleobases, and/or internucleoside linkages, in anoligonucleotide.

As used herein, “neurogenetic disorder” means a condition of the nervoussystem caused by a heritable genetic factor, including, but not limitedto, a mutation, a deletion, uniparental disomy, or an imprinting defect.

As used herein, “nucleobase” means an unmodified nucleobase or amodified nucleobase. As used herein an “unmodified nucleobase” isadenine (A), thymine (T), cytosine (C), uracil (U), or guanine (G). Asused herein, a “modified nucleobase” is a group of atoms other thanunmodified A, T, C, U, or G capable of pairing with at least oneunmodified nucleobase. A “5-methyl cytosine” is a modified nucleobase. Auniversal base is a modified nucleobase that can pair with any one ofthe five unmodified nucleobases. As used herein, “nucleobase sequence”means the order of contiguous nucleobases in a target nucleic acid oroligonucleotide independent of any sugar or internucleoside linkagemodification.

As used herein, “nucleoside” means a compound comprising a nucleobaseand a sugar moiety. The nucleobase and sugar moiety are each,independently, unmodified or modified. As used herein, “modifiednucleoside” means a nucleoside comprising a modified nucleobase and/or amodified sugar moiety. Modified nucleosides include abasic nucleosides,which lack a nucleobase. “Linked nucleosides” are nucleosides that areconnected in a contiguous sequence (i.e., no additional nucleosides arepresented between those that are linked).

As used herein, “oligomeric compound” means an oligonucleotide andoptionally one or more additional features, such as a conjugate group orterminal group. An oligomeric compound may be paired with a secondoligomeric compound that is complementary to the first oligomericcompound or may be unpaired. A “singled-stranded oligomeric compound” isan unpaired oligomeric compound. The term “oligomeric duplex” means aduplex formed by two oligomeric compounds having complementarynucleobase sequences. Each oligomeric compound of an oligomeric duplexmay be referred to as a “duplexed oligomeric compound.”

As used herein, “oligonucleotide” means a strand of linked nucleosidesconnected via internucleoside linkages, wherein each nucleoside andinternucleoside linkage may be modified or unmodified. Unless otherwiseindicated, oligonucleotides consist of 8-50 linked nucleosides. As usedherein, “modified oligonucleotide” means an oligonucleotide, wherein atleast one nucleoside or internucleoside linkage is modified. As usedherein, “unmodified oligonucleotide” means an oligonucleotide that doesnot comprise any nucleoside modifications or internucleosidemodifications.

As used herein, “pharmaceutically acceptable carrier or diluent” meansany substance suitable for use in administering to a subject. Certainsuch carriers enable pharmaceutical compositions to be formulated as,for example, tablets, pills, dragees, capsules, liquids, gels, syrups,slurries, suspension and lozenges for the oral ingestion by a subject.In certain embodiments, a pharmaceutically acceptable carrier or diluentis sterile water, sterile saline, sterile buffer solution or sterileartificial cerebrospinal fluid.

As used herein, “pharmaceutically acceptable salts” meansphysiologically and pharmaceutically acceptable salts of compounds.Pharmaceutically acceptable salts retain the desired biological activityof the parent compound and do not impart undesired toxicological effectsthereto.

As used herein, “pharmaceutical composition” means a mixture ofsubstances suitable for administering to a subject. For example, apharmaceutical composition may comprise an oligomeric compound and asterile aqueous solution. In certain embodiments, a pharmaceuticalcomposition shows activity in free uptake assay in certain cell lines.

As used herein, “prodrug” means a therapeutic agent in a form outsidethe body that is converted to a different form within a subject or cellsthereof. Typically, conversion of a prodrug within the subject isfacilitated by the action of an enzymes (e.g., endogenous or viralenzyme) or chemicals present in cells or tissues and/or by physiologicconditions.

As used herein, “reducing or inhibiting the amount or activity”,“reducing the amount or activity”, or “inhibiting the amount oractivity” refers to a reduction or blockade of the transcriptionalexpression or activity relative to the transcriptional expression oractivity in an untreated or control sample and does not necessarilyindicate a total elimination of transcriptional expression or activity.

As used herein, “RNA” means any RNA transcript, including endogenousantisense transcripts that do not encode a protein (e.g., UBE3A-ATS),and also includes pre-mRNA and mature mRNA unless otherwise specified.

As used herein, “RNAJ₁ compound” means an antisense compound that acts,at least in part, through RISC or Ago2 to modulate a target nucleic acidand/or protein encoded by a target nucleic acid. RNAJ₁ compoundsinclude, but are not limited to double-stranded siRNA, single-strandedRNA (ssRNA), and microRNA, including microRNA mimics In certainembodiments, an RNAJ₁ compound modulates the amount, activity, and/orsplicing of a target nucleic acid. The term RNAJ₁ compound excludesantisense compounds that act through RNase H.

As used herein, “self-complementary” in reference to an oligonucleotidemeans an oligonucleotide that at least partially hybridizes to itself.

As used herein, “standard cell assay” means the assay described inExample 1 and reasonable variations thereof.

As used herein, “stereorandom chiral center” in the context of apopulation of molecules of identical molecular formula means a chiralcenter having a random stereochemical configuration. For example, in apopulation of molecules comprising a stereorandom chiral center, thenumber of molecules having the (5) configuration of the stereorandomchiral center may be but is not necessarily the same as the number ofmolecules having the (R) configuration of the stereorandom chiralcenter. The stereochemical configuration of a chiral center isconsidered random when it is the result of a synthetic method that isnot designed to control the stereochemical configuration. In certainembodiments, a stereorandom chiral center is a stereorandomphosphorothioate internucleoside linkage.

As used herein, “subject” means a human or non-human animal

As used herein, “sugar moiety” means an unmodified sugar moiety or amodified sugar moiety. As used herein, “unmodified sugar moiety” means a2′-OH(H) β-D-ribosyl moiety, as found in RNA (an “unmodified RNA sugarmoiety”), or a 2′-H(H) β-D-deoxyribosyl sugar moiety, as found in DNA(an “unmodified DNA sugar moiety”). Unmodified sugar moieties have onehydrogen at each of the 1′, 3′, and 4′ positions, an oxygen at the 3′position, and two hydrogens at the 5′ position. As used herein,“modified sugar moiety” or “modified sugar” means a modified furanosylsugar moiety or a sugar surrogate.

As used herein, “sugar surrogate” means a modified sugar moiety havingother than a furanosyl moiety that can link a nucleobase to anothergroup, such as an internucleoside linkage, conjugate group, or terminalgroup in an oligonucleotide. Modified nucleosides comprising sugarsurrogates can be incorporated into one or more positions within anoligonucleotide and such oligonucleotides are capable of hybridizing tocomplementary oligomeric compounds or target nucleic acids.

As used herein, “symptom or hallmark” means any physical feature or testresult that indicates the existence or extent of a disease or disorder.In certain embodiments, a symptom is apparent to a subject or to amedical professional examining or testing said subject. In certainembodiments, a hallmark is apparent upon invasive diagnostic testing,including, but not limited to, post-mortem tests.

As used herein, “target nucleic acid” and “target RNA” mean a nucleicacid that an antisense compound is designed to affect.

As used herein, “target region” means a portion of a target nucleic acidto which an oligomeric compound is designed to hybridize.

As used herein, “terminal group” means a chemical group or group ofatoms that is covalently linked to a terminus of an oligonucleotide.

As used herein, “therapeutically effective amount” means an amount of apharmaceutical agent that provides a therapeutic benefit to a subject.For example, a therapeutically effective amount improves a symptom orhallmark of a disease.

Certain Embodiments The Present Disclosure Provides the FollowingNon-Limiting Numbered Embodiments:

Embodiment 1. An oligomeric compound comprising a modifiedoligonucleotide consisting of 12 to 30 linked nucleosides wherein thenucleobase sequence of the modified oligonucleotide is at least 90%complementary to an equal length portion of a UBE3A-ATS RNA, and whereinthe modified oligonucleotide comprises at least one modificationselected from a modified sugar moiety and a modified internucleosidelinkage.

Embodiment 2. An oligomeric compound comprising a modifiedoligonucleotide consisting of 12 to 30 linked nucleosides and having anucleobase sequence comprising at least 12, 13, 14, 15, 16, 17, 18, 19,or 20 contiguous nucleobases of any of SEQ ID NOs: 17-2762, 2786-2863,2872-2904.

Embodiment 3. An oligomeric compound comprising a modifiedoligonucleotide consisting of 12 to 30 linked nucleosides and having anucleobase sequence comprising at least 12, 13, 14, 15, 16, 17, or 18contiguous nucleobases of any of SEQ ID NOs: 2763-2785 or 2864-2871.

Embodiment 4. An oligomeric compound comprising a modifiedoligonucleotide consisting of 12 to 30 linked nucleosides and having anucleobase sequence comprising at least 8, at least 9, at least 10, atleast 11, at least 12, at least 13, at least 14, at least 15, at least16, at least 17, at least 18, at least 19, or at least 20 contiguousnucleobases complementary to:

-   -   an equal length portion of nucleobases 461,413-461,487 of SEQ ID        NO: 1;    -   an equal length portion of nucleobases 468,968-469,013 of SEQ ID        NO: 1; or    -   an equal length portion of nucleobases 483,965-484,003 of SEQ ID        NO: 1

Embodiment 5. An oligomeric compound comprising a modifiedoligonucleotide consisting of 12 to 30 linked nucleosides and having anucleobase sequence comprising at least 8, at least 9, at least 10, atleast 11, at least 12, at least 13, at least 14, at least 15, at least16, at least 17, or at least 18 contiguous nucleobases of a sequenceselected from:

-   -   SEQ ID Nos:1053, 1329, 1501, 1576, 1873, 1949, 2025, 2096, 2245,        2512,2591,2680-2682, and 2844;    -   SEQ ID Nos: 376, 377, 2751-2756, 2773-2776, 2872, 2873,        2876-2878; or    -   SEQ ID Nos: 172, 764-770, 995, 1445, 1668, 1743, 2255, 2595,        2762-2767.

Embodiment 6. The oligomeric compound of any of embodiments 1-5, whereinthe modified oligonucleotide has a nucleobase sequence that is at least80%, 85%, 90%, 95%, or 100% complementary to the nucleobase sequence ofSEQ ID NO: 1, SEQ ID NO: 2915, or SEQ ID NO: 2916 when measured acrossthe entire nucleobase sequence of the modified oligonucleotide.

Embodiment 7. The oligomeric compound of any of embodiments 1-6, whereinthe modified oligonucleotide comprises at least one modified nucleoside.

Embodiment 8. The oligomeric compound of embodiment 7, wherein themodified oligonucleotide comprises at least one modified nucleosidecomprising a modified sugar moiety.

Embodiment 9. The oligomeric compound of embodiment 8, wherein themodified oligonucleotide comprises at least one modified nucleosidecomprising a bicyclic sugar moiety.

Embodiment 10. The oligomeric compound of embodiment 9, wherein themodified oligonucleotide comprises at least one modified nucleosidecomprising a bicyclic sugar moiety having a 2′-4′ bridge, wherein the2′-4′ bridge is selected from -0-CH₂-; and -O-CH(CH₃)-.

Embodiment 11. The oligomeric compound of any of embodiments 7-10,wherein the modified oligonucleotide comprises at least one modifiednucleoside comprising a non-bicyclic modified sugar moiety.

Embodiment 12. The oligomeric compound of embodiment 11, wherein thenon-bicyclic modified sugar moiety is a 2′-MOE modified sugar moiety or2′-OMe modified sugar moiety.

Embodiment 13. The oligomeric compound of any of embodiments 7-8,wherein the modified oligonucleotide comprises at least one modifiednucleoside comprising a sugar surrogate.

Embodiment 14. The oligomeric compound of embodiment 13, wherein themodified oligonucleotide comprises at least one modified nucleosidecomprising a sugar surrogate selected from morpholino and PNA.

Embodiment 15. The oligomeric compound of any of embodiments 1-8 or11-14, wherein the modified oligonucleotide does not comprise a bicyclicsugar moiety.

Embodiment 16. The oligomeric compound of any of embodiments 1-15,wherein the modified oligonucleotide is a gapmer.

Embodiment 17. The oligomeric compound of any of embodiments 1-16,wherein the modified oligonucleotide has a sugar motif comprising:

-   -   a 5′-region consisting of 1-6 linked 5′-region nucleosides;    -   a central region consisting of 6-10 linked central region        nucleosides; and    -   a 3′-region consisting of 1-6 linked 3′-region nucleosides;        wherein each of the 5′-region nucleosides and each of the        3′-region nucleosides comprises a modified sugar moiety and each        of the central region nucleosides comprises a        2′-β-D-deoxyribosyl sugar moiety.

Embodiment 18. The oligomeric compound of embodiment 17, wherein themodified oligonucleotide has

-   -   a 5′-region consisting of 5 linked 5′-region nucleosides;    -   a central region consisting of 10 linked central region        nucleosides; and    -   a 3′-region consisting of 5 linked 3′-region nucleosides;        wherein each of the 5′-region nucleosides and each of the        3′-region nucleosides comprises a 2′-MOE modified sugar moiety,        and each of the central region nucleosides comprises a        2′-β-D-deoxyribosyl sugar moiety.

Embodiment 19. The oligomeric compound of embodiment 17, wherein themodified oligonucleotide has

-   -   a 5′-region consisting of 4 linked 5′-region nucleosides;    -   a central region consisting of 10 linked central region        nucleosides; and    -   a 3′-region consisting of 6 linked 3′-region nucleosides;        wherein each of the 5′-region nucleosides and each of the        3′-region nucleosides a 2′-MOE modified sugar moiety, and each        of the central region nucleosides comprises a        2′-β-D-deoxyribosyl sugar moiety.

Embodiment 20. The oligomeric compound of embodiment 17, wherein themodified oligonucleotide has

-   -   a 5′-region consisting of 6 linked 5′-region nucleosides;    -   a central region consisting of 10 linked central region        nucleosides; and    -   a 3′-region consisting of 4 linked 3′-region nucleosides;        wherein each of the 5′-region nucleosides and each of the        3′-region nucleosides a 2′-MOE modified sugar moiety, and each        of the central region nucleosides comprises a        2′-β-D-deoxyribosyl sugar moiety.

Embodiment 21. The oligomeric compound of embodiment 17, wherein themodified oligonucleotide has

-   -   a 5′-region consisting of 5 linked 5′-region nucleosides;    -   a central region consisting of 8 linked central region        nucleosides; and    -   a 3′-region consisting of 5 linked 3′-region nucleosides;        wherein each of the 5′-region nucleosides and each of the        3′-region nucleosides a 2′-MOE modified sugar moiety, and each        of the central region nucleosides comprises a        2′-β-D-deoxyribosyl sugar moiety.

Embodiment 22. The oligomeric compound of embodiment 17, wherein themodified oligonucleotide has

-   -   a 5′-region consisting of 4 linked 5′-region nucleosides;    -   a central region consisting of 8 linked central region        nucleosides; and    -   a 3′-region consisting of 6 linked 3′-region nucleosides;        wherein each of the 5′-region nucleosides and each of the        3′-region nucleosides a 2′-MOE modified sugar moiety, and each        of the central region nucleosides comprises a        2′-β-D-deoxyribosyl sugar moiety.

Embodiment 23. The oligomeric compound of embodiment 17, wherein themodified oligonucleotide has

-   -   a 5′-region consisting of 4 linked 5′-region nucleosides;    -   a central region consisting of 8 linked central region        nucleosides; and    -   a 3′-region consisting of 6 linked 3′-region nucleosides;        wherein each of the 5′-region nucleosides and each of the        3′-region nucleosides a 2′-MOE modified sugar moiety, and each        of the central region nucleosides comprises a        2′-β-D-deoxyribosyl sugar moiety.

Embodiment 24. The oligomeric compound of any of embodiments 1-23,wherein the modified oligonucleotide comprises at least one modifiedinternucleoside linkage.

Embodiment 25. The oligomeric compound of embodiment 24, wherein eachinternucleoside linkage of the modified oligonucleotide is a modifiedinternucleoside linkage.

Embodiment 26. The oligomeric compound of embodiment 24 or 25 whereinthe modified internucleoside linkage is a phosphorothioateinternucleoside linkage.

Embodiment 27. The oligomeric compound of embodiment 24 or 26 whereinthe modified oligonucleotide comprises at least one phosphodiesterinternucleoside linkage.

Embodiment 28. The oligomeric compound of any of embodiments 24, 26, or27, wherein each internucleoside linkage is independently selected froma phosphodiester internucleoside linkage or a phosphorothioateinternucleoside linkage.

Embodiment 29. The oligomeric compound of embodiments 1-24 or 27-28,wherein the modified oligonucleotide has an internucleoside linkagemotif selected from among: soooossssssssssooss, sooooossssssssssoss,soooosssssssssoss, sooosssssssssooss, sooossssssssssoooss, orsoosssssssssoooss; wherein,

s=a phosphorothioate internucleoside linkage and o =a phosphodiesterinternucleoside linkage.

Embodiment 30. The oligomeric compound of any of embodiments 1-29,wherein the modified oligonucleotide comprises a modified nucleobase.

Embodiment 31. The oligomeric compound of embodiment 30, wherein themodified nucleobase is a 5-methyl cytosine.

Embodiment 32. The oligomeric compound of any of embodiments 1-31,wherein the modified oligonucleotide consists of 12-30, 12-22,12-20,14-18, 14-20, 15-17, 15-25, 16-20, 18-22 or 18-20 linkednucleosides.

Embodiment 33. The oligomeric compound of any of embodiments 1-32,wherein the modified oligonucleotide consists of 18 linked nucleosides.

Embodiment 34. The oligomeric compound of any of embodiments 1-2, 4, or6-32, wherein the modified oligonucleotide consists of 20 linkednucleosides.

Embodiment 35. The oligomeric compound of any of embodiments 1-34,consisting of the modified oligonucleotide.

Embodiment 36. The oligomeric compound of any of embodiments 1-34,comprising a conjugate group comprising a conjugate moiety and aconjugate linker.

Embodiment 37. The oligomeric compound of embodiment 36, wherein theconjugate linker consists of a single bond.

Embodiment 38. The oligomeric compound of embodiment 36, wherein theconjugate linker is cleavable.

Embodiment 39. The oligomeric compound of embodiment 36, wherein theconjugate linker comprises 1-3 linker-nucleosides.

Embodiment 40. The oligomeric compound of any of embodiments 36-39,wherein the conjugate group is attached to the modified oligonucleotideat the 5′-end of the modified oligonucleotide.

Embodiment 41. The oligomeric compound of any of embodiments 36-39,wherein the conjugate group is attached to the modified oligonucleotideat the 3′-end of the modified oligonucleotide.

Embodiment 42. The oligomeric compound of any of embodiments 1-34 or36-41, comprising a terminal group.

Embodiment 43. The oligomeric compound of any of embodiments 1-42wherein the oligomeric compound is a singled-stranded oligomericcompound.

Embodiment 44. The oligomeric compound of any of embodiments 1-38 or40-43, wherein the oligomeric compound does not compriselinker-nucleosides.

Embodiment 45. An oligomeric duplex comprising an oligomeric compound ofany of embodiments 1-42 or 44.

Embodiment 46. An antisense compound comprising or consisting of anoligomeric compound of any of embodiments 1-44 or an oligomeric duplexof embodiment 45.

Embodiment 47. A pharmaceutical composition comprising an oligomericcompound of any of embodiments 1-44 or an oligomeric duplex ofembodiment 45 and a pharmaceutically acceptable carrier or diluent.

Embodiment 48. The pharmaceutical composition of embodiment 47, whereinthe pharmaceutically acceptable diluent is artificial cerebral spinalfluid.

Embodiment 49. The pharmaceutical composition of embodiment 48, whereinthe pharmaceutical composition consists essentially of the modifiedoligonucleotide and phosphate buffered saline.

Embodiment 50. A method comprising administering to a subject apharmaceutical composition of any of embodiments 47-50.

Embodiment 51. A method of treating a disease associated with UBE3A-ATScomprising administering to an individual having or at risk fordeveloping a disease associated with UBE3A-ATS a therapeuticallyeffective amount of a pharmaceutical composition according to any ofembodiments 47-49; and thereby treating the disease associated withUBE3A-ATS.

Embodiment 52. The method of embodiment 51, wherein theUBE3A-ATS-associated disease is Angelman Syndrome.

Embodiment 53. The method of any of embodiments 50-52, wherein at leastone symptom or hallmark of the UBE3A-ATS-associated disease isameliorated.

Embodiment 54. The method of embodiment 53, wherein the symptom orhallmark is developmental delays, ataxia, speech impairment, sleepproblems, seizures, or EEG abnormalities.

Embodiment 55. A modified oligonucleotide according to the followingchemical structure:

or a salt thereof.

Embodiment 56. A modified oligonucleotide according to the followingchemical structure:

Embodiment 57. A modified oligonucleotide according to the followingchemical structure:

or a salt thereof.

Embodiment 58. A modified oligonucleotide according to the followingchemical structure:

Embodiment 59. A modified oligonucleotide according to the followingchemical structure:

or a salt thereof.

Embodiment 60. A modified oligonucleotide corresponding to the followingchemical structure:

Embodiment 61. A modified oligonucleotide according to the followingchemical structure:

or a salt thereof.

Embodiment 62. A modified oligonucleotide according to the followingchemical structure:

Embodiment 63. A modified oligonucleotide according to the followingchemical structure:

or a salt thereof.

Embodiment 64. A modified oligonucleotide according to the followingchemical structure:

Embodiment 65. A modified oligonucleotide according to the followingchemical structure:

or a salt thereof.

Embodiment 66. A modified oligonucleotide according to the followingchemical structure:

Embodiment 67. The modified oligonucleotide of any of embodiments 55,57, 59, 61, 63, or 65, which is a sodium salt of the chemical structure.

Embodiment 68. A pharmaceutical composition comprising the modifiedoligonucleotide of any of embodiments 55-67 and a pharmaceuticallyacceptable carrier or diluent.

Embodiment 69. The pharmaceutical composition of embodiment 68, whereinthe pharmaceutically acceptable diluent is artificial cerebrospinalfluid.

Embodiment 70. The pharmaceutical composition of embodiment 69, whereinthe pharmaceutical composition consists essentially of the modifiedoligonucleotide and artificial cerebrospinal fluid.

Embodiment 71. A compound comprising a modified oligonucleotideaccording to the following chemical notation: ^(m)C_(es)A_(eo)T_(eo)^(m)C_(eo)A_(eo)T_(ds)G_(ds)A_(ds)T_(ds)^(m)C_(ds)T_(ds)T_(ds)G_(ds)G_(ds)T_(ds)A_(eo)A_(eo)G_(es)G_(es)^(m)C_(e) (SEQ ID NO: 1949), wherein:

A=an adenine nucleobase,

mC=a 5-methyl cytosine nucleobase,

G=a guanine nucleobase,

T=a thymine nucleobase,

e=a 2′-MOE sugar moiety,

d=a 2′-β-D-deoxyribosyl sugar moiety,

s=a phosphorothioate internucleoside linkage, and

o=a phosphodiester internucleoside linkage.

Embodiment 72. A compound comprising a modified oligonucleotideaccording to the following chemical notation: T_(es) ^(m)C_(eo)A_(eo)^(m)C_(eo) ^(m)C_(eo)A_(eo)T_(ds)T_(ds)T_(ds)T_(ds)G_(ds)A_(ds)^(m)C_(ds) ^(m)C_(ds)T_(ds)T_(ds) ^(m)C_(eo)T_(es)T_(es)A_(e)(SEQ ID NO:2751), wherein:

A=an adenine nucleobase,

mC=a 5-methyl cytosine nucleobase,

G=a guanine nucleobase,

T=a thymine nucleobase,

e=a 2′-MOE sugar moiety,

d=a 2′-β-D-deoxyribosyl sugar moiety,

s=a phosphorothioate internucleoside linkage, and

o=a phosphodiester internucleoside linkage.

Embodiment 73. A compound comprising a modified oligonucleotideaccording to the following chemical notation: T_(es)T_(eo)^(m)C_(eo)A_(eo) ^(m)C_(eo)^(m)C_(eo)A_(ds)T_(ds)T_(ds)T_(ds)T_(ds)G_(ds)A_(ds) ^(m)C_(ds)^(m)C_(ds)T_(ds)T_(eo) ^(m)C_(es)T_(es)T_(e)(SEQ ID NO: 2752), wherein:

A=an adenine nucleobase,

mC=a 5-methyl cytosine nucleobase,

G=a guanine nucleobase,

T=a thymine nucleobase,

e=a 2′-MOE sugar moiety,

d=a 2′-β-D-deoxyribosyl sugar moiety,

s=a phosphorothioate internucleoside linkage, and

o=a phosphodiester internucleoside linkage.

Embodiment 74. A compound comprising a modified oligonucleotideaccording to the following chemical notation: G_(es)^(m)C_(eo)A_(eo)T_(eo)A_(eo) ^(m)C_(eo) ^(m)C_(ds)^(m)C_(ds)A_(ds)G_(ds)G_(ds)G_(ds)T_(ds)A_(ds)G_(ds)G_(ds)A_(eo)T_(es)T_(es)^(m)C_(e)(SEQ ID NO: 765), wherein:

A=an adenine nucleobase,

mC=a 5-methyl cytosine nucleobase,

G=a guanine nucleobase,

T=a thymine nucleobase,

e=a 2′-MOE sugar moiety,

d=a 2′-β-D-deoxyribosyl sugar moiety,

s=a phosphorothioate internucleoside linkage, and

o=a phosphodiester internucleoside linkage.

Embodiment 75. A compound comprising a modified oligonucleotideaccording to the following chemical notation: A_(es) ^(m)C_(eo)G_(eo)^(m)C_(es)A_(ds)A_(ds)T_(ds)G_(ds)T_(ds)A_(ds)T_(ds)^(m)C_(ds)A_(eo)G_(eo)G_(eo) ^(m)C_(es)A_(es)A_(e) (SEQ ID NO: 2866),wherein:

A=an adenine nucleobase,

mC=a 5-methyl cytosine nucleobase,

G=a guanine nucleobase,

T=a thymine nucleobase,

e=a 2′-MOE sugar moiety,

d=a 2′-β-D-deoxyribosyl sugar moiety,

s=a phosphorothioate internucleoside linkage, and

o=a phosphodiester internucleoside linkage.

Embodiment 76. A compound comprising a modified oligonucleotideaccording to the following chemical notation: A_(es) ^(m)C_(eo)^(m)C_(eo)A_(eo)T_(eo)T_(ds)T_(ds)T_(ds)G_(ds)A_(ds) ^(m)C_(ds)^(m)C_(ds)T_(ds)T_(ds) ^(m)C_(ds)T_(eo)T_(eo)A_(es)G_(es) ^(m)C_(e)(SEQID NO: 2873), wherein:

A=an adenine nucleobase,

mC=a 5-methyl cytosine nucleobase,

G=a guanine nucleobase,

T=a thymine nucleobase,

e=a 2′-MOE sugar moiety,

d=a 2′-β-D-deoxyribosyl sugar moiety,

s=a phosphorothioate internucleoside linkage, and

o=a phosphodiester internucleoside linkage.

Embodiment 77. The compound of any of embodiments 71-76, comprising themodified oligonucleotide covalently linked to a conjugate group.

Embodiment 78. A pharmaceutical composition of any of embodiments 71-77,and a pharmaceutically acceptable diluent or carrier.

Embodiment 79. The pharmaceutical composition of embodiment 78, whereinthe pharmaceutically acceptable diluent is artificial cerebrospinalfluid.

Embodiment 80. The pharmaceutical composition of embodiment 79, whereinthe pharmaceutical composition consists essentially of the modifiedoligonucleotide and artificial cerebrospinal fluid.

Embodiment 81. A chirally enriched population of modifiedoligonucleotides of any of embodiments 55-67, wherein the population isenriched for modified oligonucleotides comprising at least oneparticular phosphorothioate internucleoside linkage having a particularsetereochemical configuration.

Embodiment 82. The chirally enriched population of embodiment 81,wherein the population is enriched for modified oligonucleotidescomprising at least one particular phorphorothioate internucleosidelinkage having the (Sp) configuration.

Embodiment 83. The chirally enriched population of embodiment 81,wherein the population is enriched for modified oligonucleotidescomprising at least one particular phorphorothioate internucleosidelinkage having the (Rp) configuration.

Embodiment 84. The chirally enriched population of embodiment 81,wherein the population is enriched for modified oligonucleotides havinga particular, independently selected stereochemical configuration ateach phosphorothioate internucleoside linkage

Embodiment 85. The chirally enriched population of embodiment 84,wherein the population is enriched for modified oligonucleotides havingthe (Sp) configuration at each phosphorothioate internucleoside linkage.

Embodiment 86. The chirally enriched population of embodiment 84,wherein the population is enriched for modified oligonucleotides havingthe (Rp) configuration at each phosphorothioate internucleoside linkage.

Embodiment 87. The chirally enriched population of embodiment 84,wherein the population is enriched for modified oligonucleotides havingthe (Rp) configuration at one particular phosphorothioateinternucleoside linkage and the (Sp) configuration at each of theremaining phosphorothioate internucleoside linkages.

Embodiment 88. The chirally enriched population of embodiment 81 orembodiment 84 wherein the population is enriched for modifiedoligonucleotides having at least 3 contiguous phosphorothioateinternucleoside linkages in the Sp, Sp, and Rp configurations, in the 5′to 3′ direction.

Embodiment 89. A chirally enriched population of modifiedoligonucleotides of any of embodiments 55-67, wherein all of thephosphorothioate internucleoside linkages of the modifiedoligonucleotide are stereorandom.

I. Certain Oligonucleotides

In certain embodiments, provided herein are oligomeric compoundscomprising oligonucleotides, which consist of linked nucleosides.Oligonucleotides may be unmodified oligonucleotides (RNA or DNA) or maybe modified oligonucleotides. Modified oligonucleotides comprise atleast one modification relative to unmodified RNA or DNA. That is,modified oligonucleotides comprise at least one modified nucleoside(comprising a modified sugar moiety and/or a modified nucleobase) and/orat least one modified internucleoside linkage.

A. Certain Modified Nucleosides

Modified nucleosides comprise a modified sugar moiety or a modifiednucleobase or both a modifed sugar moiety and a modified nucleobase.

1. Certain Sugar Moieties

In certain embodiments, modified sugar moieties are non-bicyclicmodified sugar moieties. In certain embodiments, modified sugar moietiesare bicyclic or tricyclic sugar moieties. In certain embodiments,modified sugar moieties are sugar surrogates. Such sugar surrogates maycomprise one or more substitutions corresponding to those of other typesof modified sugar moieties.

In certain embodiments, modified sugar moieties are non-bicyclicmodified sugar moieties comprising a furanosyl ring with one or moresubstituent groups none of which bridges two atoms of the fumnosyl ringto form a bicyclic structure. Such non bridging substituents may be atany position of the furanosyl, including but not limited to substituentsat the 2′, 4′, and/or 5′ positions. In certain embodiments one or morenon-bridging substituent of non-bicyclic modified sugar moieties isbranched. Examples of 2′-substituent groups suitable for non-bicyclicmodified sugar moieties include but are not limited to: 2′-F, 2′-OCH₃(“OMe” or “O-methyl”), and 2′-0(CH₂)₂OCH₃ (“MOE”). In certainembodiments, 2′-substituent groups are selected from among: halo, allyl,amino, azido, SH, CN, OCN, CF₃, OCF₃, O—C₁-C₁₀ alkoxy, O—C₁-C₁₀substituted alkoxy, O—C₁-C₁₀ alkyl, O—C₁-C₁₀ substituted alkyl, S-alkyl,N(R_(m))-alkyl, O-alkenyl, S-alkenyl, N(R_(m))-alkenyl, O-alkynyl,S-alkynyl, N(R_(m))-alkynyl, O-alkylenyl-O-alkyl, alkynyl, alkaryl,aralkyl, O-alkaryl, O-aralkyl, O(CH₂)₂SCH₃, O(CH₂)₂ON(R_(m))(R_(n)) orOCH₂C(═O)—N(R_(m))(R_(n)), where each R_(m) and R_(n) is, independently,H, an amino protecting group, or substituted or unsubstituted C₁-C₁₀alkyl, and the 2′-substituent groups described in Cook et al., U.S. Pat.No. 6,531,584; Cook et al., U.S. Pat. No. 5,859,221; and Cook et al.,U.S. Pat. No. 6,005,087. Certain embodiments of these 2′-substituentgroups can be further substituted with one or more substituent groupsindependently selected from among: hydroxyl, amino, alkoxy, carboxy,benzyl, phenyl, nitro (NO₂), thiol, thioalkoxy, thioalkyl, halogen,alkyl, aryl, alkenyl and alkynyl. Examples of 4′-substituent groupssuitable for non-bicyclic modified sugar moieties include but are notlimited to alkoxy (e.g., methoxy), alkyl, and those described inManoharan et al., WO 2015/106128. Examples of 5′-substituent groupssuitable for non-bicyclic modified sugar moieties include but are notlimited to: 5-methyl (R or S), 5′-vinyl, and 5′-methoxy. In certainembodiments, non-bicyclic modified sugar moieties comprise more than onenon-bridging sugar substituent, for example, 2′-F-5′-methyl sugarmoieties and the modified sugar moieties and modified nucleosidesdescribed in Migawa et al., WO 2008/101157 and Rajeev et al.,US2013/0203836.

In certain embodiments, a 2′-substituted non-bicyclic modifiednucleoside comprises a sugar moiety comprising a non-bridging2′-substituent group selected from: F, NH₂, N₃, OCF_(3,) OCH₃,O(CH₂)₃NH₂, CH₂CH═CH₂, OCH₂CH═CH₂, OCH₂CH₂OCH₃, O(CH₂)₂SCH₃,O(CH₂)₂ON(R_(m))(R_(n)), O(CH₂)₂O(CH₂)₂N(CH₃)₂, and N-substitutedacetamide (OCH₂C(═O)—N(R_(m))(R_(n))), where each R_(m) and R_(n) is,independently, H, an amino protecting group, or substituted orunsubstituted C₁-C₁₀ alkyl.

In certain embodiments, a 2′-substituted nucleoside non-bicyclicmodified nucleoside comprises a sugar moiety comprising a non-bridging2′-substituent group selected from: F, OCF₃, OCH₃, OCH₂CH₂OCH₃,O(CH₂)₂SCH₃, O(CH₂)₂ON(CH₃)₂, O(CH₂)₂O(CH₂)₂N(CH₃)₂, andOCH₂C(═O)—N(H)CH₃ (“NMA”).

In certain embodiments, a 2′-substituted non-bicyclic modifiednucleoside comprises a sugar moiety comprising a non-bridging2′-substituent group selected from: F, OCH₃, and OCH₂CH₂OCH₃.

Certain modifed sugar moieties comprise a substituent that bridges twoatoms of the furanosyl ring to form a second ring, resulting in abicyclic sugar moiety. In certain such embodiments, the bicyclic sugarmoiety comprises a bridge between the 4′ and the 2′ furanose ring atoms.Examples of such 4′ to 2′ bridging sugar substituents include but arenot limited to: 4′-CH₂-2′, 4′-(CH₂)₂-2′, 4′-(CH₂)₃-2′, 4′-CH₂—O-2′(“LNA”), 4′-(CH₂)₂-O-2′ (“ENA”), 4′-CH(CH₃)—O-2′ (referred to as“constrained ethyl” or “cEt”), 4′-CH₂—O—CH₂-2′, 4′-CH₂—N(R)-2′,4′-CH(CH₂OCH₃)—O-2′ (“constrained MOE” or “cMOE”) and analogs thereof(see, e.g., Seth et al., U.S. Pat. No. 7,399,845, Bhat et al., U.S. Pat.No. 7,569,686, Swayze et al., U.S. Pat. No. 7,741,457, and Swayze etal., U.S. Pat. No. 8,022,193), 4′-C(CH₃)(CH₃)—O-2′ and analogs thereof(see, e.g., Seth et al., U.S. Pat. No. 8,278,283), 4′-CH₂—N(OCH₃)-2′ andanalogs thereof (see, e.g., Prakash et al., U.S. Pat. No. 8,278,425),4′-CH₂—O—N(CH₃)-2′ (see, e.g., Allerson et al., U.S. Pat. No. 7,696,345and Allerson et al., U.S. Pat. No. 8,124,745), 4′-CH₂—C(H)(CH₃)-2′ (see,e.g., Zhou, et al., J. Org. Chem.,2009, 74, 118-134), 4′-CH₂—C(═CH₂)-2′and analogs thereof (see e.g., Seth et al., U.S. Pat. No. 8,278,426),4′-C(R_(a)R_(b))—N(R)—O-2′, 4′-C(R_(a)R_(b))—O—N(R)-2′,4′-CH₂—O—N(R)-2′, and 4′-CH₂—N(R)—O- 2′, wherein each R, R_(a), andR_(b) is, independently, H, a protecting group, or C₁-C₁₂ alkyl (see,e.g. Imanishi et al., U.S. Pat. No. 7,427,672).

In certain embodiments, such 4′ to 2′ bridges independently comprisefrom 1 to 4 linked groups independently selected from:—[C(R_(a))(R_(b))]_(n)—, —[C(R_(a))(R_(b))]_(n)—O—, —C(R_(a))═C(R_(b))—,—C(R_(a))═N—, —C(═NR_(a))—, —C(═O)—, —C(═S)—, —O—, —Si(R_(a))₂—,—S(═O)_(x)—, and —N(R_(a))—;

wherein:

x is 0, 1, or 2;

n is 1, 2, 3, or 4;

each R_(a) and R_(b) is, independently, H, a protecting group, hydroxyl,C₁-C₁₂ alkyl, substituted C₁-C₁₂ alkyl, C₂-C₁₂ alkenyl, substitutedC₂-C₁₂ alkenyl, C₂-C₁₂ alkynyl, substituted C₂-C₁₂ alkynyl, C₅-C₂₀ aryl,substituted C₅-C₂₀ aryl, heterocycle radical, substituted heterocycleradical, heteroaryl, substituted heteroaryl, C₅-C₇ alicyclic radical,substituted C₅-C₇ alicyclic radical, halogen, OJ₁, NJ₁J₂, SJ₁, N₃,COOJ₁, acyl (C(═O)—H), substituted acyl, CN, sulfonyl (S(═O)₂-J₁), orsulfoxyl (S(═O)-J₁); and

each J₁ and J2 is, independently, H, C₁-C₁₂ alkyl, substituted C₁-C₁₂alkyl, C₂-C₁₂ alkenyl, substituted C₂-C₁₂ alkenyl, C₂-C₁₂ alkynyl,substituted C₂-C₁₂ alkynyl, C₅-C₂₀ aryl, substituted C₅-C₂₀ aryl, acyl(C(═O)—H), substituted acyl, a heterocycle radical, a substitutedheterocycle radical, C₁-C₁₂ aminoalkyl, substituted C₁-C₁₂ aminoalkyl,or a protecting group.

Additional bicyclic sugar moieties are known in the art, see, forexample: Freier et al., Nucleic Acids Research, 1997, 25(22), 4429-4443,Albaek et al., J. Org. Chem., 2006, 71, 7731-7740, Singh et al., Chem.Commun., 1998, 4, 455-456; Koshkin et al., Tetrahedron, 1998, 54,3607-3630; Kumar et al., Bioorg. Med. Chem. Lett., 1998, 8, 2219-2222;Singh et al., J. Org. Chem., 1998, 63, 10035-10039; Srivastava et al.,J. Am. Chem. Soc., 2007, 129, 8362-8379;Wengel et a., U.S. Pat. No.7,053,207; Imanishi et al., U.S. Pat. No. 6,268,490; Imanishi et al.U.S. Pat. No.6,770,748; Imanishi et al., U.S. RE44,779; Wengel et al.,U.S. Pat. No. 6,794,499; Wengel et al., U.S. Pat. No. 6,670,461; Wengelet al., U.S. Pat. No. 7,034,133; Wengel et al., U.S. Pat. No. 8,080,644;Wengel et al., U.S. Pat. No. 8,034,909; Wengel et al., U.S. Pat. No.8,153,365; Wengel et al., U.S. Pat. No. 7,572,582; Ramasamy et al., U.S.Pat. No. 6,525,191; Torsten et al., WO 2004/106356; Wengel et al., WO1999/014226; Seth et al., WO 2007/134181; Seth et al., U.S. Pat. No.7,547,684; Seth et al., U.S. Pat. No. 7,666,854; Seth et al., U.S. Pat.No. 8,088,746; Seth et al., U.S. Pat. No. 7,750,131; Seth et al., U.S.Pat. No. 8,030,467; Seth et al., U.S. Pat. No. 8,268,980; Seth et al.,U.S. Pat. No. 8,546,556; Seth et al., U.S. Pat. No. 8,530,640; Migawa etal., U.S. Pat. No. 9,012,421; Seth et al., U.S. Pat. No. 8,501,805; andU.S. Patent Publication Nos. Allerson et al., US2008/0039618 and Migawaet al., US2015/0191727.

In certain embodiments, bicyclic sugar moieties and nucleosidesincorporating such bicyclic sugar moieties are further defined byisomeric configuration. For example, an LNA nucleoside (describedherein) may be in the α-L configuration or in the β-D configuration.

α-L-methyleneoxy (4′-CH₂—O-2′) or α-L-LNA bicyclic nucleosides have beenincorporated into oligonucleotides that showed antisense activity(Frieden et al., Nucleic Acids Research, 2003, 21, 6365-6372). Herein,general descriptions of bicyclic nucleosides include both isomericconfigurations. When the positions of specific bicyclic nucleosides(e.g., LNA or cEt) are identified in exemplified embodiments herein,they are in the β-D configuration, unless otherwise specified.

In certain embodiments, modified sugar moieties comprise one or morenon-bridging sugar substituent and one or more bridging sugarsubstituent (e.g., 5′-substituted and 4′-2′ bridged sugars).

In certain embodiments, modified sugar moieties are sugar surrogates. Incertain such embodiments, the oxygen atom of the sugar moiety isreplaced, e.g., with a sulfur, carbon or nitrogen atom. In certain suchembodiments, such modified sugar moieties also comprise bridging and/ornon-bridging substituents as described herein. For example, certainsugar surrogates comprise a 4′-sulfur atom and a substitution at the2¹-position (see, e.g., Bhat et al., U.S. Pat. No. 7,875,733 and Bhat etal., U.S. 7,939,677) and/or the 5′ position.

In certain embodiments, sugar surrogates comprise rings having otherthan 5 atoms. For example, in certain embodiments, a sugar surrogatecomprises a six-membered tetrahydropyran (“THP”). Such tetrahydropyransmay be further modified or substituted. Nucleosides comprising suchmodified tetrahydropyrans include but are not limited to hexitol nucleicacid (“HNA”), anitol nucleic acid (“ANA”), manitol nucleic acid (“MNA”)(see, e.g., Leumann, C J. Bioorg. & Med. Chem. 2002, 10, 841-854),fluoro HNA:

(“F-HNA”, see e.g. Swayze et al., U.S. Pat. No. 8,088,904; Swayze etal., U.S. Pat. No. 8,440,803; Swayze et al., U.S. Pat. No. 8,796,437;and Swayze et al., U.S. Pat. No. 9,005,906; F-HNA can also be referredto as a F-THP or 3¹-fluoro tetrahydropyran), and nucleosides comprisingadditional modified THP compounds having the formula:

wherein, independently, for each of said modified THP nucleoside:

Bx is a nucleobase moiety;

T₃ and T₄ are each, independently, an internucleoside linking grouplinking the modified THP nucleoside to the remainder of anoligonucleotide or one of T₃ and T₄ is an internucleoside linking grouplinking the modified THP nucleoside to the remainder of anoligonucleotide and the other of T₃ and T₄ is H, a hydroxyl protectinggroup, a linked conjugate group, or a 5′ or 3′-terminal group;

-   q₁, q₂, q₃, q₄, q₅, q₆ and q₇ are each, independently, H, C₁-C₆    alkyl, substituted C₁-C₆ alkyl, C₂-C₆ alkenyl, substituted C₂- C₆    alkenyl, C₂-C₆ alkynyl, or substituted C₂-C₆ alkynyl; and

each of R₁ and R₂ is independently selected from among: hydrogen,halogen, substituted or unsubstituted alkoxy, NJ₁J₂, SJ₁, N₃, OC(═X)J₁,OC(═X)NJ₁J₂, NJ₃C(═X)NJ₁J₂, and CN, wherein X is O, S or NJ₁, and eachJ₁, J₂, and J₃ is, independently, H or C₁-C₆ alkyl.

In certain embodiments, modified THP nucleosides are provided whereinq₁, q₂, q₃, q₄, q₅, q₆ and q₇ are each H. In certain embodiments, atleast one of q₁, q₂, q₃, q₄, q₅, q₆ and q₇ is other than H. In certainembodiments, at least one of q₁, q_(2,) q₃, q₄, q₅, q₆ and q₇ is methyl.In certain embodiments, modified THP nucleosides are provided whereinone of R₁ and R₂ is F. In certain embodiments, R₁ is F and R₂ is H, incertain embodiments, R₁ is methoxy and R₂ is H, and in certainembodiments, R₁ is methoxyethoxy and R₂ is H.

In certain embodiments, sugar surrogates comprise rings having more than5 atoms and more than one heteroatom. For example, nucleosidescomprising morpholino sugar moieties and their use in oligonucleotideshave been reported (see, e.g., Braasch et al., Biochemistry, 2002, 41,4503-4510 and Summerton et al., U.S. Pat. No. 5,698,685; Summerton etal., U.S. Pat. No. 5,166,315; Summerton et al., U.S. Pat. No. 5,185,444;and Summerton et al., U.S. Pat. No. 5,034,506). As used here, the term“morpholino” means a sugar surrogate having the following structure:

In certain embodiments, morpholinos may be modified, for example byadding or altering various substituent groups from the above morpholinostructure. Such sugar surrogates are referred to herein as “modifedmorpholinos.”

In certain embodiments, sugar surrogates comprise acyclic moieites.Examples of nucleosides and oligonucleotides comprising such acyclicsugar surrogates include but are not limited to: peptide nucleic acid(“PNA”), acyclic butyl nucleic acid (see, e.g., Kumar et al., Org.Biomol. Chem., 2013, 11, 5853-5865), and nucleosides andoligonucleotides described in Manoharan et al., WO2011/133876.

Many other bicyclic and tricyclic sugar and sugar surrogate ring systemsare known in the art that can be used in modified nucleosides.

2. Certain Modified Nucleobases

In certain embodiments, modified oligonucleotides comprise one or morenucleosides comprising an unmodified nucleobase. In certain embodiments,modified oligonucleotides comprise one or more nucleosides comprising amodified nucleobase. In certain embodiments, modified oligonucleotidescomprise one or more nucleosides that does not comprise a nucleobase,referred to as an abasic nucleoside.

In certain embodiments, modified nucleobases are selected from:5-substituted pyrimidines, 6-azapyrimidines, alkyl or alkynylsubstituted pyrimidines, alkyl substituted purines, and N-2, N-6 and O-6substituted purines. In certain embodiments, modified nucleobases areselected from: 2-aminopropyladenine, 5-hydroxymethyl cytosine, xanthine,hypoxanthine, 2-aminoadenine, 6-N-methylguanine, 6-N-methyladenine,2-propyladenine, 2-thiouracil, 2-thiothymine and 2-thiocytosine,5-propynyl (—C≡C—CH₃) uracil, 5-propynylcytosine, 6-azouracil,6-azocytosine, 6-azothymine, 5-ribosyluracil (pseudouracil),4-thiouracil, 8-halo, 8-amino, 8-thiol, 8-thioalkyl, 8-hydroxyl, 8-azaand other 8-substituted purines, 5-halo, particularly 5-bromo,5-trifluoromethyl, 5-halouracil, and 5-halocytosine, 7-methylguanine,7-methyladenine, 2-F-adenine, 2-aminoadenine, 7-deazaguanine,7-deazaadenine, 3-deazaguanine, 3-deazaadenine, 6-N-benzoyladenine,2-N-isobutyrylguanine, 4-N-benzoylcytosine, 4-N-benzoyluracil, 5-methyl4-N-benzoylcytosine, 5-methyl 4-N-benzoyluracil, universal bases,hydrophobic bases, promiscuous bases, size-expanded bases, andfluorinated bases. Further modified nucleobases include tricyclicpyrimidines, such as 1,3-diazaphenoxazine-2-one,1,3-diazaphenothiazine-2-one and9-(2-aminoethoxy)-1,3-diazaphenoxazine-2-one (G-clamp) Modifiednucleobases may also include those in which the purine or pyrimidinebase is replaced with other heterocycles, for example 7-deaza-adenine,7-deazaguanosine, 2-aminopyridine and 2-pyridone. Further nucleobasesinclude those disclosed in Merigan et al., U.S. Pat. No. 3,687,808,those disclosed in The Concise Encyclopedia Of Polymer Science AndEngineering, Kroschwitz, J. I., Ed., John Wiley & Sons, 1990, 858-859;Englisch et al., Angewandte Chemie, International Edition, 1991, 30,613; Sanghvi, Y.S., Chapter 15, Antisense Research and Applications,Crooke, S. T. and Lebleu, B., Eds., CRC Press, 1993, 273-288; and thosedisclosed in Chapters 6 and 15, Antisense Drug Technology, Crooke S. T.,Ed., CRC Press, 2008, 163-166 and 442-443.

Publications that teach the preparation of certain of the above notedmodified nucleobases as well as other modified nucleobases includewithout limitation, Manoharan et al., US2003/0158403; Manoharan et al.,US2003/0175906; Dinh et al., U.S. Pat. No. 4,845,205; Spielvogel et al.,U.S. Pat. No. 5,130,302; Rogers et al., U.S. Pat. No. 5,134,066;Bischofberger et al., U.S. Pat. No. 5,175,273; Urdea et al., U.S. Pat.No. 5,367,066; Benner et al., U.S. Pat. No. 5,432,272; Matteucci et al.,U.S. Pat. No. 5,434,257; Gmeiner et al., U.S. Pat. No. 5,457,187; Cooket al., U.S. Pat. No. 5,459,255; Froehler et al., U.S. Pat. No.5,484,908; Matteucci et al., U.S. Pat. No. 5,502,177; Hawkins et al.,U.S. Pat. No. 5,525,711; Haralambidis et al., U.S. Pat. No. 5,552,540;Cook et al., U.S. Pat. No. 5,587,469; Froehler et al., U.S. Pat. No.5,594,121; Switzer et al., U.S. Pat. No. 5,596,091; Cook et al., U.S.Pat. No. 5,614,617; Froehler et al., U.S. Pat. No. 5,645,985; Cook etal., U.S. Pat. No. 5,681,941; Cook et al., U.S. Pat. No. 5,811,534; Cooket al., U.S. Pat. No. 5,750,692; Cook et al., U.S. Pat. No. 5,948,903;Cook et al., U.S. Pat. No. 5,587,470; Cook et al., U.S. Pat. No.5,457,191; Matteucci et al., U.S. Pat. No. 5,763,588; Froehler et al.,U.S. Pat. No. 5,830,653; Cook et al., U.S. Pat. No. 5,808,027; Cook etal., U.S. Pat. No. 6,166,199; and Matteucci et al., U.S. Pat. No.6,005,096.

3. Certain Modified Internucleoside Linkages

In certain embodiments, nucleosides of modified oligonucleotides may belinked together using any internucleoside linkage. The two main classesof internucleoside linking groups are defined by the presence or absenceof a phosphorus atom. Representative phosphorus-containinginternucleoside linkages include but are not limited to phosphodiesters,which contain a phosphodiester bond (“P(O₂)═O”) (also referred to asunmodified or naturally occurring linkages), phosphotriesters,methylphosphonates, phosphoramidates, and phosphorothioates (“P(O₂)═S”),and phosphorodithioates (“HS-P═S”). Representative non-phosphoruscontaining internucleoside linking groups include but are not limited tomethylenemethylimino (—CH₂—N(CH₃)—O—CH₂—), thiodiester, thionocarbamate(—O—C(═O)(NH)—S—); siloxane (—O—SH₂—O—); and N,N′-dimethylhydrazine(—CH₂—N(CH₃)—N(CH₃)—). Modified internucleoside linkages, compared tonaturally occurring phosphate linkages, can be used to alter, typicallyincrease, nuclease resistance of the oligonucleotide. In certainembodiments, internucleoside linkages having a chiral atom can beprepared as a racemic mixture, or as separate enantiomers. Methods ofpreparation of phosphorous-containing and non-phosphorous-containinginternucleoside linkages are well known to those skilled in the art.

Representative internucleoside linkages having a chiral center includebut are not limited to alkylphosphonates and phosphorothioates. Modifiedoligonucleotides comprising internucleoside linkages having a chiralcenter can be prepared as populations of modified oligonucleotidescomprising stereorandom internucleoside linkages, or as populations ofmodified oligonucleotides comprising phosphorothioate linkages inparticular stereochemical configurations. In certain embodiments,populations of modified oligonucleotides comprise phosphorothioateinternucleoside linkages wherein all of the phosphorothioateinternucleoside linkages are stereorandom. Such modifiedoligonucleotides can be generated using synthetic methods that result inrandom selection of the stereochemical configuration of eachphosphorothioate linkage. Nonetheless, as is well understood by those ofskill in the art, each individual phosphorothioate of each individualoligonucleotide molecule has a defined stereoconfiguration. In certainembodiments, populations of modified oligonucleotides are enriched formodified oligonucleotides comprising one or more particularphosphorothioate internucleoside linkages in a particular, independentlyselected stereochemical configuration. In certain embodiments, theparticular configuration of the particular phosphorothioate linkage ispresent in at least 65% of the molecules in the population. In certainembodiments, the particular configuration of the particularphosphorothioate linkage is present in at least 70% of the molecules inthe population. In certain embodiments, the particular configuration ofthe particular phosphorothioate linkage is present in at least 80% ofthe molecules in the population. In certain embodiments, the particularconfiguration of the particular phosphorothioate linkage is present inat least 90% of the molecules in the population. In certain embodiments,the particular configuration of the particular phosphorothioate linkageis present in at least 99% of the molecules in the population. Suchchirally enriched populations of modified oligonucleotides can begenerated using synthetic methods known in the art, e.g., methodsdescribed in Oka et al., JACS 125, 8307 (2003), Wan et al. Nuc. Acid.Res. 42, 13456 (2014), and WO 2017/015555. In certain embodiments, apopulation of modified oligonucleotides is enriched for modifiedoligonucleotides having at least one indicated phosphorothioate in the(Sp) configuration. In certain embodiments, a population of modifiedoligonucleotides is enriched for modified oligonucleotides having atleast one phosphorothioate in the (Rp) configuration. In certainembodiments, modified oligonucleotides comprising (Rp) and/or (Sp)phosphorothioates comprise one or more of the following formulas,respectively, wherein “B” indicates a nucleobase:

Unless otherwise indicated, chiral internucleoside linkages of modifiedoligonucleotides described herein can be stereorandom or in a particularstereochemical configuration.

Neutral internucleoside linkages include, without limitation,phosphotriesters, methylphosphonates, MMI (3′-CH₂—N(CH₃)—O-5′), amide-3(3′-CH₂—C(═O)—N(H)-5′), amide-4 (3′-CH₂—N(H)—C(═O)-5′), formacetal(3′-O—CH₂—O—5′), methoxypropyl (MOP), and thioformacetal(3′-S—CH₂—O-5′). Further neutral internucleoside linkages includenonionic linkages comprising siloxane (dialkylsiloxane), carboxylateester, carboxamide, sulfide, sulfonate ester and amides (See forexample: Carbohydrate Modifications in Antisense Research; Y. S. Sanghviand P. D. Cook, Eds., ACS Symposium Series 580; Chapters 3 and 4,40-65). Further neutral internucleoside linkages include nonioniclinkages comprising mixed N, O, S and CH₂ component parts.

B. Certain Motifs

In certain embodiments, modified oligonucleotides comprise one or moremodified nucleosides comprising a modified sugar moiety. In certainembodiments, modified oligonucleotides comprise one or more modifiednucleosides comprising a modified nucleobase. In certain embodiments,modified oligonucleotides comprise one or more modified internucleosidelinkage. In such embodiments, the modified, unmodified, and differentlymodified sugar moieties, nucleobases, and/or internucleoside linkages ofa modified oligonucleotide define a pattern or motif. In certainembodiments, the patterns of sugar moieties, nucleobases, andinternucleoside linkages are each independent of one another. Thus, amodified oligonucleotide may be described by its sugar motif, nucleobasemotif and/or internucleoside linkage motif (as used herein, nucleobasemotif describes the modifications to the nucleobases independent of thesequence of nucleobases).

1. Certain Sugar Motifs

In certain embodiments, oligonucleotides comprise one or more type ofmodified sugar and/or unmodified sugar moiety arranged along theoligonucleotide or portion thereof in a defined pattern or sugar motif.In certain instances, such sugar motifs include but are not limited toany of the sugar modifications discussed herein.

In certain embodiments, modified oligonucleotides have a gapmer motif,which is defined by two external regions or “wings” and a central orinternal region or “gap.” The three regions of a gapmer motif (the5′-wing, the gap, and the 3′-wing) form a contiguous sequence ofnucleosides wherein at least some of the sugar moieties of thenucleosides of each of the wings differ from at least some of the sugarmoieties of the nucleosides of the gap. Specifically, at least the sugarmoieties of the nucleosides of each wing that are closest to the gap(the 3′-most nucleoside of the 5′-wing and the 5′-most nucleoside of the3′-wing) differ from the sugar moiety of the neighboring gapnucleosides, thus defining the boundary between the wings and the gap(i.e., the wing/gap junction). In certain embodiments, the sugarmoieties within the gap are the same as one another. In certainembodiments, the gap includes one or more nucleoside having a sugarmoiety that differs from the sugar moiety of one or more othernucleosides of the gap. In certain embodiments, the sugar motifs of thetwo wings are the same as one another (symmetric gapmer). In certainembodiments, the sugar motif of the 5′-wing differs from the sugar motifof the 3′-wing (asymmetric gapmer).

In certain embodiments, the wings of a gapmer comprise 1-6 nucleosides.In certain embodiments, each nucleoside of each wing of a gapmercomprises a modified sugar moiety. In certain embodiments, at least onenucleoside of each wing of a gapmer comprises a modified sugar moiety.In certain embodiments, at least two nucleosides of each wing of agapmer comprises a modified sugar moiety. In certain embodiments, atleast three nucleosides of each wing of a gapmer comprises a modifiedsugar moiety. In certain embodiments, at least four nucleosides of eachwing of a gapmer comprises a modified sugar moiety. In certainembodiments, at least five nucleosides of each wing of a gapmercomprises a modified sugar moiety.

In certain embodiments, the gap of a gapmer comprises 7-12 nucleosides.In certain embodiments, each nucleoside of the gap of a gapmer comprisesa 2′-β-D-deoxyribosyl sugar moiety. In certain embodiments, at least onenucleoside of the gap of a gapmer comprises a modified sugar moiety.

In certain embodiments, the gapmer is a deoxy gapmer. In certainembodiments, the nucleosides on the gap side of each wing/gap junctioncomprise 2′- deoxyribosyl sugar moieties and the nucleosides on the wingsides of each wing/gap junction comprise modified sugar moieties. Incertain embodiments, each nucleoside of the gap comprises a2′-β-D-deoxyribosyl sugar moiety. In certain embodiments, eachnucleoside of each wing of a gapmer comprises a modified sugar moiety.In certain embodiments, exactly one nucleoside of the gap comprises amodified sugar moiety and each remaining nucleoside of the gap comprisesa 2′-β-D-deoxyribosyl sugar moiety.

In certain embodiments, modified oligonucleotides comprise or consist ofa portion having a fully modified sugar motif. In such embodiments, eachnucleoside of the fully modified portion of the modified oligonucleotidecomprises a modified sugar moiety. In certain embodiments, eachnucleoside of the entire modified oligonucleotide comprises a modifiedsugar moiety. In certain embodiments, modified oligonucleotides compriseor consist of a portion having a fully modified sugar motif, whereineach nucleoside within the fully modified portion comprises the samemodified sugar moiety, referred to herein as a uniformly modified sugarmotif. In certain embodiments, a fully modified oligonucleotide is auniformly modified oligonucleotide. In certain embodiments, eachnucleoside of a uniformly modified comprises the same 2′-modification.

Herein, the lengths (number of nucleosides) of the three regions of agapmer may be provided using the notation [# of nucleosides in the5′-wing]—[# of nucleosides in the gap]—[# of nucleosides in the3′-wing]. Thus, a 3-10-3 gapmer consists of 3 linked nucleosides in eachwing and 10 linked nucleosides in the gap. Where such nomenclature isfollowed by a specific modification, that modification is themodification in each sugar moiety of each wing and the gap nucleosidescomprise 2′-β-D-deoxyribosyl sugar moieties. Thus, a 5-10-5 MOE gapmerconsists of 5 linked 2′-MOE nucleosides in the 5′-wing, 10 linked 2′-β-D-deoxynucleosides in the gap, and 5 linked 2′-MOE nucleosides in the3′-wing. A 3-10-3 cEt gapmer consists of 3 linked cEt nucleosides in the5′-wing, 10 linked 2′- β-D-deoxynucleosides in the gap, and 3 linked cEtnucleosides in the 3′-wing. A 5-8-5 gapmer consists of 5 linkednucleosides comprising a modified sugar moiety in the 5′-wing, 8 linked2′-β-D-deoxynucleosides in the gap, and 5 linked nucleosides comprisinga modified sugar moiety in the 3′-wing. A 5-8-5 mixed gapmer has atleast two different modified sugar moieties in the 5′- and/or the3′-wing.

In certain embodiments, modified oligonucleotides are 5-10-5 MOEgapmers. In certain embodiments, modified oligonucleotides are 4-10-6MOE gapmers. In certain embodiments, modified oligonucleotides are6-10-4 MOE gapmers. In certain embodiments, modified oligonucleotidesare 5-8-5 MOE gapmers. In certain embodiments, modified oligonucleotidesare 6-8-4 MOE gapmers. In certain embodiments, modified oligonucleotidesare 4-8-6 MOE gapmers. In certain embodiments, modified oligonucleotidesare X-Y-Z MOE gapmers, wherein X and Z are independently selected from1, 2, 3, 4, 5, or 6 and Y is 7, 8, 9, 10, or 11.

2. Certain Nucleobase Motifs

In certain embodiments, oligonucleotides comprise modified and/orunmodified nucleobases arranged along the oligonucleotide or portionthereof in a defined pattern or motif. In certain embodiments, eachnucleobase is modified. In certain embodiments, none of the nucleobasesare modified. In certain embodiments, each purine or each pyrimidine ismodified. In certain embodiments, each adenine is modified. In certainembodiments, each guanine is modified. In certain embodiments, eachthymine is modified. In certain embodiments, each uracil is modified. Incertain embodiments, each cytosine is modified. In certain embodiments,some or all of the cytosine nucleobases in a modified oligonucleotideare 5-methyl cytosines. In certain embodiments, all of the cytosinenucleobases are 5-methyl cytosines and all of the other nucleobases ofthe modified oligonucleotide are unmodified nucleobases.

In certain embodiments, modified oligonucleotides comprise a block ofmodified nucleobases. In certain such embodiments, the block is at the3′-end of the oligonucleotide. In certain embodiments the block iswithin 3 nucleosides of the 3′-end of the oligonucleotide. In certainembodiments, the block is at the 5′-end of the oligonucleotide. Incertain embodiments the block is within 3 nucleosides of the 5′-end ofthe oligonucleotide.

In certain embodiments, oligonucleotides having a gapmer motif comprisea nucleoside comprising a modified nucleobase. In certain suchembodiments, one nucleoside comprising a modified nucleobase is in thecentral gap of an oligonucleotide having a gapmer motif. In certain suchembodiments, the sugar moiety of said nucleoside is a2′-β-D-deoxyribosyl sugar moiety. In certain embodiments, the modifiednucleobase is selected from: a 2-thiopyrimidine and a5-propynepyrimidine.

3. Certain Internucleoside Linkage Motifs

In certain embodiments, oligonucleotides comprise modified and/orunmodified internucleoside linkages arranged along the oligonucleotideor portion thereof in a defined pattern or motif. In certainembodiments, each internucleoside linking group is a phosphodiesterinternucleoside linkage (P(O₂)═O). In certain embodiments, eachinternucleoside linking group of a modified oligonucleotide is aphosphorothioate internucleoside linkage (P(O₂)═S). In certainembodiments, each internucleoside linkage of a modified oligonucleotideis independently selected from a phosphorothioate internucleosidelinkage and phosphodiester internucleoside linkage. In certainembodiments, each phosphorothioate internucleoside linkage isindependently selected from a stereorandom phosphorothioate, a (Sp)phosphorothioate, and a (Rp) phosphorothioate. In certain embodiments,the sugar motif of a modified oligonucleotide is a gapmer and theinternucleoside linkages within the gap are all modified. In certainsuch embodiments, some or all of the internucleoside linkages in thewings are unmodified phosphodiester internucleoside linkages. In certainembodiments, the terminal internucleoside linkages are modified. Incertain embodiments, the sugar motif of a modified oligonucleotide is agapmer, and the internucleoside linkage motif comprises at least onephosphodiester internucleoside linkage in at least one wing, wherein theat least one phosphodiester linkage is not a terminal internucleosidelinkage, and the remaining internucleoside linkages are phosphorothioateinternucleoside linkages. In certain such embodiments, all of thephosphorothioate linkages are stereorandom. In certain embodiments, allof the phosphorothioate linkages in the wings are (Sp)phosphorothioates, and the gap comprises at least one Sp, Sp, Rp motif.In certain embodiments, populations of modified oligonucleotides areenriched for modified oligonucleotides comprising such internucleosidelinkage motifs.

In certain embodiments, modified nucleotides have an internucleosidelinkage motif of soooossssssssssooss, wherein each “s” represents aphosphorothioate internucleoside linkage and each “o” represents aphosphodiester internucleoside linkage. In certain embodiments, modifiednucleotides have an internucleoside linkage motif of soooossssssssssoss,wherein each “s” represents a phosphorothioate internucleoside linkageand each “o” represents a phosphodiester internucleoside linkage. Incertain embodiments, modified nucleotides have an internucleosidelinkage motif of soooosssssssssoss, wherein each “s” represents aphosphorothioate internucleoside linkage and each “o” represents aphosphodiester internucleoside linkage. In certain embodiments, modifiednucleotides have an internucleoside linkage motif of sooosssssssssooss,wherein each “s” represents a phosphorothioate internucleoside linkageand each “o” represents a phosphodiester internucleoside linkage. Incertain embodiments, modified nucleotides have an internucleosidelinkage motif of sooossssssssssoooss, wherein each “s” represents aphosphorothioate internucleoside linkage and each “o” represents aphosphodiester internucleoside linkage. In certain embodiments, modifiednucleotides have an internucleoside linkage motif of soosssssssssoooss,wherein each “s” represents a phosphorothioate internucleoside linkageand each “o” represents a phosphodiester internucleoside linkage.

C. Certain Lengths

It is possible to increase or decrease the length of an oligonucleotidewithout eliminating activity. For example, in Woolf et al. (Proc. Natl.Acad. Sci. USA 89:7305-7309, 1992), a series of oligonucleotides 13-25nucleobases in length were tested for their ability to induce cleavageof a target RNA in an oocyte injection model. Oligonucleotides 25nucleobases in length with 8 or 11 mismatch bases near the ends of theoligonucleotides were able to direct specific cleavage of the targetRNA, albeit to a lesser extent than the oligonucleotides that containedno mismatches. Similarly, target specific cleavage was achieved using 13nucleobase oligonucleotides, including those with 1 or 3 mismatches.

In certain embodiments, oligonucleotides (including modifiedoligonucleotides) can have any of a variety of ranges of lengths. Incertain embodiments, oligonucleotides consist of X to Y linkednucleosides, where X represents the fewest number of nucleosides in therange and Y represents the largest number nucleosides in the range. Incertain such embodiments, X and Y are each independently selected from8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25,26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43,44, 45, 46, 47, 48, 49, and 50; provided that X≤Y. For example, incertain embodiments, oligonucleotides consist of 12 to 13, 12 to 14, 12to 15, 12 to 16, 12 to 17, 12 to 18, 12 to 19, 12 to 20, 12 to 21, 12 to22, 12 to 23, 12 to 24, 12 to 25, 12 to 26, 12 to 27, 12 to 28, 12 to29, 12 to 30, 13 to 14, 13 to 15, 13 to 16, 13 to 17, 13 to 18, 13 to19, 13 to 20, 13 to 21, 13 to 22, 13 to 23, 13 to 24, 13 to 25, 13 to26, 13 to 27, 13 to 28, 13 to 29, 13 to 30, 14 to 15, 14 to 16, 14 to17, 14 to 18, 14 to 19, 14 to 20, 14 to 21, 14 to 22, 14 to 23, 14 to24, 14 to 25, 14 to 26, 14 to 27, 14 to 28, 14 to 29, 14 to 30, 15 to16, 15 to 17, 15 to 18, 15 to 19, 15 to 20, 15 to 21, 15 to 22, 15 to23, 15 to 24, 15 to 25, 15 to 26, 15 to 27, 15 to 28, 15 to 29, 15 to30, 16 to 17, 16 to 18, 16 to 19, 16 to 20, 16 to 21, 16 to 22, 16 to23, 16 to 24, 16 to 25, 16 to 26, 16 to 27, 16 to 28, 16 to 29, 16 to30, 17 to 18, 17 to 19, 17 to 20, 17 to 21, 17 to 22, 17 to 23, 17 to24, 17 to 25, 17 to 26, 17 to 27, 17 to 28, 17 to 29, 17 to 30, 18 to19, 18 to 20, 18 to 21, 18 to 22, 18 to 23, 18 to 24, 18 to 25, 18 to26, 18 to 27, 18 to 28, 18 to 29, 18 to 30, 19 to 20, 19 to 21, 19 to22, 19 to 23, 19 to 24, 19 to 25, 19 to 26, 19 to 29, 19 to 28, 19 to29, 19 to 30, 20 to 21, 20 to 22, 20 to 23, 20 to 24,20 to 25, 20 to26,20 to 27, 20 to 28, 20 to 29, 20 to 30, 21 to 22, 21 to 23, 21 to 24,21 to 25, 21 to 26, 21 to 27, 21 to 28, 21 to 29, 21 to 30, 22 to 23, 22to 24, 22 to 25, 22 to 26, 22 to 27, 22 to 28, 22 to 29, 22 to 30, 23 to24, 23 to 25, 23 to 26, 23 to 27, 23 to 28, 23 to 29, 23 to 30, 24 to25, 24 to 26, 24 to 27, 24 to 28, 24 to 29, 24 to 30, 25 to 26, 25 to27, 25 to 28, 25 to 29, 25 to 30, 26 to 27, 26 to 28, 26 to 29, 26 to30, 27 to 28, 27 to 29, 27 to 30, 28 to 29, 28 to 30, or 29 to 30 linkednucleosides.

D. Certain Modified Oligonucleotides

In certain embodiments, the above modifications (sugar, nucleobase,internucleoside linkage) are incorporated into a modifiedoligonucleotide. In certain embodiments, modified oligonucleotides arecharacterized by their modification motifs and overall lengths. Incertain embodiments, such parameters are each independent of oneanother. Thus, unless otherwise indicated, each internucleoside linkageof an oligonucleotide having a gapmer sugar motif may be modified orunmodified and may or may not follow the gapmer modification pattern ofthe sugar modifications. For example, the internucleoside linkageswithin the wing regions of a sugar gapmer may be the same or differentfrom one another and may be the same or different from theinternucleoside linkages of the gap region of the sugar motif. Likewise,such sugar gapmer oligonucleotides may comprise one or more modifiednucleobase independent of the gapmer pattern of the sugar modifications.Unless otherwise indicated, all modifications are independent ofnucleobase sequence.

E. Certain Populations of Modified Oligonucleotides

Populations of modified oligonucleotides in which all of the modifiedoligonucleotides of the population have the same molecular formula canbe stereorandom populations or chirally enriched populations. All of thechiral centers of all of the modified oligonucleotides are stereorandomin a stereorandom population. In a chirally enriched population, atleast one particular chiral center is not stereorandom in the modifiedoligonucleotides of the population. In certain embodiments, the modifiedoligonucleotides of a chirally enriched population are enriched for β-Dribosyl sugar moieties, and all of the phosphorothioate internucleosidelinkages are stereorandom. In certain embodiments, the modifiedoligonucleotides of a chirally enriched population are enriched for bothβ-D ribosyl sugar moieties and at least one, particular phosphorothioateinternucleoside linkage in a particular stereochemical configuration.

F. Nucleobase Sequence

In certain embodiments, oligonucleotides (unmodified or modifiedoligonucleotides) are further described by their nucleobase sequence. Incertain embodiments oligonucleotides have a nucleobase sequence that iscomplementary to a second oligonucleotide or an identified referencenucleic acid, such as a target nucleic acid. In certain suchembodiments, a portion of an oligonucleotide has a nucleobase sequencethat is complementary to a second oligonucleotide or an identifiedreference nucleic acid, such as a target nucleic acid. In certainembodiments, the nucleobase sequence of a portion or entire length of anoligonucleotide is at least 50%, at least 60%, at least 70%, at least80%, at least 85%, at least 90%, at least 95%, or 100% complementary tothe second oligonucleotide or nucleic acid, such as a target nucleicacid.

II. Certain Oligommic Compounds

In certain embodiments, provided herein are oligomeric compounds, whichconsist of an oligonucleotide (modified or unmodified) and optionallyone or more conjugate groups and/or terminal groups. Conjugate groupsconsist of one or more conjugate moiety and a conjugate linker whichlinks the conjugate moiety to the oligonucleotide. Conjugate groups maybe attached to either or both ends of an oligonucleotide and/or at anyinternal position. In certain embodiments, conjugate groups are attachedto the 2¹-position of a nucleoside of a modified oligonucleotide. Incertain embodiments, conjugate groups that are attached to either orboth ends of an oligonucleotide are terminal groups. In certain suchembodiments, conjugate groups or terminal groups are attached at the 3′and/or 5′-end of oligonucleotides. In certain such embodiments,conjugate groups (or terminal groups) are attached at the 3′-end ofoligonucleotides. In certain embodiments, conjugate groups are attachednear the 3′-end of oligonucleotides. In certain embodiments, conjugategroups (or terminal groups) are attached at the 5′-end ofoligonucleotides. In certain embodiments, conjugate groups are attachednear the 5′-end of oligonucleotides.

Examples of terminal groups include but are not limited to conjugategroups, capping groups, phosphate moieties, protecting groups, modifiedor unmodified nucleosides, and two or more nucleosides that areindependently modified or unmodified.

A. Certain Conjugate Groups

In certain embodiments, oligonucleotides are covalently attached to oneor more conjugate groups. In certain embodiments, conjugate groupsmodify one or more properties of the attached oligonucleotide, includingbut not limited to pharmacodynamics, pharmacokinetics, stability,binding, absorption, tissue distribution, cellular distribution,cellular uptake, charge and clearance. In certain embodiments, conjugategroups impart a new property on the attached oligonucleotide, e.g.,fluorophores or reporter groups that enable detection of theoligonucleotide. Certain conjugate groups and conjugate moieties havebeen described previously, for example: cholesterol moiety (Letsinger etal., Proc. Natl. Acad. Sci. USA, 1989, 86, 6553-6556), cholic acid(Manoharan et al., Bioorg. Med. Chem. Lett., 1994, 4, 1053-1060), athioether, e.g., hexyl-S-tritylthiol (Manoharan et al., Ann. N.Y. Acad.Sci., 1992, 660, 306-309; Manoharan et al., Bioorg. Med. Chem. Lett.,1993, 3, 2765-2770), a thiocholesterol (Oberhauser et al., Nucl. AcidsRes., 1992, 20, 533-538), an aliphatic chain, e g., do-decan-diol orundecyl residues (Saison-Behmoaras et al., EMBO J., 1991, 10, 1111-1118;Kabanov et al., FEBS Lett., 1990, 259, 327-330; Svinarchuk et al.,Biochimie, 1993, 75, 49-54), a phospholipid, e.g.,di-hexadecyl-rac-glycerol or triethyl-ammonium1,2-di-O-hexadecyl-rac-glycero-3-H-phosphonate (Manoharan et al.,Tetrahedron Lett., 1995, 36, 3651-3654; Shea et al., Nucl. Acids Res.,1990, 18, 3777-3783), a polyamine or a polyethylene glycol chain(Manoharan et al., Nucleosides & Nucleotides, 1995, 14, 969-973), oradamantane acetic acid a palmityl moiety (Mishra et al., Biochim.Biophys. Acta, 1995, 1264, 229-237), an octadecylamine orhexylamino-cathonyl-oxycholesterol moiety (Crooke et al., J. Pharmacol.Exp. Ther., 1996, 277, 923-937), a tocopherol group (Nishina et al.,Molecular Therapy Nucleic Acids, 2015, 4, e220; and Nishina et al.,Molecular Therapy, 2008, 16, 734-740), or a GalNAc cluster (e.g.,WO2014/179620).

In certain embodiments, conjugate groups may be selected from any of aC22 alkyl, C20 alkyl, C16 alkyl, C10 alkyl, C21 alkyl, C19 alkyl, C18alkyl, C15 alkyl, C14 alkyl, C13 alkyl, C₁₂ alkyl, C11 alkyl, C9 alkyl,C8 alkyl, C7 alkyl, C6 alkyl, C5 alkyl, C22 alkenyl, C20 alkenyl, C16alkenyl, C10 alkenyl, C21 alkenyl, C19 alkenyl, C18 alkenyl, C15alkenyl, C14 alkenyl, C13 alkenyl, C₁₂ alkenyl, C11 alkenyl, C9 alkenyl,C8 alkenyl, C7 alkenyl, C6 alkenyl, or C5 alkenyl.

In certain embodiments, conjugate groups may be selected from any of C22alkyl, C20 alkyl, C16 alkyl, C10 alkyl, C21 alkyl, C19 alkyl, C18 alkyl,C15 alkyl, C14 alkyl, C13 alkyl, C₁₂ alkyl, C11 alkyl, C9 alkyl, C8alkyl, C7 alkyl, C6 alkyl, and C5 alkyl, where the alkyl chain has oneor more unsaturated bonds.

1. Conjugate Moieties

Conjugate moieties include, without limitation, intercalators, reportermolecules, polyamines, polyamides, peptides, carbohydrates, vitaminmoieties, polyethylene glycols, thioethers, polyethers, cholesterols,thiocholesterols, cholic acid moieties, folate, lipids, phospholipids,biotin, phenazine, phenanthridine, anthraquinone, adamantane, acridine,fluoresceins, rhodamines, coumarins, fluorophores, and dyes.

In certain embodiments, a conjugate moiety comprises an active drugsubstance, for example, aspirin, warfarin, phenylbutazone, ibuprofen,suprofen, fen-bufen, ketoprofen, (S)-(+)-pranoprofen, carprofen,dansylsarcosine, 2,3,5-triiodobenzoic acid, fingolimod, flufenamic acid,folinic acid, a benzothiadiazide, chlorothiazide, a diazepine,indo-methicin, a barbiturate, a cephalosporin, a sulfa drug, anantidiabetic, an antibacterial or an antibiotic.

2. Conjugate Linkers

Conjugate moieties are attached to oligonucleotides through conjugatelinkers. In certain oligomeric compounds, the conjugate linker is asingle chemical bond (i.e., the conjugate moiety is attached directly toan oligonucleotide through a single bond). In certain embodiments, theconjugate linker comprises a chain structure, such as a hydrocarbylchain, or an oligomer of repeating units such as ethylene glycol,nucleosides, or amino acid units.

In certain embodiments, a conjugate linker comprises one or more groupsselected from alkyl, amino, oxo, amide, disulfide, polyethylene glycol,ether, thioether, and hydroxylamino. In certain such embodiments, theconjugate linker comprises groups selected from alkyl, amino, oxo, amideand ether groups. In certain embodiments, the conjugate linker comprisesgroups selected from alkyl and amide groups. In certain embodiments, theconjugate linker comprises groups selected from alkyl and ether groups.In certain embodiments, the conjugate linker comprises at least onephosphorus moiety. In certain embodiments, the conjugate linkercomprises at least one phosphate group. In certain embodiments, theconjugate linker includes at least one neutral linking group.

In certain embodiments, conjugate linkers, including the conjugatelinkers described above, are bifunctional linking moieties, e.g., thoseknown in the art to be useful for attaching conjugate groups to parentcompounds, such as the oligonucleotides provided herein. In general, abifunctional linking moiety comprises at least two functional groups.One of the functional groups is selected to bind to a particular site ona parent compound and the other is selected to bind to a conjugategroup. Examples of functional groups used in a bifunctional linkingmoiety include but are not limited to electrophiles for reacting withnucleophilic groups and nucleophiles for reacting with electrophilicgroups. In certain embodiments, bifunctional linking moieties compriseone or more groups selected from amino, hydroxyl, carboxylic acid,thiol, alkyl, alkenyl, and alkynyl.

Examples of conjugate linkers include but are not limited topyrrolidine, 8-amino-3,6-dioxaoctanoic acid (ADO), succinimidyl4-(N-maleimidomethyl) cyclohexane-l-calboxylate (SMCC) and6-aminohexanoic acid (AHEX or AHA). Other conjugate linkers include butare not limited to substituted or unsubstituted C₁-C₁₀ alkyl,substituted or unsubstituted C₂-C₁₀ alkenyl or substituted orunsubstituted C₂-C₁₀ alkynyl, wherein a nonlimiting list of preferredsubstituent groups includes hydroxyl, amino, alkoxy, carboxy, benzyl,phenyl, nitro, thiol, thioalkoxy, halogen, alkyl, aryl, alkenyl andalkynyl.

In certain embodiments, conjugate linkers comprise 1-10linker-nucleosides. In certain embodiments, conjugate linkers comprise2-5 linker-nucleosides. In certain embodiments, conjugate linkerscomprise exactly 3 linker-nucleosides. In certain embodiments, conjugatelinkers comprise the TCA motif. In certain embodiments, suchlinker-nucleosides are modified nucleosides. In certain embodiments suchlinker-nucleosides comprise a modified sugar moiety. In certainembodiments, linker-nucleosides are unmodified. In certain embodiments,linker-nucleosides comprise an optionally protected heterocyclic baseselected from a purine, substituted purine, pyrimidine or substitutedpyrimidine. In certain embodiments, a cleavable moiety is a nucleosideselected from uracil, thymine, cytosine, 4-N-benzoylcytosine, 5-methylcytosine, 4-N-benzoyl-5-methyl cytosine, adenine, 6-N-benzoyladenine,guanine and 2-N-isobutyrylguanine. It is typically desirable forlinker-nucleosides to be cleaved from the oligomeric compound after itreaches a target tissue. Accordingly, linker-nucleosides are typicallylinked to one another and to the remainder of the oligomeric compoundthrough cleavable bonds. In certain embodiments, such cleavable bondsare phosphodiester bonds.

Herein, linker-nucleosides are not considered to be part of theoligonucleotide. Accordingly, in embodiments in which an oligomericcompound comprises an oligonucleotide consisting of a specified numberor range of linked nucleosides and/or a specified percentcomplementarity to a reference nucleic acid and the oligomeric compoundalso comprises a conjugate group comprising a conjugate linkercomprising linker-nucleosides, those linker-nucleosides are not countedtoward the length of the oligonucleotide and are not used in determiningthe percent complementarity of the oligonucleotide for the referencenucleic acid. For example, an oligomeric compound may comprise (1) amodified oligonucleotide consisting of 8-30 nucleosides and (2) aconjugate group comprising 1-10 linker-nucleosides that are contiguouswith the nucleosides of the modified oligonucleotide. The total numberof contiguous linked nucleosides in such an oligomeric compound is morethan 30. Alternatively, an oligomeric compound may comprise a modifiedoligonucleotide consisting of 8-30 nucleosides and no conjugate group.The total number of contiguous linked nucleosides in such an oligomericcompound is no more than 30. Unless otherwise indicated conjugatelinkers comprise no more than 10 linker-nucleosides. In certainembodiments, conjugate linkers comprise no more than 5linker-nucleosides. In certain embodiments, conjugate linkers compriseno more than 3 linker-nucleosides. In certain embodiments, conjugatelinkers comprise no more than 2 linker-nucleosides. In certainembodiments, conjugate linkers comprise no more than 1linker-nucleoside.

In certain embodiments, it is desirable for a conjugate group to becleaved from the oligonucleotide. For example, in certain circumstancesoligomeric compounds comprising a particular conjugate moiety are bettertaken up by a particular cell type, but once the oligomeric compound hasbeen taken up, it is desirable that the conjugate group be cleaved torelease the unconjugated or parent oligonucleotide. Thus, certainconjugate linkers may comprise one or more cleavable moieties. Incertain embodiments, a cleavable moiety is a cleavable bond. In certainembodiments, a cleavable moiety is a group of atoms comprising at leastone cleavable bond. In certain embodiments, a cleavable moiety comprisesa group of atoms having one, two, three, four, or more than fourcleavable bonds. In certain embodiments, a cleavable moiety isselectively cleaved inside a cell or subcellular compartment, such as alysosome. In certain embodiments, a cleavable moiety is selectivelycleaved by endogenous enzymes, such as nucleases.

In certain embodiments, a cleavable bond is selected from among: anamide, an ester, an ether, one or both esters of a phosphodiester, aphosphate ester, a carbamate, or a disulfide. In certain embodiments, acleavable bond is one or both of the esters of a phosphodiester. Incertain embodiments, a cleavable moiety comprises a phosphate orphosphodiester. In certain embodiments, the cleavable moiety is aphosphate or phosphodiester linkage between an oligonucleotide and aconjugate moiety or conjugate group.

In certain embodiments, a cleavable moiety comprises or consists of oneor more linker-nucleosides. In certain such embodiments, the one or morelinker-nucleosides are linked to one another and/or to the remainder ofthe oligomeric compound through cleavable bonds. In certain embodiments,such cleavable bonds are unmodified phosphodiester bonds. In certainembodiments, a cleavable moiety is 2′-deoxynucleoside that is attachedto either the 3′ or 5′-terminal nucleoside of an oligonucleotide by aphosphodiester internucleoside linkage and covalently attached to theremainder of the conjugate linker or conjugate moiety by a phosphate orphosphorothioate linkage. In certain such embodiments, the cleavablemoiety is 2′-deoxyadenosine.

3. Cell-Targeting Moieties

In certain embodiments, a conjugate group comprises a cell-targetingmoiety. In certain embodiments, a conjugate group has the generalformula:

wherein n is from Ito about 3, m is 0 when n is 1, m is 1 when n is 2 orgreater, j is 1 or 0, and k is 1 or 0.

In certain embodiments, n is 1, j is 1 and k is 0. In certainembodiments, n is 1, j is 0 and k is 1. In certain embodiments, n is 1,j is 1 and k is 1. In certain embodiments, n is 2, j is 1 and k is 0. Incertain embodiments, n is 2, j is 0 and k is 1. In certain embodiments,n is 2, j is 1 and k is 1. In certain embodiments, n is 3, j is 1 and kis 0. In certain embodiments, n is 3, j is 0 and k is 1. In certainembodiments, n is 3, j is 1 and k is 1.

In certain embodiments, conjugate groups comprise cell-targetingmoieties that have at least one tethered ligand In certain embodiments,cell-targeting moieties comprise two tethered ligands covalentlyattached to a branching group. In certain embodiments, cell-targetingmoieties comprise three tethered ligands covalently attached to abranching group.

B. Certain Terminal Groups

In certain embodiments, oligomeric compounds comprise one or moreterminal groups. In certain such embodiments, oligomeric compoundscomprise a stabilized 5′-phosphate. Stabilized 5′-phosphates include,but are not limited to 5′-phosphanates, including, but not limited to5′-vinylphosphonates. In certain embodiments, terminal groups compriseone or more abasic nucleosides and/or inverted nucleosides. In certainembodiments, terminal groups comprise one or more 2′-linked nucleosides.In certain such embodiments, the 2′-linked nucleoside is an abasicnucleoside.

III. Oligomeric Duplexes

In certain embodiments, oligomeric compounds described herein comprisean oligonucleotide, having a nucleobase sequence complementary to thatof a target nucleic acid. In certain embodiments, an oligomeric compoundis paired with a second oligomeric compound to form an oligomericduplex. Such oligomeric duplexes comprise a first oligomeric compoundhaving a portion complementary to a target nucleic acid and a secondoligomeric compound having a portion complementary to the firstoligomeric compound. In certain embodiments, the first oligomericcompound of an oligomeric duplex comprises or consists of (1) a modifiedor unmodified oligonucleotide and optionally a conjugate group and (2) asecond modified or unmodified oligonucleotide and optionally a conjugategroup. Either or both oligomeric compounds of an oligomeric duplex maycomprise a conjugate group. The oligonucleotides of each oligomericcompound of an oligomeric duplex may include non-complementaryoverhanging nucleosides.

IV. Antisense Activity

In certain embodiments, oligomeric compounds and oligomeric duplexes arecapable of hybridizing to a target nucleic acid, resulting in at leastone antisense activity; such oligomeric compounds and oligomericduplexes are antisense compounds. In certain embodiments, antisensecompounds have antisense activity when they reduce or inhibit the amountor activity of a target nucleic acid by 25% or more in the standard cellassay. In certain embodiments, antisense compounds selectively affectone or more target nucleic acid. Such antisense compounds comprise anucleobase sequence that hybridizes to one or more target nucleic acid,resulting in one or more desired antisense activity and does nothybridize to one or more non-target nucleic acid or does not hybridizeto one or more non-target nucleic acid in such a way that results insignificant undesired antisense activity.

In certain antisense activities, hybridization of an antisense compoundto a target nucleic acid results in recruitment of a protein thatcleaves the target nucleic acid. For example, certain antisensecompounds result in RNase H mediated cleavage of the target nucleicacid. RNase H is a cellular endonuclease that cleaves the RNA strand ofan RNA:DNA duplex. The DNA in such an RNA:DNA duplex need not beunmodified DNA. In certain embodiments, described herein are antisensecompounds that are sufficiently “DNA-like” to elicit RNase H activity.In certain embodiments, one or more non-DNA-like nucleoside in the gapof a gapmer is tolerated.

In certain antisense activities, an antisense compound or a portion ofan antisense compound is loaded into an RNA-induced silencing complex(RISC), ultimately resulting in cleavage of the target nucleic acid. Forexample, certain antisense compounds result in cleavage of the targetnucleic acid by Argonaute Antisense compounds that are loaded into RISCare RNAJ₁ compounds. RNAJ₁ compounds may be double-stranded (siRNA) orsingle-stranded (ssRNA).

In certain embodiments, hybridization of an antisense compound to atarget nucleic acid does not result in recruitment of a protein thatcleaves that target nucleic acid. In certain embodiments, hybridizationof the antisense compound to the target nucleic acid results inalteration of splicing of the target nucleic acid. In certainembodiments, hybridization of an antisense compound to a target nucleicacid results in inhibition of a binding interaction between the targetnucleic acid and a protein or other nucleic acid. In certainembodiments, hybridization of an antisense compound to a target nucleicacid results in alteration of translation of the target nucleic acid.

Antisense activities may be observed directly or indirectly. In certainembodiments, observation or detection of an antisense activity involvesobservation or detection of a change in an amount of a target nucleicacid or protein encoded by such target nucleic acid, a change in theratio of splice variants of a nucleic acid or protein and/or aphenotypic change in a cell or subject.

V. Certain Tar2et Nucleic Acids

In certain embodiments, oligomeric compounds comprise or consist of anoligonucleotide comprising a portion that is complementary to a targetnucleic acid. In certain embodiments, the target nucleic acid is anendogenous RNA molecule. In certain embodiments, the target nucleic acidencodes a protein. In certain such embodiments, the target nucleic acidis selected from: an endogenous antisense transcript that does notencode a protein (e.g., UBE3A-ATS), a mature mRNA, and a pre-mRNA,including intronic, exonic and untranslated regions. In certainembodiments, the target RNA is an antisense transcript. In certainembodiments, the target RNA is a mature mRNA. In certain embodiments,the target nucleic acid is a pre-mRNA. In certain such embodiments, thetarget region is entirely within an intron. In certain embodiments, thetarget region spans an intron/exon junction. In certain embodiments, thetarget region is at least 50% within an intron. In certain embodiments,the target nucleic acid is the RNA transcriptional product of aretrogene. In certain embodiments, the target nucleic acid is anon-coding RNA. In certain such embodiments, the target non-coding RNAis selected from: a long non-coding RNA, a short non-coding RNA, anintronic RNA molecule.

A. Complementarity/Mismatches to the Target Nucleic Acid

It is possible to introduce mismatch bases without eliminating activity.For example, Gautschi et al (J. Natl. Cancer Inst. 93:463-471, March2001) demonstrated the ability of an oligonucleotide having 100%complementarity to the bcl-2 mRNA and having 3 mismatches to the bcl-xLmRNA to reduce the expression of both bcl-2 and bcl-xL in vitro and invivo. Furthermore, this oligonucleotide demonstrated potent anti-tumoractivity in vivo. Maher and Dolnick (Nuc. Acid. Res. 16:3341-3358, 1988)tested a series of tandem 14 nucleobase oligonucleotides, and 28 and 42nucleobase oligonucleotides comprised of the sequence of two or three ofthe tandem oligonucleotides, respectively, for their ability to arresttranslation of human DHFR in a rabbit reticulocyte assay. Each of thethree 14 nucleobase oligonucleotides alone was able to inhibittranslation, albeit at a more modest level than the 28 or 42 nucleobaseoligonucleotides.

In certain embodiments, oligonucleotides are complementary to the targetnucleic acid over the entire length of the oligonucleotide. In certainembodiments, oligonucleotides are 99%, 95%, 90%, 85%, or 80%complementary to the target nucleic acid. In certain embodiments,oligonucleotides are at least 80% complementary to the target nucleicacid over the entire length of the oligonucleotide and comprise aportion that is 100% or fully complementary to a target nucleic acid. Incertain embodiments, the portion of full complementarity is 6, 7, 8, 9,10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or 24nucleobases in length.

In certain embodiments, oligonucleotides comprise one or more mismatchednucleobases relative to the target nucleic acid. In certain embodiments,antisense activity against the target is reduced by such mismatch, butactivity against a non-target is reduced by a greater amount. Thus, incertain embodiments selectivity of the oligonucleotide is improved. Incertain embodiments, the mismatch is specifically positioned within anoligonucleotide having a gapmer motif. In certain embodiments, themismatch is at position 1, 2, 3, 4, 5, 6, 7, or 8 from the 5′-end of thegap region. In certain embodiments, the mismatch is at position 9, 8, 7,6, 5, 4, 3, 2, 1 from the 3′-end of the gap region. In certainembodiments, the mismatch is at position 1, 2, 3, or 4 from the 5′-endof the wing region. In certain embodiments, the mismatch is at position4, 3, 2, or 1 from the 3′-end of the wing region.

B. UBE3A-ATS

In certain embodiments, oligomeric compounds comprise or consist of anoligonucleotide, or portion thereof, that is complementary to a targetnucleic acid, wherein the target nucleic acid is UBE3A-ATS. In certainembodiments, UBE3A-ATS nucleic acid has the sequence set forth in SEQ IDNO: 1 (GENBANK Accession No: NC_000015.10_TRUNC_24821647_25441028), SEQID NO: 2915 (Ensemble Gene ID ENSG00000224078), or SEQ ID NO: 2916 (thecDNA of Ensemble transcript ENST00000554726.1).

In certain embodiments, an oligomeric compound complementary to SEQ IDNO: 1, SEQ ID NO: 2915, or SEQ ID NO: 2916 is capable of reducingUBE3A-ATS in a cell. In certain embodiments, an oligomeric compoundcomplementary to SEQ ID NO: 1 is capable of increasing UBE3A RNA orprotein in a cell. In certain embodiments, an oligomeric compoundcomplementary to SEQ ID NO: 1, SEQ ID NO: 2915, or SEQ ID NO: 2916 iscapable of increasing paternal UBE3A RNA or protein in a cell. Incertain embodiments, the cell is in vifro. In certain embodiments, thecell is in a subject. In certain embodiments, the oligomeric compoundconsists of a modified oligonucleotide. In certain embodiments, anoligomeric compound complementary to SEQ ID NO: 1, SEQ ID NO: 2915, orSEQ ID NO: 2916 is capable of ameliorating one or more symptom orhallmark of a neurogenetic disorder when administered to a subject. Incertain embodiments, the neurogenetic disorder is AS. In certainembodiments, the symptoms or hallmarks are selected from developmentaldelays, ataxia, speech impairment, sleep problems, seizures, and EEGabnormalities.

In certain embodiments, an oligomeric compound complementary to SEQ IDNO: 1, SEQ ID NO: 2915, or SEQ ID NO: 2916 is capable of reducing thedetectable amount of UBE3A-ATS RNA in vifro by at least 10%, at least20%, at least 30%, at least 40%, at least 50%, at least 60%, at least70%, at least 80%, or at least 90%. In certain embodiments, anoligomeric compound complementary to SEQ ID NO: 1, SEQ ID NO: 2915, orSEQ ID NO: 2916 is capable of increasing the detectable amount of UBE3Aprotein in vitro by at least 10%, at least 20%, at least 30%, at least40%, at least 50%, at least 60%, at least 70%, at least 80%, or at least90%. In certain embodiments, an oligomeric compound complementary to SEQID NO: 1, SEQ ID NO: 2915, or SEQ ID NO: 2916 is capable of reducing thedetectable amount of UBE3A-ATS RNA in the CSF of a subject by at least10%, at least 20%, at least 30%, at least 40%, at least 50%, at least60%, at least 70%, at least 80%, or at least 90%. In certainembodiments, an oligomeric compound complementary to SEQ ID NO: 1, SEQID NO: 2915, or SEQ ID NO: 2916 is capable of increasing the detectableamount of UBE3A protein in the CSF of a subject by at least 10%, atleast 20%, at least 30%, at least 40%, at least 50%, at least 60%, atleast 70%, at least 80%, or at least 90%.

C. Certain Target Nucleic Acids in Certain Tissues

In certain embodiments, oligomeric compounds comprise or consist of anoligonucleotide comprising a portion that is complementary to a targetnucleic acid, wherein the target nucleic acid is expressed in apharmacologically relevant tissue. In certain embodiments, thepharmacologically relevant tissues are the cells and tissues thatcomprise the central nervous system. Such tissues include the cortex,hippocampus, and spinal cord.

VI. Certain Pharmaceutical Compositions

In certain embodiments, described herein are pharmaceutical compositionscomprising one or more oligomeric compounds. In certain embodiments, theone or more oligomeric compounds each consists of a modifiedoligonucleotide. In certain embodiments, the pharmaceutical compositioncomprises a pharmaceutically acceptable diluent or carrier. In certainembodiments, a pharmaceutical composition comprises or consists of asterile saline solution and one or more oligomeric compound. In certainembodiments, the sterile saline is pharmaceutical grade saline. Incertain embodiments, a pharmaceutical composition comprises or consistsof one or more oligomeric compound and sterile water. In certainembodiments, the sterile water is pharmaceutical grade water. In certainembodiments, a pharmaceutical composition comprises or consists of oneor more oligomeric compound and phosphate-buffered saline (PBS). Incertain embodiments, the sterile PBS is pharmaceutical grade PBS. Incertain embodiments, a pharmaceutical composition comprises or consistsof one or more oligomeric compound and artificial cerebrospinal fluid.In certain embodiments, the artificial cerebrospinal fluid ispharmaceutical grade.

In certain embodiments, a pharmaceutical composition comprises amodified oligonucleotide and artificial cerebrospinal fluid. In certainembodiments, a pharmaceutical composition consists of a modifiedoligonucleotide and artificial cerebrospinal fluid. In certainembodiments, a pharmaceutical composition consists essentially of amodified oligonucleotide and artificial cerebrospinal fluid. In certainembodiments, the artificial cerebrospinal fluid is pharmaceutical grade.

In certain embodiments, pharmaceutical compositions comprise one or moreoligomeric compound and one or more excipients. In certain embodiments,excipients are selected from water, salt solutions, alcohol,polyethylene glycols, gelatin, lactose, amylase, magnesium stearate,talc, silicic acid, viscous paraffin, hydroxymethylcellulose andpolyvinylpyrrolidone.

In certain embodiments, oligomeric compounds may be admixed withpharmaceutically acceptable active and/or inert substances for thepreparation of pharmaceutical compositions or formulations. Compositionsand methods for the formulation of pharmaceutical compositions depend ona number of criteria, including, but not limited to, route ofadministration, extent of disease, or dose to be administered.

In certain embodiments, pharmaceutical compositions comprising anoligomeric compound encompass any pharmaceutically acceptable salts ofthe oligomeric compound, esters of the oligomeric compound, or salts ofsuch esters. In certain embodiments, pharmaceutical compositionscomprising oligomeric compounds comprising one or more oligonucleotide,upon administration to a subject, including a human, are capable ofproviding (directly or indirectly) the biologically active metabolite orresidue thereof. Accordingly, for example, the disclosure is also drawnto pharmaceutically acceptable salts of oligomeric compounds, prodrugs,pharmaceutically acceptable salts of such prodrugs, and otherbioequivalents. Suitable pharmaceutically acceptable salts include, butare not limited to, sodium and potassium salts. In certain embodiments,prodrugs comprise one or more conjugate group attached to anoligonucleotide, wherein the conjugate group is cleaved by endogenousnucleases within the body.

Lipid moieties have been used in nucleic acid therapies in a variety ofmethods. In certain such methods, the nucleic acid, such as anoligomeric compound, is introduced into preformed liposomes orlipoplexes made of mixtures of cationic lipids and neutral lipids. Incertain methods, DNA complexes with mono- or poly-cationic lipids areformed without the presence of a neutral lipid. In certain embodiments,a lipid moiety is selected to increase distribution of a pharmaceuticalagent to a particular cell or tissue. In certain embodiments, a lipidmoiety is selected to increase distribution of a pharmaceutical agent tofat tissue. In certain embodiments, a lipid moiety is selected toincrease distribution of a pharmaceutical agent to muscle tissue.

In certain embodiments, pharmaceutical compositions comprise a deliverysystem. Examples of delivery systems include, but are not limited to,liposomes and emulsions. Certain delivery systems are useful forpreparing certain pharmaceutical compositions including those comprisinghydrophobic compounds. In certain embodiments, certain organic solventssuch as dimethylsulfoxide are used.

In certain embodiments, pharmaceutical compositions comprise one or moretissue-specific delivery molecules designed to deliver the one or morepharmaceutical agents of the present invention to specific tissues orcell types. For example, in certain embodiments, pharmaceuticalcompositions include liposomes coated with a tissue-specific antibody.

In certain embodiments, pharmaceutical compositions comprise aco-solvent system. Certain of such co-solvent systems comprise, forexample, benzyl alcohol, a nonpolar surfactant, a water-miscible organicpolymer, and an aqueous phase. In certain embodiments, such co-solventsystems are used for hydrophobic compounds. A non-limiting example ofsuch a co-solvent system is the VPD co-solvent system, which is asolution of absolute ethanol comprising 3% w/v benzyl alcohol, 8% w/v ofthe nonpolar surfactant Polysorbate 80™ and 65% w/v polyethylene glycol300. The proportions of such co-solvent systems may be variedconsiderably without significantly altering their solubility andtoxicity characteristics. Furthermore, the identity of co-solventcomponents may be varied: for example, other surfactants may be usedinstead of Polysorbate 80™; the fraction size of polyethylene glycol maybe varied; other biocompatible polymers may replace polyethylene glycol,e.g., polyvinyl pyrrolidone; and other sugars or polysaccharides maysubstitute for dextrose.

In certain embodiments, pharmaceutical compositions are prepared fororal administration. In certain embodiments, pharmaceutical compositionsare prepared for buccal administration. In certain embodiments, apharmaceutical composition is prepared for administration by injection(e.g., intravenous, subcutaneous, intramuscular, intrathecal (IT),intracerebroventricular (ICV), etc.). In certain of such embodiments, apharmaceutical composition comprises a carrier and is formulated inaqueous solution, such as water or physiologically compatible bufferssuch as Hanks's solution, Ringer's solution, or physiological salinebuffer. In certain embodiments, other ingredients are included (e.g.,ingredients that aid in solubility or serve as preservatives). Incertain embodiments, injectable suspensions are prepared usingappropriate liquid carriers, suspending agents and the like. Certainpharmaceutical compositions for injection are presented in unit dosageform, e.g., in ampoules or in multi-dose containers. Certainpharmaceutical compositions for injection are suspensions, solutions oremulsions in oily or aqueous vehicles, and may contain formulatoryagents such as suspending, stabilizing and/or dispersing agents. Certainsolvents suitable for use in pharmaceutical compositions for injectioninclude, but are not limited to, lipophilic solvents and fatty oils,such as sesame oil, synthetic fatty acid esters, such as ethyl oleate ortriglycerides, and liposomes.

Under certain conditions, certain compounds disclosed herein act asacids. Although such compounds may be drawn or described in protonated(free acid) form, or ionized and in association with a cation (salt)form, aqueous solutions of such compounds exist in equilibrium amongsuch forms. For example, a phosphate linkage of an oligonucleotide inaqueous solution exists in equilibrium among free acid, anion and saltforms. Unless otherwise indicated, compounds described herein areintended to include all such forms. Moreover, certain oligonucleotideshave several such linkages, each of which is in equilibrium. Thus,oligonucleotides in solution exist in an ensemble of forms at multiplepositions all at equilibrium. The term “oligonucleotide” is intended toinclude all such forms. Drawn structures necessarily depict a singleform. Nevertheless, unless otherwise indicated, such drawings arelikewise intended to include corresponding forms. Herein, a structuredepicting the free acid of a compound followed by the term “or a saltthereof” expressly includes all such forms that may be fully orpartially protonated/de-protonated/in association with a cation. Incertain instances, one or more specific cation is identified.

In certain embodiments, modified oligonucleotides or oligomericcompounds are in aqueous solution with sodium. In certain embodiments,modified oligonucleotides or oligomeric compounds are in aqueoussolution with potassium. In certain embodiments, modifiedoligonucleotides or oligomeric compounds are in PBS. In certainembodiments, modified oligonucleotides or oligomeric compounds are inwater. In certain such embodiments, the pH of the solution is adjustedwith NaOH and/or HCl to achieve a desired pH.

Herein, certain specific doses are described. A dose may be in the formof a dosage unit. For clarity, a dose (or dosage unit) of a modifiedoligonucleotide or an oligomeric compound in milligrams indicates themass of the free acid form of the modified oligonucleotide or oligomericcompound. As described above, in aqueous solution, the free acid is inequilibrium with anionic and salt forms. However, for the purpose ofcalculating dose, it is assumed that the modified oligonucleotide oroligomeric compound exists as a solvent-free, sodium-acetate free,anhydrous, free acid. For example, where a modified oligonucleotide oran oligomeric compound is in solution comprising sodium (e.g., saline),the modified oligonucleotide or oligomeric compound may be partially orfully de-protonated and in association with Na+ions. However, the massof the protons is nevertheless counted toward the weight of the dose,and the mass of the

Na+ions are not counted toward the weight of the dose. Thus, forexample, a dose, or dosage unit, of 100 mg of Compound No. 1263518equals the number of fully protonated molecules that weighs 100 mg. Thiswould be equivalent to 106 mg of solvent-free, sodium-acetate free,anhydrous sodiated Compound No. 1263518. When an oligomeric compoundcomprises a conjugate group, the mass of the conjugate group is includedin calculating the dose of such oligomeric compound. If the conjugategroup also has an acid, the conjugate group is likewise assumed to befully protonated for the purpose of calculating dose.

VII. Certain Compositions

1. Compound No. 1065645

In certain embodiments, Compound No. 1065645 is characterized as a5-10-5 MOE gapmer having a sequence (from 5′ to 3′) ofCATCATGATCTTGGTAAGGC (SEQ ID NO: 1949), wherein each of nucleosides 1-5and 16-20 (from 5′ to 3′) are 2′-MOE nucleosides and each of nucleosides6-15 are 2′-β-D-deoxynucleosides, wherein the internucleoside linkagesbetween nucleosides 2 to 3, 3 to 4, 4 to 5, 5 to 6, 16 to 17, and 17 to18 are phosphodiester internucleoside linkages and the internucleosidelinkages between nucleosides 1 to 2, 6 to 7, 7 to 8, 8 to 9, 9 to 10, 10to 11, 11 to 12, 12 to 13, 13 to 14, 14 to 15, 15 to 16, 18 to 19, and19 to 20 are phosphorothioate internucleoside linkages, and wherein eachcytosine is a 5-methyl cytosine.

In certain embodiments, Compound No. 1065645 is represented by thefollowing chemical notation: ^(m)C_(es)A_(eo)T_(eo)^(m)C_(eo)A_(eo)T_(ds)G_(ds)A_(ds)T_(ds)^(m)C_(ds)T_(ds)T_(ds)G_(ds)G_(ds)T_(ds)A_(eo)A_(eo)G_(es)G_(es)^(m)C_(e) (SEQ ID NO: 1949), wherein:

A=an adenine nucleobase,

mC=a 5-methyl cytosine nucleobase,

G=a guanine nucleobase,

T=a thymine nucleobase,

e=a 2′-MOE sugar moiety,

d=a 2′-β-D-deoxyribosyl sugar moiety,

s=a phosphorothioate internucleoside linkage, and

o=a phosphodiester internucleoside linkage.

In certain embodiments, Compound No. 1065645 is represented by thefollowing chemical structure:

In certain embodiments, the sodium salt of Compound No. 1065645 isrepresented by the following chemical structure:

2. Compound No. 1263517

In certain embodiments, Compound No. 1263517 is characterized as a6-10-4 MOE gapmer having a sequence (from 5′ to 3′) ofTCACCATTTTGACCTTCTTA (SEQ ID NO: 2751), wherein each of nucleosides 1-6and 17-20 (from 5′ to 3′) are 2′-MOE nucleosides and each of nucleosides7-16 are 2′-β-D-deoxynucleosides, wherein the internucleoside linkagesbetween nucleosides 2 to 3, 3 to 4, 4 to 5, 5 to 6, 6 to 7, and 17 to 18are phosphodiester internucleoside linkages and the internucleosidelinkages between nucleosides 1 to 2, 7 to 8, 8 to 9, 9 to 10, 10 to 11,11 to 12, 12 to 13, 13 to 14, 14 to 15, 15 to 16, 16 to 17, 18 to 19,and 19 to 20 are phosphorothioate internucleoside linkages, and whereineach cytosine is a 5-methyl cytosine.

In certain embodiments, Compound No. 1263517 is represented by thefollowing chemical notation: T_(es) ^(m)C_(eo)A_(eo) ^(m)C_(eo)^(m)C_(eo)A_(eo)T_(ds)T_(ds)T_(ds)T_(ds)G_(ds)A_(ds) ^(m)C_(ds)^(m)C_(ds)T_(ds)T_(ds) ^(m)C_(eo)T_(es)T_(es)A_(e)(SEQ ID NO: 2751),wherein:

A=an adenine nucleobase,

mC=a 5-methyl cytosine nucleobase,

G=a guanine nucleobase,

T=a thymine nucleobase,

e=a 2′-MOE sugar moiety,

d=a 2′-β-D-deoxyribosyl sugar moiety,

s=a phosphorothioate internucleoside linkage, and

o=a phosphodiester internucleoside linkage.

In certain embodiments, Compound No. 1263517 is represented by thefollowing chemical structure:

In certain embodiments, the sodium salt of Compound No. 1263517 isrepresented by the following chemical structure:

3. Compound No. 1263518

In certain embodiments, Compound No. 1263518 is characterized as a6-10-4 MOE gapmer having a sequence (from 5′ to 3′) ofTTCACCATTTTGACCTTCTT (SEQ ID NO: 2752), wherein each of nucleosides 1-6and 17-20 (from 5′ to 3′) are 2′-MOE nucleosides and each of nucleosides7-16 are 2′-β-D-deoxynucleosides, wherein the internucleoside linkagesbetween nucleosides 2 to 3, 3 to 4, 4 to 5, 5 to 6, 6 to 7, and 17 to 18are phosphodiester internucleoside linkages and the internucleosidelinkages between nucleosides 1 to 2, 7 to 8, 8 to 9, 9 to 10, 10 to 11,11 to 12, 12 to 13, 13 to 14, 14 to 15, 15 to 16, 16 to 17, 18 to 19,and 19 to 20 are phosphorothioate internucleoside linkages, and whereineach cytosine is a 5-methyl cytosine.

In certain embodiments, Compound No. 1263518 is represented by thefollowing chemical notation: T_(es)T_(eo) ^(m)C_(eo)A_(eo) ^(m)C_(eo)^(m)C_(eo)A_(ds)T_(ds)T_(ds)T_(ds)T_(ds)G_(ds)A_(ds) ^(m)C_(ds)^(m)C_(ds)T_(ds)T_(eo) ^(m)C_(es)T_(es)T_(e)(SEQ ID NO: 2752), wherein:

A=an adenine nucleobase,

mC=a 5-methyl cytosine nucleobase,

G=a guanine nucleobase,

T=a thymine nucleobase,

e=a 2′-MOE sugar moiety,

d=a 2′-β-D-deoxyribosyl sugar moiety,

s=a phosphorothioate internucleoside linkage, and

o=a phosphodiester internucleoside linkage.

In certain embodiments, Comnound No 1263518 is renresented by thefollowing chemical structure:

In certain embodiments, the sodium salt of Compound No. 1263518 isrepresented by the following chemical structure:

4. Compound No. 1263533

In certain embodiments, Compound No. 1263533 is characterized as a6-10-4 MOE gapmer having a sequence (from 5′ to 3′) ofGCATACCCAGGGTAGGATTC (SEQ ID NO: 765), wherein each of nucleosides 1-6and 17-20 (from 5′ to 3′) are 2′-MOE nucleosides and each of nucleosides7-16 are 2′-β-D-deoxynucleosides, wherein the internucleoside linkagesbetween nucleosides 2 to 3, 3 to 4, 4 to 5, 5 to 6, 6 to 7, and 17 to 18are phosphodiester internucleoside linkages and the internucleosidelinkages between nucleosides 1 to 2, 7 to 8, 8 to 9, 9 to 10, 10 to 11,11 to 12, 12 to 13, 13 to 14, 14 to 15, 15 to 16, 16 to 17, 18 to 19,and 19 to 20 are phosphorothioate internucleoside linkages, and whereineach cytosine is a 5-methyl cytosine.

In certain embodiments, Compound No. 1263533 is represented by thefollowing chemical notation: G_(es) ^(m)C_(eo)A_(eo)T_(eo)A_(eo)^(m)C_(eo) ^(m)C_(ds)^(m)C_(ds)A_(ds)G_(ds)G_(ds)G_(ds)T_(ds)A_(ds)G_(ds)G_(ds)A_(eo)T_(es)T_(es)^(m)C_(e) (SEQ ID NO: 765), wherein:

A=an adenine nucleobase,

mC=a 5-methyl cytosine nucleobase,

G=a guanine nucleobase,

T=a thymine nucleobase,

e=a 2′-MOE sugar moiety,

d=a 2′-β-D-deoxyribosyl sugar moiety,

s=a phosphorothioate internucleoside linkage, and

o=a phosphodiester internucleoside linkage.

In certain embodiments, Compound No. 1263533 is represented by thefollowing chemical structure:

In certain embodiments, the sodium salt of Compound No. 1263533 isrepresented by the chemical structure:

5. Compound No. 1273039

In certain embodiments, Compound No. 1273039 is characterized as a 4-8-6MOE gapmer having a sequence (from 5′ to 3′) of ACGCAATGTATCAGGCAA (SEQID NO: 2866), wherein each of nucleosides 1-4 and 13-18 (from 5′ to 3′)are 2′-MOE nucleosides and each of nucleosides 5-12 are2′-β-D-deoxynucleosides, wherein the internucleoside linkages betweennucleosides 2 to 3, 3 to 4, 13 to 14, 14 to 15, and 15 to 16 arephosphodiester internucleoside linkages and the internucleoside linkagesbetween nucleosides 1 to 2, 4 to 5, 5 to 6, 6 to 7, 7 to 8, 8 to 9, 9 to10, 10 to 11, 11 to 12, 12 to 13, 16 to 17, and 17 to 18 arephosphorothioate internucleoside linkages, and wherein each cytosine isa 5-methyl cytosine.

In certain embodiments, Compound No. 1273039 is represented by thefollowing chemical notation: A_(es) ^(m)C_(eo)G_(eo)^(m)C_(es)A_(ds)A_(ds)T_(ds)G_(ds)T_(ds)A_(ds)T_(ds)^(m)C_(ds)A_(eo)G_(eo)G_(eo) ^(m)C_(es)A_(es)A_(e) (SEQ ID NO: 2866),wherein:

A=an adenine nucleobase,

mC=a 5-methyl cytosine nucleobase,

G=a guanine nucleobase,

T=a thymine nucleobase,

e=a 2′-MOE sugar moiety,

d=a 2′-β-D-deoxyribosyl sugar moiety,

s=a phosphorothioate internucleoside linkage, and

o=a phosphodiester internucleoside linkage.

In certain embodiments, Compound No. 1273039 is represented by thefollowing chemical structure:

In certain embodiments, the sodium salt of Compound No. 1273039 isrepresented by the following chemical structure:

6. Compound No. 1273062

In certain embodiments, Compound No. 1273062 is characterized as a5-10-5 MOE gapmer having a sequence (from 5′ to 3′) ofACCATTTTGACCTTCTTAGC (SEQ ID NO: 2873), wherein each of nucleosides 1-5and 16-20 (from 5′ to 3′) are 2′-MOE nucleosides and each of nucleosides6-15 are 2′-β-D-deoxynucleosides, wherein the internucleoside linkagesbetween nucleosides 2 to 3, 3 to 4, 4 to 5, 5 to 6, 16 to 17, and 17 to18 are phosphodiester internucleoside linkages and the internucleosidelinkages between nucleosides 1 to 2, 6 to 7, 7 to 8, 8 to 9, 9 to 10, 10to 11, 11 to 12, 12 to 13, 13 to 14, 14 to 15, 15 to 16, 18 to 19, and19 to 20 are phosphorothioate internucleoside linkages, and wherein eachcytosine is a 5-methyl cytosine.

In certain embodiments, Compound No. 1273062 is represented by thefollowing chemical notation: A_(es) ^(m)C_(eo)^(m)C_(eo)A_(eo)T_(eo)T_(ds)T_(ds)T_(ds)G_(ds)A_(ds) ^(m)C_(ds)^(m)C_(ds)T_(ds)T_(ds) ^(m)C_(ds)T_(eo)T_(eo)A_(es)G_(es) ^(m)C_(e) (SEQID NO: 2873), wherein:

A=an adenine nucleobase,

mC=a 5-methyl cytosine nucleobase,

G=a guanine nucleobase,

T=a thymine nucleobase,

e=a 2′-MOE sugar moiety,

d=a 2′-β-D-deoxyribosyl sugar moiety,

s=a phosphorothioate internucleoside linkage, and

o=a phosphodiester internucleoside linkage.

In certain embodiments, Compound No. 1273062 is represented by thefollowing chemical structure:

In certain embodiments, the sodium salt of Compound No. 1273062 isrepresented by the following chemical structure:

VIII. Certain Comparator Compounds

In certain embodiments, Compound No. 1219022, a surrogate of which(Compound #586_9) is provided in WO2017/081223 (incorporated herein byreference), was used as a comparator compound. Compound No. 1219022 is amixed LNA/DNA oligonucleotide having a sugar motif of (from 5′ to 3′)llddldldddddddddllll, wherein each “1” represents an LNA sugar moietyand each “d” represents a 2′-β-D-deoxyribosyl sugar moiety; having asequence of (from 5′ to 3′) AAATTATTTATACACCATCA (SEQ ID NO: 2905),wherein each “C” is a 5-methyl cytosine; and wherein eachinternucleoside linkage is a phosphorothioate internucleoside linkage.Compound #586_9 has cytosines at positions 13, 15, and 16 whereasCompound No. 1219022 5-methyl cytosines at those positions. According toWan and Seth, “Nntroduction of the 5-methyl group on cytosine reducesthe immunostimulatory profile of certain DNA oligonucleotides and alsoenhances nuclease stability” (J. Med. Chem. 2016, 59, 9645-9667).

In certain embodiments, Compound No. 1219023, a surrogate of which(Compound #572_7) is provided in WO2017/081223 (incorporated herein byreference), was used as a comparator compound. Compound No. 1219023 is amixed LNA/DNA oligonucleotide having a sugar motif of (from 5′ to 3′)lllddldddddddddlll, wherein each “1” represents an LNA sugar moiety andeach “d” represents a 2′-ß-D-deoxyribosyl sugar moiety; having asequence of (from 5′ to 3′) TTTATCAATATCTTCTCA (SEQ ID NO: 2906),wherein each “C” is a 5-methyl cytosine; and wherein eachinternucleoside linkage is a phosphorothioate internucleoside linkage.Compound #572_7 has cytosines at positions 12 and 15 whereas CompoundNo. 1219023 has 5-methyl cytosines at those positions.

In certain embodiments, Compound No. 1219024, a surrogate of which(Compound #591_1) is provided in WO2017/081223 (incorporated herein byreference), was used as a comparator compound. Compound No. 1219024 is amixed LNA/DNA oligonucleotide having a sugar motif of (from 5′ to 3′)ldldlddddddddddddll, wherein each “1” represents an LNA sugar moiety andeach “d” represents a 2′-ß-D-deoxyribosyl sugar moiety; having asequence of (from 5′ to 3′) GCACATTCTTTCTATACCT (SEQ ID NO: 2907),wherein each “C” is a 5-methyl cytosine; and wherein eachinternucleoside linkage is a phosphorothioate internucleoside linkage.Compound #591_1 has cytosines at positions 2, 4, 8, 12 and 17 whereasCompound No. 1219024 has 5-methyl cytosines at those positions.

In certain embodiments, Compound No. 1219025, a surrogate of which(Compound #169_52) is provided in WO2017/081223 (incorporated herein byreference), was used as a comparator compound. Compound No. 1219025 is amixed LNA/DNA oligonucleotide having a sugar motif of (from 5′ to 3′)lldllddddddddddll, wherein each “1” represents an LNA sugar moiety andeach “d” represents a 2′-ß-D-deoxyribosyl sugar moiety; having asequence of (from 5′ to 3′) TTATAGCCATTCTATCT (SEQ ID NO: 2908), whereineach “C” is a 5-methyl cytosine; and wherein each internucleosidelinkage is a phosphorothioate internucleoside linkage. Compound #169_52has cytosines at positions 7, 8, and 12 whereas Compound No. 1219025 has5-methyl cytosines at those positions.

In certain embodiments, Compound No. 1219026, a surrogate of which(Compound #624_5) is provided in WO2017/081223 (incorporated herein byreference), was used as a comparator compound. Compound No. 1219026 is amixed LNA/DNA oligonucleotide having a sugar motif of (from 5′ to 3′)ldlllddddddddllll, wherein each “1” represents an LNA sugar moiety andeach “d” represents a 2′-ß-D-deoxyribosyl sugar moiety; having asequence of (from 5′ to 3′) CTCAAAGATCATTCTCA (SEQ ID NO: 2909), whereineach “C” is a 5-methyl cytosine; and wherein each internucleosidelinkage is a phosphorothioate internucleoside linkage. Compound # 624_5has a cytosine at position 10 whereas Compound No. 1219026 has a5-methyl cytosine at that position.

In certain embodiments, Compound No. 1219027, a surrogate of which(Compound #626_8) is provided in WO2017/081223 (incorporated herein byreference), was used as a comparator compound. Compound No. 1219027 is amixed LNA/DNA oligonucleotide having a sugar motif of (from 5′ to 3′)ldldldldddddddddldll, wherein each “1” represents an LNA sugar moietyand each “d” represents a 2′-ß-D-deoxyribosyl sugar moiety; having asequence of (from 5′ to 3′) TTACACTTAATTATACTTCC (SEQ ID NO: 2910),wherein each “C” is a 5-methyl cytosine; and wherein eachinternucleoside linkage is a phosphorothioate internucleoside linkage.Compound #626_8 has cytosines at positions 4, 6, and 16 whereas CompoundNo. 1219027 has 5-methyl cytosines at those positions.

In certain embodiments, Compound No. 1219028, a surrogate of which(Compound #639_5) is provided in WO2017/081223 (incorporated herein byreference), was used as a comparator compound. Compound No. 1219028 is amixed LNA/DNA oligonucleotide having a sugar motif of (from 5′ to 3′)ldddddddddddlldlll, wherein each “1” represents an LNA sugar moiety andeach “d” represents a 2′-ß-D-deoxyribosyl sugar moiety; having asequence of (from 5′ to 3′) GTTTCCATCTACTATTAA (SEQ ID NO: 2911),wherein each “C” is a 5-methyl cytosine; and wherein eachinternucleoside linkage is a phosphorothioate internucleoside linkage.Compound #639_5 has cytosines at positions 5, 6, 9 and 12 whereasCompound No. 1219028 has 5-methyl cytosines at those positions.

In certain embodiments, Compound No. 1219029, a surrogate of which(Compound #642_12) is provided in WO2017/081223 (incorporated herein byreference), was used as a comparator compound. Compound No. 1219029 is amixed LNA/DNA oligonucleotide having a sugar motif of (from 5′ to 3′)llddlddddddddddll, wherein each “1” represents an LNA sugar moiety andeach “d” represents a 2′-ß-D-deoxyribosyl sugar moiety; having asequence of (from 5′ to 3′) CTGTATACACCATCCCA (SEQ ID NO: 2912), whereineach “C” is a 5-methyl cytosine; and wherein each internucleosidelinkage is a phosphorothioate internucleoside linkage. Compound #642_12has cytosines at positions 8, 10, 11, 14 and 15 whereas Compound No.1219029 has 5-methyl cytosines at those positions.

In certain embodiments, Compound No. 1219030, a surrogate of which(Compound #304_6) is provided in WO2017/081223 (incorporated herein byreference), was used as a comparator compound. Compound No. 1219030 is amixed LNA/DNA oligonucleotide having a sugar motif of (from 5′ to 3′)lddllddddddddllll, wherein each “1” represents an LNA sugar moiety andeach “d” represents a 2′-ß-D-deoxyribosyl sugar moiety, having asequence of (from 5′ to 3′) AGTTCTACTATACTTTC (SEQ ID NO: 2913), whereineach “C” is a 5-methyl cytosine; and wherein each internucleosidelinkage is a phosphorothioate internucleoside linkage. Compound #304_6has cytosines at positions 8 and 13 whereas Compound No. 1219030 has5-methyl cytosines at those positions.

In certain embodiments, Compound No. 1219031, a surrogate of which(Compound #573_8) is provided in WO2017/081223 (incorporated herein byreference), was used as a comparator compound. Compound No. 1219031 is amixed LNA/DNA oligonucleotide having a sugar motif of (from 5′ to 3′)llddldldddddddddddll, wherein each “1” represents an LNA sugar moietyand each “d” represents a 2′-ß-D-deoxyribosyl sugar moiety; having asequence of (from 5′ to 3′) TATACCTTTCTTTAACCCTT (SEQ ID NO: 2914),wherein each “C” is a 5-methyl cytosine; and wherein eachinternucleoside linkage is a phosphorothioate internucleoside linkage.Compound #573_8 has cytosines at positions 6, 10, 16, 17, and 18 whereasCompound No. 1219031 has cytosines at those positions.

Compound Nos. 1219022-1219031 (relating to Compound Nos. #586_9, #572_7,#591_1, #169_52, #624_5, #626_8, #639_5 #642_12, #304_6, #573_8,respectively) were selected as comparator compounds from Example 7 ofWO2017/081223, which provides a subset of active compounds “selected forpotency and efficacy testing.”

In certain embodiments, compounds described herein are superior relativeto compounds described in WO2017/081223 because they demonstrate one ormore improved properties, such as in vivo tolerability.

For example, as described herein, certain compounds Compound No.1263517, Compound No. 1263518, Compound No. 1263533, Compound No.1273039, and Compound No. 1273062 achieved 3-hour FOB scores in mice of1.0 (Table 88), 0.0 (Table 88), 1.0 (Table 88), 1.0 (Table 96), and 0.0(Table 96), respectively, whereas each of comparator compounds CompoundNo. 1219022, Compound No. 1219024, Compound No. 1219025, Compound No.1219028, Compound No. 1219029, Compound No. 1219030, and Compound No.1219031 achieved 3-hour FOB scores in mice of 7.0, 5.3, 4.0, 6.0, 6.3,5.0, and 5.3 (Table 119), respectively. Therefore, certain compoundsdescribed herein are more tolerable than comparator compounds CompoundNo. 1219022, Compound No. 1219024, Compound No. 1219025, Compound No.1219028, Compound No. 1219029, Compound No. 1219030, and Compound No.1219031 in this assay.

For example, as described herein, certain compounds Compound No.1065645, Compound No. 1263517, Compound No. 1263518, Compound No.1263533, Compound No. 1273039, and Compound No. 1273062 each achieved2-week FOB scores in mice of 0.0 (Example 12, Table 112), whereasCompound No. 1219023, Compound No. 1219024, Compound No. 1219025,Compound No. 1219026, Compound No. 1219028, Compound No. 1219029,Compound No. 1219030, and Compound No. 1219031 achieved 2-week delayedFOB scores in mice of 3.5, 6.5, 6.0, 6.0, 6.0, 6.0, 6.0, 5.0, and 6.0,respectively (Table 119). Therefore, certain compounds described hereinare more tolerable than comparator compounds Compound No. 1219023 andCompound No. 1219026 in this assay.

For example, as described herein, certain compounds Compound No.1065645, Compound No. 1263517, Compound No. 1263518, Compound No.1263533, Compound No. 1273039, and Compound No. 1273062 do not cause asignificant difference in body weight as compared to PBS-treated rats inan 8-week study, whereas treatment with Compound No. 1219027 leads to agreater than 10% weight loss compared to PBS-treated rats (p-value<0.05, Table 120). Therefore, certain compounds described herein aremore tolerable than comparator Compound No. 1219027 in this assay.

IX. Certain Hotspot Regions

In certain embodiments, nucleobases in the ranges specified belowcomprise a hotspot region of UBE3A-ATS. In certain embodiments, modifiedoligonucleotides that are complementary to a hotspot region of UBE3A-ATSachieve an average of more than 50% reduction of UBE3A-ATS RNA in vitroin the standard cell assay.

1. Nucleobases 461,413-461,487 of SEQ ID NO: 1

In certain embodiments, nucleobases 461,413-461,487 of SEQ ID NO: 1comprise a hotspot region. In certain embodiments, modifiedoligonucleotides are complementary to a portion of nucleobases461,413-461,487 of SEQ ID NO: 1. In certain embodiments, modifiedoligonucleotides are 20 nucleobases in length. In certain embodiments,modified oligonucleotides are 18 nucleobases in length. In certainembodiments, modified oligonucleotides are gapmers. In certainembodiments, the gapmers are MOE gapmers. In certain embodiments, theinternucleoside linkages of the modified oligonucleotides arephosphorothioate internucleoside linkages and phosphodiesterinternucleoside linkages. In certain embodiments, the phosphodiester(“o”) and phosphorothioate (“s”) internucleoside linkages are arrangedin order from 5′ to 3′: soooossssssssssooss, sooooossssssssssoss,soooosssssssssoss, sooosssssssssooss, sooossssssssssoooss, orsoosssssssssoooss.

The nucleobase sequences of SEQ ID Nos: 1053, 1329, 1501, 1576, 1873,1949, 2025, 2096, 2245, 2512,2591,2680-2682, and 2844 are complementaryto a portion of nucleobases 461,413-461,487 of SEQ ID NO: 1.

The nucleobase sequenc of Compound Nos: 749901-749904, 1065641-1065646,1165562-1165563, 1165857-1165858, and 1273001 are complementary to aportion of nucleobases 461,413-461,487 of SEQ ID NO: 1.

In certain embodiments, modified oligonucleotides complementary to aportion of nucleobases 461,413-461,487 of SEQ ID NO: 1 achieve at least36% reduction of UBE3A-ATS RNA in vitro in the standard cell assay. Incertain embodiments, modified oligonucleotides complementary to aportion of nucleobases 461,413-461,487 of SEQ ID NO: 1 achieve anaverage of 60% reduction of UBE3A-ATS RNA in vitro in the standard cellassay.

2. Nucleobases 468,968-469,013 of SEQ ID NO: 1

In certain embodiments, nucleobases 468,968-469,013 of SEQ ID NO: 1comprise a hotspot region. In certain embodiments, modifiedoligonucleotides are complementary to a portion of nucleobases468,968-469,013 of SEQ ID NO: 1. In certain embodiments, modifiedoligonucleotides are 20 nucleobases in length. In certain embodiments,modified oligonucleotides are 18 nucleobases in length. In certainembodiments, modified oligonucleotides are gapmers.

In certain embodiments, the gapmers are MOE gapmers. In certainembodiments, the internucleoside linkages of the modifiedoligonucleotides are phosphorothioate internucleoside linkages andphosphodiester internucleoside linkages. In certain embodiments, thephosphodiester (“o”) and phosphorothioate (“s”) internucleoside linkagesare arranged in order from 5′ to 3′: soooossssssssssooss,sooooossssssssssoss, soooosssssssssoss, sooosssssssssooss,sooossssssssssoooss, or soosssssssssoooss.

The nucleobase sequences of SEQ ID Nos: 376, 377, 2751-2756, 2773-2776,2872, 2873, 2876-2878 are complementary to a portion of nucleobases468,968-469,013 of SEQ ID NO: 1.

The nucleobase sequence of Compound Nos: 750031-750032, 1263408-1263411,1263426, 1263441, 1263460-1263465, 1263486-1263492, 1263517-1263523,1273061, 1273062, and 1273065-1273067 are complementary to a portion ofnucleobases 468,968-469,013 of SEQ ID NO: 1.

In certain embodiments, modified oligonucleotides complementary to aportion of nucleobases 468,968-469,013 of SEQ ID NO: 1 achieve at least75% reduction of UBE3A-ATS RNA in vitro in the standard cell assay. Incertain embodiments, modified oligonucleotides complementary to aportion of nucleobases 468,968-469,013 of SEQ ID NO: 1 achieve anaverage of 78% reduction of UBE3A-ATS RNA in vitro in the standard cellassay. In certain embodiments, modified oligonculeotides complementaryto a portion of nucleobases 468,968-469,013 of SEQ ID NO: 1 achieve anaverage of 410% upregulation of UBE3A-ATS RNA in vitro at 6.7 μM in cellculture.

3. Nucleobases 483,965-484,003 of SEQ ID NO: 1

In certain embodiments, nucleobases 483,965-484,003 of SEQ ID NO: 1comprise a hotspot region. In certain embodiments, modifiedoligonucleotides are complementary to a portion of 483,965-484,003 ofSEQ ID NO: 1. In certain embodiments, modified oligonucleotides are 20nucleobases in length. In certain embodiments, modified oligonucleotidesare 18 nucleobases in length. In certain embodiments, modifiedoligonucleotides are gapmers. In certain embodiments, the gapmers areMOE gapmers. In certain embodiments, the internucleoside linkages of themodified oligonucleotides are phosphorothioate internucleoside linkagesand phosphodiester internucleoside linkages. In certain embodiments, thephosphodiester (“o”) and phosphorothioate (“s”) internucleoside linkagesare arranged in order from 5′ to 3′: soooossssssssssooss,sooooossssssssssoss, soooosssssssssoss, sooosssssssssooss,sooossssssssssoooss, or soosssssssssoooss.

The nucleobase sequences of SEQ ID Nos: 172, 764-770, 995, 1445, 1668,1743, 2255, 2595, 2762-2767 are complementary to a portion ofnucleobases 483,965-484,003 of SEQ ID NO: 1.

The nucleobase sequence of Compound Nos: 617557, 699781, 750138-750144,1065918-1065921, 1165621, 1165878, and 1263532-1263557 are complementaryto a portion of nucleobases 483,965-484,003 of SEQ ID NO: 1.

In certain embodiments, modified oligonucleotides complementary to aportion of nucleobases 483,965-484,003 of SEQ ID NO: 1 achieve at least24% reduction of UBE3A-ATS RNA in vitro in the standard cell assay. Incertain embodiments, modified oligonucleotides complementary tonucleobases 483,965-484,003 of SEQ ID NO: 1 achieve an average of 65%reduction of UBE3A-ATS RNA in vitro in the standard cell assay. Incertain embodiments, modified oligonculeotides complementary to aportion of nucleobases 468,968-469,013 of SEQ ID NO: 1 achieve anaverage of 330% upregulation of UBE3A-ATS RNA in vitro at 6.7 μM in cellculture.

4. Additional Hotspot Regions

In certain embodiments, the ranges described in the Table below comprisehotspot regions. Each hotspot region begins with the nucleobase of SEQID NO: 1 identified in the “Start Site SEQ ID NO: 1” column and endswith the nucleobase of SEQ ID NO: 1 identified in the “Stop Site SEQ IDNO: 1” column In certain embodiments, modified oligonucleotides arecomplementary within any of the hotspot regions 1-61, as defined in thetable below. In certain embodiments, modified oligonucleotides are 18nucleobases in length. In certain embodiments, modified oligonucleotidesare 20 nucleobases in length. In certain embodiments, modifiedoligonucleotides are gapmers. In certain embodiments, modifiedoligonucleotides are 4-8-6, 6-8-4, 5-8-5, 4-10-6, 6-10-4, or 5-10-5 MOEgapmers.

The nucleobase sequence of compounds listed in the “Compound ID inrange” column in the table below are complementary to SEQ ID NO: 1within the specified hotspot region. The nucleobase sequence of theoligonucleotides listed in the “SEQ ID NO: in range” column in the tablebelow are complementary to the target sequence, SEQ ID NO: 1, within thespecified hotspot region.

In certain embodiments, modified oligonucleotides complementary tonucleobases within the hotspot region achieve at least “Min. % Red ”(minimum % reduction, relative to untreated control cells) of UBE3A-ATSRNA in vitro in the standard cell assay, as indicated in the tablebelow. In certain embodiments, modified oligonucleotides complementaryto nucleobases within the hotspot region achieve an average of “Avg. %Red.” (average % reduction, relative to untreated control cells) ofUBE3A-ATS RNA in vitro in the standard cell assay, as indicated in thetable below. In certain embodiments, modified oligonucleotidescomplementary to nucleobases within the hotspot region achieve a maximumof “Max. % Red.” (maximum % reduction, relative to untreated controlcells) of UBE3A-ATS RNA in vitro in the standard cell assay, asindicated in the table below.

TABLE 1 UBE3A-ATS Hotspots _Hot- Start Site Stop Site Min. Max. Avg.spot SEQ ID SEQ ID % % % ID NO: 1 NO: 1 Red. Red. Red. Compound ID inrange SEQ ID NO: in range 1 461413 461487 36 89 60 749901-749904,1065641- 1053, 1329, 1501, 1576, 1065646, 1165562-1165563, 1873, 1949,2025, 2096, 1165857-1165858, 1273001 2245, 2512, 2591, 2680- 2682, 28442 468968 469013 75 81 78 750031-750032, 1263408- 376, 377, 2751-2756,1263411, 1263426, 1263441, 2773-2776, 2872, 2873, 1263460-1263465,1263486- 2876-2878 1263492, 1263517-1263523, 1273061, 1273062, 1273065-1273067 3 483965 484003 24 91 65 617557, 699781, 750138- 172, 764-770,995, 750144, 1065918-1065921, 1445, 1668, 1743, 2255, 1165621, 1165878,1263532- 2595, 2762-2767 1263557 4  349103#  349150# 35 82 56750519-750533 181-195 5 457354 457405 37 85 66 749848, 1065574-1065576,552, 973, 1273, 1795, 1165519-1165521, 2162, 2237, 2370, 2537,1179839-1179840 2671 6 457899 457936 49 77 59 749852, 1065581-1065582556, 1497, 1945 7 457969 458013 36 78 61 749853, 1065583-1065586, 557,1124, 1199, 1647, 1165523-1165525 2021, 2089, 2464, 2538 8 458523 45855864 77 71 749861-749862, 1065595 565, 566, 1050 9 458560 458627 45 81 64749863-749867, 1065596- 567-571, 1125, 1498, 1065600, 1272999 1573,1946, 2022, 2842 10 458935 458976 30 85 60 1065602-1065605, 901, 1425,1648, 1724, 1165530-1165540, 2091, 2092, 2164, 2165, 1165853 2239, 2240,2392-2393, 2465, 2511, 2539, 2540 11 460105 460137 43 82 60 749882,1065615-1065619, 586, 1126, 1201, 1649, 1165547-1165549 1725, 2023,2093, 2467, 2542 12 460348 460381 36 77 58 1065621-1065622, 902, 976,2167, 2242 1165550-1165551 13 460641 460686 50 67 57 617531,1065629-1065631 107, 1500, 1948, 2024 14 460974 461021 75 91 82749893-749894, 1165855, 597, 598, 2219, 2745- 1263451-1263455, 27491263473-1263478, 1263504-1263509 15 461597 461627 50 85 681065651-1065655 904, 978, 1278, 1428, 1800 16 463502 463531 60 71 66749931, 1065674-1065675 1503, 1578, 2708 17 463826 463872 50 71 62749938, 749939, 1065680 1728, 2714, 2715 18 464377 464428 32 77 58749951, 1065684-1065687, 1280, 1356, 1802, 1876, 1165572-1165577,1273006- 2097, 2172, 2247, 2398, 1273008 2471, 2546, 2727, 2849- 2851 19464522 464575 47 77 62 749952-749954, 1065690, 1504, 2098, 2728-2730,1165578, 1263412-1263416, 2757-2760, 2777-2781, 1263427-1263431,1263442- 2879-2881 1263446, 1263466-1263469, 1263494-1263499, 1263524-1263530, 1273068-1273070 20 464994 465032 27 76 52 617457,749957-749964, 33, 907, 981, 1281, 1065698-1065702, 1165866 1357, 1877,2136, 2733- 2739 21 465231 465266 23 83 63 749969, 1065706-1065713, 318,908, 1132, 1207, 1165583-1165587, 1263403- 1432, 1655, 1730, 1953,1263407, 1263421-1263425, 2028, 2174, 2248, 2400, 1263436-1263440,1263456, 2473, 2548, 2750, 2768- 1263479-1263485, 1263510- 2772, 28751263516, 1273063, 1273064 22 465372 465413 33 73 54 749972,1065717-1065719, 321, 1058, 1358, 1878, 1165588-1165592, 1165867- 2099,2137, 2175, 2249, 1165869 2401, 2475, 2514, 2593 23 465600 465631 65 6867 749975, 1065727-1065728, 324, 1433, 1731, 2787- 1272944-1272947 279024 466244 466282 38 68 54 1065742-1065744, 1165595- 1434, 1657, 1732,2176, 1165598 2251, 2403, 2549 25 466529 466570 31 80 57 617460,749989-749998, 36, 338-346, 911, 1065760-1065762 985, 1435 26 466979467014 51 75 65 617461, 750000-750005, 37, 347-351, 1806 1065764 27467043 467095 44 93 69 617539, 750006, 1065765- 115, 352, 1061, 1361,1065767, 1272943, 1272960, 1881, 2786, 2803, 2848 1273005 28 468335468372 42 68 56 1065781-1065783, 1165609, 1062, 1362, 1882, 2139,1165875 2405 29 475827 475877 39 71 57 617470, 1065830-1065834 46, 1065,1365, 1513, 1588, 1961 30 482148 482181 49 70 68 750122,1065889-1065890, 748, 919, 993, 2406 1165612 31 487589 487629 73 79 76750172, 1065953-1065955, 798, 997, 1297, 1818, 1272973, 1272974 2816,2817 32 487772 487826 44 73 62 750175-750177, 1065957 801-803, 1373 33489873 489927 52 73 59 750195-750196, 1065989 398, 399, 1375 34 493831493860 44 63 56 750202-750204 405-407 35 499081 499119 47 70 551066036-1066038 1078, 1378, 1898 36 500605 500658 36 66 58750265-750266, 1066064- 683, 684, 929, 1004 1066065 37 500846 500905 3086 55 750269-750270, 1066073- 687-688, 1155, 1230, 1066077, 11656701678, 1976, 2051, 2562 38 501335 501375 42 67 56 1066091-1066094,1272975- 1156, 1231, 1679, 1753, 1272977 2818-2820 39 502125 502157 6579 72 750291, 1066119-1066121 708, 1531, 1606, 1979 40 502194 502228 5188 65 750292, 1066124-1066125 709, 1233, 1681 41 502416 502452 49 59 541066130-1066132 1308, 1829, 1904 42 502580 502618 57 59 58 750295,1066140-1066142 712, 1234, 1682, 1756 43 503427 503461 36 73 61 750301,1066185, 1165724 718, 1983, 2197 44 503636 503675 55 72 62750307-750308, 1066190 724, 725, 1759 45 503973 504034 20 81 56617503-617505, 617581- 79-81, 155-157, 617582, 750313-750324, 302,731-736, 2059 750383, 1066202 46 504088 504122 45 85 65 750325-750327,1066206 245, 246, 737, 1760 47 504431 504460 49 70 57 1066218-1066219,1165736- 1164, 2060, 2119, 2276, 1165740 2427, 2495, 2574 48 506031506071 33 66 52 750356, 750357, 1066274- 275, 276, 1317, 1838, 10662761913 49 508739 508780 20 83 56 617503-617504, 617580- 79, 80, 155-157,617582, 750313-750323, 302, 730-735, 2059 750383, 1066202 50 509208509271 46 68 63 750394-750395, 1066377- 313-314, 1995, 2070 1066378 51510203 510247 36 77 60 750400-750401, 1066394- 810, 811, 1175, 2071,1066395, 1165828 2440 52 510832 510872 44 70 55 1066418-1066421 1326,1402, 1847, 1922 53 513442 513486 66 81 72 750436-750439 846-849 #Theoligonucleotides described in this section are complementary to thishotspot region at multiple sites. These sites are described in detail inTable 4b herein below (Example 1).

Nonlimiting Disclosure and Incorporation by Reference

Each of the literature and patent publications listed herein isincorporated by reference in its entirety.

While certain compounds, compositions and methods described herein havebeen described with specificity in accordance with certain embodiments,the following examples serve only to illustrate the compounds describedherein and are not intended to limit the same. Each of the references,GenBank accession numbers, and the like recited in the presentapplication is incorporated herein by reference in its entirety.

Although the sequence listing accompanying this filing identifies eachsequence as either “RNA” or “DNA” as required, in reality, thosesequences may be modified with any combination of chemicalmodifications. One of skill in the art will readily appreciate that suchdesignation as “RNA” or “DNA” to describe modified oligonucleotides is,in certain instances, arbitrary. For example, an oligonucleotidecomprising a nucleoside comprising a 2′-OH sugar moiety and a thyminebase could be described as a DNA having a modified sugar (2′-OH in placeof one 2′-H of DNA) or as an RNA having a modified base (thymine(methylated uracil) in place of an uracil of RNA). Accordingly, nucleicacid sequences provided herein, including, but not limited to those inthe sequence listing, are intended to encompass nucleic acids containingany combination of natural or modified RNA and/or DNA, including, butnot limited to such nucleic acids having modified nucleobases. By way offurther example and without limitation, an oligomeric compound havingthe nucleobase sequence “ATCGATCG” encompasses any oligomeric compoundshaving such nucleobase sequence, whether modified or unmodified,including, but not limited to, such compounds comprising RNA bases, suchas those having sequence “AUCGAUCG” and those having some DNA bases andsome RNA bases such as “AUCGATCG” and oligomeric compounds having othermodified nucleobases, such as “AT^(m)CGAUCG,” wherein ^(m)C indicates acytosine base comprising a methyl group at the 5-position.

Certain compounds described herein (e.g., modified oligonucleotides)have one or more asymmetric center and thus give rise to enantiomers,diastereomers, and other stereoisomeric configurations that may bedefined, in terms of absolute stereochemistry, as (R) or (S), as α or βsuch as for sugar anomers, or as (D) or (L), such as for amino acids,etc. Compounds provided herein that are drawn or described as havingcertain stereoisomeric configurations include only the indicatedcompounds. Compounds provided herein that are drawn or described withundefined stereochemistry include all such possible isomers, includingtheir stereorandom and optically pure forms, unless specified otherwise.Likewise, tautomeric forms of the compounds herein are also includedunless otherwise indicated. Unless otherwise indicated, compoundsdescribed herein are intended to include corresponding salt forms.

The compounds described herein include variations in which one or moreatoms are replaced with a non-radioactive isotope or radioactive isotopeof the indicated element. For example, compounds herein that comprisehydrogen atoms encompass all possible deuterium substitutions for eachof the 41 hydrogen atoms. Isotopic substitutions encompassed by thecompounds herein include but are not limited to: ²H or ³H in place of¹H, ¹³C or ¹⁴C in place of ¹²C, ¹⁵N in place of ¹⁴N, ¹⁷O or ¹⁸O in placeof ¹⁶O and ³³S, ³⁴S, ³⁵S, or ³⁶S in place of ³²S. In certainembodiments, non-radioactive isotopic substitutions may impart newproperties on the oligomeric compound that are beneficial for use as atherapeutic or research tool. In certain embodiments, radioactiveisotopic substitutions may make the compound suitable for research ordiagnostic purposes such as imaging.

EXAMPLES

The following examples illustrate certain embodiments of the presentdisclosure and are not limiting. Moreover, where specific embodimentsare provided, the inventors have contemplated generic application ofthose specific embodiments. For example, disclosure of anoligonucleotide having a particular motif provides reasonable supportfor additional oligonucleotides having the same or similar motif. And,for example, where a particular high-affinity modification appears at aparticular position, other high-affinity modifications at the sameposition are considered suitable, unless otherwise indicated.

Example 1 Effect of 5-10-5 MOE Gapmer Modified Oligonucleotides on HumanUBE3A-ATS RNA In Vitro, Single Dose

Modified oligonucleotides complementary to human UBE3A-ATS nucleic acidwere tested for their effect on UBE3A-ATS RNA levels in vitro.

The modified oligonucleotides in the tables below are 5-10-5 MOEgapmers. The gapmers are 20 nucleosides in length, wherein the centralgap segment consists of ten 2′-β-D-deoxynucleosides and the 5′ and 3′wing segments each consists of five 2′-MOE nucleosides. The sugar motiffor the gampers is (from 5′ to 3′): eeeeeddddddddddeeeee; wherein ‘0:1’represents a 2′-β-D-deoxyribosyl sugar moiety, and ‘e’ represents a2′-MOE sugar moiety.

Each internucleoside linkage of Compound IDs 617441-617596 (in Tables 2and 3) is a phosphorothioate internucleoside linkage. All othercompounds (Tables 4-33) have an internucleoside linkage motif of (from5′ to 3′): soooossssssssssooss, wherein each “s” represents aphosphorothioate internucleoside linkage and each “o” represents aphosphodiester internucleoside linkage.

All cytosine residues are 5-methylcytosines.

“Start site” indicates the 5′-most nucleoside to which the modifiedoligonucleotide is complementary in the human gene sequence. “Stop site”indicates the 3′-most nucleoside to which the modified oligonucleotideis complementary in the target nucleic acid sequence. Each modifiedoligonucleotide listed in the Tables below is 100% complementary to SEQID NO: 1 (GENBANK Accession No NC_000015.10 truncated from nucleotides24821647 to 25441028). Selected compounds in the table below arecomplementary to the target nucleic acid sequence at more than threespecific sites. For these compounds, the “start site” and “stop site”values in the Tables below are indicated with a hashtag (#) and indicateonly the first site to which the compound is complementary. Additionalsites to which these compounds are complementary are indicated in Table4b below.

Human IPSC cell derived iCell GABANeurons (Cellular Dynamics) werecultured per manufacturer instructions at 20,000-60,000 cells per well(as indicated in the table heading) and were treated with 5,000-10,000nM of modified oligonucleotide (as indicated in the table heading) byfree uptake. After a treatment period of approximately 6 days, total RNAwas isolated from the cells and UBE3A-ATS RNA levels were measured byquantitative real-time RTPCR. Human UBE3A-ATS primer probe set RTS4796(forward sequence CTCCCCCAGTTCTGGAATGA, designated herein as SEQ ID NO:2; reverse sequence TACACAGGGATTTGAGCCTGCTA, designated herein as SEQ IDNO: 3; probe sequence CCCACAGATCAAGCATTCCCCAAAGA, designated herein asSEQ ID NO: 4) was used to measure RNA levels. UBE3A-ATS RNA levels werenormalized to total RNA content, as measured by RIBOGREEN®. Reduction ofUBE3A-ATS RNA is presented in the tables below as percent UBE3A-ATS RNAamount relative to untreated control (UTC) cells. Each table representsresults from an individual assay plate. The values marked with anasterisk (*) indicate that the modified oligonucleotide is complementaryto the amplicon region of the primer probe set. Additional assays may beused to measure the potency and efficacy of the modifiedoligonucleotides complementary to the amplicon region. “N.D.” indicatesthat a value was not determined in this experiment due to experimentalerror. However, activities of selected modified oligonucleotides,including those that are not defined in Example 1, are successfullydemonstrated in dose-response studies herein below.

TABLE 2 Reduction of UBE3A-ATS RNA by 7,000 nM 5-10-5 MOE gapmers withPS internucleoside linkages in vitro (60,000 cells/well) SEQ ID SEQ IDCompound NO: 1 NO: 1 UBE3A-ATS SEQ ID Number Start Site Stop SiteSequence (5′ to 3′) (% UTC) NO 617441 449466 449485 ATGTTGCTTGCACTCCATCA85 17 617442 449945 449964 ACAACATAAGGTCTTATTGT 87 18 617443 450581450600 TTTTCAACTCCAGAATTTTC 87 19 617444 451644 451663TTCAGTCTTATACAGAAATG 94 20 617445 452473 452492 GGACTGACACAGAAAACTGG 11521 617446 454843 454862 AGGGTAGAAGACTAGCATAC 111 22 617447 455272 455291GGCCATTCATTCAGCCACAC 95 23 617448 455405 455424 TAAAGATTTTATGAAAATAC 10424 617449 456040 456059 TTTAGAACATGTGAATTTCA 109 25 617450 457025 457044AGACATATCACAGTTGCTTG 89 26 617451 457601 457620 AATATAATGTAGTATGACCA 5927 617452 458989 459008 GAGGACACTGGCACATCTAT 59 28 617453 459376 459395TTAAATAATAAAATATATTT 108 29 459401 459420 617454 461190 461209ATTGGAGCAAAAAGGGATCA 45 30 617455 462327 462346 AATTATTCCCTATATCCTGT 4531 617456 463905 463924 CAACTGTTACCAAGACTTCA 24 32 617457 465003 465022ATCTGCGAATAGAGGCCCTC 44 33 617458 465460 465479 AAGGTTTTTATTTCATCACT 6834 617459 465872 465891 GCCCATGGATGGTTGTCAAA 34 35 617460 466539 466558TAGGTTGCATAAAGCCAGGC 23 36 617461 466981 467000 CACACATCTTGTTCCCTCAA 2337 617462 467508 467527 TTTTTTTAATCCTCTGATGA 123 38 617463 467914 467933AGTCTCTTTTCTTTCGGTGC 34 39 617464 468085 468104 GGAAAGTTCTTCTCTCTCCT 6940 617465 469204 469223 ATTTGGTCTAAAGTGAAGTT 91 41 617466 470003 470022TTTTGTGAGATTTTTGAATA 101 42 617467 471815 471834 TGTTAAATGCTTTTCTAAAT 8043 617468 472985 473004 CTGATGATTGATTGTTTCCT 71 44 617469 474868 474887TATGATAATGTGTAGTTTTT 110 45 617470 475850 475869 AAGGGTAATACGGACCTCAT 2946 617471 476475 476494 CTATTTTTTGCTTCCCTTAT 56 47 617472 479448 479467GAATTCTTAGAAAGTTAATT 118 48 617473 479585 479604 TGCCATCTTCAAGACTAAGG 3349 617474 480527 480546 AATGGAAAAGATGTATCACG 92 50 617475 481063 481082AACTTTGGCAGCTATTCAAT 88 51 617476 481522 481541 GACACTAGGTTTTGCAAAAG 6352 617477 481663 481682 TATGAATTTTCAATTCAATG 155 53 617478 483415 483434CACTTAAGGGAACTTTCAGA 84 54 617479 483933 483952 GAGATGATGTTAAGCTTTCA 7555 617480 484344 484363 TAAGACAAGTGGCCATGAAG 92 56 617481 484760 484779CTCTGTTCTGTCTCAAAACA 47 57 617482 485496 485515 TATAAGAAAGAAGATTACAG 13258 617483 486358 486377 AAATGTTAATAGACTGCGAT 93 59 617484 486941 486960TTAAACTCCCCAGTCAAAAG 108 60 617485 488180 488199 ATTTACTTCAAACAGGAGCT 4261 617486 489476 489495 TCAGGAAATTAAAGCATTCA 81 62 617487 489630 489649CCTAACCTGGATCTCAGATA 57 63 617488 493552 493571 TACAGCAACCCTAGAAAACT 12164 617489 493893 493912 ATCTTTGGCAGATGTAACCT 63 65 617490 495750 495769GAGTATTATCCAAAAGAACA 83 66 617491 497275 497294 AAAGCCCAGGATTAGGCAGC 6367 617492 497366 497385 CTACAAAGAGTGAATCATGA 130 68 617493 498003 498022TAATCTTAAGTTTAAGTGGA 68 69 617494 498883 498902 AAAAAGACATAGAATGACAG 10070 617495 499237 499256 AGATACAAATTTAAAAAAGT 119 71 617496 499672 499691GGTAGCCCCAATACAGATTC 62 72 617497 500044 500063 CTTCAACAGCAGACTTGATC 6573 617498 501513 501532 GGTCCACACAGAGTTCAAAT 73 74 617499 501703 501722CTTTGGGACATCCCAAAGTT 113 75 617500 502388 502407 TACACATCTTGTATACAAGG 6076 617501 503258 503277 TAATACTTTCTTGAGTAATA 124 77 617502 503904 503923GGGTGGTTGGATTGCTTTAT 47 78 617503 503977 503996 CTGCACACTGTACAGGAGGG 8079 508743 508762 617504 503985 504004 TTGATTCACTGCACACTGTA 67 80 508751508770 617505 504015 504034 GTGCAGGAAGGAGGTTTTGT 55 81 617506 504943504962 ATACAGTATATACATCATCC 42 82 617507 505266 505285AAGTTTAAAACAAAAAAAGG 119 83 617508 507068 507087 AATGTCTGTTCTTTGTGGTA 9184 617509 507897 507916 ATACCTGCTTTTGTGACAAT 75 85 617510 508556 508575GAGTGTAGCTCATTTCAGAA 74 86 617511 509785 509804 TTTCTCAATGTCAACTCTCA 6887 617512 510721 510740 CAAGCCGAATCTTGACATAC 73 88 617513 511227 511246GACCAGAAAAGCTACATAGC 87 89 617514 512575 512594 ATTTTGTATATTGTAGCTTT 10590 617515 513295 513314 CCATCTTTCTGTTATCTTGT 51 91 617516 513982 514001CTTTGTTTCTTTTTAAGAAA 108 92 617517 514259 514278 ACCTTGACAACACCCTAGCT 8993 617518 515123 515142 AAAAAGGAACCATAAACTAA 117 94

TABLE 3 Reduction of UBE3A-ATS RNA by 7,000 nM 5-10-5 MOE gapmers withPS internucleoside linkages in vifro (60,000 cells/well) SEQ ID SEQ IDCompound NO: 1 NO: 1 UBE3A-ATS SEQ ID Number Start Site Stop SiteSequence (5′ to 3′) (% UTC) NO 617461 466981 467000 CACACATCTTGTTCCCTCAA29 37 617519 449812 449831 GGTGTGTCAGCTGTGCTGGT 62 95 617520 450498450517 ATCATACCTTCACTTTTTTT 101 96 617521 450896 450915TTCATTGAGCTTCCTGGATA 100 97 617522 451804 451823 TTACTTCTTTTTCATTAAGT 8698 617523 454628 454647 AGTAAACACTCCAACAAAAA 126 99 617524 455049 455068GGTCTAAGCAAAATGTGAAG 137 100 617525 455323 455342 CAGTTAGGCCTGCTCCGAAT102 101 617526 455499 455518 ACTCTGAAAATAGCCAATTT 134 102 617527 456944456963 AGTATCTATCTACAATTAAA 112 103 617528 457237 457256AATGGCTTAGCTACTCACCC 72 104 617529 458254 458273 CCTCTCTGCAAAACAAGAGA 74105 617530 459153 459172 ATTACTCATTCTGGGAATGC 35 106 617531 460667460686 GTTTTCAGCATGATTCTAAC 46 107 617532 461259 461278CATATATAAAAATTAGAATG 122 108 617533 463621 463640 AATGCCACAAAGCTGGCTGT97 109 617534 464514 464533 GTGCACAGATAATGACTAGA 69 110 617535 465402465421 CAGTGAGAAGTCAATTGTCA 67 111 617536 465580 465599TCCTTTAGCATTTCTATTAG 28 112 617537 466423 466442 AACATGTGCTTGCAAGCCAT 46113 617538 466701 466720 AAATAACTGGATCTCATAAC 55 114 617539 467064467083 CTTAGGAGAGAAACACTTTC 56 115 617540 467546 467565TACATCCTGTAGGCTTTCTT 55 116 617541 467961 467980 ACTGAGAAATCTCTTGAATG 92117 617542 468624 468643 TTGCTCTTTTTACTTTGTTC 37 118 617543 469731469750 ACTCTAAGTTTTATTAATAG 105 119 617544 470091 470110TTTGGAAGAGCTTAATAAAG 110 120 617545 472023 472042 TAGCTAGAATTTCAACTACT63 121 617546 474443 474462 ATGTAGTATTTATTTCATTT 120 122 617547 474957474976 GCCTAATATGTGTCATCCTG 28 123 617548 475998 476017CCTCAATTCTATGGTTAGTT 52 124 617549 479376 479395 TCTTGGAGATGACTTCTCTG 75125 617550 479580 479599 TCTTCAAGACTAAGGTAGGG 54 126 617551 479654479673 AGTAAGGTCTGTTATTCTCC 35 127 617552 480998 481017GCTATACAACAAAAAGAATT 123 128 617553 481073 481092 ACTGTGGAAAAACTTTGGCA54 129 617554 481582 481601 ATAATCTACATGTATAGACC 85 130 617555 481984482003 AAAAGCCAATTCTGAAATTC 44 131 617556 483689 483708ATTCTCCTACCTCTCAGCCT 107 132 617558 484654 484673 AACTGTAGCAAATATACTAC110 133 617559 485236 485255 AAATTACTGCTCTGTAAAAG 93 134 617560 485886485905 GATTCAATGAAATAAAAAAT 144 135 617561 486894 486913AATGACATAGCTTATGCTGT 104 136 617562 488162 488181 CTAGAAATTAAGACATCCCT63 137 617563 488563 488582 GCCACACCATCAAAAGAACC 77 138 617564 489518489537 TGGACCACCTAAGACCTCAA 62 139 617565 489731 489750ACCGGTTCCCAATTTTCTCC 82 140 617566 493655 493674 GAGAGAATACGGCCCTGATG 77141 617567 494790 494809 AACTTCAATCAGAGTAATAT 117 142 617568 496045496064 GGCAAAGGAATGAAGAGACC 69 143 617569 497328 497347GCCAATAACAAGAAAAGAGA 96 144 617570 497416 497435 ATCAAATTGGATGACCTAAA122 145 617571 498140 498159 CAATGGATTTCAATTACACT 54 146 617572 498893498912 ATTCTCCAACAAAAAGACAT 93 147 617573 499276 499295GTAGCTACAAGAAGTAATTG 117 148 617574 499958 499977 AAAGAATGCCTATAAGAATT118 149 617575 500578 500597 CTACTGGCATCAGTCAAAAC 60 150 617576 501578501597 AAAAGGCAAGGCTAAGGAGT 69 151 617577 502305 502324CCCACACAGGTCATTCATTC 52 152 617578 503150 503169 TGTATACACCATCCCAGAAA 93153 617579 503795 503814 AAAATGCCAGTTTGTTGTAC 60 154 617580 503973503992 ACACTGTACAGGAGGGTGTC 69 155 508739 508758 617581 503981 504000TTCACTGCACACTGTACAGG 70 156 508747 508766 617582 503993 504012AAATGATGTTGATTCACTGC 36 157 508759 508778 617583 504551 504570AGAGTTTTCATGAATTCAGG 46 158 617584 505011 505030 GGTTTCTTTCATTAAATAGC 63159 617585 506396 506415 GAGGCAACTCCTGAGAGCTG 64 160 617586 507650507669 TTAAATGTCAGGAGGTCCCC 57 161 617587 508201 508220TGTCATCTGATCTCACACAT 86 162 617588 508772 508791 CAAAGTCCAAGGGAAATGAT 71163 617589 510010 510029 CACAGTAGAGGCAAATGAGA 79 164 617590 511058511077 GACATTTAGGATGATGATAT 72 165 617591 512158 512177GTTGTATTAATGGACTTTTG 65 166 617592 513203 513222 ATTTTTTGCATAAGTGCATT 86167 617593 513508 513527 ACATTTCTTAGTTGAACAGT 29 168 617594 514104514123 AGATTCTTCCCCAATCCCAT 81 169 617595 514524 514543CTCACTTGTCTCCTTTTACC 73 170 617596 515282 515301 ACAATAAGGAAGAGCAAAAC104 171

TABLE 4 Reduction of UBE3A-ATS RNA by 5,000 nM 5-10-5 MOE gapmers withmixed PO/PS internucleoside linkages in vitro (35,000 cells/well) SEQ IDSEQ ID Compound NO: 1 NO: 1 UBE3A-ATS SEQ ID Number Start Site Stop SiteSequence (5′ to 3′) (% UTC) NO 617456 463905 463924 CAACTGTTACCAAGACTTCA22 32 617457 465003 465022 ATCTGCGAATAGAGGCCCTC 22 33 617460 466539466558 TAGGTTGCATAAAGCCAGGC 35 36 617461 466981 467000CACACATCTTGTTCCCTCAA 36 37 617557 483977 483996 ATCATGTGCATACCCAGGGT 9172 705110 229870^(#) 229889^(#) TCACTCATTTTGTTCAGCTT 33 173 705112232607^(#) 232626^(#) GTTTTCACTCATTTTGTTCA 61 174 750513 220331 220350ACAATTATTCTCATCATCGA 113 175 456700 456719 750514 220346 220365CCTCAGCATCCTCAGACAAT 80 176 456715 456734 750515 220359 220378TCTGGAATGAGTCCCTCAGC 73 177 456728 456747 750516 220371 220390CTCAGATTGACATCTGGAAT 123 178 456740 456759 750517 229869^(#) 229888^(#)CACTCATTTTGTTCAGCTTT 15 179 750518 232608^(#) 232627^(#)AGTTTTCACTCATTTTGTTC 67 180 750519 349103^(#) 349122^(#)GTCATCACCTCTCTTCAGGA 22 181 750520 349105^(#) 349124^(#)AAGTCATCACCTCTCTTCAG 35 182 750521 349107^(#) 349126^(#)TTAAGTCATCACCTCTCTTC 55 183 750522 349109^(#) 349128^(#)TTTTAAGTCATCACCTCTCT 37 184 750523 349111^(#) 349130^(#)ATTTTTAAGTCATCACCTCT 45 185 750524 349113^(#) 349132^(#)TGATTTTTAAGTCATCACCT 25 186 750525 349115^(#) 349134^(#)CATGATTTTTAAGTCATCAC 55 187 750526 349117^(#) 349136^(#)AGCATGATTTTTAAGTCATC 65 188 750527 349119^(#) 349138^(#)TGAGCATGATTTTTAAGTCA 46 189 750528 349121^(#) 349140^(#)ATTGAGCATGATTTTTAAGT 56 190 750529 349123^(#) 349142^(#)CTATTGAGCATGATTTTTAA 60 191 750530 349125^(#) 349144^(#)TCCTATTGAGCATGATTTTT 56 192 750531 349127^(#) 349146^(#)AATCCTATTGAGCATGATTT 41 193 750532 349129^(#) 349148^(#)GTAATCCTATTGAGCATGAT 26 194 750533 349131^(#) 349150^(#)GCGTAATCCTATTGAGCATG 34 195 750534 349133^(#) 349152^(#)CAGCGTAATCCTATTGAGCA 63 196 750535 349135^(#) 349154^(#)CTCAGCGTAATCCTATTGAG 50 197 750536 349137^(#) 349156^(#)GCCTCAGCGTAATCCTATTG 71 198 750537 349139^(#) 349158^(#)GGGCCTCAGCGTAATCCTAT 48 199 750538 349141^(#) 349160^(#)CTGGGCCTCAGCGTAATCCT 28 200 750539 349143^(#) 349162^(#)GGCTGGGCCTCAGCGTAATC 75 201 750540 355254^(#) 355273^(#)TAGGCTGGGCCTCAGCGTAA 32 202 750541 349147^(#) 349166^(#)CCTAGGCTGGGCCTCAGCGT 98 203 750542 349149^(#) 349168^(#)CACCTAGGCTGGGCCTCAGC 21 204 750543 349151^(#) 349170^(#)CTCACCTAGGCTGGGCCTCA 55 205 750544 349153^(#) 349172^(#)TTCTCACCTAGGCTGGGCCT 24 206 750545 349155^(#) 349174^(#)AATTCTCACCTAGGCTGGGC 53 207 750546 349157^(#) 349176^(#)AAAATTCTCACCTAGGCTGG 39 208 750547 349159^(#) 349178^(#)CCAAAATTCTCACCTAGGCT 46 209 750548 349162^(#) 349181^(#)CTTCCAAAATTCTCACCTAG 95 210 750549 349165^(#) 349184^(#)CCTCTTCCAAAATTCTCACC 17 211 750550 349168^(#) 349187^(#)CATCCTCTTCCAAAATTCTC 75 212 750551 349171^(#) 349190^(#)CAGCATCCTCTTCCAAAATT 50 213 750552 349174^(#) 349193^(#)TCCCAGCATCCTCTTCCAAA 70 214 750553 349177^(#) 349196^(#)GGATCCCAGCATCCTCTTCC 53 215 750554 349346 349365 GCCACCCACATGCCCTGCCC 25216 409462 409481 750555 349390^(#) 349409^(#) AGAGCTCACTGAAAGACACA 63217 750556 349392^(#) 349411^(#) GAAGAGCTCACTGAAAGACA 85 218 750557349442^(#) 349461^(#) GCAGGATCCACTCACCTATG 27 219 750558 349957^(#)349976^(#) CAGAGCTCAGCCTTGACCCA 45 220 750559 350910^(#) 350929^(#)AGCTCAGTGCAGGAGACCAG 56 221 750560 350914^(#) 350933^(#)CCACAGCTCAGTGCAGGAGA 72 222 750561 350924^(#) 350943^(#)GATGTGCTCACCACAGCTCA 115 223 750562 351544^(#) 351563^(#)GGAACCCTTTCCTGCCTGGA 56 224 750563 353287^(#) 353306^(#)CAATATAAGGTTCTCATCAT 42 225 750564 353293^(#) 353312^(#)TCAGGACAATATAAGGTTCT 115 226 750565 353305^(#) 353324^(#)CATCACCTCTCTTCAGGACA 37 227 750566 353356 353375 TCTCACCTAGGCTAGGCCTC 44228 407396 407415 750567 353579^(#) 353598^(#) AGCTCACTGAAAGACACAAG 49229 750568 358746^(#) 358765^(#) AGGAAGGGCCTGAGCTTCAG 34 230 750569363914 363933 CTCACCACACCTCAGTGCAG 93 231 384545 384564 750570364549^(#) 364568^(#) CTTTCCTGCCTGGACCACCA 68 232 750571 366064 366083TTGGGCACCCTCCAGATGCC 63 233 373449 373468 750572 366247 366266CACTCACCTATGCTGGTCAA 72 234 370104 370123 386794 386813 750573 366487366506 TCAGCTGGGTGCTGCCCTGC 117 235 407918 407937 750574 366668 366687CCATTCAGGGCCATGGGTTT 67 236 374053 374072 750575 391964 391983TCCCTGCACATGCATCCAGC 97 237 393859 393878 403520 403539 750576 395822395841 ACTCCAGGGACCAAGAGCTC 49 238 420812 420831 750577 397780 397799GAGACCCACTGAGATGGCCC 26 239 415262 415281 750578 402885 402904ACCCCATGACCCTGGAGGTG 63 240 410060 410079 750579 403932 403951ATGACCCCAGCAGGATGCAC 57 241 424209 424228 750580 422678 422697TCACCCACCACTGTCCAGAG 95 242 426410 426429 750581 422778 422797GTACCACTGAGATGGCCCAT 68 243 426510 426529 750582 423273 423292AATGCAGACCTGGCAGTCGC 50 244 430180 430199

TABLE 4b SEQ ID NO: 1 start sites for modified oligonucleotidescomplementary to repeat regions # of comp. Compound sites within SEQNumber SEQ ID NO: 1 SEQ ID NO: 1 start sites ID NO: 705110 20 229870,232603, 235253, 237932, 240726, 243420, 246181, 248825, 251500, 173258532, 259677, 261158, 261978, 263775, 264917, 266052, 267194, 268313,270176, 272427 705112 16 232607, 237936, 243424, 252518, 254323, 258536,259681, 261162, 261982, 174 263779, 264921, 266056, 267198, 268317,270180, 272431 750517 21 229869, 232602, 235252, 237931, 240725, 243419,246180, 248824, 251499, 179 257447, 258531, 259676, 261157, 261977,263774, 264916, 266051, 267193, 268312, 270175, 272426 750518 16 232608,237937, 243425, 252519, 254324, 258537, 259682, 261163, 261983, 180263780, 264922, 266057, 267199, 268318, 270181, 272432 750519 20 349103,353307, 357118, 364011, 367794, 369796, 371701, 377828, 379703, 181381607, 382737, 384642, 388298, 393921, 396997, 401626, 414465, 420126,421994, 423858, 427578 750520 30 349105, 353309, 355214, 357120, 358879,364013, 365917, 367796, 369798, 182 371703, 373302, 375957, 377830,379705, 381609, 382739, 384644, 386464, 388300, 393923, 396999, 400729,401628, 410769, 414467, 420128, 421996, 423860, 425727, 427580 750521 33349107, 351019, 353311, 355216, 357122, 358881, 364015, 365919, 367798,183 369800, 371705, 373304, 375959, 377832, 379707, 381611, 382741,384646, 386466, 388302, 390180, 393925, 397001, 400731, 401630, 410771,412631, 414469, 420130, 421998, 423862, 425729, 427582 750522 33 349109,351021, 353313, 355218, 357124, 358883, 364017, 365921, 367800, 184369802, 371707, 373306, 375961, 377834, 379709, 381613, 382743, 384648,386468, 388304, 390182, 393927, 397003, 400733, 401632, 410773, 412633,414471, 420132, 422000, 423864, 425731, 427584 750523 31 349111, 351023,353315, 355220, 357126, 358885, 364019, 365923, 367802, 185 369804,371709, 373308, 375963, 377836, 379711, 381615, 382745, 384650, 386470,388306, 393929, 397005, 401634, 410775, 412635, 414473, 420134, 422002,423866, 425733, 427586 750524 32 349113, 351025, 353317, 355222, 357128,358887, 364021, 365925, 367804, 186 369806, 371711, 373310, 375965,377838, 379713, 381617, 382747, 384652, 386472, 388308, 393931, 397007,401636, 405499, 410777, 412637, 414475, 420136, 422004, 423868, 425735,427588 750525 30 349115, 351027, 353319, 355224, 357130, 358889, 364023,365927, 367806, 187 369808, 371713, 373312, 375967, 377840, 384654,386474, 388310, 393933, 397009, 401638, 405501, 410779, 412639, 414477,420138, 422006, 423870, 425737, 427590, 429251 750526 31 349117, 351029,353321, 355226, 357132, 358891, 362700, 364025, 365929, 188 367808,369810, 371715, 373314, 375969, 377842, 384656, 386476, 388312, 397011,401640, 405503, 410781, 412641, 414479, 418232, 420140, 422008, 423872,425739, 427592, 429253 750527 31 349119, 351031, 355228, 357134, 358893,362702, 364027, 365931, 367810, 189 369812, 371717, 373316, 375971,377844, 384658, 386478, 388314, 392044, 397013, 401642, 405505, 410783,412643, 414481, 418234, 420142, 422010, 423874, 425741, 427594, 429255750528 32 349121, 351033, 355230, 357136, 358895, 360780, 362704,364029, 365933, 190 367812, 369814, 371719, 373318, 375973, 377846,384660, 386480, 388316, 392046, 397015, 401644, 405507, 410785, 412645,414483, 418236, 420144, 422012, 423876, 425743, 427596, 429257 750529 32349123, 351035, 355232, 357138, 358897, 362706, 364031, 365935, 367814,191 369816, 371721, 373320, 375975, 377848, 384662, 386482, 388318,392048, 397017, 401646, 403603, 405509, 410787, 412647, 414485, 418238,420146, 422014, 423878, 425745, 427598, 429259 750530 33 349125, 349125,351037, 355234, 357140, 358899, 362708, 364033, 365937, 192 367816,369818, 371723, 373322, 375977, 377850, 384664, 386484, 388320, 392050,397019, 401648, 403605, 405511, 410789, 412649, 414487, 418240, 420148,422016, 423880, 425747, 427600, 429261 750531 32 349127, 351039, 355236,357142, 358901, 362710, 364035, 365939, 367818, 193 369820, 371725,373324, 375979, 377852, 384666, 386486, 388322, 392052, 397021, 401650,403607, 405513, 410791, 412651, 414489, 418242, 420150, 422018, 423882,425749, 427602, 429263 750532 31 349129, 355238, 357144, 358903, 362712,364037, 365941, 367820, 369822, 194 371727, 373326, 375981, 377854,386488, 388324, 392054, 397023, 398903, 401652, 403609, 405515, 410793,412653, 414491, 418244, 420152, 422020, 423884, 425751, 427604, 429265750533 28 349131, 355240, 357146, 358905, 362714, 364039, 365943,367822, 369824, 195 371729, 373328, 375983, 377856, 386490, 388326,392056, 397025, 401654, 403611, 410795, 414493, 418246, 420154, 422022,423886, 425753, 427606, 429267 750534 29 349133, 355242, 357148, 358907,362716, 364041, 365945, 367824, 369826, 196 371731, 373330, 375985,377858, 386492, 388328, 392058, 397027, 401656, 403613, 409248, 410797,414495, 418248, 420156, 422024, 423888, 425755, 427608, 429269 750535 29349135, 355244, 357150, 358909, 362718, 364043, 365947, 367826, 369828,197 371733, 373332, 375987, 377860, 386494, 388330, 392060, 397029,401658, 403615, 409250, 410799, 414497, 418250, 420158, 422026, 423890,425757, 427610, 429271 750536 30 349137, 355246, 357152, 358911, 362720,364045, 365949, 367828, 369830, 198 371735, 373334, 375989, 377862,386496, 392062, 393955, 397031, 401660, 403617, 407381, 409252, 410801,414499, 418252, 420160, 422028, 423892, 425759, 427612, 429273 750537 30349139, 355248, 357154, 358913, 360801, 362722, 364047, 365951, 367830,199 369832, 371737, 373336, 375991, 377864, 386498, 392064, 393957,397033, 401662, 403619, 409254, 410803, 414501, 418254, 420162, 422030,423894, 425761, 427614, 429275 750538 25 349141, 355250, 357156, 358915,360803, 362724, 365953, 367832, 369834, 200 371739, 373338, 375993,377866, 392066, 393959, 397035, 401664, 403621, 410805, 418256, 420164,422032, 425763, 427616, 429277 750539 22 349143, 355252, 357158, 358917,362726, 365955, 369836, 373340, 375995, 201 377868, 392068, 393961,397037, 401666, 403623, 410807, 418258, 420166, 422034, 425765, 429279,448197 750540 20 349145, 355254, 358919, 362728, 365957, 369838, 373342,375997, 377870, 202 392070, 393963, 401668, 403625, 410809, 418260,420168, 422036, 425767, 429281, 448199 750541 18 349147, 355256, 358921,362730, 365959, 369840, 373344, 375999, 377872, 203 393965, 401670,410811, 418262, 420170, 422038, 425769, 429283, 448201 750542 17 349149,351061, 355258, 358923, 362732, 365961, 369842, 373346, 376001, 204377874, 393967, 410813, 418264, 420172, 422040, 425771, 429285 750543 16349151, 351063, 355260, 358925, 362734, 365963, 369844, 373348, 376003,205 377876, 393969, 418266, 420174, 422042, 425773, 429287 750544 15349153, 351065, 355262, 358927, 362736, 365965, 369846, 373350, 376005,206 377878, 418268, 420176, 422044, 425775, 429289 750545 14 349155,351067, 355264, 358929, 362738, 365967, 369848, 373352, 376007, 207377880, 418270, 420178, 425777, 429291 750546 13 349157, 351069, 355266,358931, 362740, 365969, 369850, 373354, 376009, 208 418272, 420180,425779, 429293 750547 14 349159, 351071, 355268, 358933, 362742, 365971,369852, 373356, 376011, 209 407403, 418274, 420182, 425781, 429295750548 17 349162, 351074, 355271, 358936, 362745, 365974, 369855,373359, 376014, 210 407406, 412686, 416425, 418277, 420185, 425784,427637, 429298 750549 12 349165, 351077, 357180, 358939, 362748, 365977,373362, 376017, 412689, 211 418280, 425787, 429301 750550 9 349168,351080, 357183, 358942, 365980, 373365, 376020, 412692, 425790 212750551 11 349171, 351083, 357186, 358945, 365983, 373368, 376023,381675, 405557, 213 412695, 425793 750552 14 349174, 351086, 358948,360836, 365986, 371772, 373371, 376026, 377898, 214 381678, 393992,412698, 422065, 425796 750553 10 349177, 351089, 358951, 371775, 377901,381681, 384716, 412701, 422068, 215 425799 750555 23 349390, 351292,353581, 355499, 361054, 364302, 368083, 370059, 371989, 217 373589,378120, 379994, 383026, 388586, 390466, 401917, 403871, 409508, 411053,412917, 414751, 420414, 429518 750556 24 349392, 351294, 353583, 355501,361056, 364304, 368085, 370061, 371991, 218 373591, 376253, 378122,383028, 388588, 390468, 401919, 403873, 409510, 411055, 412919, 420416,422284, 426016, 429520 750557 13 349442, 353633, 366255, 373640, 378173,390519, 397322, 401968, 407684, 219 409560, 414803, 420466, 429569750558 7 349957, 359696, 374917, 376821, 383615, 389153, 411626 220750559 12 350910, 358772, 365811, 373197, 375845, 403478, 407243,410663, 423753, 221 425620, 427479, 429146 750560 12 350914, 358776,365815, 373201, 375849, 390074, 403482, 410667, 423757, 222 425624,427483, 429150 750561 10 350924, 358786, 360674, 365825, 375859, 390084,401536, 409127, 425634, 223 427493 750562 10 351544, 353840, 361305,363233, 397528, 399421, 413175, 415009, 418768, 224 424402 750563 20353287, 357098, 358857, 363991, 365896, 367774, 369776, 379683, 381587,225 382717, 384622, 388278, 398857, 401606, 403563, 414445, 416346,425705, 427558, 429219 750564 20 353293, 357104, 360748, 363997, 367780,369782, 379689, 381593, 382723, 226 384628, 388284, 393907, 396983,398863, 401612, 405475, 414451, 416352, 427564, 429225 750565 17 353305,357116, 364009, 367792, 369794, 379701, 381605, 382735, 384640, 227388296, 393919, 396995, 401624, 414463, 420124, 423856, 427576 750567 12353579, 361052, 370057, 378118, 388584, 390464, 401915, 409506, 412915,229 414749, 420412, 429516 750568 11 358746, 362555, 365785, 369656,375819, 381477, 382607, 386333, 390044, 230 416238, 421864 750570 11364549, 385176, 392568, 397522, 399415, 404124, 407883, 413169, 415003,232 418762, 428112

TABLE 5 Reduction of UBE3A-ATS RNA by 7,000 nM 5-10-5 MOE gapmers withmixed PO/PS internucleoside linkages in vitro (35,000 cells/well) SEQ IDSEQ ID Compound NO: 1 NO: 1 UBE3A-ATS SEQ ID Number Start Site Stop SiteSequence (5′ to 3′) (% UTC) NO 617456 463905 463924 CAACTGTTACCAAGACTTCA40 32 617457 465003 465022 ATCTGCGAATAGAGGCCCTC 30 33 617460 466539466558 TAGGTTGCATAAAGCCAGGC 40 36 617461 466981 467000CACACATCTTGTTCCCTCAA 41 37 617557 483977 483996 ATCATGTGCATACCCAGGGT 26172 750326 504100 504119 CTTCAACTAACAGTATCTTA 20 245 750327 504103504122 AGTCTTCAACTAACAGTATC 51 246 750328 504126 504145CTATTTCATTAAGTCACCCC 53 247 750329 504179 504198 GGACAGCTGTGTGGAGGGAT 26248 750330 504218 504237 ACCACCAGGGAGACCAGCCT 68 249 750331 504228504247 CAGCCTTTCTACCACCAGGG 55 250 750332 504229 504248TCAGCCTTTCTACCACCAGG 32 251 750333 504334 504353 ACAGAGTGTTTACTGTGAGC 29252 750334 504503 504522 TCCATGGAATGGCTGTCATG 39 253 750335 504731504750 TTCCTTCAGAGTTATTTCTT 73 254 750336 504740 504759TTATTCTCCTTCCTTCAGAG 70 255 750337 504741 504760 GTTATTCTCCTTCCTTCAGA 41256 750338 504817 504836 GAAGTAGTCCTGCCCTTTCC 60 257 750339 504923504942 ATATCCTTTCCTCTCCTACT 78 258 750340 504958 504977GACTGATGGTTACCCATACA 40 259 750341 504987 505006 CATTTAACAACTATTATCTT 27260 750342 505003 505022 TCATTAAATAGCTAACCATT 91 261 750343 505043505062 CCACTTTGCAACTCAAGATT 31 262 750344 505046 505065CAGCCACTTTGCAACTCAAG 20 263 750345 505051 505070 GACTCCAGCCACTTTGCAAC 62264 750346 505073 505092 CCAGAAATTTAGTCTGTTGT 59 265 750347 505096505115 CATAAGCTGCTGGATTTGTT 51 266 750348 505241 505260GAAGAGAACGAGGATATAAA 35 267 750349 505306 505325 GTGATATATTAGAACTGTAT 28268 750350 505443 505462 AGAGGATTTAGAGTTAAAAT 22 269 750351 505569505588 ACTGAAAGGTCTGTATGTTT 73 270 750352 505656 505675CAGGCCTAGCTTCCACCTAA 108 271 750353 505837 505856 TCTGTCAAAGACCTGTGAGG56 272 750354 505922 505941 TCTGCTTGTGGTTCTTCCCT 71 273 750355 505985506004 CAAATATGGAATAGCACTTG 54 274 750356 506040 506059GTTGAGAACGGTATTGAGTA 56 275 750357 506052 506071 CTCTTGTTTTCAGTTGAGAA 42276 750358 506097 506116 ATTTCTCCATGGACTCCAGA 47 277 750359 506109506128 CATTGGCTTCATATTTCTCC 11 278 750360 506306 506325TATTGCCTTCACTGCTGCCT 9 279 750361 506518 506537 ACTGACTTGCTCTGCCTACT 71280 750362 506617 506636 GAGAAGTCTCTCATGGCACC 40 281 750363 506731506750 GGAGTGCTCCACACTTCTGT 27 282 750364 506776 506795CTCCAGGTTGTGGAGGTTGT 75 283 750365 506783 506802 GTATATACTCCAGGTTGTGG 23284 750366 506881 506900 ACTTCTCATTCTCTCCACCA 17 285 750367 507189507208 AAAGCATTTTCTACCAGAGC 45 286 750368 507198 507217TAGACAAGGAAAGCATTTTC 54 287 750369 507312 507331 CACCTGCCCTGCTTTTGCTT 41288 750370 507324 507343 TGGTCCTAGCTTCACCTGCC 89 289 750371 507357507376 AGATCCAACCTGTGTGGAAA 61 290 750372 507534 507553AGAAATTAGAGCCAGGTTCC 36 291 750373 507635 507654 TCCCCCCAGAAGGCTTGACT 71292 750374 507755 507774 TCGCTGGCCATTTCATATAT 44 293 750375 507978507997 AAAACATGTTAACTGTTATC 40 294 750376 508159 508178TTAGCCATGTGCTTTGTGAC 68 295 750377 508208 508227 TCCCTTCTGTCATCTGATCT 59296 750378 508218 508237 AAGGTAGTTCTCCCTTCTGT 93 297 750379 508426508445 TTCCATTGCACTCCTTTCTA 113 298 750380 508530 508549TAAAAGGAAAACCCACTTGT 72 299 750381 508700 508719 TACAGAAATTACCAGTAAAG 38300 750382 508710 508729 TCCAGATTTCTACAGAAATT 102 301 750383 503980503999 TCACTGCACACTGTACAGGA 78 302 508746 508765 750384 508890 508909TGCCTAGTTCCCTCCTGAAA 98 303 750385 508908 508927 TTATGTTTACTTCATGACTG 91304 750386 508945 508964 CTTCATTATTCTCTAGTGCC 14 305 750387 509013509032 CTATTCCCCTTCAACATGTG 44 306 750388 509060 509079CATACCCAACATGCTTGCAT 44 307 750389 509079 509098 AATGCATTACCCTACAATGC105 308 750390 509127 509146 CTCATCACAACTGGGTGGTA 76 309 750391 509134509153 ATCCCAGCTCATCACAACTG 88 310 750392 509167 509186TCCAAGTGCTTCAAGCTGAG 100 311 750393 509189 509208 CACCCACAGCAGGCAGATAA58 312 750394 509208 509227 TACCTTGCTCCAAAATAATC 37 313 750395 509230509249 CTGTTTCTTGAGGCAAATGA 54 314 750396 509787 509806CTTTTCTCAATGTCAACTCT 48 315 750397 510028 510047 AGAGTTATACACGGAACCCA 71316

TABLE 6 Reduction of UBE3A-ATS RNA by 8,000 nM 5-10-5 MOE gapmers withmixed PO/PS internucleoside linkages in vitro (40,000 cells/well) SEQ IDSEQ ID UBE3A- Compound NO: 1 NO: 1 ATS SEQ ID Number Start SiteStop Site Sequence (5′ to 3′) (% UTC) NO 617456 463905 463924CAACTGTTACCAAGACTTCA 26 32 617457 465003 465022 ATCTGCGAATAGAGGCCCTC 4633 617460 466539 466558 TAGGTTGCATAAAGCCAGGC 8 36 617461 466981 467000CACACATCTTGTTCCCTCAA 21 37 617557 483977 483996 ATCATGTGCATACCCAGGGT 23172 749968 465163 465182 TTCTAGGGCTCCAGTTTATG 86 317 749969 465238465257 TGGTCATCTCGGGTATATAA 20 318 749970 465341 465360CTTACTATCTTCAAGAATTC 68 319 749971 465346 465365 TTTTTCTTACTATCTTCAAG 56320 749972 465394 465413 AGTCAATTGTCAGACTTATT 37 321 749973 465587465606 AAGGAGTTCCTTTAGCATTT 27 322 749974 465594 465613CAGACCGAAGGAGTTCCTTT 50 323 749975 465612 465631 TCCAGTGCCTTTCTATTTCA 33324 749976 465686 465705 ACTTTGTTTTAAACTTACAC 46 325 749977 465765465784 CATCAACACAAGTTTATAAT 47 326 749978 465892 465911AACTCCCACAAGGTACTCTT 32 327 749979 465906 465925 ACTGTCAACTCCTGAACTCC 54328 749980 465956 465975 CAATGAATCTATTCTTAGAT 122 329 749981 465978465997 GTGAAACTGTTTATACCCTT 25 330 749982 465996 466015GTTGGTGATGCAGGTAAAGT 24 331 749983 466024 466043 TTGTTACTGAGCTGTGCCAC 48332 749984 466119 466138 AAGTGGATCTCTTGGGCAGG 22 333 749985 466182466201 TTTACCCTCTCCCAACCACT 51 334 749986 466217 466236GGCTGCTGTTTGAGTCCCCA 40 335 749987 466220 466239 ATGGGCTGCTGTTTGAGTCC 58336 749988 466221 466240 TATGGGCTGCTGTTTGAGTC 55 337 749989 466529466548 AAAGCCAGGCCAGGTGCTGA 64 338 749990 466532 466551CATAAAGCCAGGCCAGGTGC 30 339 749991 466534 466553 TGCATAAAGCCAGGCCAGGT 20340 749992 466537 466556 GGTTGCATAAAGCCAGGCCA 36 341 749993 466539466558 TAGGTTGCATAAAGCCAGGC 46 36 749994 466541 466560TCTAGGTTGCATAAAGCCAG 52 342 749995 466544 466563 TTCTCTAGGTTGCATAAAGC 33343 749996 466546 466565 CCTTCTCTAGGTTGCATAAA 28 344 749997 466549466568 TCACCTTCTCTAGGTTGCAT 34 345 749998 466551 466570TATCACCTTCTCTAGGTTGC 44 346 749999 466701 466720 AAATAACTGGATCTCATAAC 55114 750000 466979 466998 CACATCTTGTTCCCTCAAGG 26 347 750001 466981467000 CACACATCTTGTTCCCTCAA 26 37 750002 466983 467002TTCACACATCTTGTTCCCTC 26 348 750003 466986 467005 TGCTTCACACATCTTGTTCC 43349 750004 466988 467007 CCTGCTTCACACATCTTGTT 49 350 750005 466991467010 GAACCTGCTTCACACATCTT 36 351 750006 467049 467068CTTTCATCAGTTAGTCAGGT 23 352 750007 467200 467219 GGAGTTGGTTATTGGAAAAT 48353 750008 467289 467308 TCTATTGGTGTTCCTTTTAG 48 354 750009 467296467315 AGAGTAGTCTATTGGTGTTC 22 355 750010 467363 467382CTTTTAAGATAATTTTTCTC 90 356 750011 467417 467436 TACGCTCCTTCATTTCATGC 55357 750012 467506 467525 TTTTTAATCCTCTGATGAAT 102 358 750013 467508467527 TTTTTTTAATCCTCTGATGA 62 38 750014 467604 467623CTCTCTTCTCCTTTATGACT 41 359 750015 467628 467647 CTTAAATAAGTTTTCTACCC 42360 750016 467639 467658 AGCCATTATTTCTTAAATAA 56 361 750017 467669467688 CATATCTTTTCCTAGATTTG 110 362 750018 467813 467832AGAATCCTGTCTCCCTCTTA 34 363 750019 467916 467935 TGAGTCTCTTTTCTTTCGGT 41364 750020 467919 467938 TGATGAGTCTCTTTTCTTTC 35 365 750021 467921467940 TGTGATGAGTCTCTTTTCTT 30 366 750022 467962 467981TACTGAGAAATCTCTTGAAT 77 367 750023 468277 468296 TAAAATTATTTATACACCAT 73368 750024 468359 468378 TTTATACTGTAGTATGCATT 53 369 750025 468410468429 TTACTTCCCTACCCTTGCAT 75 370 750026 468413 468432CTTTTACTTCCCTACCCTTG 67 371 750027 468464 468483 ATTTATTTTAAGCTGATAAC 52372 750028 468733 468752 AAATCCATTTGTCCAGTCTG 14 373 750029 468888468907 TATCTGCATGCAATATCTTT 70 374 750030 468924 468943CTGTAAGTATAGATGCCTCT 15 375 750031 468968 468987 TTAGCCTTTTGATATAGTTT 25376 750032 468988 469007 GCTTCACCATTTTGACCTTC 19 377 750033 469140469159 TTTTGAAAGGGAGGCACTGA 27 378 750034 469604 469623TGAGGTGTAATGTTGTTTAT 32 379 750035 469717 469736 TAATAGTCTCTATTGTTTTT 50380 750036 469884 469903 CAAGTTAACTAATATGTTGG 71 381 750037 469985470004 TATTGATTCAATTCCCTTAT 23 382 750038 469988 470007GAATATTGATTCAATTCCCT 37 383 750039 470062 470081 AATTTTTTTAAATGGTTGGC 51384

TABLE 7 Reduction of UBE3A-ATS RNA by 8,000 nM 5-10-5 MOE gapmers withmixed PO/PS internucleoside linkages in vitro (40,000 cells/well) SEQ IDSEQ ID UBE3A- Compound NO: 1 NO: 1 ATS SEQ ID Number Start SiteStop Site Sequence (5′ to 3′) (% UTC) NO 617456 463905 463924CAACTGTTACCAAGACTTCA 18 32 617457 465003 465022 ATCTGCGAATAGAGGCCCTC 3633 617460 466539 466558 TAGGTTGCATAAAGCCAGGC 12 36 617461 466981 467000CACACATCTTGTTCCCTCAA 16 37 617557 483977 483996 ATCATGTGCATACCCAGGGT 27172 750182 488205 488224 AACAGAGAATTGTATATCCA 43 385 750183 488322488341 ACATGAGATCTTTAATAAGA 73 386 750184 488343 488362CAAGAGAGAAGCCACTCATG 64 387 750185 488685 488704 AGCAGATTTGAGCTGGAAGA 42388 750186 488928 488947 AACTGGCAGAAAATATCTCT 36 389 750187 488941488960 AAAGAAGAACATAAACTGGC 70 390 750188 489021 489040GTTGGCTCTATTTTCAAATG 33 391 750189 489449 489468 CTGTTTAGGCTGGACACTGT 90392 750190 489478 489497 ATTCAGGAAATTAAAGCATT 60 393 750191 489698489717 AGCAGAACAGACCTTATTTG 41 394 750192 489712 489731CCTTCAACCAGAGGAGCAGA 44 395 750193 489756 489775 GATCTTGGTTTGTAGAAGGT 35396 750194 489824 489843 TTCAAAATGGTAGAAAATTG 64 397 750195 489873489892 TGAAAGGCCTACAGCAACCA 46 398 750196 489893 489912ATGGGAGCTCTGGAAAACAG 27 399 750197 490070 490089 TTCTGAGTATATGTGAAACA 39400 750198 492874 492893 ATAAGGAGATTAATTTAAGA 86 401 750199 493278493297 GAATCAAAGAAAGAAGGAAT 47 402 750200 493440 493459GCTGTAATAATAATCATATT 41 403 750201 493678 493697 AGAATTCTTCCCTACAGGTT 51404 750202 493831 493850 CAGTAATTAATGTTCTTATA 37 405 750203 493839493858 CCCCAATTCAGTAATTAATG 39 406 750204 493841 493860GGCCCCAATTCAGTAATTAA 56 407 750205 493882 493901 ATGTAACCTATTCAAGATGA 47408 750206 493908 493927 TTATTTGGAAGAAGAATCTT 98 409 750207 494002494021 TATAAAATACTTTTTATGGG 101 410 750208 494398 494417GACTGTGATAAAGATGTATA 84 411 750209 494443 494462 TAAGGGTCATGTACTATACA 33412 750210 494494 494513 GTGTGTGCAATAGCCTAAAT 27 413 750211 494554494573 CAGAAGCAAAAGATAGCAGC 53 414 750212 494873 494892TATTAATGTACTTGAAGATG 52 415 750213 495043 495062 ATCAGTGTGTGCAGATTCTG 45416 750214 495050 495069 TCCCATCATCAGTGTGTGCA 27 417 750215 495201495220 TATGAAAATTAATGTTCAAG 80 418 750216 495233 495252AAAAAATTTTAAACCCCTAG 76 419 750217 495441 495460 TCATGAAAGTAGAGAGTAAG 68420 750218 495659 495678 TGAAACAATTTAAGTGCCCA 64 421 750219 495718495737 TTGAAGGGATATCTTCATTC 106 422 750220 495795 495814TCTGAAAAAAATAGAACAAC 64 423 750221 496202 496221 AATAAAAGACCTGCACAGCA 66424 750222 496205 496224 CAAAATAAAAGACCTGCACA 48 425 750223 496379496398 AGACCCTTATTTACACCATA 65 426 750224 496557 496576CTATTAATGAGCTATCTGAA 37 427 750225 496561 496580 TCTACTATTAATGAGCTATC 45428 750226 496567 496586 TTTCCATCTACTATTAATGA 39 429 750227 496661496680 GTCTCCACCAGAACAAAACT 69 430 750228 496691 496710ATGACATAACCATATACAAA 35 431 750229 496697 496716 TTACTGATGACATAACCATA 71432 750230 496869 496888 CAAAATTCAACCTGGTTTCA 39 433 750231 496927496946 ATGGAGGGTGTAAATCAAAT 36 434 750232 497148 497167TGTTACACTGTTATTAAAGC 28 435 750233 497197 497216 AAAACAATGAAGCAGAGGGA 37436 750234 497299 497318 TAATAAAAAGTATCCCACCA 72 437 750235 497429497448 GAAGTTATGCACCATCAAAT 46 438 750236 497477 497496TTTACTACTGATATCACCAA 41 439 750237 497680 497699 AGAACAGACTAAGCCCGAAA 40440 750238 497917 497936 AATTCTGGTGGAAAATACAG 71 441 750239 497984498003 ACAGAAATCATATCAAATCC 69 442 750240 498096 498115ATATATGAAACATTCCATCC 39 443 750241 498127 498146 TTACACTTTAGACCAAACGG 37444 750242 498179 498198 GGGCAGATTGATCACCTAGA 25 445 750243 498208498227 CGGGACCTCAATACTCTACT 37 446 750244 498432 498451TAAATGGAAATTGAGAGCAG 61 447 750245 498842 498861 AAGTCACAACCACATACTGC 57448 750246 498894 498913 GATTCTCCAACAAAAAGACA 50 449 750247 498942498961 GACACTAGCAGTTTCTTACC 30 450 750248 499132 499151GCTTAAAGTAGCCTAAGGAT 32 451 750249 499286 499305 ACAATAAGTTGTAGCTACAA 61452 750250 499304 499323 TTAAAAACAACTGTAGCTAC 62 453 750251 499335499354 TTCCTATTAGGAGGTTTAAA 43 454 750252 499395 499414ATAAGTAATTCATAGTCAGA 44 455 750253 499452 499471 GGCTTAAATAAAAGACTGCT 63456

TABLE 8 Reduction of UBE3A-ATS RNA by 8,000 nM 5-10-5 MOE gapmers withmixed PO/PS internucleoside linkages in vitro (35,000 cells/well) SEQ IDSEQ ID UBE3A- Compound NO: 1 NO: 1 ATS SEQ ID Number Start SiteStop Site Sequence (5′ to 3′) (% UTC) NO 617456 463905 463924CAACTGTTACCAAGACTTCA 34 32 617457 465003 465022 ATCTGCGAATAGAGGCCCTC 4433 617460 466539 466558 TAGGTTGCATAAAGCCAGGC 45 36 617461 466981 467000CACACATCTTGTTCCCTCAA 19 37 617557 483977 483996 ATCATGTGCATACCCAGGGT 37172 749753 448285 448304 TAAGATTCCATTGCCAGAAT 115 457 749754 448296448315 AAGTTTCGCAATAAGATTCC 103 458 749755 448500 448519TTTCAGTCAGCAAAGGCAGC 80 459 749756 448681 448700 TCTCAACCCTGAAAACAATC 87460 749757 448989 449008 CATTGGCATTATTCACAGCA 103 461 749758 449031449050 CTGACTGTCATTCATATAAG 69 462 749759 449067 449086TGCATAACGATAATCATGTG 161 463 749760 449112 449131 TTGTAGATCCTGGCAAGTAT132 464 749761 449130 449149 AAAAATAAAGTTTCCCTCTT 64 465 749762 449167449186 CAAAACTTGTCAACATTATA 129 466 749763 449218 449237CTATATTTAGACCAAATGAG 91 467 749764 449254 449273 CTGACATATATAAATTAGAA104 468 749765 449263 449282 CATAGCAACCTGACATATAT 109 469 749766 449279449298 ACAAACATTGTAAAAACATA 92 470 749767 449337 449356TTAGAACATGTAGCCATAAT 69 471 749768 449382 449401 AATTGTATTTAATTTAATGA102 472 749769 449439 449458 ATCATACTGGAGCCAGGTGA 85 473 749770 449545449564 AATGCAGAAATAAGACCTTC 88 474 749771 449765 449784CAGTCATCCTTATCTAAGGG 63 475 749772 449786 449805 AAGCCAAAGAGTACTCTTCC 83476 749773 449820 449839 GATATCTGGGTGTGTCAGCT 67 477 749774 449944449963 CAACATAAGGTCTTATTGTT 74 478 749775 450083 450102GGTCTTCTAGAAGCTAATAG 77 479 749776 450092 450111 TTGATAAGTGGTCTTCTAGA 64480 749777 450204 450223 CAATTCTTTTGAGTGTGTAC 66 481 749778 450205450224 TCAATTCTTTTGAGTGTGTA 70 482 749779 450511 450530GTGTGACATTGTCATCATAC 87 483 749780 450710 450729 CTTTATGCTTTCTGTTCTTT 76484 749781 450733 450752 GAGACTCTCTTTGTTTCTTT 96 485 749782 450906450925 TTCATTGGAATTCATTGAGC 94 486 749783 450978 450997CAAGACTTTCTTCTTGTTTT 115 487 749784 451042 451061 AGTCAGCTGTTAATCTTCCT85 488 749785 451104 451123 TTTTTCTTTGAGCATTTTTA 104 489 749786 451250451269 TTTAAGTATTTAGAGAACTC 122 490 749787 451286 451305TACCTAGGCTCACTTGCTTT 91 491 749788 451306 451325 TAGTTGTTGATTTGAATAAC 87492 749789 451308 451327 TGTAGTTGTTGATTTGAATA 93 493 749790 451389451408 TTATTTTAAAATCACTTCAG 96 494 749791 451466 451485TCTTCTTTGGCAAATATATT 126 495 749792 451604 451623 TCCCTTTTTGCCATCTTTTG58 496 749793 451673 451692 AATAAGTTTCTAATTTACAC 113 497 749794 451834451853 TCTCATGTGTTTTTGTTCCT 49 498 749795 451954 451973TAAACCTGGAGATTTTATCC 128 499 749796 452167 452186 GGGAACACACCATTCAGCAG38 500 749797 452222 452241 AATCTTGTTGACTAAAAGTA 114 501 749798 452255452274 GTCTCACGCTGTGTGAATCA 104 502 749799 452257 452276AGGTCTCACGCTGTGTGAAT 89 503 749800 452285 452304 TGCTCCTATTTCAATATGAG100 504 749801 452324 452343 ATAAGGTTCAAAAGCCGGTG 182 505 749802 452459452478 AACTGGTAACATAAATGCAG 99 506 749803 452485 452504TCAGAGCAAAATGGACTGAC 142 507 749804 452517 452536 TACAAATACCCAGTCTGCAG65 508 749805 452590 452609 AAGGCCCTTATCATATGCCA 96 509 749806 452661452680 TCCTATAAAAGAAGATATTT 65 510 749807 452715 452734CTGTGAGAGCTCTGCCCTCA 93 511 749808 453217 453236 GTTAATAGTGTTCTTACATC106 512 749809 453233 453252 CAGTGGAGTCAAGTTGGTTA 135 513 749810 453350453369 TCTACTAGCTAACTTTATCA 109 514 749811 453453 453472AGGATCAAAGCAGAAATTAA 102 515 749812 453814 453833 AAAAACATAGACTTTACTAA139 516 749813 453899 453918 CACAAAAAACTGATTATATA 92 517 749814 453933453952 GATCTGTGTTGTTCTTAAGT 73 518 749815 453978 453997AAGGTAGATTTTATGGCTAC 68 519 749816 454002 454021 GGGCAAAAATAGCAACATAA 47520 749817 454067 454086 AAGACATAGATGATCCCATA 89 521 749818 454085454104 TATGAGATGTAAGGAGACAA 86 522 749819 454303 454322GATAGGAGTAATCTTTTTTT 86 523 749820 454611 454630 AAACAAAAACTCTCCTAAGT 77524 749821 454657 454676 AGAACTAACAGAATTAAGAG 89 525 749822 454658454677 CAGAACTAACAGAATTAAGA 111 526 749823 454661 454680ACACAGAACTAACAGAATTA 71 527 749824 454759 454778 AAAAAGAGGAAAACCGAAAG 88528

TABLE 9 Reduction of UBE3A-ATS RNA by 8,000 nM 5-10-5 MOE gapmers witha mixed PO/PS internucleoside linkages in vitro (35,000 cells/well)SEQ ID SEQ ID UBE3A- Compound NO: 1 NO: 1 ATS SEQ ID Number Start SiteStop Site Sequence (5′ to 3′) (% UTC) NO 617456 463905 463924CAACTGTTACCAAGACTTCA 40 32 617457 465003 465022 ATCTGCGAATAGAGGCCCTC 4933 617460 466539 466558 TAGGTTGCATAAAGCCAGGC 28 36 617461 466981 467000CACACATCTTGTTCCCTCAA 33 37 617557 483977 483996 ATCATGTGCATACCCAGGGT 42172 749825 454828 454847 CATACAAATGTCACTAATAT 114  529 749826 454836454855 AAGACTAGCATACAAATGTC 85 530 749827 454864 454883ACAGGTGTATATTCCCTATC 84 531 749828 454998 455017 ATATATCTACTGAATATGAT108  532 749829 455062 455081 CTCAGAACCCTTTGGTCTAA 66 533 749830 455110455129 CTGACTTGACTTTAATGAAA 68 534 749831 455148 455167GCATTCAGCTTATAGCAGAA 58 535 749832 455150 455169 CTGCATTCAGCTTATAGCAG 84536 749833 455193 455212 AAACATTCCTCATACCACAC 73 537 749834 455230455249 CTTATCCTTGATCCAGACAA 78 538 749835 455436 455455AATGGGAAATGCTAAAGTTG 72 539 749836 455459 455478 AGTGACACAGTAGTTGTATC 70540 749837 455485 455504 CAATTTCCTCTCTACCAAGC 71 541 749838 455542455561 CTTTGTCATTTCATTTATAA 70 542 749839 455603 455622ATACAGGCATCTCAGCCCTC 61 543 749840 455767 455786 AAAGTACCAAAGTGGCTGCT 71544 749841 455768 455787 AAAAGTACCAAAGTGGCTGC 68 545 749842 455829455848 GAAATAATGAACTCACAGTC 70 546 749843 455960 455979CACAGTTGTTTAGGTCATCT 119  547 749844 457070 457089 GCATGCCATATTTCCTTCTT91 548 749845 457076 457095 ATAGAGGCATGCCATATTTC 86 549 749846 457151457170 AGCAAAAATAATCTTAGAAA 91 550 749847 457194 457213GATTCCAGGCCTTCTATCTC 80 551 749848 457354 457373 GGTCTCAATGAGGAAAAGGA 34552 749849 457424 457443 ATAAAAGCAAAAGAGAGTTA 84 553 749850 457556457575 TCTATTCTGAAACCCCAATA 45 554 749851 457705 457724GACATGCCATCAAGAGAAGA 59 555 749852 457917 457936 ATGTATTAACCAATATTTTG 51556 749853 457993 458012 AGAACCCACTTGATCTATTA 53 557 749854 458199458218 TAAAGAATTGAGTACCAAAA 64 558 749855 458268 458287TATCACATTAATTCCCTCTC 37 559 749856 458289 458308 TAGAATAGAAAAGCATGAAG 71560 749857 458328 458347 AACAACTATATTTGCTTGTA 39 561 749858 458346458365 ATAATCACACTAAATCTTAA 70 562 749859 458391 458410CCTCTATTAAGATCTATGAG 75 563 749860 458438 458457 ACTTCATCAATATTTCCCCA 31564 749861 458537 458556 GCACTTAAATTTATCAGTTG 28 565 749862 458539458558 AAGCACTTAAATTTATCAGT 36 566 749863 458567 458586TTAGTATGTCGAGAACTCAA 24 567 749864 458588 458607 AAGTTGAAACACATTTTAGC 32568 749865 458596 458615 AGGATTAAAAGTTGAAACAC 30 569 749866 458597458616 CAGGATTAAAAGTTGAAACA 49 570 749867 458608 458627AATCAGGGAAGCAGGATTAA 55 571 749868 458668 458687 TGATTACTCTTGGCAGTAAT 58572 749869 458823 458842 TGATAGATACTTGTATTAGC 14 573 749870 458896458915 CAAATCTAAAGCTCATTTAC 72 574 749871 458908 458927CTACCAAAATGTCAAATCTA 56 575 749872 459220 459239 GTAACCTGAATATTTCATGA 37576 749873 459228 459247 AAATAATGGTAACCTGAATA 56 577 749874 459240459259 AATACATTACTGAAATAATG 86 578 749875 459256 459275TTTACCTTGACATTCTAATA 40 579 749876 459450 459469 TTAACCTATTTTAATAATAT 89580 749877 459567 459586 TTAAGTGATTGGAAATAAAA 82 581 749878 459580459599 CAATTAGGAATATTTAAGTG 97 582 749879 459633 459652CCAGGCAATGGCTCTTTCAA 66 583 749880 459645 459664 TGTATAGTTTACCCAGGCAA 37584 749881 460066 460085 CTTTATCAATCTAATCAATT 65 585 749882 460115460134 ACTTTATAGTGTGGATGGTA 22 586 749883 460146 460165TTTTAGGGAATTGTCCTGAT 39 587 749884 460211 460230 AAGGAAACACACATAATACC 46588 749885 460213 460232 GGAAGGAAACACACATAATA 26 589 749886 460272460291 CACTAAGGACAAAGATATGG 75 590 749887 460303 460322TATTTGTTCATTCTCAAGAA 56 591 749888 460545 460564 CAACCTGGGCTCTCATCTAA 41592 749889 460709 460728 AATGCTTGATCTGTGGGCTC  32* 593 749890 460749460768 ATTGTGTACACAGGGATTTG  22* 594 749891 460792 460811AACTGTATACTTTGAAAGTA 68 595 749892 460870 460889 GAAGCAAGTAAGTAAATAAT 81596 749893 460981 461000 CTTAGATGTGTTTATCCAAA 25 597 749894 460999461018 GTGTTTTTCCATTTTTCTCT  9 598 749895 461027 461046TGGCTCTATCAAGGCTTCCC 39 599 749896 461039 461058 AATCCTATATTTTGGCTCTA 34600

TABLE 10 Reduction of UBE3A-ATS RNA by 10,000 nM 5-10-5 MOE gapmers withmixed PO/PS internucleoside linkages in vitro (40,000 cells/well) SEQ IDSEQ ID UBE3A- Compound NO: 1 NO: 1 ATS SEQ ID Number Start SiteStop Site Sequence (5′ to 3′) (% UTC) NO 617456 463905 463924CAACTGTTACCAAGACTTCA 47 32 617457 465003 465022 ATCTGCGAATAGAGGCCCTC 7033 617460 466539 466558 TAGGTTGCATAAAGCCAGGC 51 36 617461 466981 467000CACACATCTTGTTCCCTCAA 45 37 617557 483977 483996 ATCATGTGCATACCCAGGGT 29172 699780 479654 479673 AGTAAGGTCTGTTATTCTCC 122 127 750040 470321470340 GAACTATCCTGCATCCGAGG 41 601 750041 470398 470417CAACCATCGAGATGATCATA 70 602 750042 471812 471831 TAAATGCTTTTCTAAATCTA100 603 750043 471892 471911 GCTTTTATAGTGTTGAGGCA 81 604 750044 472153472172 TTAAGCTCTAATTAAAACAG 113 605 750045 473241 473260TATTTCACTGGAGCTTTGAT 107 606 750046 473564 473583 AATCATCTACTGATGAACAC99 607 750047 473573 473592 TCTTTATCTAATCATCTACT 90 608 750048 473576473595 TTCTCTTTATCTAATCATCT 111 609 750049 473697 473716AGTTCCTGGAAACCGCCATT 63 610 750050 473789 473808 ATGCTGTTGTACACTAGATC 82611 750051 473955 473974 GTACACTATTGTTTTGATAT 44 612 750052 474053474072 CACCCCTAATTTATATTACT 56 613 750053 474069 474088CATAGGTCAATTCCTTCACC 47 614 750054 474103 474122 TTAATTTAAATAGTTTACAA100 615 750055 474123 474142 CTAGCTTGAATGGATACCAA 71 616 750056 474169474188 TAGTGGTTGCCTTAGTATTA 52 617 750057 474198 474217CAAGTGCTATATTTTTTTAA 95 618 750058 474230 474249 TATATATAATTGAGGGCCAC116 619 750059 474414 474433 ATAATTATAAGATAGGGTTT 127 620 750060 474618474637 ATTAGTATTGCTGCTCTAGC 91 621 750061 474786 474805CCAACTGTAATCATTGATTT 79 622 750062 474865 474884 GATAATGTGTAGTTTTTTAT 82623 750063 474878 474897 TAAGGTGTTGTATGATAATG 78 624 750064 474953474972 AATATGTGTCATCCTGAAGA 93 625 750065 475066 475085TCATTGTGAATTTCCCACAT 81 626 750066 475233 475252 TTAGGGATATACTGTTATAC 72627 750067 475268 475287 AAAATTAGGTTATTGGATTG 103 628 750068 475293475312 CTTTCACTCTCATTTCTTAA 91 629 750069 475397 475416ACTTTCAAGTTTATTAATTT 115 630 750070 475495 475514 TACTTTCATTTATGTCTAGT78 631 750071 475503 475522 GATATCTATACTTTCATTTA 125 632 750072 475628475647 CATGTTTTTACACCAGCTGT 87 633 750073 475693 475712TTACTTACTCAATTTCTTCT 97 634 750074 476072 476091 GTTTCAGCAGTTTCTGCTCC 85635 750075 476164 476183 GGGCACTTAGGAGTTCCTAA 80 636 750076 476337476356 CTCTGAGAGTGTTTAGAAAT 55 637 750077 476350 476369GTGAAAGAAAATGCTCTGAG 62 638 750078 476405 476424 TACATCAGACAAGGCTCAGG 91639 750079 476845 476864 TTCTATAATCTTATGGTTAA 77 640 750080 476851476870 GAAGTGTTCTATAATCTTAT 74 641 750081 477296 477315GTGAAAATTTTGTAGGTTGC 110 642 750082 478347 478366 TACAATCAGTGTCTTTCACC78 643 750083 478472 478491 ATGGTTAGGGCTATGTTATG 96 644 750084 478542478561 GAAATAAAACGCAATGTATC 118 645 750085 478710 478729CTTGCATGTTGCCTTTTTCT 129 646 750086 479037 479056 AACTCTCTGCCATTATTACT81 647 750087 479330 479349 TAATCTCACTGGATACAGAA 107 648 750088 479468479487 AAACATTTTACCATTTTATA 133 649 750089 479644 479663GTTATTCTCCCTCTTGAACC 82 650 750090 479647 479666 TCTGTTATTCTCCCTCTTGA 86651 750091 479649 479668 GGTCTGTTATTCTCCCTCTT 51 652 750092 479652479671 TAAGGTCTGTTATTCTCCCT 52 653 750093 479656 479675AAAGTAAGGTCTGTTATTCT 92 654 750094 479659 479678 CTAAAAGTAAGGTCTGTTAT 74655 750095 479661 479680 GACTAAAAGTAAGGTCTGTT 70 656 750096 479664479683 AATGACTAAAAGTAAGGTCT 133 657 750097 480219 480238AAAGATATAGAACTGAAAAG 128 658 750098 480286 480305 GTTGGAGACTTCAATTTCCT89 659 750099 480361 480380 AAACAACAGAGCCTCAAATA 114 660 750100 480441480460 AAAATACCACAAAGATAGGC 38 661 750101 480530 480549AAGAATGGAAAAGATGTATC 57 662 750102 480533 480552 TAAAAGAATGGAAAAGATGT 99663 750103 480648 480667 AATTTTACCTCAGTATAAAA 106 664 750104 480698480717 ATTACCAAATGCCACTGTAT 59 665 750105 480714 480733GTTGCATAACATGTGTATTA 65 666 750106 480787 480806 AATTAACTGCTTAATGAGTA145 667 750107 480898 480917 ATAAAGGTCACTTATTGTAT 89 668 750108 480968480987 AATAAAACTGATACATACTA 101 669 750109 481010 481029AATCAACTTTTAGCTATACA 119 670 750110 481027 481046 TATCATTATGACCTAGGAAT69 671

TABLE 11 Reduction of UBE3A-ATS RNA by 8,000 nM 5-10-5 MOE gapmers withmixed PO/PS internucleoside linkages in vitro (40,000 cells/well) SEQ IDSEQ ID UBE3A- Compound NO: 1 NO: 1 ATS SEQ ID Number Start SiteStop Site Sequence (5′ to 3′) (% UTC) NO 617456 463905 463924CAACTGTTACCAAGACTTCA 16 32 617457 465003 465022 ATCTGCGAATAGAGGCCCTC 2133 617460 466539 466558 TAGGTTGCATAAAGCCAGGC 24 36 617461 466981 467000CACACATCTTGTTCCCTCAA 15 37 617557 483977 483996 ATCATGTGCATACCCAGGGT 12172 750254 499480 499499 ACTGGAATTACTAAAAGGGA 62 672 750255 499621499640 AGTAGAGAGTGGGATGGTAT 53 673 750256 499691 499710TAAGAAACATCACATTCAAG 67 674 750257 500139 500158 TGAACAGAATGAGAAGTTTA 82675 750258 500141 500160 TCTGAACAGAATGAGAAGTT 69 676 750259 500285500304 TGGTGTACATTGGATATGAA 24 677 750260 500319 500338TTTCTAACATTTACTGTGGA 83 678 750261 500367 500386 TTCTAACATACTAATTAGCA 41679 750262 500384 500403 CACCAATGCAAGCCAGCTTC 28 680 750263 500526500545 TGTTTAGAGGTCAAGCCCTG 75 681 750264 500570 500589ATCAGTCAAAACATGTTCTG 56 682 750265 500629 500648 TATTACACAAGGTATTGGTA 35683 750266 500639 500658 ATGAAAGGTTTATTACACAA 34 684 750267 500760500779 AATCAATTTGTGCCACAGGC 34 685 750268 500786 500805GCAGCTTATAAAGAGAGCCA 78 686 750269 500860 500879 TTTGGTAGGTAACTACGGGT 35687 750270 500882 500901 TCGGATATAGCTTTTACATA 14 688 750271 501004501023 AAACTAAGCACATCCGATAG 60 689 750272 501060 501079ATCATCTTTGATTTGACTTT 37 690 750273 501091 501110 TTCTTTATGAATCTTTGAAA 51691 750274 501151 501170 GTAAAGAGCCACCTAAGGGA 40 692 750275 501165501184 ATGAGATGGGCACAGTAAAG 33 693 750276 501391 501410TCCCCAGATAATGCATAGAT 49 694 750277 501437 501456 GGTGGCATAGAAGGCAGCAC 74695 750278 501549 501568 GGATGCAGCAGGAGAAGAAA 42 696 750279 501576501595 AAGGCAAGGCTAAGGAGTGC 27 697 750280 501679 501698AGAGAGCTAGAGCTAGGACT 17 698 750281 501694 501713 ATCCCAAAGTTACACAGAGA 58699 750282 501703 501722 CTTTGGGACATCCCAAAGTT 73 75 750283 501752 501771CACCTGTATCCAAAATTCAA 47 700 750284 501788 501807 CATATCTGAAGCACAGAGAG 39701 750285 501860 501879 CAGTCTGCTCTGCTGCTCTG 66 702 750286 501862501881 TCCAGTCTGCTCTGCTGCTC 67 703 750287 501882 501901AGCTCAGAGGCAGCAGGAGC 47 704 750288 502030 502049 CTAAGCTCCATATTTAAATC 69705 750289 502049 502068 TTCAGGCTTCCTTCACAGCC 69 706 750290 502052502071 TTCTTCAGGCTTCCTTCACA 44 707 750291 502138 502157GGAATCAGTGCTACCCATTA 23 708 750292 502194 502213 TTAGCCATCATTTTATTCTC 12709 750293 502341 502360 GGCCCATTTTTTCAATCTCA 43 710 750294 502404502423 CATGGTCTTCCTTGACTACA 40 711 750295 502580 502599AAGCCAATGCGCAAGAAAAG 42 712 750296 503121 503140 CATCCAGTTAATCTCTGACA 31713 750297 503125 503144 CTCGCATCCAGTTAATCTCT 38 714 750298 503145503164 ACACCATCCCAGAAATGGTC 75 715 750299 503180 503199TTTCTGACTCCCTATCCAGT 44 716 750300 503376 503395 TTCCCCAGGGTCATAGGAGT 71717 750301 503442 503461 GCACTGTCCCAGTTGGATTA 27 718 750302 503490503509 ACCCAGCAAAATGTGGGTCT 119 719 750303 503504 503523TTGGTTGTGGTGAAACCCAG 79 720 750304 503533 503552 TGTTAAAAGAGAAAAGAATC 77721 750305 503550 503569 TTGCAGTGATGTACTGATGT 26 722 750306 503599503618 TTGTTTTTATAAGCAATTAG 59 723 750307 503645 503664TATAAATATGCCCATATGCT 28 724 750308 503656 503675 CTGCCTGCAACTATAAATAT 45725 750309 503825 503844 GTATCTCCTAGCCCAGTGCC 32 726 750310 503828503847 ACTGTATCTCCTAGCCCAGT 64 727 750311 503895 503914GATTGCTTTATTGCAACTAA 63 728 750312 503936 503955 CTTAAATAGTAGGAAAGCCA 14729 750313 503973 503992 ACACTGTACAGGAGGGTGTC 25 155 508739 508758750314 503975 503994 GCACACTGTACAGGAGGGTG 30 730 508741 508760 750315503977 503996 CTGCACACTGTACAGGAGGG 17 79 508743 508762 750316 503979503998 CACTGCACACTGTACAGGAG 41 731 508745 508764 750317 503981 504000TTCACTGCACACTGTACAGG 33 156 508747 508766 750318 503983 504002GATTCACTGCACACTGTACA 34 732 508749 508768 750319 503985 504004TTGATTCACTGCACACTGTA 19 80 508751 508770 750320 503987 504006TGTTGATTCACTGCACACTG 28 733 508753 508772 750321 503991 504010ATGATGTTGATTCACTGCAC 19 734 508757 508776 750322 503993 504012AAATGATGTTGATTCACTGC 43 157 508759 508778 750323 503995 504014GGAAATGATGTTGATTCACT 29 735 508761 508780 750324 503998 504017TGTGGAAATGATGTTGATTC 50 736 750325 504090 504109 CAGTATCTTAACTGGTGAAC 15737

TABLE 12 Reduction of UBE3A-ATS RNA by 8,000 nM 5-10-5 MOE gapmers withmixed PO/PS internucleoside linkages in vitro (40,000 cells/well) SEQ IDSEQ ID UBE3A- Compound NO: 1 NO: 1 ATS SEQ ID Number Start SiteStop Site Sequence (5′ to 3′) (% UTC) NO 617456 463905 463924CAACTGTTACCAAGACTTCA 25 32 617457 465003 465022 ATCTGCGAATAGAGGCCCTC 2733 617460 466539 466558 TAGGTTGCATAAAGCCAGGC 25 36 617461 466981 467000CACACATCTTGTTCCCTCAA 17 37 617557 483977 483996 ATCATGTGCATACCCAGGGT 13172 699781 483977 483996 ATCATGTGCATACCCAGGGT 20 172 750111 481036481055 ACATAGAATTATCATTATGA 49 738 750112 481073 481092ACTGTGGAAAAACTTTGGCA 38 129 750113 481398 481417 ATGGGTTAAAATACTTATAA 79739 750114 481451 481470 TAAAACCTTTGTGCATAATG 32 740 750115 481487481506 CAACAACAAAAGCGGATAAA 50 741 750116 481524 481543ATGACACTAGGTTTTGCAAA 34 742 750117 481529 481548 ACCTCATGACACTAGGTTTT 26743 750118 481571 481590 GTATAGACCCAAACTATAAA 35 744 750119 481573481592 ATGTATAGACCCAAACTATA 55 745 750120 481725 481744TGATCTCTTCAACAAATTGT 82 746 750121 482139 482158 AATGTTGCCGAAAGAAAAGA 73747 750122 482156 482175 ACAAGTGTCATATACTAAAT 51 748 750123 482174482193 AAATTTAACCAAGGAGTTAC 48 749 750124 482246 482265AGCCATCTAAAAGAGAAATT 38 750 750125 482396 482415 GATCAAGAAGTAAAATTATC 74751 750126 482685 482704 CACATGATAATCTCAATTAT 52 752 750127 482916482935 GAAAAATTCACTCTATAGGT 58 753 750128 482988 483007CTATAGAGAGATAGATATTA 52 754 750129 483017 483036 AGAAAAATGTCTTTCCAAAT 43755 750130 483089 483108 CAAATACTTAAATCAACTGT 50 756 750131 483255483274 GGAGGGAGGAAAATTATTTC 14 757 750132 483297 483316AGCAAACAACAGCAACATGC 56 758 750133 483323 483342 AAAATACTTTAGAAAAGTCA 73759 750134 483365 483384 CAGAATTCAATGGACCCACA 33 760 750135 483809483828 TCGGCAAAGGCATTATTATT 32 761 750136 483920 483939GCTTTCACCTATAGGTGGCC 45 762 750137 483928 483947 GATGTTAAGCTTTCACCTAT 25763 750138 483967 483986 TACCCAGGGTAGGATTCATG 17 764 750139 483970483989 GCATACCCAGGGTAGGATTC 13 765 750140 483972 483991GTGCATACCCAGGGTAGGAT 9 766 750141 483975 483994 CATGTGCATACCCAGGGTAG 16767 750142 483979 483998 AGATCATGTGCATACCCAGG 34 768 750143 483982484001 AGAAGATCATGTGCATACCC 36 769 750144 483984 484003TAAGAAGATCATGTGCATAC 44 770 750145 483987 484006 AATTAAGAAGATCATGTGCA119 771 750146 484085 484104 GTTCAGATGAACAATAAGCA 45 772 750147 484311484330 ACTCATGCTGGTTACTAGGG 30 773 750148 484598 484617TCATATGGGTGGTCGCTAAT 24 774 750149 484719 484738 ACACTAACGATGAACTCTAA 55775 750150 484721 484740 TAACACTAACGATGAACTCT 42 776 750151 485136485155 TTAAAACTCAACCCAGTGCA 29 777 750152 485344 485363ATTCCTAGAGAAAACCTAGG 82 778 750153 485478 485497 AGAACAATGTTTCTTATGAA 72779 750154 485865 485884 GAAACTGGGATATTACCAAT 45 780 750155 485871485890 AAAATGGAAACTGGGATATT 37 781 750156 486052 486071AAAAATTAAAGGCCAACAGA 46 782 750157 486157 486176 TTATGGCAGGTAGTGAAAGG 47783 750158 486265 486284 ACAATATGCAAAAATTAAAT 74 784 750159 486400486419 CATTCTCCAACATAGATCCT 41 785 750160 486568 486587CAATAATAGAGACTTTACCA 79 786 750161 486812 486831 ATATATTATGTAAATGTAAC 78787 750162 486824 486843 AGGATGGAAAAGATATATTA 76 788 750163 486842486861 AAACAGGTTGAAAATGAAAG 79 789 750164 487049 487068AAAGGCAATATTGAAGGAAA 72 790 750165 487267 487286 GAATTGAGTTAATAATTCCT 57791 750166 487304 487323 TCTCACAGAGAAAGAGGTGG 21 792 750167 487314487333 TTTTCTATAATCTCACAGAG 43 793 750168 487341 487360GGCTCAGAAAACATCCTTTT 35 794 750169 487437 487456 ATTTAATTTACACTTAATTA 93795 750170 487464 487483 ACTTTCCTCTGCTTATAACT 22 796 750171 487469487488 ACTATACTTTCCTCTGCTTA 28 797 750172 487589 487608AGCAATTAGAAATCACATGA 21 798 750173 487724 487743 AAAGCAGTAAACAATAAGTG 53799 750174 487739 487758 TCATATGTAAATCCAAAAGC 57 800 750175 487772487791 CATTGTAAGGATAAGAGATA 27 801 750176 487784 487803CAGTTTGAATTACATTGTAA 29 802 750177 487804 487823 AATTGAACTTAAATTGGCAT 32803 750178 487831 487850 CTGCATAGGAGTAGAGTTTT 31 804 750179 487877487896 AAGATGGAATTTGCGACATC 50 805 750180 487921 487940ACATAAAAATGTATAAATCC 70 806 750181 488018 488037 ACTGATAAAGGTAAGCACAT 31807

TABLE 13 Reduction of UBE3A-ATS RNA by 8,000 nM 5-10-5 MOE gapmers withmixed PO/PS internucleoside linkages in vitro (40,000 cells/well) SEQ IDSEQ ID UBE3A- Compound NO: 1 NO: 1 ATS SEQ ID Number Start SiteStop Site Sequence (5′ to 3′) (% UTC) NO 617456 463905 463924CAACTGTTACCAAGACTTCA 13 32 617457 465003 465022 ATCTGCGAATAGAGGCCCTC 3833 617460 466539 466558 TAGGTTGCATAAAGCCAGGC 16 36 617461 466981 467000CACACATCTTGTTCCCTCAA 11 37 617557 483977 483996 ATCATGTGCATACCCAGGGT 13172 750398 510078 510097 CAGAGAGGAGAGAAGGACAA 45 808 750399 510104510123 GGCTAGGAAAATTCTCCTGG 39 809 750400 510207 510226AGGGAGAGCTGTCCTAAGGC 27 810 750401 510214 510233 GATATCCAGGGAGAGCTGTC 23811 750402 510269 510288 TTGAAGGGAAAATTATTAAT 43 812 750403 510319510338 AGCTGGACGGACAGTGTTGC 47 813 750404 510407 510426CAAGACATGCTAGGGACTCT 38 814 750405 510408 510427 CCAAGACATGCTAGGGACTC 30815 750406 510416 510435 GCCACAGTCCAAGACATGCT 49 816 750407 510442510461 GCAGATAATCTGAAGATAGA 43 817 750408 510515 510534TTTATTACAAACTGAAAGAG 42 818 750409 510609 510628 GGTCAGATAATGAGGGCCTT 20819 750410 510701 510720 AGGATAGGTGTATGTGAGTG 31 820 750411 510729510748 ATGAAGGTCAAGCCGAATCT 50 821 750412 510798 510817AAAAGAAGTATGTGAAAGTA 45 822 750413 510997 511016 TGTCTCTAATCCTATTCCAA 12823 750414 511100 511119 AGTCTTCATGATTTGGTAAA 37 824 750415 511341511360 AGTAAGAGGCAGAATTATGT 54 825 750416 511441 511460GGGCAGCAACAGCTCTTGAA 16 826 750417 511503 511522 TAATAATTAACAGTGACTGG 24827 750418 511529 511548 AAGTCATTCCCCACCCTGGC ND 828 750419 511555511574 TCCAGCAGGGTGTATTCCAG 47 829 750420 511567 511586TCAGCTGTGGTTTCCAGCAG 34 830 750421 511579 511598 GCTTAGAGAATCTCAGCTGT 49831 750422 511594 511613 GGTTGGAAGCTGCCAGCTTA 22 832 750423 511668511687 AATGGACTGCCGGCCTGGAG 47 833 750424 511893 511912TGAACTACTTGGAGACCTTC 55 834 750425 511895 511914 AGTGAACTACTTGGAGACCT 45835 750426 512282 512301 TCTTTTATTAAACCTAGTTT 60 836 750427 512318512337 TTAGCTAGCTGTGGGTTTGC 25 837 750428 512392 512411ACAAGAGCAGACATCTTTTT 60 838 750429 512564 512583 TGTAGCTTTTCTAAAATTCT 34839 750430 512570 512589 GTATATTGTAGCTTTTCTAA 16 840 750431 513010513029 CTTGATGGCTGTAGCTTGGT 21 841 750432 513074 513093TTTCTGGATTCTCAGTCTTA 28 842 750433 513189 513208 TGCATTGGTATCTGTATATC 23843 750434 513219 513238 GTTTCCTATTTCATCCATTT 25 844 750435 513378513397 CTGGAGTGTAAGTAAATTCA 33 845 750436 513442 513461ATACTCTTTCCACATTTCAG 19 846 750437 513450 513469 TCCTCTTGATACTCTTTCCA 34847 750438 513459 513478 CTTAGGGTCTCCTCTTGATA 32 848 750439 513467513486 CAGAGTGTCTTAGGGTCTCC 27 849 750440 513620 513639ATATTTCTCAAATACTCTTC 48 850 750441 513760 513779 TGTGCTAGAACACTATCTTG 62851 750442 513793 513812 TGACTCTCATCTCCCTCTTC 70 852 750443 513796513815 CCCTGACTCTCATCTCCCTC 50 853 750444 513875 513894CCTTCCTTCTTTATACTGCC 46 854 750445 513944 513963 CATCAAAATCTCTCATTCCT 22855 750446 513947 513966 AATCATCAAAATCTCTCATT 35 856 750447 513951513970 CCCCAATCATCAAAATCTCT 70 857 750448 514039 514058TAGTCAGTAGTCCTAAAAAC 58 858 750449 514073 514092 AGACCTCTGTCTCTGGGATT 54859 750450 514159 514178 AAAGTCCTTACCTGTTCTTG 36 860 750451 514326514345 TTCACCGAAAGTACAGTCTT 54 861 750452 514330 514349TAGTTTCACCGAAAGTACAG ND 862 750453 514439 514458 AAGTCTTTTCTCCCCTCCCC 28863 750454 514504 514523 TCAACAGTGTTAGTGTGTAA 45 864 750455 514513514532 CCTTTTACCTCAACAGTGTT 35 865 750456 514673 514692AGACTAGTCCCTGTGTAGTC 27 866 750457 514676 514695 CCAAGACTAGTCCCTGTGTA 40867 750458 514701 514720 AACTGTCAGAGGAAGAAATG 62 868 750459 514861514880 CGAACAGAACTTGCCGATGT 37 869 750460 514920 514939TTGTATAGGGAGATGATAGA 39 870 750461 515069 515088 TAACCTTTTATTATTATAGA 55871 750462 515111 515130 TAAACTAAAGTAACTGGTTT 55 872 750463 515143515162 CCAGGAAGCTCTGGAGGGAA 23 873 750464 515187 515206ACTCTATTATATATTTTGGT 28 874 750465 515215 515234 GTTATAAAAGTACTTTTTTT 78875 750466 515242 515261 GGATACTTCCTCTACCCCAA 30 876 750467 515247515266 ACAGTGGATACTTCCTCTAC 40 877 750468 515318 515337CTGCCTTTTCATTACTATTT 30 878 750469 515500 515519 GTTTTAAAGTGGTAATTGAA 44879

TABLE 14 Reduction of UBE3A-ATS RNA by 7,000 nM 5-10-5 MOE gapmers withmixed PO/PS internucleoside linkages in vitro (37,000 cells/well) SEQ IDSEQ ID UBE3A- Compound NO: 1 NO: 1 ATS SEQ ID Number Start SiteStop Site Sequence (5′ to 3′) (% UTC) NO 617557 483977 483996ATCATGTGCATACCCAGGGT 44 172 699781 483977 483996 ATCATGTGCATACCCAGGGT 51172 749882 460115 460134 ACTTTATAGTGTGGATGGTA 39 586 750519 34910343491224 GTCATCACCTCTCTTCAGGA 17 181 1065269 432253 432272TCATCATCAACCTAACCGAG 92 880 1065285 433960 433979 CCATCTTGGAACCGGATGAC65 881 1065301 442413 442432 GTAATTGAGGTGGCCATATC 101 882 1065317 443132443151 GAGTCCTAAATGTCTCATAC 81 883 1065333 444649 444668GGTTTTAGTCCACTGGTTCA 44 884 1065349 445540 445559 CTAGTCATGTATCTACAGCC88 885 1065365 446609 446628 GTATATGGCTTACACAGGCT 82 886 1065381 447599447618 GAGATTTCAGACTACCTGTA 64 887 1065397 448125 448144ATTGACCCAGCCCATGGCAC 97 888 1065413 448713 448732 GCGCATTGAGCAAAATTCCA73 889 1065429 449066 449085 GCATAACGATAATCATGTGG 116 890 1065445 449627449646 GGCTATAGGCCATTTATTCA 111 891 1065461 451295 451314TTGAATAACTACCTAGGCTC 86 892 1065477 452310 452329 CCGGTGCAGGAATCCAGGAT98 893 1065493 453797 453816 TAACAATAAGATAAAGGCGC 123 894 1065509 454580454599 GGCAAGCAAAGACTACACCG 55 895 1065525 455169 455188GCTCCTAACATTGTATCCCC 94 896 1065541 455476 455495 CTCTACCAAGCTATCCAAGT78 897 1065557 456768 456787 GTCCTATTGGAGGGCCGCAC 39 898 1065573 457320457339 GTCCTCCAATAAGCTTCTGA 48 899 1065589 458165 458184GGGACAATACTGCAATCCTT 63 900 1065605 458957 458976 AGAGACCTCAAGACCTATAG15 901 1065621 460348 460367 GACTACTTCAACCTGATACC 30 902 1065637 460957460976 GGGCATCATTAACATAAGCT 69 903 1065652 461602 461621ACAAACGGGCTATGTGAGAT 50 904 1065667 463052 463071 CACTGAGTTTTTGTAGTTCG32 905 1065682 463885 463904 GGCAGTTGTGATAGTCAACA 75 906 1065698 464994465013 TAGAGGCCCTCTTGTTTCAA 44 907 1065713 465247 465266GGATTTTATTGGTCATCTCG 30 908 1065729 465664 465683 GTAGTTACTTATACTGGTTC62 909 1065745 466276 466295 GTGGGTTCCTGATGGAGTCC 79 910 1065761 466540466559 CTAGGTTGCATAAAGCCAGG 64 911 1065777 467942 467961GCCTTAAAAGGGTTCCCTGT 64 912 1065793 470287 470306 GGTCATGATGTATGCCATTA39 913 1065809 474125 474144 ATCTAGCTTGAATGGATACC 55 914 1065825 475454475473 GTTCTTTCTCGGTCAAACTA 41 915 1065841 476084 476103TACGAGTTGCTGGTTTCAGC 46 916 1065857 478470 478489 GGTTAGGGCTATGTTATGTT31 917 1065873 480382 480401 GATTCCACTTGTGTATGCAC 33 918 1065889 482148482167 CATATACTAAATGTTGCCGA 30 919 1065905 483368 483387ATACAGAATTCAATGGACCC 55 920 1065936 485657 485676 GCCTAGGACCAGTTGGTTCA48 921 1065952 487409 487428 GGCTGTTGTACATTCCTAGT 65 922 1065968 489096489115 CCCTAAGCTTAGATATACCC 88 923 1065984 489740 489759AGGTGGGAAACCGGTTCCCA 100 924 1066000 493977 493996 GGTCGGTCCCCACCAAAGGA58 925 1066016 496817 496836 GCCATGTACGACCCTCATCA 76 926 1066032 498218498237 TACTATAATACGGGACCTCA 70 927 1066048 499683 499702ATCACATTCAAGGTAGCCCC 48 928 1066064 500605 500624 TGACCAGCAATAGGCTCTGC37 929 1066080 500895 500914 ACTGGCCTTTGGGTCGGATA 81 930 1066096 501395501414 GGAGTCCCCAGATAATGCAT 91 931 1066112 501767 501786GGATGGGCTAAGAGTCACCT 74 932 1066128 502370 502389 GGTATTAAGGCCCTTGGCCA104 933 1066144 502741 502760 GGCCCGATGACACCAGCCAC 87 934 1066160 502949502968 TAACAGTCCTGTAACCAGAC 90 935 1066176 503331 503350CCCCCTGAGATCCATGAGGT 125 936 1066192 503867 503886 CAGTTGTGCAGTTGTTAACT86 937 1066208 504141 504160 AACCAGATGGCTGAGCTATT 89 938 1066224 504518504537 TTAGGCCATACCTCTTCCAT 77 939 1066240 505065 505084TTAGTCTGTTGTGTGACTCC 73 940 1066256 505644 505663 CCACCTAAGAGCTATCCGCT75 941 1066272 505945 505964 GGCCGACCAGCTCTGAAAGT 120 942 1066288 506275506294 ACCAGGGTGGTATTATAAAG 102 943 1066304 506773 506792CAGGTTGTGGAGGTTGTTCC 96 944 1066320 507371 507390 GTTGTCTGGCATGGAGATCC60 945 1066336 507929 507948 GTTGATTGGAGGCACTGCAG 62 946 1066352 508488508507 TGGTGAGTAAATCAATCACG 106 947 1066368 509007 509026CCCTTCAACATGTGTTAACG 62 948 1066384 509917 509936 CGTGGCTGTACCTGCAGTGC49 949 1066400 510393 510412 GACTCTGATTGATTCAGTGC 93 950 1066416 510817510836 AGTACAAACCACTAAGGGTA 114 951 1066432 511629 511648GTGCCATATAATGTTGAAGC 71 952 1066448 511804 511823 CGTGTCTGAACTGGCCTGTG75 953

TABLE 15 Reduction of UBE3A-ATS RNA by 7,000 nM 5-10-5 MOE gapmers withmixed PO/PS internucleoside linkages in vitro (37,000 cells/well) SEQ ID SEQ ID NO: 1 NO: 1 UBE3A- Compound Start Stop ATS SEQ ID NumberSite Site Sequence (5′ to 3′) (% UTC) NO 617557 483977 483996ATCATGTGCATACCCAGGGT 28 172 749882 460115 460134 ACTTTATAGTGTGGATGGTA 25586 750519 349103# 349122# GTCATCACCTCTCTTCAGGA 17 181 1065270 432284432303 CATAACCTCTCCTCGAGACA 96 954 1065286 434182 434201TCTGTCACGCTTCCCTTGGC 61 955 1065302 442470 442489 TCACTGGTGTGGTATCATGC49 956 1065318 443199 443218 GATTGCATCTCTTGCAGCAC 63 957 1065334 444722444741 CTCACCTGTGTATATGAGTC 93 958 1065350 445583 445602ACACTGTTAGAGCCCCCACA 71 959 1065366 446657 446676 GCATAGGAATGGGACCATAG88 960 1065382 447672 447691 GCATCATACATAGTGTATGC 80 961 1065398 448219448238 CTTGTCTCACAATTGTTACC 83 962 1065414 448721 448740TAACTGTAGCGCATTGAGCA 91 963 1065430 449092 449111 GCTAGTCACTCATTGAGAAG110 964 1065446 449633 449652 TAGATAGGCTATAGGCCATT 85 965 1065462 451694451713 GCATATATCTTAACTCACCC 56 966 1065478 452315 452334AAAAGCCGGTGCAGGAATCC 82 967 1065494 453918 453937 TAAGTGCCCATGGCATAGTC65 968 1065510 454719 454738 GAGTGCTGAAGAACATCCCC 75 969 1065526 455213455232 CAAGTCTACTACCAATAAGT 89 970 1065542 455567 455586CGTCAGCATTGCTTGATCTC 89 971 1065558 456773 456792 TATTTGTCCTATTGGAGGGCND 972 1065574 457363 457382 CATGGTGAGGGTCTCAATGA 40 973 1065590 458238458257 GAGACATGTGCCAGTTCAAG 19 974 1065606 458987 459006GGACACTGGCACATCTATAG 64 975 1065622 460362 460381 CTGTATAACTGATTGACTAC51 976 1065638 461068 461087 GCTCATCTATGGATTGCATT 49 977 1065653 461604461623 GTACAAACGGGCTATGTGAG 30 978 1065668 463144 463163AGCTATAGGTACCTGAAGTT 49 979 1065683 464365 464384 CTTAGCTTGCCCAGAGCATA57 980 1065699 464999 465018 GCGAATAGAGGCCCTCTTGT 49 981 1065714 465287465306 GATGTGTATAGGTGTTGGTC 55 982 1065730 465808 465827CCATTTTGGTGCTATAACCC 43 983 1065746 466283 466302 CTTATAGGTGGGTTCCTGAT40 984 1065762 466543 466562 TCTCTAGGTTGCATAAAGCC 69 985 1065778 468007468026 GCACTTGTATAGTTCATCCC 37 986 1065794 470316 470335ATCCTGCATCCGAGGCATGA 76 987 1065810 474167 474186 GTGGTTGCCTTAGTATTACA24 988 1065826 475512 475531 CTGTAAGGTGATATCTATAC 28 989 1065842 476090476109 TGTTCATACGAGTTGCTGGT 52 990 1065858 478533 478552CGCAATGTATCAGGCAACAG 29 991 1065874 480393 480412 GAGGGCACAATGATTCCACT65 992 1065890 482162 482181 GGAGTTACAAGTGTCATATA 37 993 1065906 483424483443 GGCTCTATGCACTTAAGGGA 42 994 1065921 483978 483997GATCATGTGCATACCCAGGG 35 995 1065937 485769 485788 GGTCAGATTCCTAAATACGC26 996 1065953 487600 487619 GTGTCATATGTAGCAATTAG 25 997 1065969 489319489338 CACCTGTATAGGAGAATTGT 100 998 1065985 489776 489795CCACTCCCGTGGCAACATGA 85 999 1066001 493985 494004 GGGTAAGAGGTCGGTCCCCA48 1000 1066017 496821 496840 GGAGGCCATGTACGACCCTC 80 1001 1066033498225 498244 GCTGCAATACTATAATACGG 56 1002 1066049 499792 499811AGCTGAGGTCACCGATCAGA 83 1003 1066065 500610 500629 AACAGTGACCAGCAATAGGC64 1004 1066081 500898 500917 CTAACTGGCCTTTGGGTCGG 107 1005 1066097501399 501418 GGGAGGAGTCCCCAGATAAT 24 1006 1066113 501802 501821GGCCCAAGTGATGACATATC 50 1007 1066129 502410 502429 GTAAGGCATGGTCTTCCTTG79 1008 1066145 502746 502765 AATATGGCCCGATGACACCA 85 1009 1066161502956 502975 GCAAGATTAACAGTCCTGTA 53 1010 1066177 503342 503361TCAGCTCAACACCCCCTGAG 96 1011 1066193 503872 503891 AGGGTCAGTTGTGCAGTTGT39 1012 1066209 504260 504279 GCAAGGAATCATGTGGCTCC 70 1013 1066225504526 504545 CCAAAGATTTAGGCCATACC 55 1014 1066241 505106 505125GTAACCTTCACATAAGCTGC 48 1015 1066257 505654 505673 GGCCTAGCTTCCACCTAAGA104 1016 1066273 505949 505968 CGCTGGCCGACCAGCTCTGA ND 1017 1066289506280 506299 CGTCTACCAGGGTGGTATTA 41 1018 1066305 506779 506798ATACTCCAGGTTGTGGAGGT 84 1019 1066321 507481 507500 CAAATTGGTGAATGTTCCCC44 1020 1066337 508000 508019 TCTCATGACCACCTAATTGA 72 1021 1066353508571 508590 CCGTGCTGCTTTCTTGAGTG 92 1022 1066369 509052 509071ACATGCTTGCATCCAGGCCC 53 1023 1066385 510017 510036 CGGAACCCACAGTAGAGGCA46 1024 1066401 510398 510417 CTAGGGACTCTGATTGATTC 45 1025 1066417510827 510846 ACCAGGCATAAGTACAAACC 62 1026 1066433 511633 511652CTGAGTGCCATATAATGTTG 71 1027 1066449 511822 511841 AAGGCTCTCAGGGTAAGACG42 1028

TABLE 16 Reduction of UBE3A-ATS RNA by 7,000 nM 5-10-5 MOE gapmers with mixedPO/PS internucleoside linkages in vitro (37,000 cells/well) SEQ IDSEQ ID NO: 1 NO: 1 UBE3A- Compound Start Stop ATS SEQ ID Number SiteSite Sequence (5′ to 3′) (% UTC) NO 617557 483977 483996ATCATGTGCATACCCAGGGT 52 172 749882 460115 460134 ACTTTATAGTGTGGATGGTA 22586 750519 349103^(#) 349122^(#) GTCATCACCTCTCTTCAGGA 12 181 1065259430439 430458 CGTGGAACACTGACCCATCA 73 1029 1065275 432915 432934CAGCATGTAACTCATGTTGT 75 1030 1065291 441514 441533 GTTCTAAAGTCGGTTGTGTC57 1031 1065307 442648 442667 AGAGTAGGCCAGGTGTCAAA 60 1032 1065323443577 443596 TACCAGTGAGTAAACTGGCC 89 1033 1065339 444914 444933GTACTCCCCATGCACACTTG 48 1034 1065355 445958 445977 ACGGATTATCATACCCTCCC115 1035 1065371 446805 446824 GTCTTGTAAACCCTTACCAG 83 1036 1065387447818 447837 CCGTTACTTAAGCTGTGGTA 88 1037 1065403 448458 448477TAGTGCCAGTAGGACTTACT 75 1038 1065419 448827 448846 GGACCCTTAAGTCATAAAGA78 1039 1065435 449209 449228 GACCAAATGAGCATCACATC 69 1040 1065451449704 449723 CCTGTATGTATGGCTTATGC 73 1041 1065467 452181 452200ATCCATAGTGATCTGGGAAC 77 1042 1065483 453133 453152 ACTATAATTGGGAATGGTCA77 1043 1065499 454160 454179 GATTTGACCCTTATGGAGAC 104 1044 1065515454855 454874 TATTCCCTATCCAGGGTAGA 96 1045 1065531 455296 455315TTATCCTGTTGCAATAGAAC 81 1046 1065547 456499 456518 ATTAGATTAAAATCTGGCCG93 1047 1065563 456886 456905 GACTCATAAGACAGGATGCC 87 1048 1065579457738 457757 CCAGCCAGGTGTCTTATATC 18 1049 1065595 458523 458542CAGTTGTGTATTGACTAAGT 23 1050 1065611 459549 459568 AAGACTATCCTGGTATGACT56 1051 1065627 460624 460643 TCGATGTTCTTGCTTCCCTG 32 1052 1065642461446 461465 GGCAGCTCCTGACAAATTAG 22 1053 1065658 461694 461713AGACCCCTCCCTTAATGTAA 53 1054 1065673 463249 463268 ACCTGGGTATTGCTGTCCAA41 1055 1065688 464458 464477 CTCACCCTTTACTAAGGTGC 75 1056 1065703465140 465159 CCATTGGTATGAGAGATGCT 72 1057 1065719 465386 465405GTCAGACTTATTGAGGATGG 27 1058 1065735 465932 465951 TTACTCCTCTGGTGTGCTGA29 1059 1065751 466372 466391 GCGCACTTGGGAGCCAGCCA 68 1060 1065767467076 467095 TTACTGACTGGCCTTAGGAG 38 1061 1065783 468351 468370GTAGTATGCATTGACAAGCT 32 1062 1065799 471923 471942 GAGTATATTACCTCCAGGTT27 1063 1065815 474614 474633 GTATTGCTGCTCTAGCTCTA 49 1064 1065831475841 475860 ACGGACCTCATACAGTGAGT 31 1065 1065847 476213 476232GGGCTCTTGGAAGTCTAGTT 59 1066 1065863 479124 479143 ACATACTTGGTTGCAGACGC26 1067 1065879 480825 480844 TAGGTTTGCGGATGCCAGTG 50 1068 1065895482771 482790 TACACAGTGGGATTTGCCCC 59 1069 1065911 483853 483872ATGGCCTGACTAGGCATTGA 76 1070 1065926 484302 484321 GGTTACTAGGGCCAGAGAAT42 1071 1065942 486355 486374 TGTTAATAGACTGCGATTAT 56 1072 1065958487839 487858 GGGCGGAGCTGCATAGGAGT 51 1073 1065974 489522 489541ACAATGGACCACCTAAGACC 96 1074 1065990 489953 489972 CAACCCTTACTCTGCCAGGG80 1075 1066006 494491 494510 TGTGCAATAGCCTAAATGCC 82 1076 1066022497436 497455 CTACTATGAAGTTATGCACC 69 1077 1066038 499100 499119GTTAGCCTTACAGCAAATAC 52 1078 1066054 500359 500378 TACTAATTAGCAAGCCACTG52 1079 1066070 500766 500785 GTGTCAAATCAATTTGTGCC 47 1080 1066086501001 501020 CTAAGCACATCCGATAGTCA 49 1081 1066102 501563 501582GGAGTGCTCTTTGTGGATGC 39 1082 1066118 502117 502136 ACCCATGGCTCATCAGTGGG91 1083 1066134 502447 502466 GGCAGCTCTTTGTAGGCCCA 60 1084 1066150502782 502801 GGAGTGGGTTCCTATAAGGA 53 1085 1066166 503100 503119GGACTAATAGGCCTTTCTAC 61 1086 1066182 503399 503418 CTACAGTACCAGGTCATTTG45 1087 1066198 503946 503965 CACCACCAACCTTAAATAGT 80 1088 1066214504395 504414 CCATGCCACAGATTGGCTTG 64 1089 1066230 504654 504673CGGAGCCTTACGCTTGGCTG 66 1090 1066246 505185 505204 CAGTCTGTCTCTGTGTACCG46 1091 1066262 505741 505760 GGTTGACAGGACATGCTGTC 58 1092 1066278506119 506138 GTTAGCCGAGCATTGGCTTC 84 1093 1066294 506589 506608CCCATGGTGGTGGAATGCTG 102 1094 1066310 506992 507011 TCCCGCACCATGCATTCTGA43 1095 1066326 507684 507703 TCAGCTTCCTTCAGTGGGCG 65 1096 1066342508193 508212 GATCTCACACATGGCACTGC 71 1097 1066358 508691 508710TACCAGTAAAGGGTAGTATA 49 1098 1066374 509110 509129 GTAAATATACACTTGGGCCC73 1099 1066390 510117 510136 TAGCTGAACCTGTGGCTAGG 60 1100 1066406510654 510673 AGTGTCAGGCTGTAAGGGAT 59 1101 1066422 510901 510920GCCATAGGTTTCAGGCTGAT 46 1102 1066438 511707 511726 GAGATGGGCTATGAGCCATC84 1103

TABLE 17 Reduction of UBE3A-ATS RNA by 7,000 nM 5-10-5 MOE gapmers with mixedPO/PS internucleoside linkages in vitro (37,000 cells/well)  SEQ IDSEQ ID NO: 1 NO: 1 Compound Start Stop Sequence UBE3A-ATS SEQ ID NumberSite Site (5′ to 3′) (% UTC) NO 617557 483977 483996ATCATGTGCATACCCAGGGT 32 172 749882 460115 460134 ACTTTATAGTGTGGATGGTA 21586 750519 349103# 349122# GTCATCACCTCTCTTCAGGA 8 181 1065264 430601430620 GGACCAGCGGGCCGAACGCA 101 1104 1065280 433410 433429ATGCAAAGGTGTCGATTTGT 47 1105 1065296 441815 441834 GGTCCATAGATGTCAGTTAC27 1106 1065312 442767 442786 ATTAATGTTCTATGGTGGAT 46 1107 1065328443839 443858 GGAGTAGAATGCCCACTGGG 53 1108 1065344 445212 445231GAGACCTGAATATACCTTAC 55 1109 1065360 446498 446517 GGTATGCCAGGTTTTTGACA49 1110 1065376 447152 447171 CCGTGTAATGACACACCTCT 67 1111 1065392447922 447941 AGGCTATAGTCAAAGGGTGG 46 1112 1065408 448614 448633GTGTCAGTCAATCATTGAGA 75 1113 1065424 449001 449020 GGTCTTATTTACCATTGGCA63 1114 1065440 449470 449489 TAACATGTTGCTTGCACTCC 85 1115 1065456450017 450036 GCTCAAACTAGAATACCCCA 81 1116 1065472 452261 452280GTCTAGGTCTCACGCTGTGT 96 1117 1065488 453235 453254 GACAGTGGAGTCAAGTTGGT106 1118 1065504 454462 454481 GGACTCCACACACCTACTAG 107 1119 1065520455019 455038 GTGGGATGGCTGTCAATGCT 56 1120 1065536 455341 455360GATAATTGAGGAGTTCACCA 81 1121 1065552 220313 220332 GATCCAAACAAGCACCCTCC85 1122 456682 456701 1065568 457020 457039 TATCACAGTTGCTTGACCCT 70 11231065584 457982 458001 GATCTATTATGAGGGCATCA 39 1124 1065600 458571 458590AGCTTTAGTATGTCGAGAAC 20 1125 1065616 460107 460126 GTGTGGATGGTATCCTGGTC26 1126 1065632 460711 460730 GGAATGCTTGATCTGTGGGC 25* 1127 1065647461518 461537 GCTAGACTAGTTGAAATCGG 44 1128 1065663 462056 462075CCCTCCCATCAGTGAAGATT 78 1129 1065677 463767 463786 GCTTTAGAGGTCAAATAGTG50 1130 1065693 464784 464803 CTGGTCTTCCTCGAGGATAG 69 1131 1065708465236 465255 GTCATCTCGGGTATATAAAT 30 1132 1065724 465561 465580GGACAAGTCTTATAGAGAAC 52 1133 1065740 466229 466248 GTTCCAAGTATGGGCTGCTG44 1134 1065756 466426 466445 GTCAACATGTGCTTGCAAGC 35 1135 1065772467425 467444 GCTGTTGATACGCTCCTTCA 37 1136 1065788 469130 469149GAGGCACTGAAGTTCACTAC 37 1137 1065804 472937 472956 AGGGTCCCACTTGTTTAGGT44 1138 1065820 474883 474902 CGTATTAAGGTGTTGTATGA 40 1139 1065836475943 475962 CCTTGTGACAATAGTAGTGA 54 1140 1065852 476443 476462GGCTAGATGGAGCTTGAGCC 94 1141 1065868 479997 480016 CGTGAGCTATCTGTACAAAA24 1142 1065884 481441 481460 GTGCATAATGGTCTACCACA 32 1143 1065900482857 482876 TGAAGATAGGTCAGTTAACG 58 1144 1065916 483925 483944GTTAAGCTTTCACCTATAGG 33 1145 1065931 484601 484620 TGATCATATGGGTGGTCGCT46 1146 1065947 487134 487153 ATTGTCCTCCTGGTAACCAC 28 1147 1065963488572 488591 GGGACTGGTGCCACACCATC 55 1148 1065979 489629 489648CTAACCTGGATCTCAGATAG 75 1149 1065995 493844 493863 CTGGGCCCCAATTCAGTAAT98 1150 1066011 495399 495418 TTGGTAAAGGAGGGAATCGG ND 1151 1066027498030 498049 TCCAGGATATATGTTAGTCC 46 1152 1066043 499291 499310TAGCTACAATAAGTTGTAGC 94 1153 1066059 500494 500513 GAGGGCCATGTTAAAGGCCT119 1154 1066075 500861 500880 TTTTGGTAGGTAACTACGGG 41 1155 1066091501335 501354 TGTAGCTCAGCTCAATGTGT 58 1156 1066107 501628 501647GAACTGCACTGGGTTGTCTC 58 1157 1066123 502162 502181 GTATTGCACTCACATACTGT61 1158 1066139 502579 502598 AGCCAATGCGCAAGAAAAGT 69 1159 1066155502824 502843 CATAGGCATAAAGCCTCCTA 79 1160 1066171 503139 503158TCCCAGAAATGGTCCTCGCA 86 1161 1066187 503480 503499 ATGTGGGTCTGCACCAAGTT86 1162 1066203 504077 504096 GGTGAACTCCTGTGACTGAT 52 1163 1066219504441 504460 GTGGAGTAGGTATATTAGTC 32 1164 1066235 504825 504844GTATTCCTGAAGTAGTCCTG 36 1165 1066251 505330 505349 AGAAATTGGGCCGCCTCTGT58 1166 1066267 505817 505836 GTGCTTAGTGAACTGTGGGC 55 1167 1066283506185 506204 CCGGCATGCATCAGCTCTGA 34 1168 1066299 506693 506712TAAGAAGCTTGCCTTTCGAT 96 1169 1066315 507250 507269 GCAGTGCTACTGTGCCCTTA58 1170 1066331 507818 507837 TCCAGCCCTCAGTATATAGA 62 1171 1066347508285 508304 CCGAAGTGGAAGTAGTCATG 63 1172 1066363 508790 508809TGAATGCCACCGTGATTGCA 50 1173 1066379 509416 509435 CGTAGTGTCATCACCATAAA45 1174 1066395 510227 510246 GTTGTGTCTGAGGGATATCC 45 1175 1066411510718 510737 GCCGAATCTTGACATACAGG 55 1176 1066427 511241 511260GATTACCAAAAAGGGACCAG 48 1177 1066443 511766 511785 CTCCTACCAATAATGGAGTC76 1178

TABLE 18Reduction of UBE3A-ATS RNA by 7,000 nM 5-10-5 MOE gapmers with mixedPO/PS internucleoside linkages in vitro (37,000 cells/well) SEQ IDSEQ ID NO: 1 NO: 1 Compound Start Stop  Sequence UBE3A-ATS SEQ ID Number Site Site (5′ to 3′) (% UTC) NO 617557 483977 483996ATCATGTGCATACCCAGGGT 38 172 749882 460115 460134 ACTTTATAGTGTGGATGGTA 32586 750519 349103# 349122# GTCATCACCTCTCTTCAGGA 17 181 1065265 430612430631 CGGACACACTGGGACCAGCG 90 1179 1065281 433463 433482GCTCCTATCAAGCTTTTCCC 46 1180 1065297 441909 441928 TGAGGTGCATTTTCTAGCCC78 1181 1065313 442830 442849 ATCTATGGAGTCATCTCCCC 76 1182 1065329443979 443998 GGTAGAGGTTAATCTATGTC 59 1183 1065345 445310 445329CGTCGGTAAAGGAAGCTACT 58 1184 1065361 446510 446529 TCATAAGGACAAGGTATGCC73 1185 1065377 447310 447329 GGACCTCATTAGATCAGTCA 90 1186 1065393448007 448026 GGCAGTTGAGTGGTGTCAGT 99 1187 1065409 448628 448647GGATTGCATCACATGTGTCA 55 1188 1065425 449017 449036 TATAAGTGTCCATAAAGGTC90 1189 1065441 449475 449494 GGCATTAACATGTTGCTTGC 66 1190 1065457450095 450114 GAATTGATAAGTGGTCTTCT 86 1191 1065473 452266 452285GGGAAGTCTAGGTCTCACGC ND 1192 1065489 453243 453262 CTACATATGACAGTGGAGTC89 1193 1065505 454467 454486 TGAGTGGACTCCACACACCT 84 1194 1065521455028 455047 GATATTGCAGTGGGATGGCT 60 1195 1065537 455360 455379CGTGATAGTGCTATTGTGAG 60 1196 1065553 220317 220336 CATCGATCCAAACAAGCACC74 1197 456686 456705 1065569 457233 457252 GCTTAGCTACTCACCCCTGT 70 11981065585 457989 458008 CCCACTTGATCTATTATGAG 64 1199 1065601 458790 458809CCCTAATATAGGGCAGATGA 59 1200 1065617 460108 460127 AGTGTGGATGGTATCCTGGT44 1201 1065633 460736 460755 GGATTTGAGCCTGCTATGTC 15* 1202 1065648461524 461543 CATTCAGCTAGACTAGTTGA 70 1203 1065664 462061 462080TATGGCCCTCCCATCAGTGA 49 1204 1065678 463775 463794 TAGATTGGGCTTTAGAGGTC33 1205 1065694 464788 464807 ACATCTGGTCTTCCTCGAGG 61 1206 1065709465237 465256 GGTCATCTCGGGTATATAAA 17 1207 1065725 465591 465610ACCGAAGGAGTTCCTTTAGC 40 1208 1065741 466233 466252 GGCAGTTCCAAGTATGGGCT76 1209 1065757 466438 466457 GTGGAGCCAGCTGTCAACAT 66 1210 1065773467718 467737 TGGAATGTATCCTGTACGGG 52 1211 1065789 469377 469396GTGTTATACTATTGTGGTGC 42 1212 1065805 472944 472963 GTTTGTTAGGGTCCCACTTG49 1213 1065821 474901 474920 TCTGCTATTGTTGGATATCG 33 1214 1065837475988 476007 ATGGTTAGTTTAAGAAATCG 65 1215 1065853 478295 478314GAGTCCTGGTTGATGTGGTG 65 1216 1065869 480141 480160 ACGGGCATGCTTTATAATTA73 1217 1065885 481448 481467 AACCTTTGTGCATAATGGTC 82 1218 1065901482908 482927 CACTCTATAGGTTCAAGCAG 37 1219 1065917 483964 483983CCAGGGTAGGATTCATGGTC 63 1220 1065932 484673 484692 TGTTGTATGCAGGTTCATCA31 1221 1065948 487139 487158 GATACATTGTCCTCCTGGTA 35 1222 1065964488637 488656 ACACTTGAAGCTGTTGAGTC 66 1223 1065980 489652 489671CGTGAAGGAATGATCTCTCT 61 1224 1065996 493855 493874 CCTAATCTATGCTGGGCCCC75 1225 1066012 495430 495449 GAGAGTAAGTTACTAGAGGC 59 1226 1066028498180 498199 TGGGCAGATTGATCACCTAG 93 1227 1066044 499495 499514TACTAGGCCTGCTCTACTGG 71 1228 1066060 500523 500542 TTAGAGGTCAAGCCCTGTGT63 1229 1066076 500885 500904 GGGTCGGATATAGCTTTTAC 31 1230 1066092501340 501359 GCTTATGTAGCTCAGCTCAA 33 1231 1066108 501701 501720TTGGGACATCCCAAAGTTAC 80 1232 1066124 502205 502224 GGAACATCATGTTAGCCATC44 1233 1066140 502588 502607 GATGCATCAAGCCAATGCGC 41 1234 1066156502882 502901 GAACCTCTACAGAGAGACTA 65 1235 1066172 503154 503173GTTCTGTATACACCATCCCA 56 1236 1066188 503485 503504 GCAAAATGTGGGTCTGCACC96 1237 1066204 504082 504101 TAACTGGTGAACTCCTGTGA 98 1238 1066220504449 504468 CCCCAGAAGTGGAGTAGGTA ND 1239 1066236 504866 504885CCTCAAGATCAACAGACCCC 81 1240 1066252 505543 505562 CAGTCAGGTACAGGTGTTGG52 1241 1066268 505823 505842 GTGAGGGTGCTTAGTGAACT 54 1242 1066284506196 506215 GTCAGAACAATCCGGCATGC 50 1243 1066300 506730 506749GAGTGCTCCACACTTCTGTC 65 1244 1066316 507254 507273 GCTTGCAGTGCTACTGTGCC95 1245 1066332 507900 507919 TCGATACCTGCTTTTGTGAC 69 1246 1066348508290 508309 CATAACCGAAGTGGAAGTAG 71 1247 1066364 508901 508920TACTTCATGACTGCCTAGTT 78 1248 1066380 509434 509453 AAGAAGGGCATATATCTACG72 1249 1066396 510312 510331 CGGACAGTGTTGCTGTTAGG 39 1250 1066412510724 510743 GGTCAAGCCGAATCTTGACA 68 1251 1066428 511490 511509TGACTGGGTAAGGCAGGATC 50 1252 1066444 511770 511789 AGTACTCCTACCAATAATGG77 1253

TABLE 19 Reduction of UBE3A-ATS RNA by 7,000 nM 5-10-5 MOE gapmers withmixed PO/PS internucleoside linkages in vitro (37,000 cells/well) SEQ IDSEQ ID NO: 1 NO: 1 UBE3A- Compound Start Stop Sequence ATS SEQ ID NumberSite Site (5′ to 3′) (% UTC) NO 617557 483977 483996ATCATGTGCATACCCAGGGT 17 172 749882 460115 460134 ACTTTATAGTGTGGATGGTA 26586 750519 349103# 349122# GTCATCACCTCTCTTCAGGA 12 181 1065271 432577432596 GGCAGTGCATTCAACTGTAG 75 1254 1065287 440156 440175CGTGGGTTAGTCCAAGTAAC 49 1255 1065303 442546 442565 GCCCATTATGTAGTCTATAG37 1256 1065319 443234 443253 GACGAAGGCAGTCACTCTGC 88 1257 1065335444735 444754 GGGAGAACTACATCTCACCT 63 1258 1065351 445658 445677CCCCATTACCACGGTCTCTT 63 1259 1065367 446675 446694 AGCAGTCTTTATCTACAGGC85 1260 1065383 447745 447764 TTGATGACCTGGTAAGGGAT 76 1261 1065399448290 448309 CGCAATAAGATTCCATTGCC 83 1262 1065415 448725 448744ACGATAACTGTAGCGCATTG 70 1263 1065431 449100 449119 GCAAGTATGCTAGTCACTCA82 1264 1065447 449637 449656 CCATTAGATAGGCTATAGGC 77 1265 1065463452066 452085 TCGCCGCTGATTTTAAAGAT 97 1266 1065479 452320 452339GGTTCAAAAGCCGGTGCAGG 83 1267 1065495 453926 453945 GTTGTTCTTAAGTGCCCATG61 1268 1065511 454740 454759 GCACTTTGCATGAGAATAGG 60 1269 1065527455219 455238 TCCAGACAAGTCTACTACCA 69 1270 1065543 455585 455604TCTACTCCTTGCTACAAACG 84 1271 1065575 457378 457397 CAATAGTGTAGGTGACATGG36 1273 1065591 458400 458419 GCATGTGGGCCTCTATTAAG 8 1274 1065607 459079459098 GTATGGTATCTTGCAGTAGC 15 1275 1065623 460427 460446ATAGCCCTTTCAGCACACTT 16 1276 1065639 461343 461362 GCTGGCAATATGTCAACCTT36 1277 1065654 461605 461624 TGTACAAACGGGCTATGTGA 30 1278 1065669463151 463170 CATATGAAGCTATAGGTACC 22 1279 1065684 464383 464402GGTAGTTCACAACTCTTCCT 59 1280 1065700 465002 465021 TCTGCGAATAGAGGCCCTCT49 1281 1065715 465301 465320 GACACGCTGACTATGATGTG 77 1282 1065731465875 465894 CTTGCCCATGGATGGTTGTC 71 1283 1065747 466287 466306GTGGCTTATAGGTGGGTTCC ND 1284 1065763 466945 466964 GGTTGTATGCCTTCTGCATT44 1285 1065779 468014 468033 CCTTGCAGCACTTGTATAGT 44 1286 1065795470319 470338 ACTATCCTGCATCCGAGGCA 23 1287 1065811 474227 474246ATATAATTGAGGGCCACCAT 74 1288 1065827 475597 475616 CCTTTAGAGGGATTTGTGTA34 1289 1065843 476097 476116 CTTAACATGTTCATACGAGT 47 1290 1065859478733 478752 GCTGATCACATTACCCATCC 24 1291 1065875 480518 480537GATGTATCACGCAAACAATT 81 1292 1065891 482167 482186 ACCAAGGAGTTACAAGTGTC62 1293 1065907 483587 483606 GGCCAGGATGGTCAACCTTA 50 1294 1065922484119 484138 TTTTTGCCACAGCCGCTTGG 193 1295 1065938 485811 485830CGAATAATTGCATGCCAACT 68 1296 1065954 487606 487625 GGTTTAGTGTCATATGTAGC23 1297 1065970 489324 489343 GCACACACCTGTATAGGAGA 43 1298 1065986489780 489799 CCTGCCACTCCCGTGGCAAC 95 1299 1066002 493989 494008TTATGGGTAAGAGGTCGGTC ND 1300 1066018 496826 496845 GGTATGGAGGCCATGTACGA84 1301 1066034 498510 498529 GCGCCCGGCAAGAGATTCAC ND 1302 1066050499912 499931 TTGCATAATAGGAGGTCCTT 51 1303 1066066 500662 500681GGGCTTACAACCTCTCAATT 74 1304 1066082 500899 500918 CCTAACTGGCCTTTGGGTCG80 1305 1066098 501446 501465 GTCTGCACAGGTGGCATAGA 53 1306 1066114501817 501836 GTCCAGAGGTCATATGGCCC 54 1307 1066130 502416 502435GCATAAGTAAGGCATGGTCT 41 1308 1066146 502755 502774 CTTCGGGCAAATATGGCCCG76 1309 1066162 503036 503055 GGTGCTACAGTCATACTATC 62 1310 1066178503346 503365 GTGATCAGCTCAACACCCCC 65 1311 1066194 503878 503897TAATAAAGGGTCAGTTGTGC 39 1312 1066210 504270 504289 CACTGCCTAAGCAAGGAATC86 1313 1066226 504530 504549 TGCCCCAAAGATTTAGGCCA 69 1314 1066242505115 505134 TCAGACATGGTAACCTTCAC 40 1315 1066258 505660 505679ATTGCAGGCCTAGCTTCCAC 51 1316 1066274 506031 506050 GGTATTGAGTAGGACTTCTC34 1317 1066290 506402 506421 CTTAAGGAGGCAACTCCTGA 51 1318 1066306506781 506800 ATATACTCCAGGTTGTGGAG 66 1319 1066322 507526 507545GAGCCAGGTTCCTGTTCACG 33 1320 1066338 508110 508129 CCGTCTTTAGGAACTTAAAT43 1321 1066354 508581 508600 AGCACAACCCCCGTGCTGCT 88 1322 1066370509058 509077 TACCCAACATGCTTGCATCC 58 1323 1066386 510022 510041ATACACGGAACCCACAGTAG 70 1324 1066402 510402 510421 CATGCTAGGGACTCTGATTG69 1325 1066418 510832 510851 GTTAGACCAGGCATAAGTAC 44 1326 1066434511638 511657 TGCAGCTGAGTGCCATATAA 56 1327 1066450 511890 511909ACTACTTGGAGACCTTCACC 57 1328

TABLE 20Reduction of UBE3A-ATS RNA by 7,000 nm 5-10-5 MOE gapmers with mixedPO/PS internucleoside linkages in vitro (37,000 cells/well) SEQ IDSEQ ID NO: 1 NO: 1 Compound Start Stop Sequence UBE3A-ATS SEQ ID  NumberSite Site (5′ to 3′) (% UTC) NO 617557 483977 483996ATCATGTGCATACCCAGGGT 35 172 749882 460115 460134 ACTTTATAGTGTGGATGGTA 26586 749902 461442 461461 GCTCCTGACAAATTAGCACT 61 1329 750519 349103^(#)349122^(#) GTCATCACCTCTCTTCAGGA 20 181 1065258 430419 430438CGGATGCAGGGCACCCTTGG 96 1330 1065274 432770 432789 CATGATATAAGGAGAAACGC63 1331 1065290 440729 440748 ATCTTGCGGCTGTCTCCCAG 64 1332 1065306442613 442632 TCCCATAGTTATATGGCTCA 60 1333 1065322 443476 443495CTGCATGTTAGATTGGGCAT 48 1334 1065338 444879 444898 GCTGTGATGCTAGGATGCAT84 1335 1065354 445874 445893 AGACATAGACCCCAGACGGT 83 1336 1065370446795 446814 CCCTTACCAGAATAGCATCC 81 1337 1065386 447803 447822TGGTAGACCACTGATTCCCC 63 1338 1065402 448415 448434 AGCAAGGGTCTTAGTGCCAA60 1339 1065418 448742 448761 GTCTGTATATTTAGACCACG 83 1340 1065434449159 449178 GTCAACATTATATCGATGCA 64 1341 1065450 449697 449716GTATGGCTTATGCATGCTAT 86 1342 1065466 452137 452156 TGGGCAGGTCAACTTGGTAT66 1343 1065482 453037 453056 AAAAGGTGATTAGGCGGCCG 88 1344 1065498454156 454175 TGACCCTTATGGAGACTTAT 74 1345 1065514 454846 454865TCCAGGGTAGAAGACTAGCA 63 1346 1065530 455286 455305 GCAATAGAACAGATGGCCAT97 1347 1065546 455764 455783 GTACCAAAGTGGCTGCTCAC 89 1348 1065562456866 456885 ACTGCCCTCTTCGAAGAGAT 71 1349 1065578 457715 457734GATTATCTCAGACATGCCAT 20 1350 1065594 458488 458507 CCTTAAGCAAGGAGTTCACT39 1351 1065610 459543 459562 ATCCTGGTATGACTGTCAGT 45 1352 1065626460538 460557 GGCTCTCATCTAAATAAGCC 75 1353 1065657 461674 461693AACCCTAAGGTGAAGTCTGT 65 1354 1065672 463245 463264 GGGTATTGCTGTCCAAATGG20 1355 1065687 464409 464428 GTGTTATGGTTCCCATTCAG 32 1356 1065702465012 465031 CCTGCTCAAATCTGCGAATA 58 1357 1065718 465382 465401GACTTATTGAGGATGGTGTG 53 1358 1065734 465894 465913 TGAACTCCCACAAGGTACTC52 1359 1065750 466341 466360 AGGCATTTGGAGCATTCGGG 16 1360 1065766467050 467069 ACTTTCATCAGTTAGTCAGG 30 1361 1065782 468343 468362CATTGACAAGCTATTGCAGC 44 1362 1065798 470982 471001 TTTTATGGCTTATAGCAGCG50 1363 1065814 474563 474582 CACGCCCAAATGGAACTCTA 38 1364 1065830475827 475846 GTGAGTTATAGAGTGTTCCC 39 1365 1065846 476165 476184TGGGCACTTAGGAGTTCCTA 73 1366 1065862 479069 479088 GCTGTATCTGTGGTTTAGCA67 1367 1065878 480818 480837 GCGGATGCCAGTGGCCGAGA 40 1368 1065894482519 482538 GCTATACCCCTAGGATCAGA 31 1369 1065910 483843 483862TAGGCATTGAATGAGGGCCC 71 1370 1065925 484245 484264 GTATGGACTTGTCTTATGGC57 1371 1065941 486351 486370 AATAGACTGCGATTATACAA 48 1372 1065957487807 487826 CGCAATTGAACTTAAATTGG 56 1373 1065973 489517 489536GGACCACCTAAGACCTCAAG 50 1374 1065989 489908 489927 GGCTAAAGTAATCTTATGGG48 1375 1066005 494490 494509 GTGCAATAGCCTAAATGCCA 69 1376 1066021497267 497286 GGATTAGGCAGCTTCACTAC 44 1377 1066037 499088 499107GCAAATACAATGGTAATCGC 30 1378 1066053 500286 500305 CTGGTGTACATTGGATATGA57 1379 1066069 500694 500713 GCAAGAGGTACTGTAAGCCC 70 1380 1066085500993 501012 ATCCGATAGTCAAACTATGA 52 1381 1066101 501532 501551AAACACCCTTCCAATGAGGG 75 1382 1066117 501986 502005 GTTCACTAGCATCTGCTACA46 1383 1066133 502443 502462 GCTCTTTGTAGGCCCAAGGG 38 1384 1066149502776 502795 GGTTCCTATAAGGAATAGGC 73 1385 1066165 503096 503115TAATAGGCCTTTCTACAGCT 77 1386 1066181 503374 503393 CCCCAGGGTCATAGGAGTGT80 1387 1066197 503944 503963 CCACCAACCTTAAATAGTAG 70 1388 1066213504390 504409 CCACAGATTGGCTTGGAATG 74 1389 1066229 504650 504669GCCTTACGCTTGGCTGACAT 73 1390 1066245 505179 505198 GTCTCTGTGTACCGAGCTCA63 1391 1066261 505724 505743 GTCTGGTGGCCAAGTGCTTC 70 1392 1066277506116 506135 AGCCGAGCATTGGCTTCATA 73 1393 1066293 506584 506603GGTGGTGGAATGCTGTCCAC 89 1394 1066309 506869 506888 CTCCACCAAATACTTAGCCC67 1395 1066325 507649 507668 TAAATGTCAGGAGGTCCCCC 55 1396 1066341508126 508145 CCTTGGGTTATTCTTACCGT 55 1397 1066357 508680 508699GGTAGTATAAGAATGGTTCC 54 1398 1066373 509104 509123 ATACACTTGGGCCCAAATGG78 1399 1066389 510039 510058 GCTAGCATTTGAGAGTTATA 43 1400 1066405510647 510666 GGCTGTAAGGGATTAAGATG 79 1401 1066421 510853 510872CTGTTACAGGGAGACAATCT 56 1402 1066437 511678 511697 ACCCTGCACAAATGGACTGC103 1403

TABLE 21Reduction of UBE3A-ATS RNA by 7,000 nM 5-10-5 MOE gapmers with mixedPO/PS internucleoside linkages in vitro (37,000 cells/well) SEQ IDSEQ ID NO: 1 NO: 1 UBE3A- Compound Start Stop ATS SEQ ID Number SiteSite Sequence (5′ to 3′) (% UTC) NO 617557 483977 483996ATCATGTGCATACCCAGGGT 42 172 749882 460115 460134 ACTTTATAGTGTGGATGGTA 33586 750519 349103^(#) 349122^(#) GTCATCACCTCTCTTCAGGA 21 181 1065268432234 432253 GGGCACCCCCGTCACCGTGC 82 1404 1065284 433765 433784AGATTTTCCGACCTATGTCA 62 1405 1065300 442386 442405 ATCGCCATGGTCACTTGCCA63 1406 1065316 443032 443051 GGCAAAGCAAGTTCGCCACA 91 1407 1065332444610 444629 ATCATACAGGTCACTTGGCT 63 1408 1065348 445505 445524ATTGGACTAGGTAACAGTGA 103 1409 1065364 446595 446614 CAGGCTATTTCTTAGTGGAC59 1410 1065380 447562 447581 CAAGGGTAACCTATAGCTGA 116 1411 1065396448099 448118 GCATATGCACACTTGGATCA 89 1412 1065412 448676 448695ACCCTGAAAACAATCAATCG 102 1413 1065428 449061 449080 ACGATAATCATGTGGCCTGA85 1414 1065444 449593 449612 GACTAACTATACAGGTCTCT 107 1415 1065460451288 451307 ACTACCTAGGCTCACTTGCT 83 1416 1065476 452297 452316CCAGGATGCTAATGCTCCTA 69 1417 1065492 453781 453800 GCGCTTTCACCAAGAAATTT78 1418 1065508 454575 454594 GCAAAGACTACACCGTGACA 109 1419 1065524455153 455172 CCCCTGCATTCAGCTTATAG 85 1420 1065540 455467 455486GCTATCCAAGTGACACAGTA 77 1421 1065556 456757 456776 GGGCCGCACATCTGGACCTC104 1422 1065572 457293 457312 TACAAAACGACCTAAAGACC 83 1423 1065588458156 458175 CTGCAATCCTTAAGACTTGA 37 1424 1065604 458952 458971CCTCAAGACCTATAGGACCT 37 1425 1065620 460284 460303 ATACATTGCCAGCACTAAGG60 1426 1065636 460849 460868 GATTTACCACACATATAGGC 36 1427 1065651461597 461616 CGGGCTATGTGAGATAATTC 15 1428 1065666 462686 462705GGCTGACATGCCCCTTTTAA 142 1429 1065681 463881 463900 GTTGTGATAGTCAACAATTG89 1430 1065697 464991 465010 AGGCCCTCTTGTTTCAATTG 63 1431 1065712465246 465265 GATTTTATTGGTCATCTCGG 30 1432 1065728 465606 465625GCCTTTCTATTTCAGACCGA 35 1433 1065744 466263 466282 GGAGTCCATGAAGTAACTGG62 1434 1065760 466538 466557 AGGTTGCATAAAGCCAGGCC 55 1435 1065776467801 467820 CCCTCTTAGTGATTGGTGGT 63 1436 1065792 470049 470068GGTTGGCAGTCTTCACCAGT 62 1437 1065808 474120 474139 GCTTGAATGGATACCAATTA40 1438 1065824 475447 475466 CTCGGTCAAACTAATAATAC 53 1439 1065840476015 476034 CCAGTTTTCGCCCGTTACCT 32 1440 1065856 478440 478459GTAGCTATAGGTGTCACATA 21 1441 1065872 480351 480370 GCCTCAAATATGTGATGCAC45 1442 1065888 482108 482127 GAATGGCAATACATCCGTGT 80 1443 1065904483359 483378 TCAATGGACCCACATGATCA 63 1444 1065920 483971 483990TGCATACCCAGGGTAGGATT 29 1445 1065935 485084 485103 TCTAATACTACAACGATGGA84 1446 1065951 487204 487223 AACCTTCCTAAAATCCCCGA 52 1447 1065967489085 489104 GATATACCCAGTTAATCAGT 63 1448 1065983 489731 489750ACCGGTTCCCAATTTTCTCC 78 140 1065999 493889 493908 TTGGCAGATGTAACCTATTC52 1449 1066015 496809 496828 CGACCCTCATCACTTTTTGA 73 1450 1066031498209 498228 ACGGGACCTCAATACTCTAC 56 1451 1066047 499678 499697ATTCAAGGTAGCCCCAATAC 89 1452 1066063 500578 500597 CTACTGGCATCAGTCAAAAC84 150 1066079 500894 500913 CTGGCCTTTGGGTCGGATAT 86 1453 1066095 501390501409 CCCCAGATAATGCATAGATC 98 1454 1066111 501763 501782GGGCTAAGAGTCACCTGTAT 59 1455 1066127 502360 502379 CCCTTGGCCACCTGACTTCG77 1456 1066143 502629 502648 TTGGCTCAGTGTTCACTTCG 52 1457 1066159502939 502958 GTAACCAGACCCAAGGCACT 62 1458 1066175 503325 503344GAGATCCATGAGGTATATAC 55 1459 1066191 503815 503834 GCCCAGTGCCCTATAGGTCA67 1460 1066207 504127 504146 GCTATTTCATTAAGTCACCC 39 1461 1066223504502 504521 CCATGGAATGGCTGTCATGC 72 1462 1066239 504974 504993TTATCTTCTTAGGGTCGACT 38 1463 1066255 505635 505654 AGCTATCCGCTTCCCAAGGG47 1464 1066271 505906 505925 CCCTGAGATGCTAGTTGGGC 85 1465 1066287506256 506275 GTATGTCCTTGGAGGTGAGC 88 1466 1066303 506744 506763GCCTTTCATTTTGGGAGTGC 48 1467 1066319 507364 507383 GGCATGGAGATCCAACCTGT96 1468 1066335 507919 507938 GGCACTGCAGGATAGCCATT 95 1469 1066351508470 508489 CGTTATCCTAAGAAGTGACT 58 1470 1066367 509003 509022TCAACATGTGTTAACGGAAC 63 1471 1066383 509910 509929 GTACCTGCAGTGCATAGAGC52 1472 1066399 510387 510406 GATTGATTCAGTGCTCATGC 49 1473 1066415510747 510766 ACTGCAGATAGGTAGGTGAT 43 1474 1066431 511593 511612GTTGGAAGCTGCCAGCTTAG 96 1475 1066447 511795 511814 ACTGGCCTGTGGCAGTTAAC80 1476

TABLE 22 Reduction of UBE3A-ATS RNA by 7,000 nM 5-10-5 MOE gapmers withwith mixed PO/PS internucleoside linkages in vitro (37,000 cells/well)SEQ ID SEQ ID NO: 1 NO: 1 UBE3A- Compound Start Stop  ATS SEQ ID  NumberSite Site Sequence (5′ to 3′) (% UTC) NO 617557 483977 483996ATCATGTGCATACCCAGGGT 28 172 749882 460115 460134 ACTTTATAGTGTGGATGGTA 24586 750519 349103^(#) 349122^(#) GTCATCACCTCTCTTCAGGA 17 181 1065261430489 430508 GCGCTGGCTCACGCATGCTC 65 1477 1065277 432989 433008CCTAGAGTTTCCTTCTCCCG 79 1478 1065293 441703 441722 GGGATGCCAATGCCTCCAAC97 1479 1065309 442707 442726 TAAATTGCCCTGGGTGCAGC 71 1480 1065325443778 443797 GCCCTTTCAGGACCAACTGG 107 1481 1065341 445116 445135GTTGGGCAAACAAGTTACCC 92 1482 1065357 446183 446202 GGGACCCCAATTTACTACGC112 1483 1065373 446912 446931 CTTCTTGCAAGGATTGGCAC 69 1484 1065389447847 447866 ACTGAGATGTGTGATACTCC 70 1485 1065405 448471 448490CCACTCAATAGAATAGTGCC 123 1486 1065421 448838 448857 CCCAATTGCAAGGACCCTTA80 1487 1065437 449334 449353 GAACATGTAGCCATAATGCC 65 1488 1065453449867 449886 GAGTGGCATCCTTAAATCCT 66 1489 1065469 452204 452223TAATTGGTCAAGTAAACAGC 73 1490 1065485 453199 453218 TCCACCGTTACTGATTATCT51 1491 1065501 454230 454249 CAAGTCCCACCATGTTAATC 87 1492 1065517454913 454932 GTTAGGATCTATTTGACAGC 67 1493 1065533 455318 455337AGGCCTGCTCCGAATGTGTT 107 1494 1065549 456627 456646 GTGCATGGTTATCTAATGCA87 1495 1065565 456917 456936 TTGGGTCACTAGGCACACTA 110 1496 1065581457899 457918 TGACTAGTTCGATAAGTTTT 48 1497 1065597 458561 458580TGTCGAGAACTCAAAAGGTG 31 1498 1065613 459646 459665 CTGTATAGTTTACCCAGGCA24 1499 1065629 460641 460660 CAGAGTGCATTGGCAACTCG 50 1500 1065644461457 461476 GATCTTGGTAAGGCAGCTCC 29 1501 1065660 461891 461910CAATTTGGTCCCATTGTAGT 84 1502 1065675 463503 463522 TCTATCAAGGCTGTTATTGG40 1503 1065690 464528 464547 GCTCAGTTAGGTTAGTGCAC 23 1504 1065705465162 465181 TCTAGGGCTCCAGTTTATGT 78 1505 1065721 465442 465461CTTTGGTTATGTACATTGCC 73 1506 1065737 466098 466117 GCCTGTATGTCTTGAGAAAC78 1507 1065753 466388 466407 CTCGCTTTTCACAGGAGCGC 36 1508 1065769467300 467319 TCACAGAGTAGTCTATTGGT 43 1509 1065785 468922 468941GTAAGTATAGATGCCTCTCC 33 1510 1065801 472475 472494 GCAAACTTTAGGAGTGTGTT60 1511 1065817 474799 474818 GGTATTGACACCTCCAACTG 18 1512 1065833475854 475873 ATATAAGGGTAATACGGACC 39 1513 1065849 476362 476381GCCCCCTGCCGTGTGAAAGA 72 1514 1065865 479579 479598 CTTCAAGACTAAGGTAGGGA38 1515 1065881 480923 480942 GTATGCGAAGCGAACGAAGC 75 1516 1065897482786 482805 CCTAAAAAACCCGTGTACAC 62 1517 1065913 483907 483926GGTGGCCTTCAGTCAGTACA 57 1518 1065928 484317 484336 GCCCAGACTCATGCTGGTTA96 1519 1065944 486448 486467 ATACTTCACCTAATAGCACC 86 1520 1065960488086 488105 GGAGTTCTTTAGGTTGGAAC 62 1521 1065976 489542 489561GCAACTATGGGTGGAGACAT 53 1522 1065992 493191 493210 TCGGGCCAGGTCCAGGCGCA52 1523 1066008 495127 495146 GCTGTCATATGGGAACTACG 57 1524 1066024497743 497762 CAAACCTACGCCAATAAAGA 86 1525 1066040 499138 499157TTTATCGCTTAAAGTAGCCT 83 1526 1066056 500407 500426 CGTATATCGAATACCCTCAA33 1527 1066072 500818 500837 GTAAGAGTTAGCTATTCCCC 30 1528 1066088501153 501172 CAGTAAAGAGCCACCTAAGG 71 1529 1066104 501574 501593GGCAAGGCTAAGGAGTGCTC 54 1530 1066120 502129 502148 GCTACCCATTAGACCCATGG34 1531 1066136 502502 502521 GGAGTCCCTGTGTCATTGGA 37 1532 1066152502803 502822 GGATGTAGCCCATCAACCCT 77 1533 1066168 503118 503137CCAGTTAATCTCTGACATGG 57 1534 1066184 503422 503441 AGGTGATGACACCCCTACCA57 1535 1066200 503960 503979 GGGTGTCTCTTTGACACCAC 76 1536 1066216504419 504438 AGGCAGTCAGGGTAATGCTA 76 1537 1066232 504667 504686CCTTCTGTCCCAACGGAGCC 111 1538 1066248 505213 505232 CTCTCGCTGAGGACACATCA64 1539 1066264 505791 505810 ACCACAGGGTACTATTCTGG 64 1540 1066280506126 506145 CTGCTCTGTTAGCCGAGCAT 75 1541 1066296 506634 506653GCTTGTATGCCCCACTGGAG 59 1542 1066312 507213 507232 GCTTGCCATGTTTTATAGAC80 1543 1066328 507762 507781 CTCAGGATCGCTGGCCATTT 62 1544 1066344508223 508242 GCCTAAAGGTAGTTCTCCCT 57 1545 1066360 508731 508750CAGGAGGGTGTCAACCAGAC 75 1546 1066376 509178 509197 GGCAGATAACCTCCAAGTGC95 1547 1066392 510128 510147 CACATAGACCATAGCTGAAC 56 1548 1066408510668 510687 GTCTAGTATGTCTGAGTGTC 87 1549 1066424 511065 511084GACTCGGGACATTTAGGATG 72 1550 1066440 511730 511749 ATAGGCCACGCTGGTCACTG48 1551

TABLE 23 Reduction of UBE3A-ATS RNA by 7,000 nM 5-10-5 MOE gapmers with mixed PO/PS internucleoside linkages in vitro (37,000 cells/well) SEQ IDSEQ ID NO: 1 NO: 1 UBE3A- Compound Start Stop ATS SEQ ID  Number SiteSite Sequence (5′ to 3′) (% UTC) NO 617557 483977 483996ATCATGTGCATACCCAGGGT 39 172 749882 460115 460134 ACTTTATAGTGTGGATGGTA 16586 750519 349103# 349122# GTCATCACCTCTCTTCAGGA 14 181 1065260 430471430490 TCATCCTCTATGGACCCACG 83 1552 1065276 432973 432992CCCGACTTACATTACCTACC 69 1553 1065292 441621 441640 ATGATCTGCTGTTCAGTGCG45 1554 1065308 442687 442706 AGGCCAAGTGGTGCTCTTCC 58 1555 1065324443691 443710 ATTCAGATACTAGTTTACCC 36 1556 1065340 445011 445030TCTAGGTAGGCTGAGCCTCA 89 1557 1065356 445972 445991 TTGTCTTGGAACATACGGAT81 1558 1065372 446901 446920 GATTGGCACTATTTTGAGCC 113 1559 1065388447835 447854 GATACTCCAAATATGACCCG 80 1560 1065404 448465 448484AATAGAATAGTGCCAGTAGG 70 1561 1065420 448833 448852 TTGCAAGGACCCTTAAGTCA87 1562 1065436 449328 449347 GTAGCCATAATGCCATAGTC 61 1563 1065452449862 449881 GCATCCTTAAATCCTGGTTG 97 1564 1065468 452186 452205GCAATATCCATAGTGATCTG 84 1565 1065484 453194 453213 CGTTACTGATTATCTTATCC72 1566 1065500 454225 454244 CCCACCATGTTAATCAAGGC 69 1567 1065516454863 454882 CAGGTGTATATTCCCTATCC 70 1568 1065532 455309 455328CCGAATGTGTTATTTATCCT 58 1569 1065548 456621 456640 GGTTATCTAATGCATCATCA71 1570 1065564 456909 456928 CTAGGCACACTAAAGTGCAC 84 1571 1065580457802 457821 GATGCAATCACAGCAAGTAC 68 1572 1065596 458560 458579GTCGAGAACTCAAAAGGTGA 51 1573 1065612 459639 459658 GTTTACCCAGGCAATGGCTC70 1574 1065628 460629 460648 GCAACTCGATGTTCTTGCTT 79 1575 1065643461453 461472 TTGGTAAGGCAGCTCCTGAC 58 1576 1065659 461886 461905TGGTCCCATTGTAGTTGTGT 50 1577 1065674 463502 463521 CTATCAAGGCTGTTATTGGT29 1578 1065689 464477 464496 GCTTCTAACTAACATGCCTC 61 1579 1065704465158 465177 GGGCTCCAGTTTATGTATCC 79 1580 1065720 465418 465437TAGCCTGCATGGTTTACAGT 72 1581 1065736 466030 466049 GGGCTCTTGTTACTGAGCTG79 1582 1065752 466382 466401 TTTCACAGGAGCGCACTTGG 55 1583 1065768467295 467314 GAGTAGTCTATTGGTGTTCC 27 1584 1065784 468404 468423CCCTACCCTTGCATGCTATG 75 1585 1065800 471928 471947 CCATTGAGTATATTACCTCC45 1586 1065816 474789 474808 CCTCCAACTGTAATCATTGA 65 1587 1065832475846 475865 GTAATACGGACCTCATACAG 61 1588 1065848 476316 476335ACTACTTGTCCCCTGGGCTT 45 1589 1065864 479256 479275 CTTGCTTGTATGGTCTGATG63 1590 1065880 480830 480849 AAAAGTAGGTTTGCGGATGC 61 1591 1065896482778 482797 ACCCGTGTACACAGTGGGAT 60 1592 1065912 483896 483915GTCAGTACACAAGCAGGTAG 68 1593 1065927 484309 484328 TCATGCTGGTTACTAGGGCC42 1594 1065943 486378 486397 GTTAGGCCACAAGACTTAAT 51 1595 1065959488046 488065 CTCAAACCCGTATAAAGATG 58 1596 1065975 489527 489546GACATACAATGGACCACCTA 70 1597 1065991 489961 489980 GGAAGTTCCAACCCTTACTC64 1598 1066007 494921 494940 GAATCCACTACATGGGATTA 110 1599 1066023497674 497693 GACTAAGCCCGAAAGTTAGC 79 1600 1066039 499133 499152CGCTTAAAGTAGCCTAAGGA 41 1601 1066055 500400 500419 CGAATACCCTCAACTTCACC59 1602 1066071 500797 500816 CAACCTTGATAGCAGCTTAT 38 1603 1066087501007 501026 GTGAAACTAAGCACATCCGA 77 1604 1066103 501569 501588GGCTAAGGAGTGCTCTTTGT 66 1605 1066119 502125 502144 CCCATTAGACCCATGGCTCA21 1606 1066135 502465 502484 TACAACAGTCCCAGGCTAGG 51 1607 1066151502799 502818 GTAGCCCATCAACCCTGGGA 72 1608 1066167 503107 503126CTGACATGGACTAATAGGCC 47 1609 1066183 503415 503434 GACACCCCTACCATGGCTAC54 1610 1066199 503956 503975 GTCTCTTTGACACCACCAAC 62 1611 1066215504408 504427 GTAATGCTATCTTCCATGCC 47 1612 1066231 504661 504680GTCCCAACGGAGCCTTACGC 62 1613 1066247 505200 505219 CACATCAGCTGTTAGCAGTC64 1614 1066263 505749 505768 ACTCTGTAGGTTGACAGGAC 59 1615 1066279506121 506140 CTGTTAGCCGAGCATTGGCT 88 1616 1066295 506628 506647ATGCCCCACTGGAGAAGTCT 77 1617 1066311 506997 507016 CGTCTTCCCGCACCATGCAT40 1618 1066327 507699 507718 CGTGTCACTTTCAGGTCAGC 59 1619 1066343508216 508235 GGTAGTTCTCCCTTCTGTCA 73 1620 1066359 508726 508745GGGTGTCAACCAGACTTCCA ND 1621 1066375 509128 509147 GCTCATCACAACTGGGTGGT35 1622 1066391 510121 510140 ACCATAGCTGAACCTGTGGC 62 1623 1066407510659 510678 GTCTGAGTGTCAGGCTGTAA 68 1624 1066423 510905 510924GTGTGCCATAGGTTTCAGGC 18 1625 1066439 511725 511744 CCACGCTGGTCACTGAAAGA70 1626

TABLE 24Reduction of UBE3A-ATS RNA by 7,000 nM 5-10-5 MOE gapmers with mixed PO/PSinternucleoside linkages in vitro (37,000 cells/well) SEQ ID SEQ IDUBE3A- Compound NO: 1 NO: 1 Sequence  ATS SEQ ID Number Start SiteStop Site (5′ to 3′) (% UTC) NO 617557 483977 483996ATCATGTGCATACCCAGGGT 49 172 749882 460115 460134 ACTTTATAGTGTGGATGGTA 25586 750519 349103# 349122# GTCATCACCTCTCTTCAGGA 10 181 1065266 432182432201 CCGCTCAATGCAGGACACCA 79 1627 1065282 433495 433514TGCTCCATAGGTAAGATGGT 82 1628 1065298 442149 442168 TAGGCACTGCCTTTGTTGCG87 1629 1065314 442877 442896 GTCGAAGACAATATAACATC 59 1630 1065330444418 444437 ATCAGGGCACATGGAGTTGT 21 1631 1065346 445359 445378CCCTTAGGTGGGAGTCTTCC 128 1632 1065362 446536 446555 GGCTCTAGACCATTGACTCA72 1633 1065378 447498 447517 GTAGTGCCTCAATGATCCAC 62 1634 1065394448042 448061 GGTAAAGTGGACTGGATGCC 66 1635 1065410 448635 448654CTCATGAGGATTGCATCACA 71 1636 1065426 449041 449060 AGGAACGGCCCTGACTGTCA110 1637 1065442 449492 449511 CCATATCAATAACAATTGGC 111 1638 1065458450273 450292 GGAACTCCAGCCAGTGAATA 96 1639 1065474 452270 452289ATGAGGGAAGTCTAGGTCTC 80 1640 1065490 453360 453379 ACATAGGCACTCTACTAGCT101 1641 1065506 454514 454533 TATCAGTCATAGCTACACAC 104 1642 1065522455119 455138 GGTGGATGTCTGACTTGACT 61 1643 1065538 455426 455445GCTAAAGTTGTGGTCTACCA 83 1644 1065554 220324 220343 TTCTCATCATCGATCCAAAC60 1645 456693 456712 1065570 457238 457257 TAATGGCTTAGCTACTCACC 90 16461065586 457994 458013 GAGAACCCACTTGATCTATT 24 1647 1065602 458938 458957GGACCTCAGGAGATTGTACA 35 1648 1065618 460111 460130 TATAGTGTGGATGGTATCCT31 1649 1065634 460744 460763 GTACACAGGGATTTGAGCCT 12* 1650 1065649461528 461547 GGAACATTCAGCTAGACTAG 40 1651 1065665 462567 462586TAAATTGGTCTGGTTACAAG 56 1652 1065679 463779 463798 CCTATAGATTGGGCTTTAGA48 1653 1065695 464905 464924 GTCTATATTTGGTAAGACAC 69 1654 1065710465240 465259 ATTGGTCATCTCGGGTATAT 18 1655 1065726 465596 465615TTCAGACCGAAGGAGTTCCT 60 1656 1065742 466244 466263 GTAGGATCTATGGCAGTTCC59 1657 1065758 466448 466467 GTTCTACAGAGTGGAGCCAG 65 1658 1065774467791 467810 GATTGGTGGTTTATTCATCG 47 1659 1065790 469893 469912TACCCATTGCAAGTTAACTA 73 1660 1065806 473260 473279 TCCTAATAGTTGGAGGTGGTND 1661 1065822 474959 474978 CAGCCTAATATGTGTCATCC 40 1662 1065838476003 476022 CGTTACCTCAATTCTATGGT 49 1663 1065854 478299 478318ACTGGAGTCCTGGTTGATGT 67 1664 1065870 480146 480165 ACAAAACGGGCATGCTTTAT63 1665 1065886 481476 481495 GCGGATAAATTGGATTTATC 37 1666 1065902483108 483127 AGGCAATGGACTTAGTACAC 21 1667 1065918 483965 483984CCCAGGGTAGGATTCATGGT 45 1668 1065933 484683 484702 CAAGTGATGATGTTGTATGC87 1669 1065949 487192 487211 ATCCCCGAATGGGAGAGATT 107 1670 1065965489067 489086 GTGAACGAATACTGTGGCAT 59 1671 1065981 489671 489690CCAACCTGTCATGGGACTGC 45 1672 1065997 493859 493878 TAGGCCTAATCTATGCTGGG44 1673 1066013 496023 496042 TCTCAAAAGACATTCGGTAC 137 1674 1066029498183 498202 CCATGGGCAGATTGATCACC 102 1675 1066045 499515 499534TGCCTAAGGGAGTTTGTCAC 73 1676 1066061 500527 500546 ATGTTTAGAGGTCAAGCCCT91 1677 1066077 500886 500905 TGGGTCGGATATAGCTTTTA 58 1678 1066093501347 501366 GACATCTGCTTATGTAGCTC 45 1679 1066109 501708 501727AGTTACTTTGGGACATCCCA 68 1680 1066125 502209 502228 GTTTGGAACATCATGTTAGC49 1681 1066141 502595 502614 TCCAACTGATGCATCAAGCC 43 1682 1066157502904 502923 GCCTATATCCAACCAGCTAC 73 1683 1066173 503217 503236CGTTGGAAACTTCAAAGGCA 69 1684 1066189 503506 503525 CCTTGGTTGTGGTGAAACCC72 1685 1066205 504087 504106 TATCTTAACTGGTGAACTCC 73 1686 1066221504477 504496 GGATGGTTGACCTCAAAATT 30 1687 1066237 504952 504971TGGTTACCCATACAGTATAT 42 1688 1066253 505549 505568 GCTTTTCAGTCAGGTACAGG29 1689 1066269 505828 505847 GACCTGTGAGGGTGCTTAGT 81 1690 1066285506235 506254 GTTTGACCAGCTCCTTGTTG 76 1691 1066301 506734 506753TTGGGAGTGCTCCACACTTC 72 1692 1066317 507331 507350 GATGCCCTGGTCCTAGCTTC60 1693 1066333 507909 507928 GATAGCCATTCGATACCTGC 56 1694 1066349508297 508316 GCTTCTACATAACCGAAGTG 116 1695 1066365 508933 508952CTAGTGCCTATTACAATCTG 61 1696 1066381 509882 509901 CATGTGCTTTGTGGACCCAT47 1697 1066397 510318 510337 GCTGGACGGACAGTGTTGCT 61 1698 1066413510733 510752 GGTGATGAAGGTCAAGCCGA 103 1699 1066429 511561 511580GTGGTTTCCAGCAGGGTGTA 20 1700 1066445 511777 511796 ACTAAAGAGTACTCCTACCA110 1701

TABLE 25Reduction of UBE3A-ATS RNA by 7,000 5-10-5 MOE gapmers with mixed PO/PSinternucleoside linkages in vitro (37,000 cells/well) SEQ ID SEQ IDUBE3A- Compound NO: 1 NO: 1 ATS SEQ ID Number Start Site Stop SiteSequence (5′ to 3′) (% UTC) NO 617557 483977 483996 ATCATGTGCATACCCAGGGT34 172 749882 460115 460134 ACTTTATAGTGTGGATGGTA 30 586 749921 462626462645 GTAATGATTTGCCCTCCTAC 32 1702 750519 349103# 349122#GTCATCACCTCTCTTCAGGA 17 181 1065267 432208 432227 CCAGCGGGACCTGGACAAGC62 1703 1065283 433699 433718 GGTGTAGTGGCAAGAAGTTC 59 1704 1065299442231 442250 CCCCCTAGCTTTCCAATGGG 82 1705 1065315 442923 442942GTCTCCACATAATCCTATTG 48 1706 1065331 444553 444572 ACATCCCATTAATCATCGCT49 1707 1065347 445388 445407 TCAGATTACCTGAAGGTGTA 104 1708 1065363446582 446601 AGTGGACAGATACGGTCCGT 95 1709 1065379 447508 447527TCAGTTGTGAGTAGTGCCTC 75 1710 1065395 448074 448093 GCTTACCAGAGATGGTTTCC111 1711 1065411 448658 448677 CGTAGGGCAAAAGTAAGGAT 71 1712 1065427449050 449069 GTGGCCTGAAGGAACGGCCC 92 1713 1065443 449532 449551GACCTTCAATTGATCAGTCT 112 1714 1065459 450791 450810 CCTGTCTGGTACTCCCATAT69 1715 1065475 452275 452294 TCAATATGAGGGAAGTCTAG 73 1716 1065491453366 453385 TTAACTACATAGGCACTCTA 76 1717 1065507 454568 454587CTACACCGTGACAAAAGAGC 76 1718 1065523 455125 455144 AGCACAGGTGGATGTCTGAC82 1719 1065539 455450 455469 GTAGTTGTATCTTGAATGGG 65 1720 1065555220380 220399 CATCTGGACCTCAGATTGAC 64 1721 456749 456768 1065571 457288457307 AACGACCTAAAGACCTAGCA 36 1722 1065587 458044 458063CTTGAGTAACTACTCATGAC 83 1723 1065603 458946 458965 GACCTATAGGACCTCAGGAG33 1724 1065619 460118 460137 CTAACTTTATAGTGTGGATG 18 1725 1065635460843 460862 CCACACATATAGGCTAGCCA 9 1726 1065650 461573 461592GGTCATCATATTGAACCAAT 44 1727 1065680 463826 463845 GATTTCCTTACTGAGTGAGC29 1728 1065696 464909 464928 GGTAGTCTATATTTGGTAAG 17 1729 1065711465242 465261 TTATTGGTCATCTCGGGTAT 47 1730 1065727 465600 465619CTATTTCAGACCGAAGGAGT 32 1731 1065743 466251 466270 GTAACTGGTAGGATCTATGG49 1732 1065759 466496 466515 TTTTTAGGGTAGTGTCCTGA 92 1733 1065775467797 467816 CTTAGTGATTGGTGGTTTAT 54 1734 1065791 469899 469918GGGATTTACCCATTGCAAGT ND 1735 1065807 473264 473283 GCCATCCTAATAGTTGGAGG44 1736 1065823 475306 475325 GGTTAAGTCTGCTCTTTCAC 25 1737 1065839476008 476027 TCGCCCGTTACCTCAATTCT 60 1738 1065855 478307 478326GCTATTACACTGGAGTCCTG 45 1739 1065871 480150 480169 CAATACAAAACGGGCATGCT94 1740 1065887 481747 481766 GATCATTCCCTGTGGTAAAG 68 1741 1065903483112 483131 GCTTAGGCAATGGACTTAGT 11 1742 1065919 483969 483988CATACCCAGGGTAGGATTCA 43 1743 1065934 484722 484741 ATAACACTAACGATGAACTC37 1744 1065950 487198 487217 CCTAAAATCCCCGAATGGGA 98 1745 1065966489079 489098 CCCAGTTAATCAGTGAACGA 77 1746 1065982 489676 489695ATGTTCCAACCTGTCATGGG 71 1747 1065998 493867 493886 GATGAGATTAGGCCTAATCT43 1748 1066014 496564 496583 CCATCTACTATTAATGAGCT 58 1749 1066030498186 498205 TCACCATGGGCAGATTGATC 109 1750 1066046 499672 499691GGTAGCCCCAATACAGATTC 26 72 1066062 500564 500583 CAAAACATGTTCTGACCTCG 931751 1066078 500892 500911 GGCCTTTGGGTCGGATATAG 93 1752 1066094 501356501375 CGTCAAACTGACATCTGCTT 38 1753 1066110 501733 501752AGTTAACACCTATCAAGTTG 58 1754 1066126 502355 502374 GGCCACCTGACTTCGGCCCA115 1755 1066142 502599 502618 GACTTCCAACTGATGCATCA 42 1756 1066158502919 502938 GACCCTTCTGTGAAAGCCTA 61 1757 1066174 503279 503298GACTGCATCTCAATCCTATG 62 1758 1066190 503636 503655 GCCCATATGCTTGAACAATT40 1759 1066206 504088 504107 GTATCTTAACTGGTGAACTC 55 1760 1066222504484 504503 GCAGTTTGGATGGTTGACCT 43 1761 1066238 504963 504982GGGTCGACTGATGGTTACCC 80 1762 1066254 505594 505613 ATAGGAGCTGAATAGTAGGG37 1763 1066270 505833 505852 TCAAAGACCTGTGAGGGTGC 78 1764 1066286506243 506262 GGTGAGCTGTTTGACCAGCT 49 1765 1066302 506739 506758TCATTTTGGGAGTGCTCCAC 53 1766 1066318 507359 507378 GGAGATCCAACCTGTGTGGA79 1767 1066334 507915 507934 CTGCAGGATAGCCATTCGAT 69 1768 1066350508354 508373 CATTATTCAATTAAGGGTGG 15 1769 1066366 508999 509018CATGTGTTAACGGAACTGAG 85 1770 1066382 509901 509920 GTGCATAGAGCAGACTGTAC103 1771 1066398 510328 510347 GGTTTTGAGAGCTGGACGGA 57 1772 1066414510740 510759 ATAGGTAGGTGATGAAGGTC 75 1773 1066430 511586 511605GCTGCCAGCTTAGAGAATCT 95 1774 1066446 511781 511800 GTTAACTAAAGAGTACTCCT92 1775

TABLE 26Reduction of UBE3A-ATS RNA by 7,000 nM 5-10-5 MOE gapmers with mixed PO/PSinternucleoside linkages in vitro (37,000 cells/well) SEQ ID SEQ IDNO: 1 NO: 1 UBE3A- Compound Start Stop ATS SEQ ID Number Site  SiteSequence (5′ to 3′) (% UTC) NO 617557 483977 483996 ATCATGTGCATACCCAGGGT35 172 749882 460115 460134 ACTTTATAGTGTGGATGGTA 42 586 749960 465003465022 ATCTGCGAATAGAGGCCCTC 56 33 750519 349103^(#) 349122^(#)GTCATCACCTCTCTTCAGGA 16 181 1065272 432587 432606 GTAGGAGATAGGCAGTGCAT25 1776 1065288 440174 440193 AATTATACTGTCCTACTTCG 65 1777 1065304442560 442579 GTACCCCAGAAATAGCCCAT 45 1778 1065320 443271 443290CATCACTAGCCTAGTGATCT 114 1779 1065336 444826 444845 GGGTGCTGTCAACTGTCCCA45 1780 1065352 445690 445709 GGCAAAGTACATACTGATCC 54 1781 1065368446725 446744 CCTGTGCGGTAGCACTTGCC 80 1782 1065384 447772 447791GGGCCAAGCACATAGGTATC 74 1783 1065400 448306 448325 CGTGCAAAACAAGTTTCGCA91 1784 1065416 448729 448748 GACCACGATAACTGTAGCGC 62 1785 1065432449105 449124 TCCTGGCAAGTATGCTAGTC 73 1786 1065448 449643 449662ACTGATCCATTAGATAGGCT 53 1787 1065464 452074 452093 AGAACACTTCGCCGCTGATT76 1788 1065480 452327 452346 AACATAAGGTTCAAAAGCCG 86 1789 1065496454063 454082 CATAGATGATCCCATAATAG 77 1790 1065512 454746 454765CCGAAAGCACTTTGCATGAG 70 1791 1065528 455227 455246 ATCCTTGATCCAGACAAGTC76 1792 1065544 455606 455625 ATCATACAGGCATCTCAGCC 68 1793 1065560220447 220466 TGTCACTGAGGGATCCCCAA 83 1794 456816 456835 1065576 457383457402 GGATTCAATAGTGTAGGTGA 15 1795 1065592 458407 458426GTACAATGCATGTGGGCCTC ND 1796 1065608 459102 459121 ACTGGCATATTCCGATTATA24 1797 1065624 460467 460486 CGTATTGGACCTTTCAATGA 28 1798 1065640461349 461368 ATTGAAGCTGGCAATATGTC 67 1799 1065655 461608 461627GCATGTACAAACGGGCTATG 33 1800 1065670 463188 463207 GGAATCTTGTAGAGGATTGG51 1801 1065685 464393 464412 TCAGCACTTAGGTAGTTCAC 27 1802 1065716465309 465328 TGTCTTTTGACACGCTGACT 60 1803 1065732 465880 465899GTACTCTTGCCCATGGATGG 42 1804 1065748 466292 466311 CCCCTGTGGCTTATAGGTGG93 1805 1065764 466995 467014 GGATGAACCTGCTTCACACA 45 1806 1065780468051 468070 CAGATTTGCCAGGTAAAGCG 58 1807 1065796 470320 470339AACTATCCTGCATCCGAGGC 33 1808 1065812 474393 474412 GGTCAACCAATTTGCTATTC23 1809 1065828 475722 475741 GGTCTTGGGATTATAGTTTG 32 1810 1065844476155 476174 GGAGTTCCTAACTCTCAATC 78 1811 1065860 478826 478845TCTACTGAGGAACCCATCAC 86 1812 1065876 480715 480734 CGTTGCATAACATGTGTATT46 1813 1065892 482259 482278 TACACCTGCAACAAGCCATC 85 1814 1065908483816 483835 GAGCAAATCGGCAAAGGCAT 43 1815 1065923 484156 484175CCAAGTGGCTTGTGGTGAAA 87 1816 1065939 485817 485836 GAACTACGAATAATTGCATG49 1817 1065955 487610 487629 CAATGGTTTAGTGTCATATG 27 1818 1065971489454 489473 AGTAGCTGTTTAGGCTGGAC 58 1819 1065987 489784 489803TTGCCCTGCCACTCCCGTGG 76 1820 1066003 494419 494438 GGGATATATCACTTGGAGCTND 1821 1066019 496833 496852 CAGATTAGGTATGGAGGCCA 49 1822 1066035498902 498921 ACGGATTAGATTCTCCAACA 36 1823 1066051 499936 499955AGGACACATCAAGCTAGCTA 55 1824 1066067 500680 500699 AAGCCCTTTGCTTTTTCGGG81 1825 1066083 500908 500927 GTGTTTTGACCTAACTGGCC 65 1826 1066099501472 501491 CGTGGGTATGGTTTTCCTCT 75 1827 1066115 501905 501924TCCAACGAGTGATAATTCAC 81 1828 1066131 502423 502442 AATTGATGCATAAGTAAGGC51 1829 1066147 502762 502781 ATAGGCACTTCGGGCAAATA 67 1830 1066163503043 503062 GGATTTAGGTGCTACAGTCA 38 1831 1066179 503356 503375GTTAGCCCAGGTGATCAGCT 57 1832 1066195 503886 503905 ATTGCAACTAATAAAGGGTC79 1833 1066211 504295 504314 GCTCTCATGTGAGAGTATGA 81 1834 1066227504544 504563 TCATGAATTCAGGTTGCCCC 42 1835 1066243 505149 505168CTTTGGTTGGAAGTTAGACC 46 1836 1066259 505666 505685 CTGCTTATTGCAGGCCTAGC69 1837 1066275 506042 506061 CAGTTGAGAACGGTATTGAG 67 1838 1066291506447 506466 GTCTGTCTTTAGGGTCACCC 47 1839 1066307 506828 506847GCTTTAGGATGGTGTGGATC 60 1840 1066323 507587 507606 CTCTCACTGACTAGCTTTCG57 1841 1066339 508114 508133 CTTACCGTCTTTAGGAACTT 47 1842 1066355508585 508604 GGACAGCACAACCCCCGTGC 94 1843 1066371 509070 509089CCCTACAATGCATACCCAAC 58 1844 1066387 510027 510046 GAGTTATACACGGAACCCAC56 1845 1066403 510410 510429 GTCCAAGACATGCTAGGGAC 71 1846 1066419510839 510858 CAATCTAGTTAGACCAGGCA 31 1847 1066435 511667 511686ATGGACTGCCGGCCTGGAGC 49 1848 1066451 511894 511913 GTGAACTACTTGGAGACCTT50 1849

TABLE 27Reduction of UBE3A-ATS RNA by 7,000 nM 5-10-5 MOE gapmers with mixed PO/PSinternucleoside linkages in vitro (37,000 cells/well) SEQ ID SEQ IDNO: 1 NO: 1 Compound Start Stop Sequence UBE3A-ATS SEQ ID Number SiteSite (5′ to 3′) (% UTC) NO 617557 483977 483996 ATCATGTGCATACCCAGGGT 31172 749882 460115 460134 ACTTTATAGTGTGGATGGTA 17 586 750519 349103#349122# GTCATCACCTCTCTTCAGGA 16 181 1065273 432754 432773ACGCAACTGAGACAATCCAT 33 1850 1065289 440229 440248 GTGGCTGTGCTGGATATTAC48 1851 1065305 442574 442593 GAGCAAAATGGTCAGTACCC 43 1852 1065321443357 443376 GATAGGTCAGTCACTCACTG 40 1853 1065337 444867 444886GGATGCATTGGTGTCAAGAG 47 1854 1065353 445763 445782 GGTTTACACATGATAGGAAC80 1855 1065369 446772 446791 AACAACGGTTGCAGGGACAG 29 1856 1065385447787 447806 CCCCGTGTACAGTAAGGGCC 73 1857 1065401 448408 448427GTCTTAGTGCCAAATATCCA 52 1858 1065417 448735 448754 TATTTAGACCACGATAACTG100 1859 1065433 449118 449137 TCCCTCTTGTAGATCCTGGC 40 1860 1065449449666 449685 GAGATATGATTAGTACTGGA 63 1861 1065465 452079 452098GAGATAGAACACTTCGCCGC 39 1862 1065481 453032 453051 GTGATTAGGCGGCCGGGCGC119 1863 1065497 454151 454170 CTTATGGAGACTTATATACC 61 1864 1065513454842 454861 GGGTAGAAGACTAGCATACA 22 1865 1065529 455233 455252GTACTTATCCTTGATCCAGA 48 1866 1065545 455636 455655 AGCACAAGATTGGTTCCATT47 1867 1065561 456827 456846 GGGTACTATGTTGTCACTGA N.D 1868 1065577457571 457590 ATGAACCATGGAGTCTCTAT 77 1869 1065593 458456 458475GGCTTTATACTTTACCCAAC 15 1870 1065609 459265 459284 GTAGATAACTTTACCTTGAC14 1871 1065625 460511 460530 GTTTATAGGGTGGTTGGTTC N.D. 1872 1065641461413 461432 GGGTTACTTTCCAATAGAGT 11 1873 1065656 461610 461629AGGCATGTACAAACGGGCTA 73 1874 1065671 463193 463212 GTTCTGGAATCTTGTAGAGG11 1875 1065686 464398 464417 CCCATTCAGCACTTAGGTAG 23 1876 1065701465011 465030 CTGCTCAAATCTGCGAATAG 73 1877 1065717 465374 465393GAGGATGGTGTGTATGTTAT 31 1878 1065733 465889 465908 TCCCACAAGGTACTCTTGCC61 1879 1065749 466296 466315 GCATCCCCTGTGGCTTATAG 65 1880 1065765467043 467062 TCAGTTAGTCAGGTTAGGGA 7 1881 1065781 468335 468354AGCTATTGCAGCTATGGGTT 35 1882 1065797 470401 470420 GTTCAACCATCGAGATGATC64 1883 1065813 474404 474423 GATAGGGTTTAGGTCAACCA 20 1884 1065829475726 475745 ATCTGGTCTTGGGATTATAG 26 1885 1065845 476159 476178CTTAGGAGTTCCTAACTCTC 56 1886 1065861 478935 478954 GTTTTGGAGATTGTGTTACG57 1887 1065877 480733 480752 GGAGCCAGGTAGAGTTAACG 41 1888 1065893482489 482508 GGATAGTTGTGAACAACTTC 70 1889 1065909 483839 483858CATTGAATGAGGGCCCAAAC 77 1890 1065924 484241 484260 GGACTTGTCTTATGGCTGCCN.D. 1891 1065940 485829 485848 TGATTATTCAGAGAACTACG 47 1892 1065956487697 487716 CGTTGGCATTTAAGTCTGAG 35 1893 1065972 489491 489510CTGGGTTGAGACTATTCAGG 64 1894 1065988 489844 489863 CCATTGAAGAAAAACCCGCG52 1895 1066004 494452 494471 GCACTACTGTAAGGGTCATG 48 1896 1066020497158 497177 ACAAGGGTAGTGTTACACTG 42 1897 1066036 499081 499100CAATGGTAATCGCATATACT 53 1898 1066052 500210 500229 GACTAATCCTCTTAAGTTCA45 1899 1066068 500689 500708 AGGTACTGTAAGCCCTTTGC 80 1900 1066084500972 500991 AACTTTTACACGCTAGTGGG 68 1901 1066100 501490 501509CCCTTTGTTCATACTGAACG 69 1902 1066116 501913 501932 AGCTTCCCTCCAACGAGTGA78 1903 1066132 502433 502452 GGCCCAAGGGAATTGATGCA 45 1904 1066148502766 502785 AGGAATAGGCACTTCGGGCA 58 1905 1066164 503051 503070GGAACCCAGGATTTAGGTGC 50 1906 1066180 503368 503387 GGTCATAGGAGTGTTAGCCC44 1907 1066196 503900 503919 GGTTGGATTGCTTTATTGCA 85 1908 1066212504299 504318 GCCTGCTCTCATGTGAGAGT 47 1909 1066228 504644 504663CGCTTGGCTGACATTTAGGG 38 1910 1066244 505169 505188 ACCGAGCTCAAGAACTGTGA59 1911 1066260 505670 505689 TGGCCTGCTTATTGCAGGCC 73 1912 1066276506047 506066 GTTTTCAGTTGAGAACGGTA 41 1913 1066292 506493 506512GGGACCTCCTTATATTCACC 35 1914 1066308 506833 506852 CCCCTGCTTTAGGATGGTGT134 1915 1066324 507637 507656 GGTCCCCCCAGAAGGCTTGA 56 1916 1066340508118 508137 TATTCTTACCGTCTTTAGGA 39 1917 1066356 508640 508659GGATGGAGCTGTTGTGGCAT 57 1918 1066372 509076 509095 GCATTACCCTACAATGCATA84 1919 1066388 510035 510054 GCATTTGAGAGTTATACACG 33 1920 1066404510596 510615 GGGCCTTATAGACCATACCA 65 1921 1066420 510844 510863GGAGACAATCTAGTTAGACC 30 1922 1066436 511673 511692 GCACAAATGGACTGCCGGCC89 1923 1066452 511964 511983 GAGAGTCATACTCCTGACAG 53 1924

TABLE 28Reduction of UBE3A-ATS RNA by 7,000 nM 5-10-5 MOE gapmers with mixed PO/PSinternucleoside linkages in vitro (37,000 cells/well) SEQ ID SEQ IDUBE3A- Compound NO: 1 NO: 1 Sequence ATS SEQ ID Number Start SiteStop Site (5′ to 3′) (% UTC) NO 617557 483977 483996ATCATGTGCATACCCAGGGT 24 172 749882 460115 460134 ACTTTATAGTGTGGATGGTA 27586 750519 349103# 349122# GTCATCACCTCTCTTCAGGA 7 181 1065262 430574430593 CTCCGGGCTCACCACCTGGT 82 1925 1065278 433033 433052TCTATACACTGCTGTTGACG 52 1926 1065294 441732 441751 GCTTGCTAAACCTGTCCATT40 1927 1065310 442717 442736 CTGGATGGTATAAATTGCCC 43 1928 1065326443811 443830 GTTGCCGCTTTGGTGAACCT 69 1929 1065342 445129 445148GTGCACTGATACAGTTGGGC 31 1930 1065358 446360 446379 CCATCAAGTAGGCCAGTCAC37 1931 1065374 446952 446971 GGAATAACCCATACCCCCCA 97 1932 1065390447883 447902 GCATGACTCCTAATTGCTGT 59 1933 1065406 448539 448558GAGCTCAGTCTTCACTAGTT 46 1934 1065422 448919 448938 GTCAGGCACCAGATTGCTCA70 1935 1065438 449442 449461 ATGATCATACTGGAGCCAGG 8 1936 1065454 449875449894 ACCTACCAGAGTGGCATCCT 47 1937 1065470 452250 452269ACGCTGTGTGAATCAAAAGC 85 1938 1065486 453211 453230 AGTGTTCTTACATCCACCGT62 1939 1065502 454279 454298 CATGCCAATCGCATGCAAGC 71 1940 1065518454967 454986 GTCAGGAGTTAACTATGAAC 95 1941 1065534 455326 455345CACCAGTTAGGCCTGCTCCG 94 1942 1065550 456651 456670 CGTTTCCACAGCCAGGCTTA83 1943 1065566 456921 456940 AGTCTTGGGTCACTAGGCAC 81 1944 1065582457908 457927 CCAATATTTTGACTAGTTCG 23 1945 1065598 458568 458587TTTAGTATGTCGAGAACTCA 31 1946 1065614 459652 459671 TGAAACCTGTATAGTTTACC70 1947 1065630 460652 460671 CTAACCATAGACAGAGTGCA 33 1948 1065645461463 461482 CATCATGATCTTGGTAAGGC 18 1949 1065661 461898 461917GACCCCACAATTTGGTCCCA 62 1950 1065676 463668 463687 GACTATCTCAGCACTAGGGA26 1951 1065691 464558 464577 GCCCTGTAGTATGTGGATAC 46 1952 1065706465231 465250 CTCGGGTATATAAATTAATG 43 1953 1065722 465480 465499GGATCTAGAATGATTGGTTG 75 1954 1065738 466218 466237 GGGCTGCTGTTTGAGTCCCC65 1955 1065754 466397 466416 AGATACTCACTCGCTTTTCA 20 1956 1065770467416 467435 ACGCTCCTTCATTTCATGCA 46 1957 1065786 468925 468944GCTGTAAGTATAGATGCCTC 35 1958 1065802 472661 472680 ATTAGTTGACTCTATAAACG70 1959 1065818 474805 474824 AAGTTTGGTATTGACACCTC 36 1960 1065834475858 475877 CTTGATATAAGGGTAATACG 58 1961 1065850 476393 476412GGCTCAGGCAAAGTAGCTTC 93 1962 1065866 479584 479603 GCCATCTTCAAGACTAAGGT50 1963 1065882 480932 480951 CGAAAAATAGTATGCGAAGC 32 1964 1065898482792 482811 GGAGACCCTAAAAAACCCGT 67 1965 1065914 483913 483932CCTATAGGTGGCCTTCAGTC 30 1966 1065929 484322 484341 GGCAAGCCCAGACTCATGCT86 1967 1065945 486491 486510 CGTCAACACTATAGATGAAT 40 1968 1065961488332 488351 CCACTCATGTACATGAGATC 44 1969 1065977 489547 489566GGCAAGCAACTATGGGTGGA 26 1970 1065993 493198 493217 GTTTACTTCGGGCCAGGTCC97 1971 1066009 495221 495240 ACCCCTAGGAATAATGTTGC 36 1972 1066025497750 497769 AGCGATACAAACCTACGCCA 71 1973 1066041 499146 499165GGCAAGTTTTTATCGCTTAA 48 1974 1066057 500425 500444 AGTATTTTAGCCGGGATACG53 1975 1066073 500846 500865 ACGGGTTCCTATTTGTCAGG 54 1976 1066089501208 501227 GTGAGCTTTACTCTGCAATA 31 1977 1066105 501601 501620GCCTTATTGGAGAGAGAACT 91 1978 1066121 502134 502153 TCAGTGCTACCCATTAGACC35 1979 1066137 502512 502531 CATACATGGAGGAGTCCCTG 59 1980 1066153502809 502828 TCCTAAGGATGTAGCCCATC 55 1981 1066169 503123 503142CGCATCCAGTTAATCTCTGA 84 1982 1066185 503437 503456 GTCCCAGTTGGATTAAGGTG64 1983 1066201 503970 503989 CTGTACAGGAGGGTGTCTCT N.D. 1984 1066217504426 504445 TAGTCTAAGGCAGTCAGGGT 28 1985 1066233 504757 504776ATCTAGACTCTGCCTGGTTA 51 1986 1066249 505218 505237 GGACACTCTCGCTGAGGACA21 1987 1066265 505800 505819 GGCACTATGACCACAGGGTA 35 1988 1066281506127 506146 TCTGCTCTGTTAGCCGAGCA 77 1989 1066297 506641 506660GCACAATGCTTGTATGCCCC 57 1990 1066313 507239 507258 GTGCCCTTACTGTTAAATCC48 1991 1066329 507766 507785 TTTGCTCAGGATCGCTGGCC 93 1992 1066345508230 508249 AGCCTCTGCCTAAAGGTAGT 64 1993 1066361 508780 508799CGTGATTGCAAAGTCCAAGG 47 1994 1066377 509247 509266 CAGTAGTGGTATCAAATCTG36 1995 1066393 510133 510152 TCCAGCACATAGACCATAGC 60 1996 1066409510676 510695 GGTGGAAAGTCTAGTATGTC 73 1997 1066425 511077 511096GACCCATGGAAAGACTCGGG 119 1998 1066441 511737 511756 GTTTCCCATAGGCCACGCTG81 1999

TABLE 29Reduction of UBE3A-ATS RNA by 7,000 nM 5-10-5 MOE gapmers with mixed PO/PSinternucleoside linkages in vitro (37,000 cells/well) SEQ ID SEQ IDNO: 1 NO: 1 UBE3A- Compound Start Stop Sequence ATS SEQ ID Number SiteSite (5′ to 3′) (% UTC) NO 617557 483977 483996 ATCATGTGCATACCCAGGGT 35172 749882 460115 460134 ACTTTATAGTGTGGATGGTA 26 586 749935 463695463714 AGTGGTTGCTATCCTGCTAA 65 2000 750519 349103^(#) 349122^(#)GTCATCACCTCTCTTCAGGA 16 181 1065263 430586 430605 ACGCAAGTCCTCCTCCGGGC73 2001 1065279 433138 433157 TCAAGGGAACTAACTCCTCC 91 2002 1065295441764 441783 GATTGATCATGTTATGCCCT 34 2003 1065311 442754 442773GGTGGATTGGAGTTGCTACA 74 2004 1065327 443823 443842 TGGGACACATATGTTGCCGC81 2005 1065343 445173 445192 CTGAAGCAAAGCTTATAGGC 91 2006 1065359446483 446502 TGACACTGAGTAATCTGCGA 71 2007 1065375 446994 447013TGTAAGATTCCCCCAAGGGT 103 2008 1065391 447907 447926 GGTGGGATTACATCTCTTAT97 2009 1065407 448582 448601 CGTGATATTCATGTTGTCTG 89 2010 1065423448947 448966 CGAAGCCAAAGGTACTTGAG 100 2011 1065439 449461 449480GCTTGCACTCCATCATATAA 126 2012 1065455 449988 450007 AGGACCAGCCAGTCTAGTTT47 2013 1065471 452256 452275 GGTCTCACGCTGTGTGAATC 90 2014 1065487453229 453248 GGAGTCAAGTTGGTTAATAG 136 2015 1065503 454339 454358GGGCGAAAGTATATCAGGCA N.D. 2016 1065519 455012 455031GGCTGTCAATGCTGATATAT 82 2017 1065535 455332 455351 GGAGTTCACCAGTTAGGCCT88 2018 1065551 456669 456688 ACCCTCCCAACAAATAAGCG 160 2019 1065567456925 456944 AGCAAGTCTTGGGTCACTAG 72 2020 1065583 457969 457988GGCATCAATCAACAAGCACC 49 2021 1065599 458570 458589 GCTTTAGTATGTCGAGAACT19 2022 1065615 460105 460124 GTGGATGGTATCCTGGTCAA 46 2023 1065631460660 460679 GCATGATTCTAACCATAGAC 31 2024 1065646 461468 461487GTACTCATCATGATCTTGGT 26 2025 1065662 462030 462049 GGAATAGGGCTCTGCTTATT60 2026 1065692 464776 464795 CCTCGAGGATAGTTTCACTG 76 2027 1065707465234 465253 CATCTCGGGTATATAAATTA 77 2028 1065723 465484 465503TGTTGGATCTAGAATGATTG 71 2029 1065739 466222 466241 GTATGGGCTGCTGTTTGAGT55 2030 1065755 466403 466422 CTGCAGAGATACTCACTCGC 55 2031 1065771467421 467440 TTGATACGCTCCTTCATTTC 75 2032 1065787 468927 468946ATGCTGTAAGTATAGATGCC 86 2033 1065803 472669 472688 CGTTGAATATTAGTTGACTC74 2034 1065819 474875 474894 GGTGTTGTATGATAATGTGT 72 2035 1065835475935 475954 CAATAGTAGTGATGACTTCC 41 2036 1065851 476439 476458AGATGGAGCTTGAGCCATCC 76 2037 1065867 479607 479626 GCCCCACAGTAGAAATGTGG87 2038 1065883 481338 481357 AGTCAACAAGACAACTCGAT 62 2039 1065899482839 482858 CGTTATTCAGTCTCAGGGAG 28 2040 1065915 483919 483938CTTTCACCTATAGGTGGCCT 67 2041 1065930 484442 484461 GAGTAATGGACTTCTGGTCT133 2042 1065946 486772 486791 GTGTGAGGGAATCTAAGATC 54 2043 1065962488566 488585 GGTGCCACACCATCAAAAGA 63 2044 1065978 489581 489600GCTGCAGTGGTACCACAGAC 91 2045 1065994 493203 493222 AAGAAGTTTACTTCGGGCCA59 2046 1066010 495383 495402 TCGGGAGTTGTTAGTCCAAG 62 2047 1066026497873 497892 GCTCCCAAACAACTAATAGT 76 2048 1066042 499204 499223TCAAAATCACGAAAGGCGGG 71 2049 1066058 500430 500449 TTAGAAGTATTTTAGCCGGG44 2050 1066074 500857 500876 GGTAGGTAACTACGGGTTCC 59 2051 1066090501222 501241 CACCCCTGTAAACAGTGAGC 96 2052 1066106 501609 501628CTGCTCAAGCCTTATTGGAG 120 2053 1066122 502140 502159 GTGGAATCAGTGCTACCCAT77 2054 1066138 502527 502546 AGCTGGGCACTCATACATAC 55 2055 1066154502813 502832 AGCCTCCTAAGGATGTAGCC 108 2056 1066170 503131 503150ATGGTCCTCGCATCCAGTTA 54 2057 1066186 503444 503463 CAGCACTGTCCCAGTTGGAT61 2058 1066202 503986 504005 GTTGATTCACTGCACACTGT 80 2059 508752 5087711066218 504433 504452 GGTATATTAGTCTAAGGCAG 43 2060 1066234 504789 504808GCGAAGCCTTCTCAAAGACT 92 2061 1066250 505323 505342 GGGCCGCCTCTGTTATTGTG88 2062 1066266 505808 505827 GAACTGTGGGCACTATGACC 86 2063 1066282506165 506184 CCTTTGGAGCTTTGACTGGC 76 2064 1066298 506677 506696CGATTTCCTGCCAGTGGCTG 79 2065 1066314 507244 507263 CTACTGTGCCCTTACTGTTA83 2066 1066330 507795 507814 GAAGACTTAGGTCTCAAGCT 62 2067 1066346508274 508293 GTAGTCATGTAGAACAGCAC 78 2068 1066362 508784 508803CCACCGTGATTGCAAAGTCC 69 2069 1066378 509252 509271 GGGCACAGTAGTGGTATCAA22 2070 1066394 510203 510222 AGAGCTGTCCTAAGGCAATT 54 2071 1066410510711 510730 CTTGACATACAGGATAGGTG 52 2072 1066426 511088 511107TTGGTAAATTAGACCCATGG 110 2073 1066442 511744 511763 GATGTATGTTTCCCATAGGC56 2074

TABLE 30Reduction of UBE3A-ATS RNA by 8,000 nM 5-10-5 MOE gapmers with mixedPO/PS intemucleoside linkages in vitro (20,000 cells/well) SEQ ID SEQ IDNO: 1 NO: 1 UBE3A- Compound Start Stop ATS SEQ ID Number Site SiteSequence (5′ to 3′) (% UTC) NO 617557 483977 483996 ATCATGTGCATACCCAGGGT51 172 749882 460115 460134 ACTTTATAGTGTGGATGGTA 46 586 750519349103^(#) 349122^(#) GTCATCACCTCTCTTCAGGA 28 181 1165442 449002 449021AGGTCTTATTTACCATTGGC 72 2377 1165447 449444 449463 TAATGATCATACTGGAGCCA87 2378 1165449 452253 452272 CTCACGCTGTGTGAATCAAA 101 2379 1165455452265 452284 GGAAGTCTAGGTCTCACGCT 88 2380 1165467 453362 453381CTACATAGGCACTCTACTAG 96 2381 1165471 454154 454173 ACCCTTATGGAGACTTATAT96 2382 1165477 454461 454480 GACTCCACACACCTACTAGA 97 2383 1165483454578 454597 CAAGCAAAGACTACACCGTG 106 2384 1165487 454848 454867TATCCAGGGTAGAAGACTAG 83 2385 1165493 454861 454880 GGTGTATATTCCCTATCCAG89 2386 1165499 455020 455039 AGTGGGATGGCTGTCAATGC 82 2387 1165510220439 220458 AGGGATCCCCAAATAGAGCC 96 2388 456808 456827 1165516 457018457037 TCACAGTTGCTTGACCCTTA 79 2389 1165522 457572 457591TATGAACCATGGAGTCTCTA 69 2390 1165528 458791 458810 TCCCTAATATAGGGCAGATG88 2391 1165534 458942 458961 TATAGGACCTCAGGAGATTG 52 2392 1165539458950 458969 TCAAGACCTATAGGACCTCA 37 2393 1165546 459550 459569AAAGACTATCCTGGTATGAC 65 2394 1165557 460735 460754 GATTTGAGCCTGCTATGTCT16* 2395 1165565 461525 461544 ACATTCAGCTAGACTAGTTG 58 2396 1165570461901 461920 TGAGACCCCACAATTTGGTC 89 2397 1165572 464385 464404TAGGTAGTTCACAACTCTTC 68 2398 1165582 464562 464581 ATATGCCCTGTAGTATGTGG77 2399 1165587 465245 465264 ATTTTATTGGTCATCTCGGG 47 2400 1165592465387 465406 TGTCAGACTTATTGAGGATG 67 2401 1165594 465891 465910ACTCCCACAAGGTACTCTTG 84 2402 1165598 466249 466268 AACTGGTAGGATCTATGGCA40 2403 1165603 467301 467320 TTCACAGAGTAGTCTATTGG 84 2404 1165609468352 468371 TGTAGTATGCATTGACAAGC 58 2405 1165612 482161 482180GAGTTACAAGTGTCATATAC 48 2406 1165618 483960 483979 GGTAGGATTCATGGTCCAAA64 2407 1165625 485656 485675 CCTAGGACCAGTTGGTTCAC 99 2408 1165633489492 489511 ACTGGGTTGAGACTATTCAG 68 2409 1165638 489521 489540CAATGGACCACCTAAGACCT 104 2410 1165641 493854 493873 CTAATCTATGCTGGGCCCCA89 2411 1165647 493864 493883 GAGATTAGGCCTAATCTATG 116 2412 1165653496830 496849 ATTAGGTATGGAGGCCATGT 69 2413 1165664 499674 499693AAGGTAGCCCCAATACAGAT 87 2414 1165668 500690 500709 GAGGTACTGTAAGCCCTTTG115 2415 1165673 500906 500925 GTTTTGACCTAACTGGCCTT 86 2416 1165678501567 501586 CTAAGGAGTGCTCTTTGTGG 59 2417 1165684 502371 502390AGGTATTAAGGCCCTTGGCC 95 2418 1165690 502378 502397 GTATACAAGGTATTAAGGCC69 2419 1165695 502449 502468 TAGGCAGCTCTTTGTAGGCC 82 2420 1165701503155 503174 AGTTCTGTATACACCATCCC 51 2421 1165707 503361 503380GGAGTGTTAGCCCAGGTGAT 95 2422 1165713 503367 503386 GTCATAGGAGTGTTAGCCCA84 2423 1165719 503421 503440 GGTGATGACACCCCTACCAT 106 2424 1165725503507 503526 GCCTTGGTTGTGGTGAAACC 64 2425 1165729 504076 504095GTGAACTCCTGTGACTGATA 52 2426 1165736 504431 504450 TATATTAGTCTAAGGCAGTC47 2427 1165747 505173 505192 GTGTACCGAGCTCAAGAACT 51 2428 1165753505181 505200 CTGTCTCTGTGTACCGAGCT 64 2429 1165766 505320 505339CCGCCTCTGTTATTGTGATA 78 2430 1165772 505327 505346 AATTGGGCCGCCTCTGTTAT74 2431 1165778 505542 505561 AGTCAGGTACAGGTGTTGGA 56 2432 1165783505745 505764 TGTAGGTTGACAGGACATGC 64 2433 1165789 505821 505840GAGGGTGCTTAGTGAACTGT 87 2434 1165795 505830 505849 AAGACCTGTGAGGGTGCTTA87 2435 1165800 506448 506467 AGTCTGTCTTTAGGGTCACC 57 2436 1165806507907 507926 TAGCCATTCGATACCTGCTT 76 2437 1165812 507914 507933TGCAGGATAGCCATTCGATA 75 2438 1165823 508791 508810 ATGAATGCCACCGTGATTGC94 2439 1165828 510228 510247 AGTTGTGTCTGAGGGATATC 64 2440 1165839448411 448430 AGGGTCTTAGTGCCAAATAT 102 2441 1165852 455763 455782TACCAAAGTGGCTGCTCACC 76 2442 1165854 460426 460445 TAGCCCTTTCAGCACACTTC44 2443 1165856 461342 461361 CTGGCAATATGTCAACCTTA 55 2444 1165864464515 464534 AGTGCACAGATAATGACTAG 91 2445 1165880 484240 484259GACTTGTCTTATGGCTGCCT 90 2446 1165884 486440 486459 CCTAATAGCACCAGAATAGT104 2447 1165891 499666 499685 CCCAATACAGATTCAGTGGC 89 2448 1165901504818 504837 TGAAGTAGTCCTGCCCTTTC 86 2449 1165902 505216 505235ACACTCTCGCTGAGGACACA 74 2450 1165908 506966 506985 GGGCATTCAGTGCTCCCTCC106 2451

TABLE 31Reduction of UBE3A-ATS RNA by 8,000 nM 5-10-5 MOE gapmers with mixed PO/PSinternucleoside linkages in vitro (20,000 cells/well) SEQ ID SEQ IDNO: 1 NO: 1 UBE3A- SEQ Compound Start Stop Sequence ATS ID Number SiteSite (5′ to 3′) (% UTC) NO 617557 483977 483996 ATCATGTGCATACCCAGGGT 53172 749882 460115 460134 ACTTTATAGTGTGGATGGTA 50 586 750519 349103^(#)349122^(#) GTCATCACCTCTCTTCAGGA 31 181 1165439 448413 448432CAAGGGTCTTAGTGCCAAAT 106 2452 1165444 449095 449114 TATGCTAGTCACTCATTGAG113 2453 1165451 452258 452277 TAGGTCTCACGCTGTGTGAA 97 2454 1165457452268 452287 GAGGGAAGTCTAGGTCTCAC 89 2455 1165468 453365 453384TAACTACATAGGCACTCTAC 79 2456 1165473 454161 454180 AGATTTGACCCTTATGGAGA115 2457 1165479 454465 454484 AGTGGACTCCACACACCTAC 108 2458 1165485454843 454862 AGGGTAGAAGACTAGCATAC 96 22 1165489 454857 454876TATATTCCCTATCCAGGGTA 92 2459 1165495 454865 454884 GACAGGTGTATATTCCCTAT116 2460 1165505 456628 456647 TGTGCATGGTTATCTAATGC 74 2461 1165512457013 457032 GTTGCTTGACCCTTAATTCA 108 2462 1165518 457022 457041CATATCACAGTTGCTTGACC 132 2463 1165524 457984 458003 TTGATCTATTATGAGGGCAT22 2464 1165530 458935 458954 CCTCAGGAGATTGTACAACA 47 2465 1165542459545 459564 CTATCCTGGTATGACTGTCA 39 2466 1165548 460110 460129ATAGTGTGGATGGTATCCTG 57 2467 1165553 460508 460527 TATAGGGTGGTTGGTTCAAA29 2468 1165559 460844 460863 ACCACACATATAGGCTAGCC 39 2469 1165566461527 461546 GAACATTCAGCTAGACTAGT 64 2470 1165574 464388 464407ACTTAGGTAGTTCACAACTC 55 2471 1165579 464557 464576 CCCTGTAGTATGTGGATACT97 2472 1165583 465239 465258 TTGGTCATCTCGGGTATATA 43 2473 1165589465380 465399 CTTATTGAGGATGGTGTGTA 64 2474 1165605 467802 467821TCCCTCTTAGTGATTGGTGG 80 2475 1165614 483844 483863 CTAGGCATTGAATGAGGGCC93 2476 1165620 483962 483981 AGGGTAGGATTCATGGTCCA 95 2477 1165627486353 486372 TTAATAGACTGCGATTATAC 100 2478 1165630 489097 489116TCCCTAAGCTTAGATATACC 76 2479 1165634 489516 489535 GACCACCTAAGACCTCAAGG73 2480 1165643 493860 493879 TTAGGCCTAATCTATGCTGG 84 2481 1165649493866 493885 ATGAGATTAGGCCTAATCTA 108 2482 1165655 496832 496851AGATTAGGTATGGAGGCCAT 85 2483 1165660 499668 499687 GCCCCAATACAGATTCAGTG94 2484 1165675 500909 500928 GGTGTTTTGACCTAACTGGC 81 2485 1165680501762 501781 GGCTAAGAGTCACCTGTATC 85 2486 1165686 502373 502392CAAGGTATTAAGGCCCTTGG 92 2487 1165691 502438 502457 TTGTAGGCCCAAGGGAATTG105 2488 1165696 502451 502470 GCTAGGCAGCTCTTTGTAGG 65 2489 1165703503357 503376 TGTTAGCCCAGGTGATCAGC 69 2490 1165709 503363 503382TAGGAGTGTTAGCCCAGGTG 56 2491 1165715 503370 503389 AGGGTCATAGGAGTGTTAGC65 2492 1165721 503424 503443 TAAGGTGATGACACCCCTAC 109 2493 1165726503871 503890 GGGTCAGTTGTGCAGTTGTT 46 2494 1165738 504434 504453AGGTATATTAGTCTAAGGCA 49 2495 1165743 504977 504996 CTATTATCTTCTTAGGGTCG57 2496 1165749 505175 505194 CTGTGTACCGAGCTCAAGAA 65 2497 1165755505198 505217 CATCAGCTGTTAGCAGTCTG 82 2498 1165760 505219 505238AGGACACTCTCGCTGAGGAC 64 2499 1165768 505322 505341 GGCCGCCTCTGTTATTGTGA91 2500 1165774 505329 505348 GAAATTGGGCCGCCTCTGTT 91 2501 1165779505651 505670 CTAGCTTCCACCTAAGAGCT 111 2502 1165785 505750 505769AACTCTGTAGGTTGACAGGA 78 2503 1165791 505824 505843 TGTGAGGGTGCTTAGTGAAC77 2504 1165796 505836 505855 CTGTCAAAGACCTGTGAGGG 78 2505 1165808507910 507929 GGATAGCCATTCGATACCTG 101 2506 1165814 508119 508138TTATTCTTACCGTCTTTAGG 70 2507 1165825 509129 509148 AGCTCATCACAACTGGGTGG86 2508 1165842 449667 449686 AGAGATATGATTAGTACTGG 105 2509 1165850455030 455049 GTGATATTGCAGTGGGATGG 89 2510 1165853 458945 458964ACCTATAGGACCTCAGGAGA 70 2511 1165857 461416 461435 ACTGGGTTACTTTCCAATAG61 2512 1165861 463093 463112 CAATTTACTTGATACAGGGC 43 2513 1165869465393 465412 GTCAATTGTCAGACTTATTG 54 2514 1165873 466100 466119GGGCCTGTATGTCTTGAGAA 88 2515 1165874 467294 467313 AGTAGTCTATTGGTGTTCCT68 2516 1165876 468356 468375 ATACTGTAGTATGCATTGAC 79 2517 1165882484300 484319 TTACTAGGGCCAGAGAATCC 94 2518 1165889 493623 493642CAGATGACTAGCCTCCAAAC 102 2519 1165892 499910 499929 GCATAATAGGAGGTCCTTAA46 2520 1165894 500764 500783 GTCAAATCAATTTGTGCCAC 72 2521 1165900504422 504441 CTAAGGCAGTCAGGGTAATG 55 2522 1165905 506694 506713TTAAGAAGCTTGCCTTTCGA 115 2523 1165909 507901 507920 TTCGATACCTGCTTTTGTGA111 2524 1165914 511254 511273 CTGATGATTTGTTGATTACC 84 2525

TABLE 32Reduction of UBE3A-ATS RNA by 8,000 nM 5-10-5 MOE gapmers with mixed PO/PSinternucleoside linkages in vitro (20,000 cells/well) SEQ ID SEQ IDNO: 1 NO: 1 Compound Start Stop Sequence UBE3A-ATS SEQ ID Number SiteSite (5′ to 3′) (% UTC) NO 617557 483977 483996 ATCATGTGCATACCCAGGGT 49172 749882 460115 460134 ACTTTATAGTGTGGATGGTA 33 586 750519 349103#349122# GTCATCACCTCTCTTCAGGA 22 181 1165440 448459 448478ATAGTGCCAGTAGGACTTAC 98 2526 1165445 449097 449116 AGTATGCTAGTCACTCATTG68 2527 1165452 452259 452278 CTAGGTCTCACGCTGTGTGA 84 2528 1165458452271 452290 TATGAGGGAAGTCTAGGTCT 64 2529 1165474 454162 454181AAGATTTGACCCTTATGGAG 96 2530 1165480 454466 454485 GAGTGGACTCCACACACCTA80 2531 1165490 454858 454877 GTATATTCCCTATCCAGGGT 93 2532 1165496454866 454885 TGACAGGTGTATATTCCCTA 55 2533 1165501 455031 455050AGTGATATTGCAGTGGGATG 77 2534 1165506 220320 220339 CATCATCGATCCAAACAAGC102 2535 456689 456708 1165513 457015 457034 CAGTTGCTTGACCCTTAATT 762536 1165519 457364 457383 ACATGGTGAGGGTCTCAATG 33 2537 1165525 457986458005 ACTTGATCTATTATGAGGGC 35 2538 1165531 458936 458955ACCTCAGGAGATTGTACAAC 42 2539 1165536 458947 458966 AGACCTATAGGACCTCAGGA28 2540 1165543 459546 459565 ACTATCCTGGTATGACTGTC 40 2541 1165549460112 460131 TTATAGTGTGGATGGTATCC 49 2542 1165554 460509 460528TTATAGGGTGGTTGGTTCAA 24 2543 1165560 460845 460864 TACCACACATATAGGCTAGC43 2544 1165567 461671 461690 CCTAAGGTGAAGTCTGTGTA 58 2545 1165575464389 464408 CACTTAGGTAGTTCACAACT 59 2546 1165580 464559 464578TGCCCTGTAGTATGTGGATA 69 2547 1165584 465241 465260 TATTGGTCATCTCGGGTATA51 2548 1165595 466246 466265 TGGTAGGATCTATGGCAGTT 35 2549 1165600467297 467316 CAGAGTAGTCTATTGGTGTT 83 2550 1165606 467803 467822CTCCCTCTTAGTGATTGGTG 84 2551 1165610 468403 468422 CCTACCCTTGCATGCTATGT88 2552 1165615 483850 483869 GCCTGACTAGGCATTGAATG 79 2553 1165622484301 484320 GTTACTAGGGCCAGAGAATC 105 2554 1165628 486354 486373GTTAATAGACTGCGATTATA 43 2555 1165631 489098 489117 CTCCCTAAGCTTAGATATAC88 2556 1165635 489518 489537 TGGACCACCTAAGACCTCAA 73 139 1165644 493861493880 ATTAGGCCTAATCTATGCTG 89 2557 1165650 493868 493887AGATGAGATTAGGCCTAATC 136 2558 1165656 496834 496853 ACAGATTAGGTATGGAGGCC57 2559 1165661 499670 499689 TAGCCCCAATACAGATTCAG 55 2560 1165666499911 499930 TGCATAATAGGAGGTCCTTA 54 2561 1165670 500864 500883TACTTTTGGTAGGTAACTAC 70 2562 1165676 500910 500929 AGGTGTTTTGACCTAACTGG80 2563 1165681 501764 501783 TGGGCTAAGAGTCACCTGTA 109 2564 1165687502374 502393 ACAAGGTATTAAGGCCCTTG 106 2565 1165692 502439 502458TTTGTAGGCCCAAGGGAATT 119 2566 1165697 502740 502759 GCCCGATGACACCAGCCACT61 2567 1165704 503358 503377 GTGTTAGCCCAGGTGATCAG 66 2568 1165710503364 503383 ATAGGAGTGTTAGCCCAGGT 62 2569 1165716 503418 503437GATGACACCCCTACCATGGC 63 2570 1165722 503425 503444 TTAAGGTGATGACACCCCTA102 2571 1165727 503873 503892 AAGGGTCAGTTGTGCAGTTG 43 2572 1165733504427 504446 TTAGTCTAAGGCAGTCAGGG 50 2573 1165739 504435 504454TAGGTATATTAGTCTAAGGC 50 2574 1165744 505167 505186 CGAGCTCAAGAACTGTGACT65 2575 1165750 505176 505195 TCTGTGTACCGAGCTCAAGA 41 2576 1165756505199 505218 ACATCAGCTGTTAGCAGTCT 45 2577 1165761 505220 505239GAGGACACTCTCGCTGAGGA 78 2578 1165769 505324 505343 TGGGCCGCCTCTGTTATTGT79 2579 1165775 505331 505350 TAGAAATTGGGCCGCCTCTG 63 2580 1165780505652 505671 CCTAGCTTCCACCTAAGAGC 78 2581 1165786 505751 505770GAACTCTGTAGGTTGACAGG 43 2582 1165792 505826 505845 CCTGTGAGGGTGCTTAGTGA76 2583 1165797 506166 506185 ACCTTTGGAGCTTTGACTGG 90 2584 1165804507902 507921 ATTCGATACCTGCTTTTGTG 95 2585 1165809 507911 507930AGGATAGCCATTCGATACCT 70 2586 1165837 511896 511915 AAGTGAACTACTTGGAGACC38 2587 1165843 449698 449717 TGTATGGCTTATGCATGCTA 75 2588 1165847453368 453387 TATTAACTACATAGGCACTC 58 2589 1165848 454844 454863CAGGGTAGAAGACTAGCATA 109 2590 1165858 461430 461449 TTAGCACTTCTATAACTGGG40 2591 1165862 463776 463795 ATAGATTGGGCTTTAGAGGT 45 2592 1165867465383 465402 AGACTTATTGAGGATGGTGT 47 2593 1165870 465563 465582TAGGACAAGTCTTATAGAGA 48 2594 1165878 483974 483993 ATGTGCATACCCAGGGTAGG58 2595 1165890 493850 493869 TCTATGCTGGGCCCCAATTC 87 2596 1165906506772 506791 AGGTTGTGGAGGTTGTTCCT 72 2597 1165911 508171 508190ACCTGCAGTTATTTAGCCAT 68 2598 1165912 509171 509190 AACCTCCAAGTGCTTCAAGC60 2599

TABLE 33Reduction of UBE3A-ATS RNA by 8,000 nM 5-10-5 MOE gapmers with mixed PO/PSinternucleoside linkages in vitro (20,000 cells/well) SEQ ID SEQ IDNO: 1 NO: 1 Compound Start Stop UBE3A-ATS SEQ ID Number Site SiteSequence (5′ to 3′) (% UTC) NO 1065438 449442 449461ATGATCATACTGGAGCCAGG 95 1936 1165509 220406 220425 CTTATTTGTCCTATTGGAGG91 2600 456775 456794 1178377 220315 220334 TCGATCCAAACAAGCACCCT 98 2601456684 456703 1178379 220318 220337 TCATCGATCCAAACAAGCAC 132 2602 456687456706 1178384 456753 456772 CGCACATCTGGACCTCAGAT 98 2603 1178386 456755456774 GCCGCACATCTGGACCTCAG 129 2604 1178388 456758 456777AGGGCCGCACATCTGGACCT 115 2605 1178390 456760 456779 GGAGGGCCGCACATCTGGAC70 2606 1178392 456762 456781 TTGGAGGGCCGCACATCTGG 106 2607 1178394456764 456783 TATTGGAGGGCCGCACATCT 123 2608 1178396 456766 456785CCTATTGGAGGGCCGCACAT 136 2609 1178398 456769 456788 TGTCCTATTGGAGGGCCGCA100 2610 1178400 456771 456790 TTTGTCCTATTGGAGGGCCG 104 2611 1178404220455 220474 TACTATGTTGTCACTGAGGG 83 2612 456824 456843 1178406 220457220476 GGTACTATGTTGTCACTGAG 68 2613 456826 456845 1178408 456864 456883TGCCCTCTTCGAAGAGATAG 87 2614 1178411 456914 456933 GGTCACTAGGCACACTAAAG87 2615 1178413 456916 456935 TGGGTCACTAGGCACACTAA 87 2616 1178415456923 456942 CAAGTCTTGGGTCACTAGGC 93 2617 1178417 456926 456945AAGCAAGTCTTGGGTCACTA 72 2618 1178418 457232 457251 CTTAGCTACTCACCCCTGTT111 2619 1178420 457237 457256 AATGGCTTAGCTACTCACCC 79 104 1178422457240 457259 GATAATGGCTTAGCTACTCA 77 2620 1178423 457286 457305CGACCTAAAGACCTAGCAAA 55 2621 1178425 457289 457308 AAACGACCTAAAGACCTAGC50 2622 1178426 457294 457313 GTACAAAACGACCTAAAGAC 77 2623 1178428457574 457593 ATTATGAACCATGGAGTCTC 47 2624 1179735 448291 448310TCGCAATAAGATTCCATTGC 106 2625 1179742 448305 448324 GTGCAAAACAAGTTTCGCAA119 2626 1179745 448453 448472 CCAGTAGGACTTACTAAATC 79 2627 1179747448455 448474 TGCCAGTAGGACTTACTAAA 99 2628 1179749 448457 448476AGTGCCAGTAGGACTTACTA 76 2629 1179751 448630 448649 GAGGATTGCATCACATGTGT118 2630 1179753 448715 448734 TAGCGCATTGAGCAAAATTC 75 2631 1179755448717 448736 TGTAGCGCATTGAGCAAAAT 75 2632 1179757 448719 448738ACTGTAGCGCATTGAGCAAA 83 2633 1179759 448722 448741 ATAACTGTAGCGCATTGAGC103 2634 1179761 448724 448743 CGATAACTGTAGCGCATTGA 68 2635 1179763448727 448746 CCACGATAACTGTAGCGCAT 91 2636 1179765 448730 448749AGACCACGATAACTGTAGCG 84 2637 1179767 448732 448751 TTAGACCACGATAACTGTAG104 2638 1179769 448734 448753 ATTTAGACCACGATAACTGT 114 2639 1179770448737 448756 TATATTTAGACCACGATAAC 106 2640 1179772 448740 448759CTGTATATTTAGACCACGAT 78 2641 1179774 448828 448847 AGGACCCTTAAGTCATAAAG75 2642 1179776 448834 448853 ATTGCAAGGACCCTTAAGTC 75 2643 1179778448841 448860 TATCCCAATTGCAAGGACCC 131 2644 1179781 448948 448967ACGAAGCCAAAGGTACTTGA 124 2645 1179783 449034 449053 GCCCTGACTGTCATTCATAT86 2646 1179785 449037 449056 ACGGCCCTGACTGTCATTCA 98 2647 1179787449039 449058 GAACGGCCCTGACTGTCATT 117 2648 1179789 449042 449061AAGGAACGGCCCTGACTGTC 78 2649 1179791 449044 449063 TGAAGGAACGGCCCTGACTG101 2650 1179793 449046 449065 CCTGAAGGAACGGCCCTGAC 108 2651 1179795449049 449068 TGGCCTGAAGGAACGGCCCT 119 2652 1179797 449052 449071ATGTGGCCTGAAGGAACGGC 76 2653 1179799 449058 449077 ATAATCATGTGGCCTGAAGG104 2654 1179801 449060 449079 CGATAATCATGTGGCCTGAA 79 2655 1179803449063 449082 TAACGATAATCATGTGGCCT 61 2656 1179805 449065 449084CATAACGATAATCATGTGGC 81 2657 1179807 449101 449120 GGCAAGTATGCTAGTCACTC73 2658 1179809 449103 449122 CTGGCAAGTATGCTAGTCAC 105 2659 1179811449106 449125 ATCCTGGCAAGTATGCTAGT 83 2660 1179813 449108 449127AGATCCTGGCAAGTATGCTA 103 2661 1179815 449110 449129 GTAGATCCTGGCAAGTATGC68 2662 1179817 449115 449134 CTCTTGTAGATCCTGGCAAG 78 2663 1179818449157 449176 CAACATTATATCGATGCAAT 89 2664 1179820 449160 449179TGTCAACATTATATCGATGC 109 2665 1179822 449163 449182 ACTTGTCAACATTATATCGA78 2666 1179825 448297 448316 CAAGTTTCGCAATAAGATTC 72 2667 1179833456498 456517 TTAGATTAAAATCTGGCCGG 92 2668 1179834 456912 456931TCACTAGGCACACTAAAGTG 107 2669 1179838 457298 457317 AGATGTACAAAACGACCTAA99 2670 1179840 457386 457405 TAAGGATTCAATAGTGTAGG 34 2671 1179842457503 457522 GGCACACCTGGATTTCACTA 12 2672 1179845 448416 448435GAGCAAGGGTCTTAGTGCCA 108 2673 1179847 448625 448644 TTGCATCACATGTGTCAGTC102 2674 1179849 448843 448862 TTTATCCCAATTGCAAGGAC 86 2675

Example 2 Effect of 5-10-5 MOE Gapmer Modified Oligonucleotides on HumanUBE3A-ATS RNA In Vitro, Single Dose

Modified oligonucleotides complementary to human UBE3A-ATS nucleic acidwere tested for their effect on UBE3A-ATS RNA levels in vitro.

The modified oligonucleotides in the tables below are 5-10-5 gapmers.The gapmers are 20 nucleosides in length, wherein the central gapsegment consists of ten 2′-β-D-deoxynucleosides and the 5′ and 3′ wingsegements each consists of five 2′-MOE nucleosides. The sugar motif forthe gampers is (from 5′ to 3′): eeeeeddddddddddeeeee; wherein representsa 2′-β-D-deoxyribosyl sugar moiety, and ‘e’ represents a 2′-MOE sugarmoiety.

Each internucleoside linkage of Compound IDs 617456, 617457, 617460,617461, and 617557 is a phosphorothioate internucleoside linkage. Allother compounds have an internucleoside linkage motif of:soooossssssssssooss, wherein each “s” represents a phosphorothioateinternucleoside linkage and each “o” represents a phosphodiesterinternucleoside linkage.

All cytosine residues are 5-methylcytosines.

“Start site” indicates the 5′-most nucleoside to which the modifiedoligonucleotide is complementary in target nucleic acid sequence. “Stopsite” indicates the 3′-most nucleoside to which the modifiedoligonucleotide is complementary in the target nucleic acid sequence.Each modified oligonucleotide listed in the Tables below is 100%complementary to SEQ ID NO: 1. Start and stop sites for Compound 750519are marked with a hashtag (#) in several tables below. The complete listof start sites for Compound 750519 are listed in Table 4b.

Human IPS cell derived ReproNeuro™ Neurons (ReproCELL) were cultured permanufacturer instructions at 40,000 cells per well and were treated with8,000 nM of modified oligonucleotide by free uptake. After a treatmentperiod of approximately 5 days, total RNA was isolated from the cellsand UBE3A-ATS RNA levels were measured by quantitative real-time RTPCR.Human UBE3A-ATS primer probe set RTS4796 (described herein in Example 1)was used to measure RNA levels. UBE3A-ATS RNA levels were normalized tototal RNA content, as measured by RIBOGREENO. Reduction of UBE3A-ATS ispresented in the tables below as percent UBE3A-ATS RNA amount relativeto untreated control (UTC) cells. Each table represents results from anindividual assay plate. The values marked with an asterisk (*) indicatethat the modified oligonucleotide is complementary to the ampliconregion of the primer probe set. Additional assays may be used to measurethe potency and efficacy of the modified oligonucleotides complementaryto the amplicon region.

TABLE 34Reduction of UBE3A-ATS RNA by 8,000 nM 5-10-5 MOE gapmers with mixed PO/PSinternucleoside linkages in vitro SEQ ID SEQ ID NO: 1 NO: 1 CompoundStart Stop Sequence UBE3A-ATS SEQ ID Number Site Site (5′ to 3′) (% UTC)NO 617557 483977 483996 ATCATGTGCATACCCAGGGT 38 172 749882 460115 460134ACTTTATAGTGTGGATGGTA 30 586 750519 3491034 3491224 GTCATCACCTCTCTTCAGGA23 181 1165438 448412 448431 AAGGGTCTTAGTGCCAAATA 75 2075 1165443 449094449113 ATGCTAGTCACTCATTGAGA 65 2076 1165448 449641 449660TGATCCATTAGATAGGCTAT 80 2077 1165450 452254 452273 TCTCACGCTGTGTGAATCAA81 2078 1165456 452267 452286 AGGGAAGTCTAGGTCTCACG 54 2079 1165472454155 454174 GACCCTTATGGAGACTTATA 77 2080 1165478 454464 454483GTGGACTCCACACACCTACT 62 2081 1165484 454579 454598 GCAAGCAAAGACTACACCGT48 2082 1165488 454856 454875 ATATTCCCTATCCAGGGTAG 91 2083 1165494454862 454881 AGGTGTATATTCCCTATCCA 76 2084 1165500 455029 455048TGATATTGCAGTGGGATGGC 59 2085 1165504 456625 456644 GCATGGTTATCTAATGCATC60 2086 220440 220459 1165511 456809 456828 GAGGGATCCCCAAATAGAGC 51 20871165517 457019 457038 ATCACAGTTGCTTGACCCTT 63 2088 1165523 457983 458002TGATCTATTATGAGGGCATC 25 2089 1165529 458792 458811 TTCCCTAATATAGGGCAGAT49 2090 1165535 458943 458962 CTATAGGACCTCAGGAGATT 42 2091 1165540458951 458970 CTCAAGACCTATAGGACCTC 50 2092 1165547 460109 460128TAGTGTGGATGGTATCCTGG 55 2093 1165552 460506 460525 TAGGGTGGTTGGTTCAAAAT24 2094 1165558 460842 460861 CACACATATAGGCTAGCCAA 44 2095 1165562461414 461433 TGGGTTACTTTCCAATAGAG 27 2096 1165573 464387 464406CTTAGGTAGTTCACAACTCT 37 2097 1165578 464556 464575 CCTGTAGTATGTGGATACTG40 2098 1165588 465372 465391 GGATGGTGTGTATGTTATGA 32 2099 1165599467293 467312 GTAGTCTATTGGTGTTCCTT 45 2100 1165604 467418 467437ATACGCTCCTTCATTTCATG 49 2101 1165613 483363 483382 GAATTCAATGGACCCACATG67 2102 1165619 483961 483980 GGGTAGGATTCATGGTCCAA 43 2103 1165626486352 486371 TAATAGACTGCGATTATACA 64 2104 1165642 493857 493876GGCCTAATCTATGCTGGGCC 63 2105 1165648 493865 493884 TGAGATTAGGCCTAATCTAT79 2106 1165654 496831 496850 GATTAGGTATGGAGGCCATG 48 2107 1165659499667 499686 CCCCAATACAGATTCAGTGG 75 2108 1165665 499676 499695TCAAGGTAGCCCCAATACAG 44 2109 1165669 500693 500712 CAAGAGGTACTGTAAGCCCT87 2110 1165674 500907 500926 TGTTTTGACCTAACTGGCCT 75 2111 1165679501761 501780 GCTAAGAGTCACCTGTATCC 53 2112 1165685 502372 502391AAGGTATTAAGGCCCTTGGC 54 2113 1165702 503347 503366 GGTGATCAGCTCAACACCCC73 2114 1165708 503362 503381 AGGAGTGTTAGCCCAGGTGA 53 2115 1165714503369 503388 GGGTCATAGGAGTGTTAGCC 77 2116 1165720 503423 503442AAGGTGATGACACCCCTACC 67 2117 1165730 504128 504147 AGCTATTTCATTAAGTCACC43 2118 1165737 504432 504451 GTATATTAGTCTAAGGCAGT 30 2119 1165742504976 504995 TATTATCTTCTTAGGGTCGA 37 2120 1165748 505174 505193TGTGTACCGAGCTCAAGAAC 55 2121 1165754 505184 505203 AGTCTGTCTCTGTGTACCGA70 2122 1165759 505217 505236 GACACTCTCGCTGAGGACAC 58 2123 1165767505321 505340 GCCGCCTCTGTTATTGTGAT 56 2124 1165773 505328 505347AAATTGGGCCGCCTCTGTTA 41 2125 1165784 505746 505765 CTGTAGGTTGACAGGACATG50 2126 1165790 505822 505841 TGAGGGTGCTTAGTGAACTG 78 2127 1165801506449 506468 GAGTCTGTCTTTAGGGTCAC 42 2128 1165803 507899 507918CGATACCTGCTTTTGTGACA 54 2129 1165807 507908 507927 ATAGCCATTCGATACCTGCT56 2130 1165813 507916 507935 ACTGCAGGATAGCCATTCGA 72 2131 1165824508792 508811 TATGAATGCCACCGTGATTG 79 2132 1165846 453364 453383AACTACATAGGCACTCTACT 78 2133 1165859 461526 461545 AACATTCAGCTAGACTAGTT43 2134 1165860 461902 461921 ATGAGACCCCACAATTTGGT 79 2135 1165866464995 465014 ATAGAGGCCCTCTTGTTTCA 60 2136 1165868 465391 465410CAATTGTCAGACTTATTGAG 66 2137 1165872 465893 465912 GAACTCCCACAAGGTACTCT45 2138 1165875 468353 468372 CTGTAGTATGCATTGACAAG 53 2139 1165881484299 484318 TACTAGGGCCAGAGAATCCA 55 2140 1165885 488335 488354AAGCCACTCATGTACATGAG 55 2141 1165887 489494 489513 AAACTGGGTTGAGACTATTC42 2142 1165888 489580 489599 CTGCAGTGGTACCACAGACC 40 2143 1165896502392 502411 TGACTACACATCTTGTATAC 73 2144 1165897 502450 502469CTAGGCAGCTCTTTGTAGGC 25 2145 1165898 503807 503826 CCCTATAGGTCAAAAATGCC51 2146 1165903 505544 505563 TCAGTCAGGTACAGGTGTTG 58 2147 1165904505834 505853 GTCAAAGACCTGTGAGGGTG 63 2148 1165913 510480 510499GGTCTTTGCAGTTAAGTTAT 65 2149

TABLE 35Reduction of UBE3A-ATS RNA by 8,000 nM 5-10-5 MOE gapmers with mixed PO/PSinternucleoside linkages in vitro SEQ ID SEQ ID NO: 1 NO: 1 CompoundStart Stop Sequence UBE3A-ATS SEQ ID Number Site Site (5′ to 3′) (% UTC)NO 617557 483977 483996 ATCATGTGCATACCCAGGGT 52 172 749882 460115 460134ACTTTATAGTGTGGATGGTA 38 586 750519 349103^(#) 349122^(#)GTCATCACCTCTCTTCAGGA 26 181 1165437 448410 448429 GGGTCTTAGTGCCAAATATC112 2150 1165454 452264 452283 GAAGTCTAGGTCTCACGCTG 84 2151 1165466453361 453380 TACATAGGCACTCTACTAGC 96 2152 1165470 454153 454172CCCTTATGGAGACTTATATA 81 2153 1165476 454164 454183 TGAAGATTTGACCCTTATGG114 2154 1165482 454577 454596 AAGCAAAGACTACACCGTGA 80 2155 1165486454847 454866 ATCCAGGGTAGAAGACTAGC 75 2156 1165492 454860 454879GTGTATATTCCCTATCCAGG 83 2157 1165498 455016 455035 GGATGGCTGTCAATGCTGAT84 2158 1165502 455214 455233 ACAAGTCTACTACCAATAAG 57 2159 1165508220325 220344 ATTCTCATCATCGATCCAAA 58 2160 456694 456713 1165515 457017457036 CACAGTTGCTTGACCCTTAA 58 2161 1165521 457382 457401GATTCAATAGTGTAGGTGAC 29 2162 1165527 458789 458808 CCTAATATAGGGCAGATGAT53 2163 1165533 458939 458958 AGGACCTCAGGAGATTGTAC 29 2164 1165538458949 458968 CAAGACCTATAGGACCTCAG 29 2165 1165545 459548 459567AGACTATCCTGGTATGACTG 22 2166 1165551 460353 460372 TGATTGACTACTTCAACCTG64 2167 1165556 460512 460531 CGTTTATAGGGTGGTTGGTT 38 2168 1165564461520 461539 CAGCTAGACTAGTTGAAATC 51 2169 1165569 461900 461919GAGACCCCACAATTTGGTCC 94 2170 1165571 463886 463905 AGGCAGTTGTGATAGTCAAC93 2171 1165577 464397 464416 CCATTCAGCACTTAGGTAGT 29 2172 1165581464561 464580 TATGCCCTGTAGTATGTGGA 56 2173 1165586 465244 465263TTTTATTGGTCATCTCGGGT 27 2174 1165591 465385 465404 TCAGACTTATTGAGGATGGT47 2175 1165597 466248 466267 ACTGGTAGGATCTATGGCAG 43 2176 1165602467299 467318 CACAGAGTAGTCTATTGGTG 62 2177 1165608 467805 467824GTCTCCCTCTTAGTGATTGG 25 2178 1165611 474168 474187 AGTGGTTGCCTTAGTATTAC20 2179 1165617 483858 483877 TGTAAATGGCCTGACTAGGC 75 2180 1165624485655 485674 CTAGGACCAGTTGGTTCACT 72 2181 1165637 489520 489539AATGGACCACCTAAGACCTC 85 2182 1165640 493853 493872 TAATCTATGCTGGGCCCCAA76 2183 1165646 493863 493882 AGATTAGGCCTAATCTATGC 94 2184 1165652496808 496827 GACCCTCATCACTTTTTGAC 66 2185 1165658 498508 498527GCCCGGCAAGAGATTCACTT 79 2186 1165663 499673 499692 AGGTAGCCCCAATACAGATT60 2187 1165667 500493 500512 AGGGCCATGTTAAAGGCCTC 82 2188 1165672500901 500920 GACCTAACTGGCCTTTGGGT 64 2189 1165683 502143 502162TATGTGGAATCAGTGCTACC 75 2190 1165689 502377 502396 TATACAAGGTATTAAGGCCC55 2191 1165694 502446 502465 GCAGCTCTTTGTAGGCCCAA 25 2192 1165700503099 503118 GACTAATAGGCCTTTCTACA 59 2193 1165706 503360 503379GAGTGTTAGCCCAGGTGATC 57 2194 1165712 503366 503385 TCATAGGAGTGTTAGCCCAG36 2195 1165718 503420 503439 GTGATGACACCCCTACCATG 96 2196 1165724503427 503446 GATTAAGGTGATGACACCCC 27 2197 1165728 503943 503962CACCAACCTTAAATAGTAGG 63 2198 1165735 504429 504448 TATTAGTCTAAGGCAGTCAG78 2199 1165741 504653 504672 GGAGCCTTACGCTTGGCTGA 55 2200 1165746505172 505191 TGTACCGAGCTCAAGAACTG 58 2201 1165752 505180 505199TGTCTCTGTGTACCGAGCTC 59 2202 1165758 505215 505234 CACTCTCGCTGAGGACACAT60 2203 1165765 505319 505338 CGCCTCTGTTATTGTGATAT 83 2204 1165771505326 505345 ATTGGGCCGCCTCTGTTATT 54 2205 1165777 505333 505352TGTAGAAATTGGGCCGCCTC 41 2206 1165782 505744 505763 GTAGGTTGACAGGACATGCT75 2207 1165788 505819 505838 GGGTGCTTAGTGAACTGTGG 30 2208 1165794505829 505848 AGACCTGTGAGGGTGCTTAG 84 2209 1165799 506279 506298GTCTACCAGGGTGGTATTAT 70 2210 1165802 506782 506801 TATATACTCCAGGTTGTGGA44 2211 1165805 507906 507925 AGCCATTCGATACCTGCTTT 50 2212 1165811507913 507932 GCAGGATAGCCATTCGATAC 78 2213 1165822 508789 508808GAATGCCACCGTGATTGCAA 51 2214 1165827 510129 510148 GCACATAGACCATAGCTGAA32 2215 1165840 448466 448485 CAATAGAATAGTGCCAGTAG 74 2216 1165841449441 449460 TGATCATACTGGAGCCAGGT 60 2217 1165845 452251 452270CACGCTGTGTGAATCAAAAG 87 2218 1165855 460974 460993 GTGTTTATCCAAACCAAGGG21 2219 1165871 465890 465909 CTCCCACAAGGTACTCTTGC 72 2220 1165879484093 484112 GGATGTCAGTTCAGATGAAC 74 2221 1165883 486393 486412CAACATAGATCCTCTGTTAG 74 2222 1165886 489101 489120 CTGCTCCCTAAGCTTAGATA58 2223 1165895 501152 501171 AGTAAAGAGCCACCTAAGGG 100 2224

TABLE 36Reduction of UBE3A-ATS RNA by 8,000 nM 5-10-5 MOE gapmers with mixed PO/PS internucleoside linkages invitro SEQ ID SEQ ID Compound NO: 1 NO: 1 UBE3A-ATS SEQ ID NumberStart Site Stop Site Sequence (5′ to 3′) (% UTC) NO 617557 483977 483996ATCATGTGCATACCCAGGGT 40 172 749882 460115 460134 ACTTTATAGTGTGGATGGTA 75586 750519 349103^(#) 349122^(#) GTCATCACCTCTCTTCAGGA 28 181 1165436448409 448428 GGTCTTAGTGCCAAATATCC 46 2225 1165441 448460 448479AATAGTGCCAGTAGGACTTA 71 2226 1165446 449099 449118 CAAGTATGCTAGTCACTCAT40 2227 1165453 452260 452279 TCTAGGTCTCACGCTGTGTG 53 2228 1165460453213 453232 ATAGTGTTCTTACATCCACC 59 2229 1165469 454152 454171CCTTATGGAGACTTATATAC 103 2230 1165475 454163 454182 GAAGATTTGACCCTTATGGA91 2231 1165481 454576 454595 AGCAAAGACTACACCGTGAC 69 2232 1165491454859 454878 TGTATATTCCCTATCCAGGG 106 2233 1165497 455015 455034GATGGCTGTCAATGCTGATA 53 2234 1165507 220321 220340 TCATCATCGATCCAAACAAG58 2235 456690 456709 1165514 457016 457035 ACAGTTGCTTGACCCTTAAT 1262236 1165520 457379 457398 TCAATAGTGTAGGTGACATG 63 2237 1165526 458398458417 ATGTGGGCCTCTATTAAGAT 47 2238 1165532 458937 458956GACCTCAGGAGATTGTACAA 45 2239 1165537 458948 458967 AAGACCTATAGGACCTCAGG53 2240 1165544 459547 459566 GACTATCCTGGTATGACTGT 57 2241 1165550460352 460371 GATTGACTACTTCAACCTGA 23 2242 1165555 460510 460529TTTATAGGGTGGTTGGTTCA 30 2243 1165561 460850 460869 GGATTTACCACACATATAGG39 2244 1165563 461451 461470 GGTAAGGCAGCTCCTGACAA 32 2245 1165568461899 461918 AGACCCCACAATTTGGTCCC 51 2246 1165576 464390 464409GCACTTAGGTAGTTCACAAC 41 2247 1165585 465243 465262 TTTATTGGTCATCTCGGGTA44 2248 1165590 465384 465403 CAGACTTATTGAGGATGGTG 30 2249 1165593465564 465583 TTAGGACAAGTCTTATAGAG 20 2250 1165596 466247 466266CTGGTAGGATCTATGGCAGT 32 2251 1165601 467298 467317 ACAGAGTAGTCTATTGGTGT67 2252 1165607 467804 467823 TCTCCCTCTTAGTGATTGGT 45 2253 1165616483851 483870 GGCCTGACTAGGCATTGAAT 16 2254 1165621 483976 483995TCATGTGCATACCCAGGGTA 76 2255 1165623 485654 485673 TAGGACCAGTTGGTTCACTG66 2256 1165629 486356 486375 ATGTTAATAGACTGCGATTA 102 2257 1165632489099 489118 GCTCCCTAAGCTTAGATATA 85 2258 1165636 489519 489538ATGGACCACCTAAGACCTCA 79 2259 1165639 493852 493871 AATCTATGCTGGGCCCCAAT52 2260 1165645 493862 493881 GATTAGGCCTAATCTATGCT 71 2261 1165651496565 496584 TCCATCTACTATTAATGAGC 81 2262 1165657 497266 497285GATTAGGCAGCTTCACTACT 52 2263 1165662 499671 499690 GTAGCCCCAATACAGATTCA35 2264 1165671 500900 500919 ACCTAACTGGCCTTTGGGTC 111 2265 1165677500913 500932 TCAAGGTGTTTTGACCTAAC 64 2266 1165682 501766 501785GATGGGCTAAGAGTCACCTG 99 2267 1165688 502375 502394 TACAAGGTATTAAGGCCCTT98 2268 1165693 502442 502461 CTCTTTGTAGGCCCAAGGGA 66 2269 1165698502763 502782 AATAGGCACTTCGGGCAAAT 75 2270 1165705 503359 503378AGTGTTAGCCCAGGTGATCA 71 2271 1165711 503365 503384 CATAGGAGTGTTAGCCCAGG61 2272 1165717 503419 503438 TGATGACACCCCTACCATGG 96 2273 1165723503426 503445 ATTAAGGTGATGACACCCCT 81 2274 1165734 504428 504447ATTAGTCTAAGGCAGTCAGG 96 2275 1165740 504436 504455 GTAGGTATATTAGTCTAAGG51 2276 1165745 505171 505190 GTACCGAGCTCAAGAACTGT 71 2277 1165751505178 505197 TCTCTGTGTACCGAGCTCAA 58 2278 1165757 505214 505233ACTCTCGCTGAGGACACATC 73 2279 1165762 505223 505242 AATGAGGACACTCTCGCTGA76 2280 1165770 505325 505344 TTGGGCCGCCTCTGTTATTG 51 2281 1165776505332 505351 GTAGAAATTGGGCCGCCTCT 35 2282 1165781 505743 505762TAGGTTGACAGGACATGCTG 66 2283 1165787 505815 505834 GCTTAGTGAACTGTGGGCAC72 2284 1165793 505827 505846 ACCTGTGAGGGTGCTTAGTG 62 2285 1165798506277 506296 CTACCAGGGTGGTATTATAA 33 2286 1165810 507912 507931CAGGATAGCCATTCGATACC 44 2287 1165821 508786 508805 TGCCACCGTGATTGCAAAGT61 2288 1165826 510127 510146 ACATAGACCATAGCTGAACC 65 2289 1165844449863 449882 GGCATCCTTAAATCCTGGTT 66 2290 1165849 454845 454864CCAGGGTAGAAGACTAGCAT 74 2291 1165851 455058 455077 GAACCCTTTGGTCTAAGCAA80 2292 1165863 463777 463796 TATAGATTGGGCTTTAGAGG 76 2293 1165865464560 464579 ATGCCCTGTAGTATGTGGAT 74 2294 1165877 473792 473811GTAATGCTGTTGTACACTAG 59 2295 1165893 499914 499933 TTTTGCATAATAGGAGGTCC62 2296 1165899 503942 503961 ACCAACCTTAAATAGTAGGA 61 2297 1165907506780 506799 TATACTCCAGGTTGTGGAGG 55 2298 1165910 507903 507922CATTCGATACCTGCTTTTGT 71 2299

TABLE 37Reduction of UBE3A-ATS RNA by 8,000 nM 5-10-5 MOE gapmers with mixed PO/PS internucleoside linkagesin vitro SEQ ID SEQ ID UBE3A- Compound NO: 1 NO: 1 ATS SEQ ID NumberStart Site Stop Site Sequence (5′ to 3′) (% UTC) NO 1065438 449442449461 ATGATCATACTGGAGCCAGG 65 1936 1178376 220314 220333CGATCCAAACAAGCACCCTC 80 2300 456683 456702 1178378 220316 220335ATCGATCCAAACAAGCACCC 69 2301 456685 456704 1178380 220319 220338ATCATCGATCCAAACAAGCA 55 2302 456688 456707 1178385 456754 456773CCGCACATCTGGACCTCAGA 97 2303 1178387 456756 456775 GGCCGCACATCTGGACCTCA89 2304 1178389 456759 456778 GAGGGCCGCACATCTGGACC 94 2305 1178391456761 456780 TGGAGGGCCGCACATCTGGA 83 2306 1178393 456763 456782ATTGGAGGGCCGCACATCTG 93 2307 1178395 456765 456784 CTATTGGAGGGCCGCACATC63 2308 1178397 456767 456786 TCCTATTGGAGGGCCGCACA 90 2309 1178399456770 456789 TTGTCCTATTGGAGGGCCGC 64 2310 1178401 220403 220422ATTTGTCCTATTGGAGGGCC 101 2311 456772 456791 1178403 220452 220471TATGTTGTCACTGAGGGATC 78 2312 456821 456840 1178405 220456 220475GTACTATGTTGTCACTGAGG 80 2313 456825 456844 1178407 456863 456882GCCCTCTTCGAAGAGATAGA 90 2314 1178409 456865 456884 CTGCCCTCTTCGAAGAGATA58 2315 1178410 456913 456932 GTCACTAGGCACACTAAAGT 62 2316 1178412456915 456934 GGGTCACTAGGCACACTAAA 76 2317 1178414 456922 456941AAGTCTTGGGTCACTAGGCA 63 2318 1178416 456924 456943 GCAAGTCTTGGGTCACTAGG81 2319 1178419 457236 457255 ATGGCTTAGCTACTCACCCC 68 2320 1178421457239 457258 ATAATGGCTTAGCTACTCAC 60 2321 1178424 457287 457306ACGACCTAAAGACCTAGCAA 49 2322 1178427 457297 457316 GATGTACAAAACGACCTAAA60 2323 1179741 448298 448317 ACAAGTTTCGCAATAAGATT 55 2324 1179743448414 448433 GCAAGGGTCTTAGTGCCAAA 78 2325 1179744 448450 448469GTAGGACTTACTAAATCATC 75 2326 1179746 448454 448473 GCCAGTAGGACTTACTAAAT57 2327 1179748 448456 448475 GTGCCAGTAGGACTTACTAA 62 2328 1179750448629 448648 AGGATTGCATCACATGTGTC 56 2329 1179752 448714 448733AGCGCATTGAGCAAAATTCC 82 2330 1179754 448716 448735 GTAGCGCATTGAGCAAAATT76 2331 1179756 448718 448737 CTGTAGCGCATTGAGCAAAA 83 2332 1179758448720 448739 AACTGTAGCGCATTGAGCAA 52 2333 1179760 448723 448742GATAACTGTAGCGCATTGAG 85 2334 1179762 448726 448745 CACGATAACTGTAGCGCATT38 2335 1179764 448728 448747 ACCACGATAACTGTAGCGCA 67 2336 1179766448731 448750 TAGACCACGATAACTGTAGC 80 2337 1179768 448733 448752TTTAGACCACGATAACTGTA 54 2338 1179771 448738 448757 GTATATTTAGACCACGATAA58 2339 1179773 448741 448760 TCTGTATATTTAGACCACGA 49 2340 1179775448831 448850 GCAAGGACCCTTAAGTCATA 72 2341 1179777 448836 448855CAATTGCAAGGACCCTTAAG 99 2342 1179779 448842 448861 TTATCCCAATTGCAAGGACC49 2343 1179780 448920 448939 AGTCAGGCACCAGATTGCTC 112 2344 1179782448949 448968 AACGAAGCCAAAGGTACTTG 80 2345 1179784 449036 449055CGGCCCTGACTGTCATTCAT 71 2346 1179786 449038 449057 AACGGCCCTGACTGTCATTC53 2347 1179788 449040 449059 GGAACGGCCCTGACTGTCAT 95 2348 1179790449043 449062 GAAGGAACGGCCCTGACTGT 70 2349 1179792 449045 449064CTGAAGGAACGGCCCTGACT 105 2350 1179794 449047 449066 GCCTGAAGGAACGGCCCTGA111 2351 1179796 449051 449070 TGTGGCCTGAAGGAACGGCC 100 2352 1179798449054 449073 TCATGTGGCCTGAAGGAACG 68 2353 1179800 449059 449078GATAATCATGTGGCCTGAAG 49 2354 1179802 449062 449081 AACGATAATCATGTGGCCTG86 2355 1179804 449064 449083 ATAACGATAATCATGTGGCC 52 2356 1179806449096 449115 GTATGCTAGTCACTCATTGA 91 2357 1179808 449102 449121TGGCAAGTATGCTAGTCACT 12 2358 1179810 449104 449123 CCTGGCAAGTATGCTAGTCA76 2359 1179812 449107 449126 GATCCTGGCAAGTATGCTAG 103 2360 1179814449109 449128 TAGATCCTGGCAAGTATGCT 71 2361 1179816 449111 449130TGTAGATCCTGGCAAGTATG 79 2362 1179819 449158 449177 TCAACATTATATCGATGCAA66 2363 1179821 449162 449181 CTTGTCAACATTATATCGAT 61 2364 1179823449164 449183 AACTTGTCAACATTATATCG 79 2365 1179824 448294 448313GTTTCGCAATAAGATTCCAT 98 2366 1179835 457231 457250 TTAGCTACTCACCCCTGTTC74 2367 1179836 457241 457260 AGATAATGGCTTAGCTACTC 63 2368 1179837457290 457309 AAAACGACCTAAAGACCTAG 92 2369 1179839 457384 457403AGGATTCAATAGTGTAGGTG 25 2370 1179841 457502 457521 GCACACCTGGATTTCACTAC33 2371 1179843 457505 457524 TTGGCACACCTGGATTTCAC 28 2372 1179844457674 457693 ATAACTTACCTTGTTGCAAC 65 2373 1179846 448600 448619TTGAGAAGAAAACCCTATCG 82 2374 1179848 448736 448755 ATATTTAGACCACGATAACT83 2375 1179850 449117 449136 CCCTCTTGTAGATCCTGGCA 51 2376

Example 3 Effect of 5-10-5 MOE Gapmer Modified Oligonucleotides on HumanUBE3A-ATS RNA In Vitro, Single Dose

Modified oligonucleotides complementary to human UBE3A-ATS nucleic acidwere tested for their effect on UBE3A-ATS RNA levels in vitro,essentially as described in Example 1, except that human UBE3A-ATSprimer probe set LTS01075 (forward sequence GCCCGAAGTGCCTATTCCTT,designated herein as SEQ ID NO: 5; reverse sequenceTGGTCAGGAGAACATAGGCATAAA, designated herein as SEQ ID NO: 6; probesequence ACTCCCAGGGTTGATGGGCTACATCC, designated herein as SEQ ID NO: 7)was used to measure RNA levels.

The modified oligonucleotides in the tables below are 5-10-5 gapmers.The gapmers are 20 nucleosides in length, wherein the central gapsegment consists of ten 2′-β-D-deoxynucleosides and the 3′ and 5′ wingsegements each consists of five 2′-MOE nucleosides. The sugar motif forthe gapmer is (from 5′ to 3′): eeeeeddddddddddeeeee; wherein representsa 2′-β-D-deoxyribosyl sugar moiety, and ‘e’ represents a 2′-MOE sugarmoiety. Each internucleoside linkage of Compound IDs 617456, 617457,617460, 617461, and 617557 is a phosphorothioate internucleosidelinkage. All other compounds have an internucleoside linkage motif of:soooossssssssssooss, wherein each “s” represents a phosphorothioateinternucleoside linkage and each “o” represents a phosphodiesterinternucleoside linkage.

All cytosine residues are 5-methylcytosines.

“Start site” indicates the 5′-most nucleoside to which the modifiedoligonucleotide is complementary in the target nucleic acid. “Stop site”indicates the 3′-most nucleoside to which the modified oligonucleotideis complementary in the target nucleic acid. Each modifiedoligonucleotide listed in the Tables below is 100% complementary to SEQID NO: 1.

Reduction of UBE3A-ATS RNA is presented in the tables below as percentUBE3A-ATS RNA amount relative to untreated control (UTC) cells. Thevalues marked with an asterisk (*) indicate that the modifiedoligonucleotide is complementary to the amplicon region of the primerprobe set. Additional assays may be used to measure the potency andefficacy of the modified oligonucleotides complementary to the ampliconregion.

TABLE 38Reduction of UBE3A-ATS RNA by 7,000 nM 5-10-5 MOE gapmers with PS internucleoside linkages in vitro(35,000 cells/well) SEQ ID SEQ ID UBE3A- Compound NO: 1 NO: 1 ATS SEQ IDNumber Start Site Stop Site Sequence (5′ to 3′) (% UTC) NO 617456 463905463924 CAACTGTTACCAAGACTTCA 25 32 617457 465003 465022ATCTGCGAATAGAGGCCCTC 14 33 617460 466539 466558 TAGGTTGCATAAAGCCAGGC 2236 617461 466981 467000 CACACATCTTGTTCCCTCAA 15 37 617557 483977 483996ATCATGTGCATACCCAGGGT 17 172 699779 463905 463924 CAACTGTTACCAAGACTTCA 4232 749897 461087 461106 AATAATATTACTGCCAAATG 85 2676 749898 461222461241 TGAATCAATTTTCAATATTT 104 2677 749899 461325 461344TTATAAACTTCAGCAGAGTC 41 2678 749900 461397 461416 GAGTTGTTATTTGCATTAAG44 2679 749901 461431 461450 ATTAGCACTTCTATAACTGG 49 2680 749902 461442461461 GCTCCTGACAAATTAGCACT 91 1329 749903 461455 461474TCTTGGTAAGGCAGCTCCTG 64 2681 749904 461467 461486 TACTCATCATGATCTTGGTA54 2682 749905 461516 461535 TAGACTAGTTGAAATCGGAA 46 2683 749906 461556461575 AATTCTAAAAGTCCTCTCTT 69 2684 749907 461590 461609TGTGAGATAATTCAGGAGGT 11 2685 749908 461810 461829 ATTCACTTTATAAACACTGA68 2686 749909 461895 461914 CCCACAATTTGGTCCCATTG 39 2687 749910 462029462048 GAATAGGGCTCTGCTTATTT 57 2688 749911 462064 462083TTTTATGGCCCTCCCATCAG 81 2689 749912 462098 462117 CATGAATTTAATTCTTTAAA80 2690 749913 462126 462145 CATTGTGGAATTAAATTAAC 54 2691 749914 462141462160 TTTCTAATTCAATATCATTG 59 2692 749915 462149 462168TCCTCTTATTTCTAATTCAA 54 2693 749916 462159 462178 CAAGAGATATTCCTCTTATT41 2694 749917 462212 462231 CAATAAATAGGTCAGAAATG 84 2695 749918 462406462425 CTAAGTTTCTTAAGGTAAAA 65 2696 749919 462607 462626CATTTTCAAATATTGGTATT 77 2697 749920 462625 462644 TAATGATTTGCCCTCCTACA51 2698 749921 462626 462645 GTAATGATTTGCCCTCCTAC 26 1702 749922 462674462693 CCTTTTAAATAATTTTTCCT 66 2699 749923 462992 463011AAAATGTTGGCATACATTTT 70 2700 749924 462993 463012 AAAAATGTTGGCATACATTT69 2701 749925 463248 463267 CCTGGGTATTGCTGTCCAAA 36 2702 749926 463307463326 TATGTTCCTAAGGAATAATG 113 2703 749927 463318 463337TTCTTTGCATTTATGTTCCT 40 2704 749928 463319 463338 TTTCTTTGCATTTATGTTCC36 2705 749929 463453 463472 TTACTCTGACTTTCCAGAAG 70 2706 749930 463474463493 GGAGTAGATTTTTGGAGTTT 43 2707 749931 463512 463531TCAACTATTTCTATCAAGGC 22 2708 749932 463519 463538 ATTAATTTCAACTATTTCTA87 2709 749933 463601 463620 TCTTACTGATTCAGCCATTT 27 2710 749934 463663463682 TCTCAGCACTAGGGAGAAAA 56 2711 749935 463695 463714AGTGGTTGCTATCCTGCTAA 71 2000 749936 463788 463807 AATATAAATCCTATAGATTG71 2712 749937 463805 463824 CTGAGTCAGTCCAAATGAAT 40 2713 749938 463840463859 TACCTTGAAATTGAGATTTC 50 2714 749939 463853 463872TCTTTTTGACCAATACCTTG 34 2715 749940 463871 463890 TCAACAATTGCCATGGATTC78 2716 749941 463895 463914 CAAGACTTCAGGCAGTTGTG 54 2717 749942 463898463917 TACCAAGACTTCAGGCAGTT 51 2718 749943 463900 463919GTTACCAAGACTTCAGGCAG 35 2719 749944 463903 463922 ACTGTTACCAAGACTTCAGG37 2720 749945 463907 463926 CCCAACTGTTACCAAGACTT 27 2721 749946 463910463929 TATCCCAACTGTTACCAAGA 37 2722 749947 463912 463931TTTATCCCAACTGTTACCAA 29 2723 749948 463915 463934 TTGTTTATCCCAACTGTTAC42 2724 749949 463926 463945 TGATCAGCTTCTTGTTTATC 29 2725 749950 464355464374 CCAGAGCATAAAAGGAAAGC 87 2726 749951 464377 464396TCACAACTCTTCCTTAGCTT 28 2727 749952 464525 464544 CAGTTAGGTTAGTGCACAGA44 2728 749953 464530 464549 CTGCTCAGTTAGGTTAGTGC 31 2729 749954 464542464561 ATACTGAAGTCTCTGCTCAG 53 2730 749955 464738 464757TGTGCCATATTTTTCTATTT 41 2731 749956 464993 465012 AGAGGCCCTCTTGTTTCAAT36 2732 749957 464996 465015 AATAGAGGCCCTCTTGTTTC 42 2733 749958 464998465017 CGAATAGAGGCCCTCTTGTT 59 2734 749959 465001 465020CTGCGAATAGAGGCCCTCTT 35 2735 749960 465003 465022 ATCTGCGAATAGAGGCCCTC30 33 749961 465005 465024 AAATCTGCGAATAGAGGCCC 33 2736 749962 465008465027 CTCAAATCTGCGAATAGAGG 34 2737 749963 465010 465029TGCTCAAATCTGCGAATAGA 67 2738 749964 465013 465032 GCCTGCTCAAATCTGCGAAT24 2739 749965 465050 465069 AGTTGACATATCTTCAAGTT 69 2740 749966 465057465076 TAATCTCAGTTGACATATCT 55 2741 749967 465059 465078GATAATCTCAGTTGACATAT 42 2742

Example 4 Effect of Modified Oligonucleotides on Human UBE3A-ATS RNA InVitro, Multiple Doses

Modified oligonucleotides selected from the examples above were testedat various doses in human IPS derived iCell GABANeurons (CellularDynamics). Cells were plated at a density of 35,000-60,000 cells perwell, maintained per manufacturer instructions, and treated withmodified oligonucleotides by free uptake at various concentrations asspecified in the tables below. After a treatment period of approximately6 days, total RNA was isolated from the cells and UBE3A-ATS RNA levelswere measured by quantitative real-time PCR. Human UBE3A-ATS primerprobe set RTS4796, described hereinabove, was used to measure RNAlevels. UBE3A-ATS RNA levels were normalized according to total RNAcontent, as measured by RIBOGREEN®. Reduction of UBE3A-ATS RNA ispresented in the tables below as percent UBE3A-ATS RNA amount relativeto untreated control (UTC) cells.

Where possible, the half maximal inhibitory concentration (IC_(so)) ofeach modified oligonucleotide was calculated using a linear regressionon a log/linear plot of the data in excel. In some cases, an IC₅₀ couldnot be reliably calculated and the data point is marked as “N.C.”.Values marked with “N.D.” indicate that a value was not determined inthis experiment.

TABLE 39 Dose-dependent percent reduction of human UBE3A-ATS RNA invitro (60,000 cells/well) Compound UBE3A-ATS expression (% UTC) Number740.7 nM 2,222 nM 6,666 nM 20,000 nM 617456 43 35 16 11 617457 38 32 2718 617460 28 29 24 15 617461 40 19 11 12 617557 23 18 20 16

TABLE 40 Dose-dependent percent reduction of human UBE3A-ATS RNA invitro (60,000 cells/well) Compound UBE3A-ATS expression (% UTC) Number740.7 nM 2,222 nM 6,666 nM 20,000 nM 617456 48 38 17 15 617457 35 27 1818 617460 29 31 24 18 617461 38 24 13 12 617557 23 17 14 13

TABLE 41 Dose-dependent percent reduction of human UBE3A-ATS RNA invitro (60,000 cells/well) Compound UBE3A-ATS expression (% UTC) IC₅₀Number 740.7 nM 2,222 nM 6,666 nM 20,000 nM (μM) 617456 41 26 15 20 N.C.617557 69 43 24 30 1.9 749969 46 15 12 5 N.C. 749991 43 27 19 13 N.C.750028 54 31 24 15 N.C. 750030 57 37 23 14 1.0 750032 40 18 14 7 N.C.750326 76 60 38 25 3.8 750329 60 57 33 16 2.1 750344 63 77 49 29 5.6750350 67 71 59 35 8.6 750359 110 56 38 41 6.7 750360 69 47 39 38 3.3750365 58 60 38 22 2.4 750366 87 58 44 24 4.6 750386 76 57 42 40 5.3750517 30 25 12 8 N.C. 750519 45 23 10 8 N.C. 750542 96 71 55 41 9.9750549 92 54 40 24 4.3

TABLE 42 Dose-dependent percent reduction of human UBE3A-ATS RNA invitro (60,000 cells/well) Compound UBE3A-ATS expression (% UTC) IC₅₀Number 740.7 nM 2,222 nM 6,666 nM 20,000 nM (μM) 617557 85 57 37 25 4.1749861 47 31 25 14 N.C. 749863 83 52 34 23 3.5 749869 46 30 18 11 N.C.749882 84 60 40 27 4.5 749885 61 53 31 20 2.0 749893 63 36 25 13 1.3749894 28 21 10 11 N.C. 750040 78 59 46 32 5.1 750051 47 18 21 23 N.C.750092 80 54 40 28 3.9 750100 82 67 59 51 N.C. 750270 43 34 25 21 N.C.750292 58 37 28 26 1.0 750312 57 54 41 30 2.3 750325 101 94 68 46 18.4 750413 98 60 35 28 4.9 750416 94 48 38 15 3.7 750430 85 47 33 24 3.3750431 136 125 90 62 N.C.

TABLE 43 Dose-dependent percent reduction of human UBE3A-ATS RNA invitro (35,000 cells/well) Compound UBE3A-ATS expression (% UTC) IC₅₀Number 740.7 nM 2,222 nM 6,666 nM 20,000 nM (μM) 617459 118 69 33 20 5.4617470 84 51 28 13 2.9 617473 44 33 30 20 N.C. 617536 100 54 57 24 5.9617547 122 52 29 18 4.6 617593 81 75 32 21 4.2 749794 89 102 66 52 N.C.750418 135 132 105 51 N.C. 750439 98 60 44 26 5.4 750452 113 94 78 51N.C.

TABLE 44 Dose-dependent percent reduction of human UBE3A-ATS RNA invitro (35,000 cells/well) Compound UBE3A-ATS expression (% UTC) IC₅₀Number 740.7 nM 2,222 nM 6,666 nM 20,000 nM (μM) 617557 129 53 32 28 5.5749796 84 31 29 17 2.3 749816 94 56 46 22 4.8 749907 65 57 21 16 2.1749921 50 47 25 18 1.0 749931 246 39 32 12 6.3 749933 51 41 26 11 0.9749937 74 46 27 16 2.3 749944 116 N/A 74 40 15.3  749956 136 74 18 275.7 749964 75 80 35 17 4.2 750131 73 50 36 25 2.9 750139 35 29 21 15N.C. 750140 27 27 13 11 N.C. 750141 52 61 25 24 1.6 750196 107 95 129 47N.C. 750210 53 38 26 23 0.8 750214 34 70 47 41 N.C. 750228 109 105  9570 N.C. 750242 77 70 36 15 3.8

TABLE 45 Dose-dependent percent reduction of human UBE3A-ATS RNA invitro (35,000 cells/well) Compound UBE3A-ATS expression (% UTC) IC₅₀Number 740.7 nM 2,222 nM 6,666 nM 20,000 nM (μM) 617557 80 59 31 13 3.1750519 87 67 41 27 5.2 1065438 120 90 88 95 N.C. 1065582 65 61 41 29 3.41065597 142 135 111 103 N.C. 1065599 63 47 47 26 2.5 1065613 96 70 65 3911.5  1065631 60 61 48 26 3.4 1065644 64 65 42 27 3.8 1065645 68 50 3619 2.4 1065646 52 55 34 16 1.5 1065676 50 38 33 23 N.C. 1065690 56 53 3824 1.9 1065754 74 56 57 27 5.1 1065817 94 76 42 38 7.6 1065899 59 56 4630 3.0 1066072 133 108 86 63 N.C. 1066249 73 60 70 48 N.C. 1066378 116114 87 52 N.C.

TABLE 46 Dose-dependent percent reduction of human UBE3A-ATS RNA bymodified oligonucleotides in vitro (42,000 cells/well) CompoundUBE3A-ATS expression (% UTC) IC₅₀ Number 740.7 nM 2,222 nM 6,666 nM20,000 nM (μM) 617557 83 54 31 13 3.1 750519 63 48 43 26 2.4 1065578 7736 36 24 2.4 1065579 55 58 30 66 N.C. 1065595 75 60 32 19 3.1 1065642 5258 40 27 2.0 1065672 85 67 42 18 4.5 1065674 64 69 40 22 3.5 1065719 7662 46 38 6.1 1065750 38 28 25 28 N.C. 1065766 78 52 32 37 3.7 1065768 8666 43 30 5.5 1065799 66 52 41 34 3.2 1065863 54 60 46 27 2.6 1065894 82100 52 52 N.C. 1066037 66 70 56 62 N.C. 1066119 82 116 101 72 N.C.1066375 116 98 68 77 N.C. 1066423 39 70 63 46 N.C.

TABLE 47 Dose-dependent percent reduction of human UBE3A-ATS RNA invitro (42,000 cells/well) Compound UBE3A-ATS expression (% UTC) IC₅₀Number 740.7 nM 2,222 nM 6,666 nM 20,000 nM (μM) 617557 65 52 23 8 1.91065324 86 90 67 43 18.4  1065369 107 91 108 83 N.C. 1065465 97 96 97100 N.C. 1065513 156 104 66 70 N.C. 1065558 116 125 92 112 N.C. 106559285 84 50 23 6.5 1065624 87 71 39 20 4.7 1065667 64 59 35 21 2.7 106574791 65 55 N/A N.C. 1065955 84 55 43 23 4.1 1066002 94 75 68 50 N.C.1066003 90 73 49 43 9.5 1066034 137 120 101 67 N.C. 1066201 98 110 84 79N.C. 1066273 101 95 108 104 N.C. 1066359 111 96 101 81 N.C. 1066420 7043 27 33 2.2

TABLE 48 Dose-dependent percent reduction of human UBE3A-ATS RNA invitro (42,000 cells/well) Compound UBE3A-ATS expression (% UTC) IC₅₀Number 740.7 nM 2,222 nM 6,666 nM 20,000 nM (μM) 617557 70 46 23 10 1.91065654 96 87 54 43 11.9  1065680 78 63 41 28 4.5 1065686 63 64 62 245.1 1065735 102 66 41 41 7.4 1065785 81 74 52 50 14.7  1065829 64 59 4531 3.7 1065858 69 49 28 23 2.2 1065859 52 36 28 20 N.C. 1065901 89 69 7355 N.C. 1065914 108 111 82 74 N.C. 1065977 106 87 101 76 N.C. 1066009 8871 130 84 N.C. 1066046 84 59 43 36 5.5 1066089 46 71 58 41 N.C. 106621767 64 46 41 6.5 1066221 59 49 39 31 2.0 1066311 93 75 73 49 N.C. 106637794 73 51 35 7.9 1066396 100 75 82 54 N.C.

TABLE 49 Dose-dependent percent reduction of human UBE3A-ATS RNA invitro (42,000 cells/well) Compound UBE3A-ATS expression (% UTC) IC₅₀Number 740.7 nM 2,222 nM 6,666 nM 20,000 nM (μM) 617557 66 47 21 6 1.7750519 75 58 36 25 3.5 1065272 90 63 38 33 5.4 1065576 21 28 19 13 N.C.1065590 74 49 31 27 2.8 1065591 83 60 29 13 3.2 1065607 68 47 28 17 2.11065608 72 38 18 13 1.7 1065623 46 28 21 13 N.C. 1065669 89 75 57 28 7.61065685 64 34 19 20 1.2 1065795 100 86 56 38 10.8  1065810 62 46 23 221.6 1065812 77 67 36 24 4.1 1065826 108 93 54 43 12.1  1065937 86 61 6037 8.7 1065953 77 46 34 29 3.0 1065954 78 62 45 38 6.1 1066097 133 121119 125 N.C.

TABLE 50 Dose-dependent percent reduction of human UBE3A-ATS RNA invitro (42,000 cells/well) Compound UBE3A-ATS expression (% UTC) IC₅₀Number 740.7 nM 2,222 nM 6,666 nM 20,000 nM (μM) 617557 39 43 25 13 N.C.750519 44 36 34 17 N.C. 1065605 50 48 29 19 N.C. 1065619 95 73 47 5 4.61065621 5 63 44 21 4.6 1065635 48 29 19 4 N.C. 1065651 13 8 29 10 N.C.1065696 77 71 61 7 4.7 1065712 58 40 34 20 1.3 1065713 4 4 7 6 N.C.1065728 73 54 42 25 3.5 1065823 7 9 10 N/A N.C. 1065840 73 61 41 32 4.41065856 78 49 37 24 3.2 1065857 10 61 49 28 5.1 1065889 58 81 61 62 N.C.1065903 85 65 60 6 4.6 1065920 53 42 40 26 1.0 1066350 89 101 78 2111.3 

TABLE 51 Dose-dependent percent reduction of human UBE3A-ATS RNA invitro (42,000 cells/well) Compound UBE3A-ATS expression (% UTC) IC₅₀Number 740.7 nM 2,222 nM 6,666 nM 20,000 nM (μM) 617557 14 16 3 0 N.C.750519 31 27 22 12 N.C. 1065296 78 56 45 7 3.2 1065330 13 5 4 3 N.C.1065586 5 44 22 11 N.C. 1065600 79 55 36 4 2.9 1065616 84 75 65 5 5.41065708 9 8 6 N/A N.C. 1065709 6 4 4 4 N.C. 1065710 52 33 16 9 N.C.1065821 110 58 29 16 4.3 1065868 73 42 28 6 2.0 1065902 8 60 35 24 3.61065932 51 42 35 17 0.9 1065947 65 48 38 10 2.1 1066076 81 76 41 22 5.01066092 80 49 40 27 3.6 1066253 0 71 31 21 4.5 1066429 28 17 6 6 N.C.

TABLE 52 Dose-dependent percent reduction of human UBE3A-ATS RNA invitro (42,000 cells/well) Compound UBE3A-ATS expression (% UTC) IC₅₀Number 740.7 nM 2,222 nM 6,666 nM 20,000 nM (μM) 617557 37 17 29 12 N.C.750519 50 40 40 20 N.C. 1065295 78 73 74 49 N.C. 1065473 31 29 16 11N.C. 1065503 105 107 107 77 N.C. 1065561 66 44 21 13 1.7 1065593 59 4530 6 1.5 1065609 59 50 34 6 1.7 1065625 70 56 41 22 3.2 1065641 43 31 187 N.C. 1065671 55 49 30 8 1.4 1065678 75 78 54 33 8.0 1065765 64 46 2812 1.8 1065791 7 58 44 30 4.1 1065806 24 72 82 74 N.C. 1065813 12 11 117 N.C. 1065924 107 94 79 41 18.4  1066011 94 85 83 50 N.C. 1066220 24 8375 62 N.C.

TABLE 53 Dose-dependent percent reduction of human UBE3A-ATS RNA invitro (40,000 cells/well) Compound UBE3A-ATS expression (% UTC) IC₅₀Number 740.7 nM 2,222 nM 6,666 nM 20,000 nM (μM) 750519 83 53 21 14 2.81165521 55 38 32 27 0.9 1165524 42 33 26 14 N.C. 1165536 47 39 32 21N.C. 1165545 62 49 32 33 2.0 1165552 53 35 24 16 0.8 1165553 55 34 24 180.8 1165554 63 55 29 19 2.1 1165555 53 43 21 22 0.9 1165562 72 69 35 233.8 1165577 65 59 33 28 2.8 1165588 78 57 33 38 4.3 1165590 44 41 30 21N.C. 1165593 85 92 83 50 N.C. 1165611 47 39 25 21 N.C. 1165724 80 71 5754 N.C. 1165788 66 71 42 25 4.2 1165798 73 81 86 52 N.C. 1179842 78 3727 20 2.2

Example 5 Effect of Modified Oligonucleotides on Human UBE3A-ATS RNA InVitro, Multiple Doses

Modified oligonucleotides selected from the examples above were testedat various doses in human IPS derived ReproNeuro™ Neurons (Repro CELL).Cells were plated at a density of 20,000 cells per well, maintained permanufacturer instructions, and treated with modified oligonucleotides byfree uptake at various concentrations as specified in the tables below.After a treatment period of approximately 5 days, total RNA was isolatedfrom the cells and UBE3A-ATS RNA levels were measured by quantitativereal-time PCR. Human UBE3A-ATS primer probe set RTS4796, describedhereinabove, was used to measure RNA levels. UBE3A-ATS RNA levels werenormalized according to total RNA content, as measured by RIBOGREEN®.Reduction of UBE3A-ATS RNA is presented in the tables below as percentUBE3A-ATS RNA amount relative to untreated control (UTC) cells.

The half maximal inhibitory concentration (IC₅₀) of each modifiedoligonucleotide was calculated using a linear regression on a log/linearplot of the data in excel. In cases where IC₅₀ could not be calculated,IC₅₀s are marked as N.C. (Not Calculated).

TABLE 54 Dose-dependent percent reduction of human UBE3A-ATS RNA invitro Compound UBE3A-ATS expression (% UTC) IC₅₀ Number 740.7 nM 2,222nM 6,666 nM 20,000 nM (μM) 750519 73 54 19 11 2.3 1165523 75 60 43 334.7 1165533 60 50 18 33 1.5 1165538 64 47 34 26 2.1 1165550 65 62 47 324.5 1165563 69 51 41 29 3.1 1165586 62 50 35 32 2.3 1165596 88 72 69 4316.4  1165608 88 89 62 54 N.C. 1165616 89 88 80 74 N.C. 1165694 71 57 5550 15.0  1165737 71 55 53 46 9.1 1165827 72 59 65 42 13.6  1165855 60 6037 19 2.5 1165897 78 76 54 50 N.C. 1179808 101 100 85 75 N.C. 1179839 5841 26 23 1.2 1179841 68 45 38 26 2.4 1179843 52 57 36 21 1.7

Example 6 Effect of Modified Oligonucleotides on Human UBE3A-ATS RNA andUBE3A RNA In Vitro, Multiple Doses

Modified oligonucleotides selected from the examples above were testedat various doses in 10-week differentiated human neuronal cells derivedfrom Angelman Syndrome patient derived IPS cells (Protocols and cellsdescribed in Chamberlain SJ., et al., Induced pluripotent stem cellmodels of the genomic imprinting disorders Angelman and Prader-Willisyndromes. PNAS, 2010. 41: 17668-17673). At the end of the 10-weekdifferentiation period, cells were treated with modifiedoligonucleotides by free uptake at various concentrations as specifiedin the tables below. After a treatment period of approximately 6 days,total RNA was isolated from the cells. Both UBE3A-ATS RNA and UBE3A RNAlevels were measured by quantitative real-time PCR. Human UBE3A-ATSprimer probe set RTS4796 was used to measure UBE3A-ATS RNA levels asdescribed above. Human UBE3A primer probe set RTS35984 (forward sequenceCACCCTGATGTCACCGAATG, designated herein as SEQ ID NO: 8; reversesequence GCGTTCTATTAGATGCTTTGCAG, designated herein as SEQ ID NO: 9;probe sequence ACTGAGGTTCTCCTGATCTTTTACAAGCTG, designated herein as SEQID NO: 10) was used to measure UBE3A RNA levels. RNA levels wereadjusted according to total RNA content, as measured by RIBOGREEN®.Reduction of UBE3A-ATS RNA or induction of UBE3A RNA is presented in thetables below as percent UBE3A-ATS RNA amount or percent UBE3A RNArelative to untreated control (UTC) cells. Values marked with “N.D.”indicate that a value was not determined in this experiment.

Several modified oligonucleotides were found to reduce UBE3A-ATS RNAaccompanied by concurrent increase in UBE3A RNA in Angelman's patientIPS cell derived neurons.

TABLE 55 Reduction of UBE3A-ATS RNA and increase of UBE3A RNA inAngelman Syndrome IPS cell derived neurons UBE3A-ATS RNA UBE3A RNA Com-(% UTC) (RTS4796) (% UTC) (RTS35984) pound 741 2222 6667 741 2222 6667ID nM nM nM nM nM nM 617557 17 16 N.D. 142 151 N.D. 750140 7 N.D. N.D.226 N.D. N.D. 750139 35 10  3 104 196 194 750131 29 25 N.D. 49  53 N.D.750141 22 N.D. N.D. 89 N.D. N.D. 750242 42 N.D. N.D. 45 N.D. N.D. 75021049 14  6 122 101 166 750214 53 56 13 87 154  85 749907 16 13  7 133 298205 749931 35 17 11 152 271 260 749964 27 15  6 144 160 202 749921 25 12N.D. 73  98 N.D. 749933 42 10 13 116  92 210 749956 51 33 N.D. 94 103N.D. 749937 52 27  7 104 131 137 749796 73 67 N.D. 81  79 N.D. 749816 75N.D. 19 63 N.D.  27 749969 33 14 10 171 249 305 749991 32 15 15 193 165276

TABLE 56 Reduction of UBE3A-ATS RNA and increase of UBE3A RNA inAngelman Syndrome IPS cell derived neurons UBE3A-ATS RNA UBE3A RNA Com-(% UTC) (RTS4796) (% UTC) (RTS35984) pound 741 2222 6667 741 2222 6667ID nM nM nM nM nM nM 617557 54 51 39 144 365 252 750140 22 37 N.D. 345328 N.D. 750517 34 30 19 269 328 418 750549 60 72 72 337 225 309 75054264 70 22 98 113 234 617456 58 35 26 142 176 253 750519 33 26 18 374 440447 750360 93 113  59 151 221 204 750359 32 31 27 267 134 229 750386 3540 17 182 282 320 750366 77 45 37 87 122 185 750344 51 50 30 91 139 150750326 67 49 31 106 108 166 750350 68 66 102  111 132 138 750365 74 6337 122 160 183 750329 88 82 N.D. 154 148 N.D. 750028 81 N.D. 14 324 N.D.448 750030 87 30 22 221 307 373 750032 47 17 13 202 323 254

TABLE 57 Reduction of UBE3A-ATS RNA and increase of UBE3A RNA inAngelman Syndrome IPS cell derived neurons UBE3A-ATS RNA UBE3A RNA Com-(% UTC) (RTS4796) (% UTC) (RTS35984) pound 741 2222 6667 741 2222 6667ID nM nM nM nM nM nM 617557 28 54 N.D. 318 411 N.D. 750140 19 20 10 619542 132 750040 38 49 25 171 192 318 750051 21 21 13 106 544 416 75009240 32 12 175 507 118 749894 12 19 6 143 2085 605 749869 10 11 13 376 8021504  749882 34 31 19 502 907 926 749863 45 42 22 326 2584 927 749893 3410 7 215 428 1023  749885 22 12 10 105 233 614 749861 19 11 8 209 236466 750292 28 15 13 123 318 645 750270 18 14 10 267 578 584 750312 39 1912 87 289 420 750413 46 49 20 123 375 143 750416 49 N.D. N.D. 119 N.D.N.D. 750430 33 21 12 329 359 241 750439 40 31 19 188 197 183

TABLE 58 Reduction of UBE3A-ATS RNA and increase of UBE3A RNA inAngelman Syndrome IPS cell derived neurons UBE3A-ATS RNA UBE3A RNA Com-(% UTC) (RTS4796) (% UTC) (RTS35984) pound 741 2222 6667 741 2222 6667ID nM nM nM nM nM nM 617557 60 39 N.D. 266 161 N.D. 617557 65 23 8 229204 122 750140 18 29 N.D. 312 222 N.D. 617470 36 34 23 141 191 317617473 29 37 20 91 162 87 617459 38 18 8 80 155 191 617547 31 19 28 52145 226 617536 83 28 109 165 108 265 617593 67 142 107 100 73 83 75054498 107 51 221 258 307 750554 65 72 88 57 80 55 750540 46 26 29 202 344458 750567 62 49 31 86 108 227 749984 47 42 N.D. 68 99 277 750009 31 3922 65 92 217 749865 44 N.D. 12 201 N.D. 249 749860 36 22 9 285 265 263750006 32 17 66 241 329 513 582468 102 79 33 120 33 62 141923 150 169100 135 126 53

TABLE 59 Reduction of UBE3A-ATS RNA and increase of UBE3A RNA inAngelman Syndrome IPS cell derived neurons UBE3A-ATS RNA UBE3A RNA Com-(% UTC) (RTS4796) (% UTC) (RTS35984) pound 741 2222 6667 20000 741 22226667 20000 ID nM nM nM nM nM nM nM nM 749894 20 13 11 10 301 200 159 546749969 47 44 45 51 125 88 85 205 750032 16 17 12 17 215 190 182 236750140 26 33 13 22 252 429 160 222 1065690 18 7 11 9 313 295 203 2541065868 32 17 9 9 249 303 211 143 1065579 20 19 25 7 155 134 133 2311065858 22 24 25 17 190 239 229 249 1065859 55 57 36 29 117 141 199 1931065812 25 29 9 10 133 120 185 163 749860 35 21 14 11 129 250 196 2781065593 18 10 7 9 228 181 211 261 1065953 15 11 12 10 148 269 243 2211065856 15 18 11 9 292 286 103 234 1065937 21 26 12 10 93 205 225 2081065728 35 24 20 12 132 143 144 198 750139 38 28 13 14 176 229 248 315617557 55 23 30 33 166 206 193 226 750519 15 15 5 3 173 101 220 324

TABLE 60 Reduction of UBE3A-ATS RNA and increase of UBE3A RNA inAngelman Syndrome IPS cell derived neurons UBE3A-ATS RNA UBE3A RNA Com-(% UTC) (RTS4796) (% UTC) (RTS35984) pound 741 2222 6667 20000 741 22226667 20000 ID nM nM nM nM nM nM nM nM 1066092 138 160 98 55 247 253 254111 1065902 92 85 55 42 168 231 203 304 1065840 57 54 37 22 209 176 141307 1066253 77 37 52 36 182 167 184 236 1065785 93 35 41 54 155 198 225240 1065821 40 19 20 20 220 288 215 276 750006 78 33 33 56 132 119 168185 750028 36 17 16 19 173 235 189 216 617557 37 26 13 23 194 166 214241

TABLE 61 Reduction of UBE3A-ATS RNA and increase of UBE3A RNA inAngelman Syndrome IPS cell derived neurons UBE3A-ATS RNA UBE3A RNA Com-(% UTC) (RTS4796) (% UTC) (RTS35984) pound 741 2222 6667 20000 741 22226667 20000 ID nM nM nM nM nM nM nM nM 750519 41 26 11 8 138 158 148 1581065671 106 81 108 61 213 189 387 235 617557 69 52 37 35 129 132 210 1951065686 101 101 81 88 145 105 118 164 750359 121 70 79 48 108 119 134131 1065817 75 78 40 N.D. 119 174 131 N.D. 750386 100 65 58 50 106 155190 128 749894 58 40 19 20 176 187 159 244 1065591 68 51 52 34 138 132227 155 750140 178 165 133 129 129 117 98 142 750032 95 56 40 31 109 144126 172 1065599 73 45 42 33 135 131 188 210 1065690 66 48 35 29 182 175211 185 1065868 132 125 107 90 93 125 113  99 1065645 83 47 50 25 169144 287 158 1065858 78 46 22 18 175 192 162 227 1065856 76 66 49 35 138195 167 165 1065667 137 127 137 94 115 116 163 134 1066092 133 126 87 75100 113 104 106

Example 7 Design and Synthesis of Modified OligonucleotidesComplementary to a Human UBE3A-ATS Nucleic Acid

Modified oligonucleotides were synthesized as indicated in the tablesbelow.

The compounds in Table 62 are 4-10-6 MOE gapmers. The gapmers are 20nucleosides in length, wherein the central gap segment consists of ten2′-β-D-deoxynucleosides, the 5′ wing segment consists of four 2′-MOEnucleosides, and the 3′ wing segment consists of six 2′-MOE nucleosides.The sugar motif of the gapmers is (from 5′ to 3′): eeeeddddddddddeeeeee;wherein ‘0:1’ represents a 2′-β-D-deoxyriboxyl sugar moiety, and ‘e’represents a 2′-MOE sugar moiety. The gapmers have an internucleosidelinkage motif of (from 5′ to 3′): sooossssssssssoooss; wherein “s”represents a phosphorothioate internucleoside linkage and “o” representsa phosphodiester internucleoside linkage. All cytosine residues are5-methylcytosines.

TABLE 62 4-10-6 MOE gapmers with a mixed PO/PS internucleo-side linkages complementary to human UBE3A-ATS SEQ ID NO: 1 SEQ ID SEQCompound Start NO: 1 ID ID Site Stop Site SEQUENCE NO 1263473 460996461015 TTTTTCCATTTTTCTCTTAG 2745 1263474 460997 461016GTTTTTCCATTTTTCTCTTA 2746 1263475 460999 461018 GTGTTTTTCCATTTTTCTCT 5981263476 461000 461019 TGTGTTTTTCCATTTTTCTC 2747 1263477 461001 461020GTGTGTTTTTCCATTTTTCT 2748 1263478 461002 461021 TGTGTGTTTTTCCATTTTTC2749 1263479 465235 465254 TCATCTCGGGTATATAAATT 2750 1263480 465236465255 GTCATCTCGGGTATATAAAT 1132 1263481 465237 465256GGTCATCTCGGGTATATAAA 1207 1263482 465238 465257 TGGTCATCTCGGGTATATAA 3181263483 465239 465258 TTGGTCATCTCGGGTATATA 2473 1263484 465240 465259ATTGGTCATCTCGGGTATAT 1655 1263485 465241 465260 TATTGGTCATCTCGGGTATA2548 1263486 468985 469004 TCACCATTTTGACCTTCTTA 2751 1263487 468986469005 TTCACCATTTTGACCTTCTT 2752 1263488 468987 469006CTTCACCATTTTGACCTTCT 2753 1263489 468988 469007 GCTTCACCATTTTGACCTTC 3771263490 468989 469008 TGCTTCACCATTTTGACCTT 2754 1263491 468990 469009CTGCTTCACCATTTTGACCT 2755 1263492 468991 469010 ACTGCTTCACCATTTTGACC2756 1263494 464526 464545 TCAGTTAGGTTAGTGCACAG 2757 1263495 464527464546 CTCAGTTAGGTTAGTGCACA 2758 1263496 464528 464547GCTCAGTTAGGTTAGTGCAC 1504 1263497 464529 464548 TGCTCAGTTAGGTTAGTGCA2759 1263498 464530 464549 CTGCTCAGTTAGGTTAGTGC 2729 1263499 464531464550 TCTGCTCAGTTAGGTTAGTG 2760 1263500 479994 480013GAGCTATCTGTACAAAATGG 2761 1263501 479995 480014 TGAGCTATCTGTACAAAATG2743 1263502 479996 480015 GTGAGCTATCTGTACAAAAT 2744 1263503 479997480016 CGTGAGCTATCTGTACAAAA 1142 1263532 483970 483989GCATACCCAGGGTAGGATTC 765 1263534 483971 483990 TGCATACCCAGGGTAGGATT 14451263536 483972 483991 GTGCATACCCAGGGTAGGAT 766 1263539 483973 483992TGTGCATACCCAGGGTAGGA 2762 1263541 483974 483993 ATGTGCATACCCAGGGTAGG2595 1273009 457735 457754 GCCAGGTGTCTTATATCTAT 2852 1273010 474393474412 GGTCAACCAATTTGCTATTC 1809 1273011 474394 474413AGGTCAACCAATTTGCTATT 2853 1273012 474396 474415 TTAGGTCAACCAATTTGCTA2854 1273013 457736 457755 AGCCAGGTGTCTTATATCTA 2855 1273014 478535478554 AACGCAATGTATCAGGCAAC 2856 1273015 478536 478555AAACGCAATGTATCAGGCAA 2857 1273016 478730 478749 GATCACATTACCCATCCGTT2858 1273017 478731 478750 TGATCACATTACCCATCCGT 2859 1273018 478732478751 CTGATCACATTACCCATCCG 2860 1273019 474395 474414TAGGTCAACCAATTTGCTAT 2861 1273020 478733 478752 GCTGATCACATTACCCATCC1291 1273021 478734 478753 TGCTGATCACATTACCCATC 2862 1273022 478735478754 TTGCTGATCACATTACCCAT 2863

The compounds in Table 63 are 5-10-5 MOE gapmers. The gapmers are 20nucleosides in length, wherein the central gap segment consists of ten2′-β-D-deoxyribonucleosides and the 5′ and 3′ wing segments each consistof five 2′-MOE nucleosides. The sugar motif for the gapmers is (from 5′to 3′): eeeeeddddddddddeeeee; wherein ‘d’ represents a2′-β-D-deoxyribosyl sugar moiety, and ‘e’ represents a 2′-MOE sugarmoiety The gapmers have an internucleoside linkage motif of (from 5′ to3′): soooossssssssssooss, wherein “s” represents a phosphorothioateinternucleoside linkage and “o” represents a phosphodiesterinternucleoside linkage. All cytosine residues are 5-methylcytosines.

TABLE 63 5-10-5 MOE gapmers with a mixed PO/PS internucleo-side linkages complementary to human UBE3A-ATS SEQ ID NO: 1 SEQ ID SEQCompound Start NO: 1 ID ID Site Stop Site SEQUENCE NO 1263451 460996461015 TTTTTCCATTTTTCTCTTAG 2745 1263452 460997 461016GTTTTTCCATTTTTCTCTTA 2746 1263453 461000 461019 TGTGTTTTTCCATTTTTCTC2747 1263454 461001 461020 GTGTGTTTTTCCATTTTTCT 2748 1263455 461002461021 TGTGTGTTTTTCCATTTTTC 2749 1263456 465235 465254TCATCTCGGGTATATAAATT 2750 1263460 468985 469004 TCACCATTTTGACCTTCTTA2751 1263461 468986 469005 TTCACCATTTTGACCTTCTT 2752 1263462 468987469006 CTTCACCATTTTGACCTTCT 2753 1263463 468989 469008TGCTTCACCATTTTGACCTT 2754 1263464 468990 469009 CTGCTTCACCATTTTGACCT2755 1263465 468991 469010 ACTGCTTCACCATTTTGACC 2756 1263466 464526464545 TCAGTTAGGTTAGTGCACAG 2757 1263467 464527 464546CTCAGTTAGGTTAGTGCACA 2758 1263468 464529 464548 TGCTCAGTTAGGTTAGTGCA2759 1263469 464531 464550 TCTGCTCAGTTAGGTTAGTG 2760 1263470 479994480013 GAGCTATCTGTACAAAATGG 2761 1263471 479995 480014TGAGCTATCTGTACAAAATG 2743 1263472 479996 480015 GTGAGCTATCTGTACAAAAT2744 1263538 483973 483992 TGTGCATACCCAGGGTAGGA 2762 1272943 467048467067 TTTCATCAGTTAGTCAGGTT 2786 1272944 465605 465624CCTTTCTATTTCAGACCGAA 2787 1272945 465607 465626 TGCCTTTCTATTTCAGACCG2788 1272946 465608 465627 GTGCCTTTCTATTTCAGACC 2789 1272947 465609465628 AGTGCCTTTCTATTTCAGAC 2790 1272948 468919 468938AGTATAGATGCCTCTCCTCT 2791 1272949 468920 468939 AAGTATAGATGCCTCTCCTC2792 1272950 468921 468940 TAAGTATAGATGCCTCTCCT 2793 1272951 474796474815 ATTGACACCTCCAACTGTAA 2794 1272952 474798 474817GTATTGACACCTCCAACTGT 2795 1272953 474800 474819 TGGTATTGACACCTCCAACT2796 1272954 474801 474820 TTGGTATTGACACCTCCAAC 2797 1272955 474802474821 TTTGGTATTGACACCTCCAA 2798 1272956 476012 476031GTTTTCGCCCGTTACCTCAA 2799 1272957 476013 476032 AGTTTTCGCCCGTTACCTCA2800 1272958 476014 476033 CAGTTTTCGCCCGTTACCTC 2801 1272959 476017476036 CTCCAGTTTTCGCCCGTTAC 2802 1272960 467052 467071ACACTTTCATCAGTTAGTCA 2803 1272961 476018 476037 TCTCCAGTTTTCGCCCGTTA2804 1272962 478437 478456 GCTATAGGTGTCACATATTC 2805 1272963 478442478461 TTGTAGCTATAGGTGTCACA 2806 1272964 478443 478462TTTGTAGCTATAGGTGTCAC 2807 1272965 483106 483125 GCAATGGACTTAGTACACAA2808 1272966 483107 483126 GGCAATGGACTTAGTACACA 2809 1272967 483110483129 TTAGGCAATGGACTTAGTAC 2810 1272968 483111 483130CTTAGGCAATGGACTTAGTA 2811 1272969 485766 485785 CAGATTCCTAAATACGCACA2812 1272970 485767 485786 TCAGATTCCTAAATACGCAC 2813 1272971 485768485787 GTCAGATTCCTAAATACGCA 2814 1272972 485771 485790GTGGTCAGATTCCTAAATAC 2815 1272973 487601 487620 AGTGTCATATGTAGCAATTA2816 1272974 487603 487622 TTAGTGTCATATGTAGCAAT 2817 1272975 501337501356 TATGTAGCTCAGCTCAATGT 2818 1272976 501339 501358CTTATGTAGCTCAGCTCAAT 2819 1272977 501342 501361 CTGCTTATGTAGCTCAGCTC2820 1272978 468734 468753 AAAATCCATTTGTCCAGTCT 2821 1272979 505552505571 TTTGCTTTTCAGTCAGGTAC 2822 1272980 468735 468754TAAAATCCATTTGTCCAGTC 2823 1272981 468736 468755 CTAAAATCCATTTGTCCAGT2824 1272982 506110 506129 GCATTGGCTTCATATTTCTC 2825 1272983 506112506131 GAGCATTGGCTTCATATTTC 2826 1272984 508942 508961CATTATTCTCTAGTGCCTAT 2827 1272985 508943 508962 TCATTATTCTCTAGTGCCTA2828 1272986 508947 508966 GTCTTCATTATTCTCTAGTG 2829 1272987 508948508967 AGTCTTCATTATTCTCTAGT 2830 1272988 458439 458458AACTTCATCAATATTTCCCC 2831 1272989 458397 458416 TGTGGGCCTCTATTAAGATC2832 1272990 458399 458418 CATGTGGGCCTCTATTAAGA 2833 1272991 458401458420 TGCATGTGGGCCTCTATTAA 2834 1272992 458453 458472TTTATACTTTACCCAACTTC 2835 1272993 458454 458473 CTTTATACTTTACCCAACTT2836 1272994 458455 458474 GCTTTATACTTTACCCAACT 2837 1272995 458440458459 CAACTTCATCAATATTTCCC 2838 1272996 458457 458476TGGCTTTATACTTTACCCAA 2839 1272997 458458 458477 TTGGCTTTATACTTTACCCA2840 1272998 458459 458478 TTTGGCTTTATACTTTACCC 2841 1272999 458569458588 CTTTAGTATGTCGAGAACTC 2842 1273000 458441 458460CCAACTTCATCAATATTTCC 2843 1273001 461466 461485 ACTCATCATGATCTTGGTAA2844 1273002 463053 463072 TCACTGAGTTTTTGTAGTTC 2845 1273003 463191463210 TCTGGAATCTTGTAGAGGAT 2846 1273004 463192 463211TTCTGGAATCTTGTAGAGGA 2847 1273005 467047 467066 TTCATCAGTTAGTCAGGTTA2848 1273006 464395 464414 ATTCAGCACTTAGGTAGTTC 2849 1273007 464399464418 TCCCATTCAGCACTTAGGTA 2850 1273008 464400 464419TTCCCATTCAGCACTTAGGT 2851 1273061 468982 469001 CCATTTTGACCTTCTTAGCC2872 1273062 468983 469002 ACCATTTTGACCTTCTTAGC 2873 1273063 465232465251 TCTCGGGTATATAAATTAAT 2874 1273064 465233 465252ATCTCGGGTATATAAATTAA 2875 1273065 468992 469011 AACTGCTTCACCATTTTGAC2876 1273066 468993 469012 TAACTGCTTCACCATTTTGA 2877 1273067 468994469013 TTAACTGCTTCACCATTTTG 2878 1273068 464522 464541TTAGGTTAGTGCACAGATAA 2879 1273069 464523 464542 GTTAGGTTAGTGCACAGATA2880 1273070 464534 464553 GTCTCTGCTCAGTTAGGTTA 2881 1273071 479991480010 CTATCTGTACAAAATGGAAC 2882 1273072 479992 480011GCTATCTGTACAAAATGGAA 2883 1273073 479993 480012 AGCTATCTGTACAAAATGGA2884 1273084 457732 457751 AGGTGTCTTATATCTATGAT 2885 1273085 457742457761 GAAACCAGCCAGGTGTCTTA 2886 1273086 474391 474410TCAACCAATTTGCTATTCAT 2887 1273087 474392 474411 GTCAACCAATTTGCTATTCA2888 1273088 457733 457752 CAGGTGTCTTATATCTATGA 2889 1273089 474394474413 AGGTCAACCAATTTGCTATT 2853 1273090 474395 474414TAGGTCAACCAATTTGCTAT 2861 1273091 474396 474415 TTAGGTCAACCAATTTGCTA2854 1273092 474397 474416 TTTAGGTCAACCAATTTGCT 2890 1273093 474398474417 GTTTAGGTCAACCAATTTGC 2891 1273094 474399 474418GGTTTAGGTCAACCAATTTG 2892 1273095 478527 478546 GTATCAGGCAACAGAATCTC2893 1273096 478528 478547 TGTATCAGGCAACAGAATCT 2894 1273097 478529478548 ATGTATCAGGCAACAGAATC 2895 1273098 457734 457753CCAGGTGTCTTATATCTATG 2896 1273099 478535 478554 AACGCAATGTATCAGGCAAC2856 1273101 478537 478556 AAAACGCAATGTATCAGGCA 2897 1273102 478538478557 TAAAACGCAATGTATCAGGC 2898 1273103 457735 457754GCCAGGTGTCTTATATCTAT 2852 1273104 478727 478746 CACATTACCCATCCGTTCTT2899 1273105 478728 478747 TCACATTACCCATCCGTTCT 2900 1273106 478729478748 ATCACATTACCCATCCGTTC 2901 1273107 478730 478749GATCACATTACCCATCCGTT 2858 1273108 478731 478750 TGATCACATTACCCATCCGT2859 1273109 478732 478751 CTGATCACATTACCCATCCG 2860 1273110 478734478753 TGCTGATCACATTACCCATC 2862 1273111 478735 478754TTGCTGATCACATTACCCAT 2863 1273112 457736 457755 AGCCAGGTGTCTTATATCTA2855 1273113 478737 478756 TCTTGCTGATCACATTACCC 2902 1273114 478738478757 TTCTTGCTGATCACATTACC 2903 1273115 457737 457756CAGCCAGGTGTCTTATATCT 2904

The compounds in Table 64 are 6-10-4 MOE gapmers. The gapmers are 20nucleosides in length, wherein the central gap segment consists of ten2′-β-D-deoxynucleosides, the 5′ wing segment consists of six 2′-MOEnucleosides, and the 3′ wing segment consists of four 2′-MOEnucleosides. The sugar motif of the gapmers is (from 5′ to 3′):eeeeeeddddddddddeeee; wherein ‘d’ represents a 2′-β-D-deoxyriboxyl sugarmoiety, and ‘e’ represents a 2′-MOE sugar moiety. The gapmers have aninternucleoside linkage motif of (from 5′ to 3′): sooooossssssssssoss,wherein “s” represents a phosphorothioate internucleoside linkage and“o” represents a phosphodiester internucleoside linkage. All cytosineresidues are 5-methylcytosines.

TABLE 64 6-10-4 MOE gapmers with a mixed PO/PS internucleo-side linkages complementary to human UBE3A-ATS SEQ ID NO: 1 SEQ ID SEQCompound Start NO: 1 ID ID Site Stop Site SEQUENCE NO 1263457 479995480014 TGAGCTATCTGTACAAAATG 2743 1263458 479996 480015GTGAGCTATCTGTACAAAAT 2744 1263459 479997 480016 CGTGAGCTATCTGTACAAAA1142 1263504 460996 461015 TTTTTCCATTTTTCTCTTAG 2745 1263505 460997461016 GTTTTTCCATTTTTCTCTTA 2746 1263506 460999 461018GTGTTTTTCCATTTTTCTCT 598 1263507 461000 461019 TGTGTTTTTCCATTTTTCTC 27471263508 461001 461020 GTGTGTTTTTCCATTTTTCT 2748 1263509 461002 461021TGTGTGTTTTTCCATTTTTC 2749 1263510 465235 465254 TCATCTCGGGTATATAAATT2750 1263511 465236 465255 GTCATCTCGGGTATATAAAT 1132 1263512 465237465256 GGTCATCTCGGGTATATAAA 1207 1263513 465238 465257TGGTCATCTCGGGTATATAA 318 1263514 465239 465258 TTGGTCATCTCGGGTATATA 24731263515 465240 465259 ATTGGTCATCTCGGGTATAT 1655 1263516 465241 465260TATTGGTCATCTCGGGTATA 2548 1263517 468985 469004 TCACCATTTTGACCTTCTTA2751 1263518 468986 469005 TTCACCATTTTGACCTTCTT 2752 1263519 468987469006 CTTCACCATTTTGACCTTCT 2753 1263520 468988 469007GCTTCACCATTTTGACCTTC 377 1263521 468989 469008 TGCTTCACCATTTTGACCTT 27541263522 468990 469009 CTGCTTCACCATTTTGACCT 2755 1263523 468991 469010ACTGCTTCACCATTTTGACC 2756 1263524 464525 464544 CAGTTAGGTTAGTGCACAGA2728 1263525 464526 464545 TCAGTTAGGTTAGTGCACAG 2757 1263526 464527464546 CTCAGTTAGGTTAGTGCACA 2758 1263527 464528 464547GCTCAGTTAGGTTAGTGCAC 1504 1263528 464529 464548 TGCTCAGTTAGGTTAGTGCA2759 1263529 464530 464549 CTGCTCAGTTAGGTTAGTGC 2729 1263530 464531464550 TCTGCTCAGTTAGGTTAGTG 2760 1263531 479994 480013GAGCTATCTGTACAAAATGG 2761 1263533 483970 483989 GCATACCCAGGGTAGGATTC 7651263535 483971 483990 TGCATACCCAGGGTAGGATT 1445 1263537 483972 483991GTGCATACCCAGGGTAGGAT 766 1263540 483973 483992 TGTGCATACCCAGGGTAGGA 27621263542 483974 483993 ATGTGCATACCCAGGGTAGG 2595 1273023 457735 457754GCCAGGTGTCTTATATCTAT 2852 1273024 457736 457755 AGCCAGGTGTCTTATATCTA2855 1273025 474393 474412 GGTCAACCAATTTGCTATTC 1809 1273026 474394474413 AGGTCAACCAATTTGCTATT 2853 1273027 474395 474414TAGGTCAACCAATTTGCTAT 2861 1273028 474396 474415 TTAGGTCAACCAATTTGCTA2854 1273029 478535 478554 AACGCAATGTATCAGGCAAC 2856 1273030 478536478555 AAACGCAATGTATCAGGCAA 2857 1273031 478730 478749GATCACATTACCCATCCGTT 2858 1273032 478731 478750 TGATCACATTACCCATCCGT2859 1273033 478732 478751 CTGATCACATTACCCATCCG 2860 1273034 478733478752 GCTGATCACATTACCCATCC 1291 1273035 478734 478753TGCTGATCACATTACCCATC 2862 1273036 478735 478754 TTGCTGATCACATTACCCAT2863

The compounds in Table 65 are 4-8-6 MOE gapmers. The gapmers are 18nucleosides in length, wherein the central gap segment consists of eight2′-β-D-deoxynucleosides, the 5′ segment consists of four 2′-MOEnucleosides, and the 3′ wing segment consists of six 2′-MOE nucleosides.The sugar motif of the gapmers is (from 5′ to 3′): eeeeddddddddeeeeee;wherein ‘d’ represents a 2′-β-D-deoxyriboxyl sugar moiety, and ‘e’represents a 2′-MOE sugar moiety. The gapmers have an internucleosidelinkage motif of (from 5′ to 3′): soosssssssssoooss; wherein “s”represents a phosphorothioate internucleoside linkage and “o” representsa phosphodiester internucleoside linkage. All cytosine residues are5-methylcytosines.

TABLE 65 4-8-6 MOE gapmers with a mixed PO/PS internucleo-side linkages complementary to human UBE3A-ATS SEQ ID NO: 1 SEQ ID SEQCompound Start NO: 1 ID ID Site Stop Site SEQUENCE NO 1263403 465237465254 TCATCTCGGGTATATAAA 2768 1263404 465238 465255 GTCATCTCGGGTATATAA2769 1263405 465239 465256 GGTCATCTCGGGTATATA 2770 1263406 465240 465257TGGTCATCTCGGGTATAT 2771 1263407 465241 465258 TTGGTCATCTCGGGTATA 27721263408 468987 469004 TCACCATTTTGACCTTCT 2773 1263409 468988 469005TTCACCATTTTGACCTTC 2774 1263410 468989 469006 CTTCACCATTTTGACCTT 27751263411 468991 469008 TGCTTCACCATTTTGACC 2776 1263412 464527 464544CAGTTAGGTTAGTGCACA 2777 1263413 464528 464545 TCAGTTAGGTTAGTGCAC 27781263414 464529 464546 CTCAGTTAGGTTAGTGCA 2779 1263415 464530 464547GCTCAGTTAGGTTAGTGC 2780 1263416 464531 464548 TGCTCAGTTAGGTTAGTG 27811263417 479996 480013 GAGCTATCTGTACAAAAT 2782 1263418 479997 480014TGAGCTATCTGTACAAAA 2783 1263419 479998 480015 GTGAGCTATCTGTACAAA 27841263420 479999 480016 CGTGAGCTATCTGTACAA 2785 1263543 483971 483988CATACCCAGGGTAGGATT 2763 1263546 483972 483989 GCATACCCAGGGTAGGAT 27641263549 483973 483990 TGCATACCCAGGGTAGGA 2765 1263552 483974 483991GTGCATACCCAGGGTAGG 2766 1263557 483975 483992 TGTGCATACCCAGGGTAG 27671273037 457737 457754 GCCAGGTGTCTTATATCT 2864 1273038 478535 478552CGCAATGTATCAGGCAAC 2865 1273039 478536 478553 ACGCAATGTATCAGGCAA 28661273040 478732 478749 GATCACATTACCCATCCG 2867 1273041 478733 478750TGATCACATTACCCATCC 2868 1273042 478735 478752 GCTGATCACATTACCCAT 28691273043 457738 457755 AGCCAGGTGTCTTATATC 2870 1273060 474393 474410TCAACCAATTTGCTATTC 2871

The compounds in Table 66 are 5-8-5 MOE gapmers. The gapmers are 18nucleosides in length, wherein the central gap segment consists of eight2′-β-D-deoxynucleosides and the 5′ and 3′ wing segments each consists offive 2′-MOE nucleosides. The sugar motif of the gapmers is (from 5′ to3′): eeeeeddddddddeeeee; wherein ‘d’ represents a 2′-β-D-deoxyriboxylsugar moiety, and ‘e’ represents a 2′-MOE sugar moiety. The gapmers havean internucleoside linkage motif of (from 5′ to 3′): sooosssssssssooss;wherein “s” represents a phosphorothioate internucleoside linkage and“o” represents a phosphodiester internucleoside linkage. All cytosineresidues are 5-methylcytosines.

TABLE 66 5-8-5 MOE gapmers with a mixed PO/PS internucleo-side linkages complementary to human UBE3A-ATS SEQ ID NO: 1 SEQ ID SEQCompound Start NO: 1 ID ID Site Stop Site SEQUENCE NO 1263421 465237465254 TCATCTCGGGTATATAAA 2768 1263422 465238 465255 GTCATCTCGGGTATATAA2769 1263423 465239 465256 GGTCATCTCGGGTATATA 2770 1263424 465240 465257TGGTCATCTCGGGTATAT 2771 1263425 465241 465258 TTGGTCATCTCGGGTATA 27721263426 468987 469004 TCACCATTTTGACCTTCT 2773 1263427 464527 464544CAGTTAGGTTAGTGCACA 2777 1263428 464528 464545 TCAGTTAGGTTAGTGCAC 27781263429 464529 464546 CTCAGTTAGGTTAGTGCA 2779 1263430 464530 464547GCTCAGTTAGGTTAGTGC 2780 1263431 464531 464548 TGCTCAGTTAGGTTAGTG 27811263432 479996 480013 GAGCTATCTGTACAAAAT 2782 1263433 479997 480014TGAGCTATCTGTACAAAA 2783 1263434 479998 480015 GTGAGCTATCTGTACAAA 27841263435 479999 480016 CGTGAGCTATCTGTACAA 2785 1263544 483971 483988CATACCCAGGGTAGGATT 2763 1263547 483972 483989 GCATACCCAGGGTAGGAT 27641263550 483973 483990 TGCATACCCAGGGTAGGA 2765 1263553 483974 483991GTGCATACCCAGGGTAGG 2766 1263554 483975 483992 TGTGCATACCCAGGGTAG 27671273052 457737 457754 GCCAGGTGTCTTATATCT 2864 1273053 457738 457755AGCCAGGTGTCTTATATC 2870 1273054 474393 474410 TCAACCAATTTGCTATTC 28711273055 478535 478552 CGCAATGTATCAGGCAAC 2865 1273056 478536 478553ACGCAATGTATCAGGCAA 2866 1273057 478732 478749 GATCACATTACCCATCCG 28671273058 478733 478750 TGATCACATTACCCATCC 2868 1273059 478735 478752GCTGATCACATTACCCAT 2869

The compounds in Table 67 are 6-8-4 MOE gapmers. The gapmers are 18nucleosides in length, wherein the central gap segment consists of eight2′-β-D-deoxynucleosides, the 5′ wing segment consists of six 2′-MOEnucleosides, and the 3′ wing segment consists of four 2′-MOEnucleosides. The sugar motif of the gapmers is (from 5′ to 3′):eeeeeeddddddddeeee; wherein ‘d’ represents a 2′-β-D-deoxyriboxyl sugarmoiety, and ‘e’ represents a 2′-MOE sugar moiety. The gapmers have aninternucleoside linkage motif of (from 5′ to 3′): soooosssssssssoss;wherein “s” represents a phosphorothioate internucleoside linkage and“o” represents a phosphodiester internucleoside linkage. All cytosineresidues are 5-methylcytosines.

TABLE 67 6-8-4 MOE gapmers with a mixed PO/PS internucleo-side linkages complementary to human UBE3A-ATS SEQ ID NO: 1 SEQ ID SEQCompound Start NO: 1 ID ID Site Stop Site SEQUENCE NO 1263436 465237465254 TCATCTCGGGTATATAAA 2768 1263437 465238 465255 GTCATCTCGGGTATATAA2769 1263438 465239 465256 GGTCATCTCGGGTATATA 2770 1263439 465240 465257TGGTCATCTCGGGTATAT 2771 1263440 465241 465258 TTGGTCATCTCGGGTATA 27721263441 468987 469004 TCACCATTTTGACCTTCT 2773 1263442 464527 464544CAGTTAGGTTAGTGCACA 2777 1263443 464528 464545 TCAGTTAGGTTAGTGCAC 27781263444 464529 464546 CTCAGTTAGGTTAGTGCA 2779 1263445 464530 464547GCTCAGTTAGGTTAGTGC 2780 1263446 464531 464548 TGCTCAGTTAGGTTAGTG 27811263447 479996 480013 GAGCTATCTGTACAAAAT 2782 1263448 479997 480014TGAGCTATCTGTACAAAA 2783 1263449 479998 480015 GTGAGCTATCTGTACAAA 27841263450 479999 480016 CGTGAGCTATCTGTACAA 2785 1263545 483971 483988CATACCCAGGGTAGGATT 2763 1263548 483972 483989 GCATACCCAGGGTAGGAT 27641263551 483973 483990 TGCATACCCAGGGTAGGA 2765 1263555 483974 483991GTGCATACCCAGGGTAGG 2766 1263556 483975 483992 TGTGCATACCCAGGGTAG 27671273044 457737 457754 GCCAGGTGTCTTATATCT 2864 1273045 457738 457755AGCCAGGTGTCTTATATC 2870 1273046 474393 474410 TCAACCAATTTGCTATTC 28711273047 478535 478552 CGCAATGTATCAGGCAAC 2865 1273048 478536 478553ACGCAATGTATCAGGCAA 2866 1273049 478732 478749 GATCACATTACCCATCCG 28671273050 478733 478750 TGATCACATTACCCATCC 2868 1273051 478735 478752GCTGATCACATTACCCAT 2869

Example 8 Effect of Modified Oligonucleotides on Human UBE3A-ATS RNA InVitro, Multiple Doses

Modified oligonucleotides selected from the examples above were testedat various doses in human IPS derived iCell GABANeurons (CellularDynamics), as described in Example 4. Reduction of UBE3A-ATS RNA ispresented in the tables below as percent UBE3A-ATS RNA amount relativeto untreated control (UTC) cells. Where possible, the half maximalinhibitory concentration (IC₅₀) of each modified oligonucleotide wascalculated using a linear regression on a log/linear plot of the data inexcel. In some cases, an IC₅₀ could not be reliably calculated and thedata point is marked as “N.C.”. Values marked with “N.D.” indicate thata value was not determined in this experiment.

TABLE 68 Dose-dependent percent reduction of human UBE3A-ATS RNA invitro (40,000 cells/well) UBE3A-ATS expression (% UTC) Compound 250740.7 2,222 6,666 20,000 IC₅₀ Number nM nM nM nM nM (μM) 750519 69 53 23N.D. N.D. 0.7 617557 66 54 34 25 N.D. 0.9 749860 62 48 39 20 13 0.71065593 44 32 21 11 9 N.C. 1263517 55 47 35 17 18 0.4 1263519 52 39 2517 16 0.2 1263533 51 34 29 28 23 0.1 1263540 39 29 29 29 24 N.C. 127299451 39 28 14 10 0.2 1272996 62 45 38 20 13 0.6 1272997 73 53 39 24 18 1.11272998 67 53 30 19 13 0.8 1273030 68 54 33 30 22 1.0 1273033 51 50 3132 20 0.4 1273049 57 41 32 24 21 0.3

TABLE 69 Dose-dependent percent reduction of human UBE3A-ATS RNA invitro (40,000 cells/well) UBE3A-ATS expression (% UTC) Compound 250740.7 2,222 6,666 20,000 IC₅₀ Number nM nM nM nM nM (μM) 750519 79 66 2915 N.D. 1.1 1263426 64 48 34 29 25 0.7 1273042 92 72 58 43 40 5.51273051 70 47 34 29 22 0.9 1273055 71 58 47 40 27 1.9 1273057 73 71 5037 39 3.4 1273058 80 69 58 48 34 4.8 1273087 55 34 26 14 20 0.2 127310763 59 36 26 26 1.0 1273113 75 68 58 47 28 3.6

TABLE 70 Dose-dependent percent reduction of human UBE3A-ATS RNA invitro (40,000 cells/well) UBE3A-ATS expression (% UTC) Compound 250740.7 2,222 6,666 20,000 IC₅₀ Number nM nM nM nM nM (μM) 750519 56 38 2210 25 0.2 749860 68 47 37 19 16 0.8 1065645 66 45 31 19 12 0.6 126346170 66 55 32 27 2.3 1263486 74 48 44 25 21 1.2

TABLE 71 Dose-dependent percent reduction of human UBE3A-ATS RNA invitro (40,000 cells/well) UBE3A-ATS expression (% UTC) Compound 250740.7 2,222 6,666 20,000 IC₅₀ Number nM nM nM nM nM (μM) 750519 63 39 199 4 0.4 1263517 56 40 27 17 11 0.3 1263518 72 65 42 28 20 1.6 1263532 5744 38 33 64 0.5 1263533 41 56 37 ND 26 NC

TABLE 72 Dose-dependent percent reduction of human UBE3A-ATS RNA invitro (40,000 cells/well) UBE3A-ATS expression (% UTC) Compound 250740.7 2,222 6,666 20,000 IC₅₀ Number nM nM nM nM nM (μM) 617557 59 56 3723 23 0.8 1263537 70 86 82 77 59 N.C. 1272944 51 39 23 13 8 0.2 127303355 40 32 21 15 0.3 1273039 68 58 37 28 20 1.1

TABLE 73 Dose-dependent percent reduction of human UBE3A-ATS RNA invitro (40,000 cells/well) UBE3A-ATS expression (% UTC) Compound 250740.7 2,222 6,666 20,000 IC₅₀ Number nM nM nM nM nM (μM) 617557 70 57 38N.D. N.D. 1.0 1273050 81 75 76 62 41 16.5  1273055 59 48 40 26 18 0.61273062 54 37 28 18 13 0.2 1273090 76 64 71 56 55 N.C. 1273091 75 79 5644 32 4.3

Example 9 Effect of Modified Oligonucleotides on Human UBE3A-ATS RNA andUBE3A RNA In Vitro, Multiple Doses

Modified oligonucleotides selected from the examples above were testedat various doses in 10-week differentiated human neuronal cells derivedfrom Angelman Syndrome patient derived IPS cells as described in Example6 above. Reduction of UBE3A-ATS RNA or induction of UBE3A RNA ispresented in the tables below as percent UBE3A-ATS RNA amount or percentUBE3A RNA relative to untreated control (UTC) cells. Values marked with“N.D.” indicate that a value was not determined in this experiment.

Several modified oligonucleotides were found to reduce UBE3A-ATS RNAaccompanied by concurrent increase in UBE3A RNA in Angelman's patientIPS cell derived neurons.

TABLE 74 Reduction of UBE3A-ATS RNA and increase of UBE3A RNA inAngelman Syndrome IPS cell derived neurons UBE3A-ATS RNA UBE3A RNA Com-(% UTC) (RTS4796) (% UTC) (RTS35984) pound 250 741 2222 6667 20000 250741 2222 6667 20000 ID nM nM nM nM nM nM nM nM nM nM 750519 51 20 11 7 5297 224 235 250 205 749860 47 21 18 15 8 148 119 245 307 324 1065645 4115 13 11 13 213 185 298 315 353 1263461 49 42 34 25 16 141 185 248 235221 1263486 64 47 33 29 16 136 174 215 323 264

TABLE 75 Reduction of UBE3A-ATS RNA and increase of UBE3A RNA inAngelman Syndrome IPS cell derived neurons UBE3A-ATS RNA UBE3A RNA Com-(% UTC) (RTS4796) (% UTC) (RTS35984) pound 250 741 2222 6667 20000 250741 2222 6667 20000 ID nM nM nM nM nM nM nM nM nM nM 750519 46 22 13 7 7259 274 520 482 272 1263517 37 29 16 12 12 230 261 462 521 283 126351866 39 29 21 18 187 183 269 496 277 1263532 39 26 21 17 24 192 162 393390 357 1263533 34 25 24 18 29 296 367 389 371 548

TABLE 76 Reduction of UBE3A-ATS RNA and increase of UBE3A RNA inAngelman Syndrome IPS cell derived neurons UBE3A-ATS RNA UBE3A RNA Com-(% UTC) (RTS4796) (% UTC) (RTS35984) pound 250 741 2222 6667 20000 250741 2222 6667 20000 ID nM nM nM nM nM nM nM nM nM nM 617557 68 44 28 2420 120 210 319 453 396 1263537 29 26 21 26 16 139 203 414 440 4801272944 35 25 13 11 7 148 235 469 702 677 1273033 38 21 21 16 18 119 244487 422 514 1273039 64 41 29 20 17 183 237 395 376 418

TABLE 77 Reduction of UBE3A-ATS RNA and increase of UBE3A RNA inAngelman Syndrome IPS cell derived neurons UBE3A-ATS RNA UBE3A RNA Com-(% UTC) (RTS4796) (% UTC) (RTS35984) pound 250 741 2222 6667 20000 250741 2222 6667 20000 ID nM nM nM nM nM nM nM nM nM nM 617557 63 50 33 2827 126 230 347 335 368 1273050 64 48 59 41 28 165 152 240 457 4831273055 44 31 38 22 17 541 664 1014 1094 887 1273062 34 54 51 47 27 253296 421 705 997 1273090 70 54 30 39 23 155 165 259 487 692 1273091 91 8654 37 31 96 121 135 156 284

Example 10 Tolerability of Modified Oligonucleotides Complementary toHuman UBE3A-ATS in Wild-Type Mice, 3 Hour Study

Modified oligonucleotides described above were tested in wild-typefemale C57/B16 mice to assess the tolerability of the oligonucleotides.Wild-type female C57/B16 mice each received a single ICV dose of 700 μgof modified oligonucleotide listed in the table below. Each treatmentgroup consisted of 4 mice. A group of 4 mice received PBS as a negativecontrol for each experiment (identified in separate tables below). At 3hours post-injection, mice were evaluated according to seven differentcriteria. The criteria are (1) the mouse was bright, alert, andresponsive; (2) the mouse was standing or hunched without stimuli; (3)the mouse showed any movement without stimuli; (4) the mousedemonstrated forward movement after it was lifted; (5) the mousedemonstrated any movement after it was lifted; (6) the mouse respondedto tail pinching; (7) regular breathing. For each of the 7 criteria, amouse was given a subscore of 0 if it met the criteria and 1 if it didnot (the functional observational battery score or FOB). After all 7criteria were evaluated, the scores were summed for each mouse andaveraged within each treatment group. The results are presented in thetables below.

TABLE 78 Tolerability scores in mice at 700 μg dose Compound Number 3 hrFOB PBS 0.0 749860 0.0 749865 4.0 749984 4.8 750006 3.5 750009 3.8750540 3.5 750544 2.8 750567 5.0

TABLE 79 Tolerability scores in mice at 700 μg dose Compound Number 3 hrFOB PBS 0 1065272 5.0 1065295 2.0 1065296 5.5 1065324 2.8 1065330 6.81065369 6.8 1065438 5.8 1065465 5.0 1065513 5.8 1065576 7.0 1065578 3.21065579 3.8 1065582 4.2 1065586 3.0 1065590 6.0 1065591 4.0 1065593 1.01065595 4.8 1065597 4.5 1065599 6.0 1065600 4.2 1065605 3.8 1065607 5.21065608 3.0

TABLE 80 Tolerability scores in mice at 700 μg dose Compound Number 3 hrFOB PBS 0.0 1065609 0.0 1065613 0.0 1065616 5.5 1065619 5.0 1065621 2.01065623 3.5 1065624 3.5 1065631 1.0 1065635 1.0 1065641 1.5 1065642 4.01065644 6.8 1065645 3.0 1065646 2.0 1065651 4.0 1065654 7.0 1065667 4.01065669 1.0 1065671 4.0 1065672 6.8 1065674 4.0 1065676 2.0 1065678 5.01065680 1.0

TABLE 81 Tolerability scores in mice at 700 μg dose Compound Number 3 hrFOB PBS 0 1065685 5.0 1065686 4.8 1065690 3.8 1065696 6.2 1065708 5.51065709 4.2 1065710 2.8 1065712 6.8 1065713 5.0 1065719 4.0 1065728 1.01065735 3.5 1065750 6.2 1065754 3.2 1065765 4.2 1065766 5.2 1065768 5.51065785 3.8 1065795 1.0 1065799 1.0 1065810 2.2 1065812 3.8 1065813 5.01065817 5.0

TABLE 82 Tolerability scores in mice at 700 μg dose Compound Number 3 hrFOB PBS 0.0 1065821 6.5 1065823 4.0 1065826 3.0 1065829 4.8 1065840 4.51065856 6.2 1065857 6.0 1065858 4.0 1065859 0.0 1065863 4.8 1065868 3.21065889 1.0 1065894 3.8 1065899 4.5 1065901 4.0 1065902 4.0 1065903 5.51065914 5.0 1065920 2.0 1065932 4.5 1065937 1.0 1065947 1.2 1065953 6.51065954 5.5

TABLE 83 Tolerability scores in mice at 700 μg dose Compound Number 3 hrFOB PBS 0.0 1065955 5.0 1065977 6.2 1066009 2.0 1066037 4.2 1066046 1.01066072 4.0 1066076 5.0 1066089 1.0 1066092 1.0 1066097 1.2 1066119 11.01066217 6.0 1066221 3.8 1066249 4.5 1066253 6.0 1066311 2.0 1066350 4.01066375 1.0 1066377 6.2 1066378 5.2 1066396 6.0 1066420 4.0 1066423 2.81066429 5.8

TABLE 84 Tolerability scores in mice at 700 μg dose Compound Number 3 hrFOB PBS 0.0 749931 0.0 749933 4.0 749937 0.5 749944 3.8 749956 1.8749964 2.0 749969 5.2 749991 2.2 750028 4.2 750030 3.2 750032 2.2 7500404.2 750051 2.5 750092 0.8 750100 2.5 750131 0.5 750139 3.0 750140 1.2750196 4.0 750210 3.5 750214 3.25 750228 0.0 750270 1.0 750292 0.0750312 4.8 750325 1.0 750326 0.0 750329 5.5 750344 6.2 750350 2.8 7503590.0 750360 3.2 750365 5.2 750366 0.0 750386 0.0 750413 0.0 750416 7.0750418 1.8 750430 6.2 750431 5.0 750439 5.5 750452 2.5 750517 3.0 7505191.0 750542 3.5 750549 0.0

TABLE 85 Tolerability scores in mice at 700 μg dose Compound Number 3 hrFOB PBS 0.0 617456 4.8 617459 7.0 617470 6.5 617473 6.8 617536 6.5617547 6.8 617557 5.2 617593 6.5 749794 5.0 749796 4.5 749816 5.8 7498613.0 749863 5.5 749869 3.8 749882 6.8 749885 0.5 749893 1.2 749894 0.0749907 5.0 749921 6.0

TABLE 86 Tolerability scores in mice at 700 μg dose Compound Number 3 hrFOB PBS 0.0 1065473 3.2 1065561 6.0 1065592 4.0 1065625 7.0 1065791 1.01065924 1.0 1066003 4.0

TABLE 87 Tolerability scores in mice at 700 μg dose Compound ID 3 hr FOBPBS 0.0 1263471 6.2 1263472 3.2 1263473 1.0 1263474 0.0 1263475 0.01263476 1.0 1263477 0.0 1263478 1.0 1263479 4.0 1263480 4.0 1263481 5.51263482 4.0 1263483 4.0 1263484 4.0 1263485 4.0 1263486 2.0 1263487 1.01263488 1.0 1263489 0.0 1263490 0.0 1263491 0.0 1263492 1.0 1263494 4.01263495 4.0 1263496 4.0 1263497 4.0 1263498 4.0 1263499 4.0 1263500 4.71263501 5.5 1263502 4.0 1263503 4.0 1263504 1.0 1263505 0.0 1263506 0.01263507 0.0 1263508 0.0 1263509 0.0 1263510 2.0

TABLE 88 Tolerability scores in mice at 700 μg dose Compound ID 3 hr FOBPBS 0.0 1165878 4.0 1263511 4.0 1263512 6.0 1263513 6.0 1263514 6.51263515 4.0 1263516 4.0 1263517 1.0 1263518 0.0 1263519 1.0 1263520 1.01263521 0.0 1263522 0.0 1263523 1.0 1263524 5.2 1263525 4.8 1263526 4.01263527 4.0 1263528 4.0 1263529 3.0 1263530 4.0 1263531 5.2 1263532 1.01263533 1.0 1263534 1.0 1263535 4.0 1263536 2.0 1263537 1.0 1263538 3.01263539 1.0 1263540 0.0

TABLE 89 Tolerability scores in mice at 700 μg dose Compound ID 3 hr FOBPBS 0.0 1263541 1.8 1263542 3.5 1263543 1.0 1263544 1.0 1263545 2.01263546 0.0 1263547 1.0 1263548 1.0 1263549 0.0 1263550 1.0 1263551 2.01263552 1.0 1263553 5.5 1263554 1.0 1263555 4.0 1263556 1.0 1263557 1.0

TABLE 90 Tolerability scores in mice at 700 μg dose Compound ID 3 hr FOBPBS 0.0 1263403 5.3 1263404 4.3 1263405 6.3 1263406 4.7 1263407 2.31263408 0.3 1263409 0.0 1263410 0.0 1263411 0.0 1263412 2.0 1263413 2.31263414 5.3 1263415 4.0 1263416 7.0 1263417 3.3 1263418 3.0 1263419 1.71263420 1.0 1263421 3.0 1263422 4.3 1263423 6.0 1263424 6.3 1263425 5.0

TABLE 91 Tolerability scores in mice at 700 μg dose Compound ID 3 hr FOBPBS 0.0 749952 6.0 749953 3.3 1165583 5.7 1165584 3.3 1263426 2.01263427 4.0 1263428 4.3 1263429 1.3 1263430 2.3 1263431 6.3 1263432 6.01263433 4.7 1263434 1.0 1263435 1.3 1263436 2.3 1263437 4.7 1263438 6.01263439 4.7 1263440 6.7 1263441 0.3 1263442 3.7 1263443 3.0 1263444 6.31263445 5.0 1263446 5.7 1263447 4.7 1263448 5.0 1263449 4.7 1263450 2.01263451 1.3 1263452 0 1263453 0

TABLE 92 Tolerability scores in mice at 700 μg dose Compound ID 3 hr FOBPBS 0 1065786 2.0 1165526 0.7 1165577 3.7 1263454 0.0 1263455 0.01263456 3.3 1263457 5.3 1263458 1.3 1263459 1.0 1263460 1.0 1263461 1.01263462 1.0 1263463 1.0 1263464 1.3 1263465 1.7 1263466 5.0 1263467 6.01263468 4.3 1263469 7.0 1263470 6.7 1272943 2.0 1272944 2.3 1272945 2.71272946 0.0 1272947 0.0 1272948 3.3 1272949 4.0 1272950 3.7 1272951 0.71272952 2.0 1272953 0.3 1272954 1.0

TABLE 93 Tolerability scores in mice at 700 μg dose Compound ID 3 hr FOBPBS 0.0 1272955 3.7 1272956 5.3 1272957 4.0 1272958 5.0 1272959 3.31272960 1.0 1272961 4.7 1272962 2.0 1272963 4.0 1272964 2.3 1272965 1.01272966 2.3 1272967 1.0 1272968 1.0 1272969 0.0 1272970 0.0 1272971 0.01272972 0.0 1272973 4.0 1272974 1.0 1272975 1.0 1272976 2.0 1272977 1.01272978 2.3 1272979 1.0

TABLE 94 Tolerability scores in mice at 700 μg dose Compound ID 3 hr FOBPBS 0.0 1272980 3.3 1272981 3.3 1272982 1.0 1272983 1.0 1272984 4.01272985 1.0 1272986 4.7 1272987 1.0 1272988 1.0 1272989 1.0 1272990 4.01272991 4.0 1272992 0.0 1272993 0.0 1272994 1.0 1272995 1.0 1272996 0.71272997 1.0 1272998 0.0 1272999 5.0 1273000 0.0 1273001 3.0 1273002 3.71273003 2.7 1273004 3.0 1273005 4.0 1273006 3.3 1273007 3.0 1273008 3.31273009 1.0 1273010 4.7 1273011 1.0

TABLE 95 Tolerability scores in mice at 700 μg dose Compound ID 3 hr FOBPBS 0.0 699781 0.0 1065707 2.0 1065711 4.0 1065921 5.7 1165585 3.71165621 1.0 1273012 0.0 1273013 4.0 1273014 2.0 1273015 2.0 1273016 0.01273017 1.0 1273018 0.0 1273019 0.0 1273020 0.0 1273021 0.0 1273022 0.01273023 0.0 1273024 0.0 1273025 5.0 1273026 5.3 1273027 5.0 1273028 0.01273029 4.7 1273030 3.0 1273031 0.0 1273032 0.0 1273033 0.0

TABLE 96 Tolerability scores in mice at 700 μg dose Compound ID 3 hr FOBPBS 0.0 1273034 0.0 1273035 1.0 1273036 1.0 1273037 1.0 1273038 1.01273039 1.0 1273040 0.0 1273041 0.0 1273042 0.0 1273043 6.0 1273044 0.01273045 0.0 1273046 3.0 1273047 3.0 1273048 0.0 1273049 0.0 1273050 0.01273051 0.0 1273052 0.0 1273053 6.0 1273054 3.0 1273055 2.0 1273056 2.31273057 2.0 1273058 1.0 1273059 1.0 1273060 0.7 1273061 1.0 1273062 0.01273063 0.0 1273064 0.0 1273065 0.0 1273066 0.0 1273067 1.0 1273068 6.01273069 6.0 1273070 1.0 1273071 0.0 1273072 0.0 1273073 4.0 1273084 0.01273085 3.0 1273086 2.0 1273087 1.0 1273088 0.0 1273089 5.3 1273090 0.01273091 0.0 1273092 0.0 1273093 1.0 1273094 6.0 1273095 4.3 1273096 4.71273097 5.0 1273098 1.0 1273099 1.0 1273101 0.7 1273102 1.0 1273103 0.01273104 0.0 1273105 0.0 1273106 0.0 1273107 0.0 1273108 0.0 1273109 1.01273110 0.0 1273111 0.0 1273112 2.3 1273113 0.0 1273114 0.0 1273115 2.3

TABLE 97 Tolerability scores in mice at 700 μg dose Compound ID 3 hr FOBPBS 0.0 1165523 2.0 1165533 2.3 1165538 3.0 1165550 1.3 1165563 1.31165586 2.3 1165596 2.3 1165608 4.0 1165616 3.0 1165694 3.3 1165737 3.71165827 3.3 1165855 3.0 1165897 0.3 1179808 2.3 1179839 6.7 1179841 2.01179843 2.0 1273100 7.0

TABLE 98 Tolerability scores in mice at 700 μg dose Compound Number 3 hrFOB PBS 0.0 1165521 3.3 1165524 4.7 1165536 4.0 1165545 6.0 1165552 5.71165553 6.0 1165554 7.0 1165555 6.0 1165562 5.7 1165588 4.7 1165590 5.71165611 6.0

Example 11 Tolerability of Modified Oligonucleotides Complementary toHuman UBE3A-ATS in Rats, 3 Hour Study

Modified oligonucleotides described above were tested in rats to assessthe tolerability of the oligonucleotides. Sprague Dawley rats eachreceived a single intrathecal (IT) dose of 3 mg of oligonucleotidelisted in the table below. Each treatment group consisted of 4 rats. Agroup of four rats received PBS as a negative control. At 3 hourspost-injection, movement in 7 different parts of the body were evaluatedfor each rat. The 7 body parts are (1) the rat's tail; (2) the rat'sposterior posture; (3) the rat's hind limbs; (4) the rat's hind paws;(5) the rat's forepaws; (6) the rat's anterior posture; (7) the rat'shead. For each of the 7 different body parts, each rat was given asub-score of 0 if the body part was moving or 1 if the body part wasparalyzed (the functional observational battery score or FOB). Aftereach of the 7 body parts were evaluated, the sub-scores were summed foreach rat and then averaged for each group. For example, if a rat's tail,head, and all other evaluated body parts were moving 3 hours after the 3mg IT dose, it would get a summed score of 0. If another rat was notmoving its tail 3 hours after the 3 mg IT dose but all other evaluatedbody parts were moving, it would receive a score of 1. Results arepresented as the average score for each treatment group.

TABLE 99 Tolerability scores in rats at 3 mg dose Compound No. FOB 3 hrPBS 0.0 749860 0.0 749861 4.0 749869 4.8 749885 0.8 749893 0.5 7499310.5 750006 4.5

TABLE 100 Tolerability scores in rats at 3 mg dose Compound No. FOB 3 hrPBS 0.0 750030 4.0 750051 4.0 750092 2.5 750100 4.0 750139 5.3 7501403.5 750270 3.0 750292 0.0 750325 1.8 750386 0.8

TABLE 101 Tolerability scores in rats at 3 mg dose Compound No. FOB 3 hrPBS 0.0 1065578 4.0 1065586 3.0 1065609 2.0 1065613 3.8 1065635 1.81065641 3.3 1065645 3.0 1065646 2.5

TABLE 102 Tolerability scores in rats at 3 mg dose Compound No. FOB 3 hrPBS 0.0 750413 0.3 1065669 2.8 1065680 4.0 1065710 5.5 1065754 5.31065785 6.0 1065795 3.3 1065799 2.0 1065812 4.0 1065826 2.0

TABLE 103 Tolerability scores in rats at 3 mg dose Compound No. FOB 3 hrPBS 0.0 1065920 3.5 1065937 3.8 1066092 3.3 1066221 4.0 1066350 2.3

TABLE 104 Tolerability scores in rats at 3 mg dose Compound No. FOB 3 hrPBS 0.3 1263408 2.0 1263409 2.5 1263410 2.5 1263411 1.3 1263412 3.01263419 1.8 1263420 1.8 1263429 2.5 1263434 2.0

TABLE 105 Tolerability scores in rats at 3 mg dose Compound No. FOB 3 hrPBS 0.3 1263435 3.0 1263441 2.3* 1263450 2.0 1263451 4.0 1263452 2.01263453 1.8 1263454 1.5 1263455 3.3 1263458 0.3 *One of the four rats inthis group died during surgery, and so is excluded from this value.

TABLE 106 Tolerability scores in rats at 3 mg dose Compound No. FOB 3 hrPBS 0.5 1263476 0.3 1263478 1.0 1263488 3.0 1263489 2.3 1263507 2.01263517 1.3 1263518 1.8 1263519 1.0 1263521 2.0 1263533 1.3

TABLE 107 Tolerability scores in rats at 3 mg dose Compound No. FOB 3 hrPBS 0.0 1165563 3.0 1179841 3.0 1179843 3.3 1263407 3.8 1263413 4.01263430 4.3 1263436 2.8 1263540 2.5 1272944 2.0 1272994 1.0 1272996 1.0

TABLE 108 Tolerability scores in rats at 3 mg dose Compound No. FOB 3 hrPBS 0.0 1272998 0.3 1273034 0.0 1273035 0.8 1273036 0.0 1273049 0.31273051 0.0. 1273055 2.0 1273056 3.7 1273057 1.0 1273058 0.8 1273062 2.51273065 2.3 1273087 2.0 1273099 2.5 1273102 4.5 1273106 2.0 1273107 2.01273108 2.0 1273113 1.0 1273115 3.3 1165523 5.0 1165550 3.0

TABLE 109 Tolerability scores in rats at 3 mg dose Compound No. FOB 3 hrPBS 0.0 1263426 2.3 1263462 2.8 1272974 3.8 1272997 0.8 1273020 0.51273033 1.8 1273038 3.0 1273039 3.3 1273042 2.0 1273048 3.0 1273104 3.01273105 3.0 1273109 3.8 1273110 1.0 1273111 2.7 1065593 1.5 1263547 4.01165586 0.0 1263537 1.5

TABLE 110 Tolerability scores in rats at 3 mg dose Compound No. FOB 3 hrPBS 0.0 749953 4.5 1065711 3.5 1263417 6.3 1263418 3.3 1263421 5.81263443 5.0 1263456 5.0 1263529 2.5 1263538 4.7 1273030 0.0 1273047 4.01273085 4.0

TABLE 111 Tolerability scores in rats at 3 mg dose Compound No. FOB 3 hrPBS 0.0 749991 1.3 1263460 3.0 1263461 2.0 1263486 3.0 1263532 1.51263551 4.0 1273050 1.0 1273090 1.0 1273091 2.0 1273101 3.5 1263522 1.7

Example 12 Tolerability of Modified Oligonucleotides Complementary toHuman UBE3A-ATS in Wild-Type Mice, Two Week Study

Modified oligonucleotides described above were tested in wild-typefemale C57/B16 mice to assess the tolerability of the oligonucleotides.Wild-type female C57/B16 mice each received a single ICV dose of 700 μgof modified oligonucleotide listed in the table below. Each treatmentgroup consisted of 4 mice. A group of 4 mice received PBS as a negativecontrol for each experiment (identified in separate tables below). At 2weeks post-injection, mice were evaluated according to seven differentcriteria. The criteria are (1) the mouse was bright, alert, andresponsive; (2) the mouse was standing or hunched without stimuli; (3)the mouse showed any movement without stimuli; (4) the mousedemonstrated forward movement after it was lifted; (5) the mousedemonstrated any movement after it was lifted; (6) the mouse respondedto tail pinching; (7) regular breathing. For each of the 7 criteria, amouse was given a subscore of 0 if it met the criteria and 1 if it didnot (the functional observational battery score or FOB). After all 7criteria were evaluated, the scores were summed for each mouse andaveraged within each treatment group. The results are presented in thetable below.

TABLE 112 Tolerability scores in mice at 700 μg dose Compound Number2-week FOB PBS 0.0 1065645 0.0 1263517 0.0 1263518 0.0 1263533 0.01273039 0.0 1273062 0.0

Example 13 Activity of Modified Oligonucleotides Complementary to HumanUBE3A-ATS in Transgenic Mice

Modified oligonucleotides described above were tested in a UBE3A-ATS BACtransgenic mouse model. The transgenic mouse model was developed at theUniversity of Michigan Bacterial Artificial Chromosome RecombineeringCore and contains region 25163935-25348867 from human chromosome 15(GRCh38/hg38 assembly) (from BAC clone RP11-664B13). UBE3A-ATSexpression is driven by the ENO2 promoter and terminated with the BGHpoly(A) signal. The gene fragment was introduced into fertilized eggsfrom C57BL/6 mice by pronuclear injection to produce line RP11-748 thatis used in the experiments described below.

Treatment

The UBE3A-ATS transgenic mice were divided into groups of 2-4 mice each.Each mouse received a single ICV bolus of 350 μg of modifiedoligonucleotide. A group of 4 mice received PBS as a negative control.

RNA Analysis

After two weeks, mice were sacrificed and RNA was extracted fromcortical brain tissue, hippocampal brain tissue, and spinal cord forreal-time PCR analysis of measurement of RNA expression of UBE3A-ATSusing primer probe set RTS4796 (described herein above) and/or primerprobe set RTS40595 (forward sequence

TCCTTCCCTACCTTAGTCTTGA, designated herein as SEQ ID NO: 14; reversesequence CCCTCTTGAACCAGGAAACA, designated herein as SEQ ID NO: 15; probesequence AGATGGCAGCCCACATTTCTACTGT, designated herein as SEQ ID NO: 16).Primer probe set RTS4796 is less reliable when the oligonucleotide bindsat a site far from the primer probe binding site. Results are presentedas percent change of RNA, relative to PBS control, normalized to mouseGAPDH. Mouse GAPDH was amplified using primer probe set RTS108 (forwardsequence GGCAAATTCAACGGCACAGT, designated herein as SEQ ID NO: 11;reverse sequence GGGTCTCGCTCCTGGAAGAT, designated herein as SEQ ID NO:12; probe sequence AAGGCCGAGAATGGGAAGCTTGTCATC, designated herein as SEQID NO: 13). In some cases, RTPCR value is not defined for a certainsample, and is labeled N.D. (Not Defined).

As shown in the table below, treatment with modified oligonucleotidesresulted in reduction of UBE3A-ATS RNA in comparison to the PBS control.

TABLE 113 Reduction of human UBE3A-ATS RNA in transgenic mice UBE3A-ATSRNA UBE3A-ATS RNA Com- (% control) RTS4796 (% control) RTS40595 poundSPINAL HIPPO- COR- SPINAL HIPPO- COR- ID CORD CAMPUS TEX CORD CAMPUS TEXPBS 100 100 100 100 100 100 749860 14 N.D. 26 12 24 23 749861 27 N.D. 5126 48 49 749863 39 N.D. 57 36 45 54 749865 61 N.D. 92 66 97 85 749869 3137 51 29 38 47 749882 31 28 35 30 30 32 749885 59 65 85 61 68 83 74989328 35 44 27 35 40 749894 5 7 9 3 6 7 749907 53 48 70 51 47 63 749931 3524 33 15 22 28 749969 17 14 14 11 13 10 749991 27 26 38 22 24 31 75000624 21 24 19 19 18 750028 26 22 34 17 19 26 750030 40 30 44 32 26 34750032 17 9 12 10 6 6 750040 55 42 65 50 44 59 750051 35 27 35 27 26 29750092 55 43 63 47 40 53 750139 24 22 30 17 20 23 750140 22 16 22 15 1315 750214 48 46 60 45 41 55 750292 27 35 40 21 31 34 750312 59 48 47 5343 42 750359 30 23 25 25 19 20 750360 44 40 54 39 37 47 750365 58 69 8856 65 82 750386 34 24 27 26 19 19 750413 45 37 38 36 34 30 750430 48 4241 41 42 33 750439 53 46 52 34 44 46 1065296 34 34 39 31 34 34 106557835 42 49 34 42 42 1065579 19 18 19 17 18 14 1065586 45 55 74 43 55 681065590 43 53 67 42 51 63 1065591 21 24 29 19 21 25 1065595 25 34 49 1330 41 1065599 19 22 27 18 22 24 1065600 31 35 42 32 35 39 1065605 44 6467 44 62 63 1065607 28 36 39 29 33 34 1065608 18 42 53 18 38 49 106562316 28 34 15 29 30 1065635 32 39 41 30 38 36 1065641 32 55 50 30 49 441065642 43 58 65 42 52 59 1065645 18 30 24 15 24 18 1065646 39 60 50 3954 45 1065651 29 47 38 28 43 34 1065667 23 30 26 19 24 22 1065671 21 2930 18 25 26 1065685 71 76 82 40 68 71 1065690 12 16 20 9 12 15 106570828 38 33 24 31 27 1065710 29 34 32 27 29 26 1065713 31 34 40 29 29 341065765 25 27 33 21 22 28 1065813 38 49 67 34 41 62 1065823 37 33 36 3024 28 1065858 23 25 21 15 14 13 1065859 29 20 22 19 11 13 1065863 52 4539 39 34 29 1065868 22 19 19 14 13 12 1065920 47 60 56 45 51 44 106593228 33 35 23 25 26 1065947 34 35 40 28 25 30 1066221 44 74 76 43 69 691066420 55 74 71 52 71 61 1066423 43 42 39 37 35 32 1066429 75 74 83 7065 72

TABLE 114 Reduction of human UBE3A-ATS RNA in transgenic mice UBE3A-ATSRNA UBE3A-ATS RNA Com- (% control) RTS4796 (% control) RTS40595 poundSPINAL HIPPO- COR- SPINAL HIPPO- COR- ID CORD CAMPUS TEX CORD CAMPUS TEXPBS 100 100 100 100 100 100 1065272 40 29 39 37 31 39 1065582 45 28 5243 31 51 1065616 38 25 45 36 25 42 1065619 33 23 54 31 27 55 1065621 4233 57 43 41 54 1065624 20 40 62 20 42 61 1065631 24 32 71 24 34 651065669 38 33 89 34 30 72 1065674 37 36 111 35 34 101 1065678 33 36 10532 35 96 1065680 43 61 134 40 59 121 1065686 21 25 63 19 22 55 106569644 36 117 40 32 108 1065719 63 36 122 56 32 109 1065728 20 11 53 14 9 421065754 47 25 112 41 24 105 1065766 29 41 110 24 41 98 1065768 40 41 9932 38 84 1065799 44 43 104 38 40 99 1065812 19 15 35 12 12 26 1065817 2923 60 22 20 52 1065821 23 19 42 16 17 34 1065829 36 19 80 27 18 581065840 27 15 61 21 14 45 1065856 22 19 44 14 13 30 1065857 35 28 58 2621 38 1065899 37 30 77 30 24 58 1065902 21 17 55 17 14 41 1065937 22 1291 17 11 65 1065953 19 13 58 13 10 41 1065955 34 19 69 26 16 53 106604658 35 122 50 32 106 1066076 46 27 76 41 25 N.D. 1066092 27 15 56 21 1347 1066217 37 26 68 32 26 62 1066253 27 15 50 22 14 39 1066377 50 30 7940 28 73

TABLE 115 Reduction of human UBE3A-ATS RNA in transgenic mice CompoundUBE3A-ATS RNA (% control) RTS4796 ID SPINAL CORD HIPPOCAMPUS CORTEX PBS100  100  100  1263473 43 71 66 1263474  8 15 10 1263475  6 10  81263476  6 12 14 1263477  4 10 11 1263478 13 18 17 1263486 27 27 321263487 31 43 43 1263488 23 24 14 1263489 16 17 16 1263490 24 28 231263491 22 25 21 1263492 29 50 58 1263504  9 11 12 1263505  6 11  71263506  5 10  6 1263507  5 14  6 1263508  7 15  9 1263509  6 17  71263510 37 70 72 1263517 13 22 17 1263518 19 36 30 1263519 20 25 151263520 15 19 11 1263521 20 44 20 1263522 22 29 35 1263523 21 30 321263532 29 49 40 1263533 18 24 17 1263534 27 52 37 1263536 30 45 361263537 29 43 34 1263539 51 59 88 1263540  22*  21*  29* 1263541 52 6680 1263543 49 362  84 1263544 53 100  104  1263545 36 54 57 1263546 4545 88 1263547 26 28 30 1263548 36 45 47 1263549 66 87 84 1263550 47 7576 1263551 30 45 39 1263552 66 65 93 1263554 72 75 88 1263556 60 59 831263557 86 91 109  1263408 45 70 46 1263409 29 44 33 1263410 29 64 421263411 42 72 58 1263419 47 126  65 1263420 73 130  72 1263429 27 33 361263434 44 50 42 1263435 47 42 61 1263441 22 17 29 1263451 21 16 281263452 14  8 15 1263453 13  9  9 *Only 1 animal in group

TABLE 116 Reduction of human UBE3A-ATS RNA in transgenic mice CompoundUBE3A-ATS RNA (% control) ID SPINAL CORD HIPPOCAMPUS CORTEX PBS 100 100100 1263454 6 8 13 1263455 5 19 35 1263458 39 62 88 1263459 28 45 521263460 11 28 34 1263461 24 30 35 1263462 25 24 25 1263463 36 40 661263464 25 22 38 1263465 32 35 39 1065786 121 75 32 1165526 116 78 461272943 41 73 32 1272946 37 69 33 1272947 55 94 54 1272951 70 89 751272952 125 85 109 1272953 86 78 51 1272954 110 89 70 1272955 137 136 701272960 59 77 40 1272962 45 52 26 1272964 74 58 62 1272965 43 39 251272966 52 68 67 1272967 65 69 62 1272968 76 62 66 1272969 83 60 1471272970 143 57 60 1272971 81 58 48 1272972 74 69 38 1272974 40 29 181272975 124 86 96 1272976 93 62 50 1272977 58 59 47 1272978 39 46 401272979 61 69 46 1272982 60 58 42 1272983 84 83 70 1272985 72 56 381272987 181 37 28 1272988 66 34 31 1272989 87 60 68 1272992 77 45 421272993 57 36 60 1272994 16 12 10 1272995 182 35 93 1272996 26 9 181272997 24 12 27 1272998 26 14 17 1273000 104 45 56 1273003 93 28 601273009 39 8 19 1273011 145 78 80 1273012 110 36 71 1273014 46 19 371273015 79 35 68 1273016 58 34 37 1273017 46 39 56 1273018 41 24 601273019 98 39 80 1273020 44 10 19 1273021 59 27 47 1273022 73 23 451273023 54 23 31 1273024 29 21 25 1273028 46 50 28 1273031 45 17 281273032 40 21 35 1273033 36 12 26 699781 66 25 37 1065707 78 44 701165621 107 41 71

TABLE 117 Reduction of human UBE3A-ATS RNA in transgenic mice CompoundUBE3A-ATS RNA (% control) ID SPINAL CORD HIPPOCAMPUS PBS 100 100 127303425 23 1273035 16 20 1273036 24 26 1273061 33 22 1273062 28 10 1273063 3229 1273064 38 25 1273065 27 16 1273066 34 36 1273067 44 42 1273070 25 231273071 57 71 1273072 38 48 1273037 12 25 1273038 16 29 1273039 15 291273040 39 33 1273041 48 29 1273042 51 17 1273044 45 27 1273045 48 241273048 30 18 1273049 19 15 1273050 30 31 1273051 17 21 1273052 14 151273055 14 20 1273056 16 22 1273057 16 18 1273058 20 22 1273059 16 171273060 36 35 1273084 41 35 1273087 12 14 1273088 33 28 1273090 24 411273091 26 31 1273092 37 38 1273093 28 23 1273098 17 23 1273099 14 101273101 29 32 1273102 20 18 1273103 12 5 1273104 57 16 1273105 34 91273106 30 11 1273107 24 8 1273108 31 13 1273109 30 13 1273110 37 81273111 46 12 1273113 25 10 1273114 46 26 1273115 15 7 1165523 38 171165533 31 19 1165538 64 25 1165550 47 11 1165563 30 12 1165586 35 191165596 69 49 1165616 109 25 1165855 49 16 1165897 39 28 1179808 83 361179841 22 9 1179843 22 11

Example 14 Activity of Modified Oligonucleotides Complementary to HumanUBE3A-ATS in Transgenic Mice, Multiple Doses

Modified oligonucleotides described above were tested in the UBE3A-ATSBAC transgenic mouse model RP11-748 line.

Treatment

The UBE3A-ATS transgenic mice were divided into groups of 3 mice each.Each mouse received a single ICV bolus of 10, 30, 100, 300, or 700 μg ofmodified oligonucleotide. and sacrificed two weeks later. A group of 8mice received PBS as a negative control.

RNA Analysis

After two weeks, mice were sacrificed, and RNA was extracted fromcortical brain tissue, hippocampal brain tissue, and spinal cords forreal-time PCR analysis of measurement of RNA expression of UBE3A-ATSusing primer probe set RTS40595 (forward sequenceTCCTTCCCTACCTTAGTCTTGA, designated herein as SEQ ID NO: 14; reversesequence CCCTCTTGAACCAGGAAACA, designated herein as SEQ ID NO: 15; probesequence AGATGGCAGCCCACATTTCTACTGT, designated herein as SEQ ID NO: 16).Results are presented as percent change of RNA, relative to PBS control,normalized to mouse GAPDH (measured by primer-probe set RTS108).

As shown in the table below, treatment with modified oligonucleotidesresulted in dose-dependent reduction of UBE3A-ATS RNA in comparison tothe PBS control.

TABLE 118 Dose-dependent percent reduction of human UBE3A-ATS RNA intransgenic mice Cortex Hippocampus Spinal Cord Compound Dose UBE3A-ATSRNA ED50 UBE3A-ATS RNA ED50 UBE3A-ATS RNA ED50 ID (μg) (% control) (μg)(% control) (μg) (% control) (μg) PBS 0 100 — 100 — 100 — 749860 1098.16531 138 81.99485 109 59.65406 17 30 84.95562 91.16468 45.18495 10067.32992 52.07824 25.79271 300 26.23195 33.3197 10.36557 700 11.9792116.72183 9.517061 1065645 10 90.14288 74 88.23692 71 37.14751 6 3072.43123 66.81222 16.11173 100 49.12943 51.83015 10.87515 300 16.3423525.55912 7.843655 1263461 10 88.27412 94 86.23292 126 60.06681 14 3081.19946 84.36883 33.7366 100 55.94376 68.69458 21.85816 300 16.2556823.08811 28.43356 700 11.55421 20.98394 18.26012 1263486 10 84.88829 9188.16814 110 80.05438 49 30 73.65319 88.23227 67.24883 100 53.5154946.18175 35.76668 300 29.17494 39.41106 25.80888 700 13.16333 21.5823614.67831 1263517 10 61.99356 27 78.62965 38 28.43223 5 30 57.3578264.77742 4.093424 100 28.22543 31.27599 3.855931 300 13.48909 11.499652.253222 700 6.218008 9.114471 0.845911 1263518 10 83.49507 47 74.920736 7.813722 N.C. 30 63.00462 58.90944 6.925703 100 36.83505 35.793519.984906 300 10.60636 16.33499 22.15675 1263532 10 87.76102 437 104.2908508 85.92361 126 30 93.21178 107.4279 86.97925 100 82.08274 79.8054356.10128 300 63.11973 71.02516 37.9068 1263533 10 85.64712 62 91.0865793 79.33594 35 30 74.82061 92.99996 56.07265 100 37.83251 50.4818232.95345 300 17.85169 15.11169 16.13775 700 13.22447 16.26845 14.777171263537 10 99.27557 167 101.6452 149 58.69295 32 30 61.11154 67.1288957.56289 100 70.65222 60.23925 35.5374 300 35.35647 38.67746 31.30235700 37.55784 42.24916 26.47357 1272944 10 85.26636 171 98.97738 24186.06921 66 30 83.3624 91.08809 60.22092 100 64.40395 69.68625 45.39798300 47.00232 54.90247 34.02307 700 18.19851 26.34956 26.27892 1273033 1092.81903 159 104.9703 202 71.92708 27 30 86.45458 92.23621 47.44761 10071.94214 74.17289 34.27954 300 28.94805 34.12991 17.19729 700 19.1499135.15452 16.60517 1273039 10 84.62214 63 93.89685 77 52.24285 33 3070.19074 70.45983 45.93645 100 40.06878 45.44749 30.60229 300 22.1840728.77613 27.68067 700 16.46246 23.35814 20.2156 1273050 10 60.65157 3476.1426 178 108.4293 45 30 46.72804 76.65907 59.53868 100 42.1323363.21802 30.24442 300 35.42049 42.84989 16.76795 700 27.88596 44.4296916.65603 1273055 10 54.09476 104 101.4753 139 95.64317 46 30 75.6099380.40454 46.396 100 62.55598 61.10855 43.64926 300 37.44794 36.8380826.42752 700 18.68964 24.28962 17.02671 1273062 10 83.90397 65 91.2581565 96.79799 85 30 71.94265 68.4521 78.51707 100 41.74674 43.1021945.07505 300 23.62598 26.78065 25.9929 700 8.048482 17.65158 20.99031273090 10 51.37967 25 83.05922 143 94.48769 120 30 53.46583 70.4691779.90936 100 41.09529 69.46586 53.85753 300 25.47022 49.33954 51.068041273091 10 48.66948 28 86.34518 195 126.1334 240 30 49.31716 78.33165118.9585 100 48.19272 67.14459 75.20145 300 31.19941 48.97407 42.91308700 28.63277 34.46767 43.51426

Example 15 Tolerability of Modified Oligonucleotides Complementary toHuman UBE3A-ATS in Wild-Type Mice, 3 Hour and 2 Week Study

Modified oligonucleotides closely matched to LNA/DNA oligonucleotidesdescribed in WO2017/081223 were tested in female wild-type C57/B16 miceto assess the tolerability of the oligonucleotides. See, CertainComparator Compouonds, hereinabove. Wild-type female C57/B16 mice eachreceived a single ICV dose of 700 μg of modified oligonucleotide listedin the table below. Each treatment group consisted of 4 mice. A group of4 mice received PBS as a negative control for the experiment. At 3 hourspost-injection and 2 weeks post-injection, mice were evaluated accordingto seven different criteria. The criteria are (1) the mouse was bright,alert, and responsive; (2) the mouse was standing or hunched withoutstimuli; (3) the mouse showed any movement without stimuli; (4) themouse demonstrated forward movement after it was lifted; (5) the mousedemonstrated any movement after it was lifted; (6) the mouse respondedto tail pinching; (7) regular breathing. For each of the 7 criteria, amouse was given a subscore of 0 if it met the criteria and 1 if it didnot (the functional observational battery score or FOB). After all 7criteria were evaluated, the scores were summed for each mouse andaveraged within each treatment group. The results are presented in thetable below.

TABLE 119 Tolerability scores in mice at 700 μg dose SEQ ID SEQ NO: 1ID NO: SEQ Compound 3 hr 2 week Start 1 Stop ID ID FOB FOB Site SiteChemistry Notation (5′ to 3′) NO PBS 0.0 0.0 1219022 7.0 n.d. 468275468294A_(ls)A_(ls)A_(ds)T_(ds)T_(ls)A_(ds)T_(ls)T_(ds)T_(ds)A_(ds)T_(ds)A_(ds)^(m)C_(ds)A_(ds) ^(m)C_(ds) ^(m)C_(ds)A_(ls)T_(ls) ^(m)C_(ls)A_(l) 29051219023 1.0 3.5 462549 462566 T_(ls)T_(ls)T_(ls)A_(ds)T_(ds)^(m)C_(ls)A_(ds)A_(ds)T_(ds)A_(ds)T_(ds)^(m)C_(ds)T_(dsds)T^(m)C_(ds)T_(ls) ^(m)C_(ls)A₁ 2906 1219024 5.3 6.5471876 471894 G_(ls) ^(m)C_(ds)A_(ls) ^(m)C_(ds)A_(ls)T_(ds)T_(ds)^(m)C_(ds)T_(ds)T_(ds)T_(ds) ^(m)C_(ds)T_(ds)A_(ds)T_(ds)A_(ds)^(m)C_(ds) ^(m)C_(ls)T₁ 2907 1219025 4.0 6.0 461987 462003T_(ls)T_(ls)A_(ds)T_(ls)A_(ls)Gds^(m)C_(ds) ^(m)C_(ds)A_(ds)T_(ds)T_(ds)^(m)C_(ds)T_(ds)A_(ds)T_(ds) ^(m)C_(ls)T₁ 2908 1219026 1.0 6.0 487371487387 ^(m)C_(ls)T_(ds) ^(m)C_(ls)A_(ls)A_(ls)A_(ds)GdsA_(ds)T_(ds)^(m)C_(ds)A_(ds)T_(ds)T_(ds) ^(m)C_(ls)T_(ls) ^(m)C_(ls)A₁ 2909 12190271.0 0.0 487430 487449 T_(ls)T_(ds)A_(ls) ^(m)C_(ds)A_(ls)^(m)C_(ds)T_(ls)T_(ds)A_(ds)A_(ds)T_(ds)T_(ds)A_(ds)T_(ds)A_(ds)^(m)C_(ds)T_(ls)T_(ds) ^(m)C_(ls) ^(m)C₁ 2910 1219028 6.0 6.0 496570496587 G_(ls)T_(ds)T_(ds)T_(ds) ^(m)C_(ds) ^(m)C_(ds)A_(ds)T_(ds)^(m)C_(ds)T_(ds)A_(ds) ^(m)C_(ds)T_(ls)A_(ls)T_(ds)T_(ls)A_(ls)A₁ 29111219029 6.3 6.0 503154 503170^(m)C_(ls)T_(ls)G_(ds)T_(ds)A_(ls)T_(ds)A_(ds) ^(m)C_(ds)A_(ds)^(m)C_(ds) ^(m)C_(ds)A_(ds)T_(ds) ^(m)C_(ds) ^(m)C_(ds) ^(m)C_(ls)A₁2912 1219030 5.0 5.0 487478 487494 A_(ls)G_(ds)T_(ds)T_(ls)^(m)C_(ls)T_(ds)A_(ds) ^(m)C_(ds)T_(ds)A_(ds)T_(ds)A_(ds)^(m)C_(ds)T_(ls)T_(ls)T_(ls) ^(m)C₁ 2913 1219031 5.3 6.0 464879 464898T_(ls)A_(ls)T_(ds)A_(ds) ^(m)C_(ls) ^(m)C_(ds)T_(ls)T_(ds)T_(ds)^(m)C_(ds)T_(ds)T_(ds)T_(ds)A_(ds)A_(ds) ^(m)C_(ds) ^(m)C_(ds)^(m)C_(ds)T_(ls)T₁ 2914 A subscript “1” indicates a 4′-2′ LNA modifiedsugar moiety, a subscript “d” indicates a 2′-β-D-deoxyribosyl sugarmoiety, a subscript “s” indicates a phosphorothioate internucleosidelinkage, and a superscript “m” before a C indicates 5-methyl cytosine.n.d. means no data; not tested.

Example 16 Tolerability of Modified Oligonucleotides Complementary toHuman UBE3A-ATS in Rats, 8 Week Study

Modified oligonucleotides described above were tested in rats to assessthe tolerability of the oligonucleotides. Groups of male, 6-8 week oldSprague Dawley rats each received a single intrathecal (IT) dose of 3 mgof oligonucleotide as listed in the table below. Each treatment groupconsisted of 4 rats, approximately matched for starting weights. A groupof four rats received PBS as a negative control. The rats were weighedbefore dosing, and were weighed again at 8 weeks post-dose. Rats areexpected to gain weight over the course of the study due to growth; toolittle weight gain is a sign of compound toxicity. Absolute values ofbody weight change were normalized to the body weight change observed inPBS-treated groups to allow comparison between two studies withdifferent average starting weights for the rats, with the body weightchange of the PBS group set to 100%. Statistical significance (p-value)of the change in body weight compared to the PBS-treated group wascalculated by a two-tailed Welch's T-test in Excel (two sample, unequalvariance). A p-value of <0.05 indicates that there is less than a 5%chance that the observed differences are due to random sampling errors.

TABLE 120 Body Weight Change in Rats at 8 weeks post-dose Compound B.W.change ID as % PBS p-value PBS 100 n/a 1219027 87 0.03 1065645 101 0.831263517 100 0.91 1263518 103 0.50 1263533 100 0.99 1273039 105 0.261273062 100 0.98

1. A modified oligonucleotide according to the following chemicalstructure:

or a salt thereof.
 2. The modified oligonucleotide of claim 1, which isthe sodium salt or the potassium salt.
 3. A modified oligonucleotideaccording to the following chemical structure:


4. A pharmaceutical composition comprising the modified oligonucleotideclaim 1 and a pharmaceutically acceptable diluent.
 5. The pharmaceuticalcomposition of claim 4, wherein the pharmaceutically acceptable diluentis artificial cerebrospinal fluid.
 6. The pharmaceutical composition ofclaim 5, wherein the pharmaceutical composition consists essentially ofthe modified oligonucleotide and artificial cerebrospinal fluid.
 7. Apharmaceutical composition comprising the modified oligonucleotide ofclaim 3 and a pharmaceutically acceptable diluent.
 8. The pharmaceuticalcomposition of claim 7, wherein the pharmaceutically acceptable diluentis artificial cerebrospinal fluid.
 9. The pharmaceutical composition ofclaim 8, wherein the pharmaceutical composition consists essentially ofthe modified oligonucleotide and artificial cerebrospinal fluid.
 10. Acompound comprising a modified oligonucleotide according to thefollowing chemical notation: A_(es) ^(m)C_(eo)^(m)C_(eo)A_(eo)T_(eo)T_(ds)T_(ds)T_(ds)G_(ds)A_(ds) ^(m)C_(ds)^(m)C_(ds)T_(ds)T_(ds) ^(m)C_(ds)T_(eo)T_(eo)A_(es)G_(es) ^(m)C_(e) (SEQID NO: 2873), wherein: A=an adenine nucleobase, mC=a 5-methyl cytosinenucleobase, G=a guanine nucleobase, T=a thymine nucleobase, e=a 2′-MOEsugar moiety, d=a 2′-β-D-deoxyribosyl sugar moiety, s=a phosphorothioateinternucleoside linkage, and o=a phosphodiester internucleoside linkage.11. A pharmaceutical composition comprising a compound of claim 10, anda pharmaceutically acceptable diluent.
 12. The pharmaceuticalcomposition of claim 11, wherein the pharmaceutically acceptable diluentis artificial cerebrospinal fluid.
 13. The pharmaceutical composition ofclaim 12, wherein the pharmaceutical composition consists essentially ofthe compound and artificial cerebrospinal fluid.
 14. The compound of 10,comprising the modified oligonucleotide covalently linked to a conjugategroup.
 15. A pharmaceutical composition comprising a compound of claim14, and a pharmaceutically acceptable diluent.
 16. The pharmaceuticalcomposition of claim 15, wherein the pharmaceutically acceptable diluentis artificial cerebrospinal fluid.
 17. The pharmaceutical composition ofclaim 16, wherein the pharmaceutical composition consists essentially ofthe compound and artificial cerebrospinal fluid.